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Kanyakumari District, India

Vijayaraghavan P.,Manonmaniam Sundaranar University | Vincent S.G.P.,International Center for Nanobiotechnology
Polish Journal of Microbiology

A microorganism hydrolyzing carboxymethyl cellulose was isolated from a paddy field and identified as Bacillus sp. Production of cellulase by this bacterium was found to be optimal at pH 6.5, 37°C and 150 rpm of shaking. This cellulase was purified to homogeneity by the combination of ammonium sulphate precipitation, DEAE cellulose, and sephadex G-75 gel filtration chromatography. The cellulase was purified up to 14.5 fold and had a specific activity of 246 U/mg protein. The enzyme was a monomeric cellulase with a relative molecular mass of 58 kDa, as determined by SDS-PAGE. The enzyme exhibited its optimal activity at 50°C and pH 6.0. The enzyme was stable in the pH range of 5.0 to 7.0 and its stability was maintained for 30 min at 50°C and its activity got inhibited by Hg2+, Cu2+, Zn2+ , Mg2+, Na2+, and Ca2+. Source

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