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Sulaiman C.T.,Center for Medicinal Plants Research | Sulaiman C.T.,Karpagam University | Gopalakrishnan V.K.,Karpagam University | Balachandran I.,Center for Medicinal Plants Research
International Journal of Phytomedicine | Year: 2012

rapid method was developed for the identification of phenolics from Acacia Catechu. The analysis of phenolic compounds was carried out by reversed-phase high-performance liquid chromatography (RPHPLC) coupled to electrospray ionization-time-of-flight mass spectrometry (ESI-Q-TOF-MS). The accurate mass data for the molecular ions were processed using the software Mass Hunter work station (Agilent Technologies), which provided a list of possible elemental formulas by using the Molecular featured extraction editor. The subsequent structure characterization was carried out by a tandem mass spectrometric method. Fragmentation behavior of phenolic compounds was investigated using ion trap mass spectrometry in negative mode. The total fragmentation of the compound ion leading to other fragments was corroborated by MS-MS. Five phenolic compounds have been identified from the methanolic extract of Acacia Catechu.


Sulaiman C.,Center for Medicinal Plants Research | Sulaiman C.,Karpagam University | Gopalakrishnan V.,Karpagam University | Balachandran I.,Center for Medicinal Plants Research
Journal of Planar Chromatography - Modern TLC | Year: 2014

Phytochemical studies were carried out in different parts of three Acacia species viz. A. catechu, A. nilotica and A. leucophloea, using chromatographic and spectrophotometric analyses. Various extracts like absolute alcohol, aqueous alcohol, hydroalcohol, and water extract, were compared both qualitatively and quantitatively. The different extracts showed that they are rich sources of phenolic compounds. The various parts, such as bark, heartwood, and leaf of A. catechu and A. nilotica, showed almost similar chemical profiles.


Sulaiman C.T.,Center for Medicinal Plants Research | Nasiya K.K.,Sree Sankara College | Balachandran I.,Center for Medicinal Plants Research
Spectroscopy Letters | Year: 2016

Acacia leucophloea is a medicinal plant used traditionally for treating many diseases. In the present study, phytochemicals were isolated from the bark of A. leucophloea using multiple chromatographic techniques. The separation and isolation were done using column chromatography. The molecular masses of isolated compounds were assigned by electro spray ionization mass spectrometry. The subsequent structure characterization was carried out by a tandem mass spectrometric method. Fragmentation behavior of compounds was investigated using hexapole collision cell mass spectrometry in negative mode. Six phenolic compounds such as catechin, leuco-fisetinidin, gallic acid, ferulic acid, syringic acid, and O-methyl epicatechin were identified. Most of the compounds identified are first report from this plant. © 2016 Taylor & Francis.


Naidoo Y.,University of KwaZulu - Natal | Karim T.,University of KwaZulu - Natal | Heneidak S.,Suez Canal University | Sadashiva C.T.,Center for Medicinal Plants Research | Naidoo G.,University of KwaZulu - Natal
Planta | Year: 2012

This study was initiated to characterize the distribution, morphology, secretion mode, histochemistry and ultrastructure of the glandular trichomes of Ceratotheca triloba using light and electron microscopy. Its leaves bear two morphologically distinct glandular trichomes. The first type has long trichome with 8-12 basal cells of pedestal, 3-14 stalk cells, a neck cell and a head of four cells in one layer. The second type has short trichome comprising one or two basal epidermal cells, a unicellular or bicellular stalk and a multicellular head of two to eight cells. There is a marked circular area in the upper part of each head cell of the long trichome. This area is provided with micropores to exudate directly the secretory product onto the leaf surface by an eccrine pathway. The secretory product has copious amount of dark microbodies arising from plastids which are positive to Sudan tests and osmium tetroxide for unsaturated lipids. The secretion mode of short trichomes is granulocrine and involves two morphologically and histochemically distinct vesicle types: small Golgi-derived vesicles which are positive to Ruthenium Red test for mucilaginous polysaccharides; the second type is dark large microbodies similar to that of long trichomes with low quantity. These two types are stored in numerous peripheral vacuoles and discharge their contents accompanied by the formation of irregular invaginations of the plasmalemma inside the vacuoles via reverse pinocytosis. These two secretion modes of long and short trichomes are reported for the first time in the family Pedaliaceae. The long trichomes have more unsaturated lipids, while the short trichomes contain more mucilaginous polysaccharides. © 2012 Springer-Verlag.


PubMed | Indian Institute of Spices Research, Providence College, Center for Medicinal Plants Research, World Noni Research Foundation and Central Plantation Crops Research Institute
Type: | Journal: Methods in molecular biology (Clifton, N.J.) | Year: 2016

Ginger is a rhizomatous plant that belongs to the family Zingiberaceae. It is a herbaceous perennial but cultivated as annual, with crop duration of 7-10 months. Ginger is native to India and Tropical South Asia. The tuberous rhizomes or underground stems of ginger are used as condiment, an aromatic stimulant, and food preservative as well as in traditional medicine. Ginger is propagated vegetatively with rhizome bits as seed material. Cultivation of ginger is plagued by rhizome rot diseases, most of which are mainly spread through infected seed rhizomes. Micropropagation will help in production of disease-free planting material. Sexual reproduction is absent in ginger, making recombinant breeding very impossible. In vitro technology can thus become the preferred choice as it can be utilized for multiplication, conservation of genetic resources, generating variability, gene transfer, molecular tagging, and their utility in crop improvement of these crops.


Raghu A.V.,Center for Medicinal Plants Research | Geetha S.P.,Center for Medicinal Plants Research | Martin G.,Center for Medicinal Plants Research | Balachandran I.,Center for Medicinal Plants Research | Mohanan K.V.,University of Calicut
Indian Journal of Natural Products and Resources | Year: 2010

A simple and efficient method for high frequency direct shoot regeneration from nodal explants of Tribulus terrestris Linn. (Family - Zygophyllaceae), a medicinally important plant is described in this paper. Woody Plant Medium (WPM) supplemented with 4.0 mg/l BA was found to be most effective in inducing bud break, growth and also in initiating multiple shoot proliferation at the rate of six to seven micro-shoots per nodal explant after four weeks of culture. A high frequency multiplication rate was established by repeated sub-culturing. Excised shoots were rooted on MS basal medium without any growth regulators and got established after a hardening process with 80% survival. Micropropagated plants grew well under field conditions, attained maturity and flowered. No phenotypical differences were observed among regenerated plants.


Sulaiman C.T.,Center for Medicinal Plants Research | Balachandran I.,Center for Medicinal Plants Research
Natural Product Research | Year: 2016

Characterisation of antioxidant fraction of the fruit of Garcinia gummi-gutta was done using liquid chromatography mass spectroscopic (LC/MS) analyses. Total poly phenolics and radical scavenging activity of various extracts such as acetone, methanol and hydroalcohol were estimated spectrophotometrically. The active extract was analysed by LC/MS in order to identify the molecular mass and tentative structures of major compounds. Phenolic compounds such as luteolin 7-O-glucuronide, kaempferol 3-O-(6-O-acetyl) glycoside, dicaffeoylquinic acid, apigenein-6-C-pentosyl-8-C-hexoside and p-coumarylquinic acid were identified from hydro alcoholic extract of G. gummi-gutta. © 2016 Informa UK Limited, trading as Taylor & Francis Group


Sulaiman C.T.,Center for Medicinal Plants Research | Balachandran I.,Center for Medicinal Plants Research
Spectroscopy Letters | Year: 2015

Amruthotharam kashayam is an important Ayurvedic formulation prepared using specified plant parts of Tinospora cordifolia, Terminalia chebula, and Zingiber officinale. The current study developed a rapid liquid chromatographic method coupled with electro spray ionization mass spectrometry for the identification of major phytoconstituents present in the formulation. Reverse phase high-pressure liquid chromatogram was developed as chemical fingerprint. The mass spectrum along with the MS/MS fragmentation on collision-induced dissociation led to the structural identification of separated compounds. Phenolic acids such as quinic acid, protocatechuic acid, gallic acid, and chebulic acid were identified in the formulation along with some flavonoids. © 2015 Taylor & Francis Group, LLC.


PubMed | Indian Institute of Spices Research, Providence College, World Noni Research Foundation and Center for Medicinal Plants Research
Type: | Journal: Methods in molecular biology (Clifton, N.J.) | Year: 2016

Turmeric is a rhizomatous herbaceous perennial but cultivated as annual, belonging to the family Zingiberaceae. It is a native of India and South East Asia. The tuberous rhizomes or underground stems of turmeric are used from antiquity as condiments, a dye and as an aromatic stimulant in several medicines. Turmeric is an important crop in India and it is used as a spice, food preservative, coloring agent, cosmetic as well as for its medicinal properties. Propagation is done vegetatively with rhizome bits as seed materials. It is plagued by rhizome rot diseases most of which are mainly spread through infected seed rhizomes. Micropropagation will help in production of disease-free seed. Sexual reproduction is rare in turmeric, making recombinant breeding very difficult. In vitro technology can thus become the preferred choice and it can be utilized for multiplication, conservation of genetic resources, generating variability, gene transfer, molecular tagging, and their utility in crop improvement.


PubMed | Center for Medicinal Plants Research
Type: | Journal: Natural product research | Year: 2016

Characterisation of antioxidant fraction of the fruit of Garcinia gummi-gutta was done using liquid chromatography mass spectroscopic (LC/MS) analyses. Total poly phenolics and radical scavenging activity of various extracts such as acetone, methanol and hydroalcohol were estimated spectrophotometrically. The active extract was analysed by LC/MS in order to identify the molecular mass and tentative structures of major compounds. Phenolic compounds such as luteolin 7-O-glucuronide, kaempferol 3-O-(6-O-acetyl) glycoside, dicaffeoylquinic acid, apigenein-6-C-pentosyl-8-C-hexoside and p-coumarylquinic acid were identified from hydro alcoholic extract of G. gummi-gutta.

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