Center for Liver Research and Diagnostics

Hyderabad andhra Pradesh, India

Center for Liver Research and Diagnostics

Hyderabad andhra Pradesh, India
SEARCH FILTERS
Time filter
Source Type

Bardia A.,Center for Liver Research and Diagnostics | Tiwari S.K.,Center for Liver Research and Diagnostics | Gunisetty S.,Center for Liver Research and Diagnostics | Anjum F.,Center for Liver Research and Diagnostics | And 3 more authors.
Inflammation Research | Year: 2012

Objective The present study was designed to investigate the role of X-ray cross-complementing group 1 (XRCC1) and apurinic/apyrimidinic endonuclease 1 (APE1) polymorphisms in apoptosis and the risk of ulcerative colitis (UC). Materials and methods Blood samples from 384 unrelated subject (age range 18-65 years; 171 with UC, 213 healthy controls) were collected after colonoscopy. Genomic DNA was isolated and genotyped for XRCC1 Arg399Gln and APE1 Asp148Glu using a confronting twopair primers polymerase chain reaction. Apoptosis and intracellular reactive oxygen species (ROS) levels in peripheral blood mononuclear cells were measured using annexin-V and H 2DCFDA assay, respectively. Results The frequency of genotype Arg399Gln (heterozygous) of XRCC1 gene was significantly higher in patients with UC than the controls (odds ratio [OR] 1.73; 95% confidence interval [CI] 1.13-2.64; p = 0.01). Similarly the genotypic frequency of APE1 Asp148Glu showed statistically significant incidence among UC subjects (OR 1.54; 95% CI 1.02-2.33; p = 0.04). Polymorphism in XRCC1 Arg399Gln and APE1 Asp148Glu together considerably increased the risk of UC (OR 2.303; 95% CI 1.43-3.69; p = 0.0007). ROS levels were high in UC subjects compared with controls (p = 0.01). Conclusion Polymorphisms in XRCC1 Arg399Gln and APE1 Asp148Glu significantly increased the rate of apoptosis and risk of ulcerative colitis. Copyright © Springer Basel AG 2011.


Khan A.A.,Center for Liver Research and Diagnostics | Shaik M.V.,Center for Liver Research and Diagnostics | Parveen N.,Center for Liver Research and Diagnostics | Rajendraprasad A.,Center for Liver Research and Diagnostics | And 9 more authors.
Cell Transplantation | Year: 2010

Liver transplantation is the only existing modality for treating decompensated liver cirrhosis. Several factors, such as nonavailability of donors, combined with operative risks, complications associated with rejection, usage of immunosuppressive agents, and cost intensiveness, make this strategy available to only a few people. With a tremendous upsurge in the mortality rate of patients with liver disorders worldwide, there is a need to search for an alternative therapeutic tool that can combat the above limitations and serve as a supportive therapy in the management of liver diseases. Cell therapy using human fetal liver-derived stem cells can provide great potential to conservatively manage end-stage liver diseases. Therefore, the present investigation aimed to study and prove the safety and efficacy of human fetal liver-derived stem cell transplantation in patients with end-stage liver cirrhosis. Twenty-five patients with liver cirrhosis of different etiologies were infused with human fetal liver-derived stem cells (EpCAM+ve) labeled with Tc-HMPAO through hepatic artery. Our high throughput analysis using flow cytometry, RT-PCR, and cellular characterization exemplifies fetal liver cells with their high proliferation rate could be the best source for rejuvenating the diseased liver. Further, no episodes related to hepatic encephalopathy recurred in any of the subjects following hepatic stem cell transplantation. There was marked clinical improvement observed in terms of all clinical and biochemical parameters. Further, there was decrease in mean MELD score (p < 0.01) observed in 6 months follow-up in all patients. Therapy using human fetal liver stem/progenitor cells offers a potentially supportive modality to organ transplantation in the management of liver diseases. Copyright © 2010 Cognizant Comm. Corp.


Khan A.A.,Center for Liver Research and Diagnostics | Khan A.A.,Princess Esra Hospital | Vishwakarma S.K.,Center for Liver Research and Diagnostics | Bardia A.,Center for Liver Research and Diagnostics
Journal of Artificial Organs | Year: 2014

Demand of donor organs for transplantation in treatment of organ failure is increasing. Hence there is a need to develop new strategies for the alternative sources of organ development. Attempts are being made to use xenogenic organs by genetic manipulation but the organ rejection against human always has been a major challenge for the survival of the graft. Advancement in the genetic bioengineering and combination of different allied sciences for the development of humanized organ system, the therapeutic influence of stem cell fraction on the reconstitution of organ architecture and their regenerative abilities in different tissues and organs provides a better approach to solve the problem of organ shortage. However, the available strategies for generating the organ/tissue scaffolds limit its application due to the absence of complete three-dimensional (3D) organ architecture, mechanical strength, long-term cell survival, and vascularization. Repopulation of whole decellularized organ scaffolds using stem cells has added a new dimension for creating new bioengineered organs. In recent years, several studies have demonstrated the potential application of decellularization and recellularization approach for the development of functional bio-artificial organs. With the help of established procedures for conditioning, extensive stem cells and organ engineering experiments/transplants for the development of humanized organs will allow its preclinical evaluation for organ regeneration before translation to the clinic. This review focuses on the major aspects of organ scaffold generation and repopulation of different types of whole decellularized organ scaffolds using stem cells for the functional benefit and their confines. © 2014, The Japanese Society for Artificial Organs.


Tiwari S.K.,Center for Liver Research and Diagnostics | Manoj G.,Center for Liver Research and Diagnostics | Kunwar A.,Bhabha Atomic Research Center | Amrita N.,Center for Liver Research and Diagnostics | And 4 more authors.
Scandinavian Journal of Infectious Diseases | Year: 2010

We examined sodium selenite, an inorganic selenium supplement, for its ulcer healing properties and antimicrobial activity against gastric pathogen Helicobacter pylori. Minimum inhibitory concentrations (MIC) were determined using disk diffusion and flow cytometry. The studies were performed over a concentration range of 1 μg/ml to 500 μg/ml sodium selenite. Mild activity was seen at 10 μg/ml and 50 μg/ml, a moderate response at 100 μg/ml and strong response at 500 μg/ml with a MIC value of 10 μg/ml. The compound was found to be active at low pH without any resistance after 10 passages. Flow cytometry data showed a characteristic shift of the viability peak in comparison with the control, thereby confirming the bactericidal effects of sodium selenite. Sodium selenite administered in Wistar rats, pre-ulcerated with naproxen and infected with H. pylori, showed ulcer healing and anti-H. pylori activity at a concentration range of 1050 μg/rat; however concentrations of 100 μg/rat and 500 μg/rat were found to be toxic in the in vivo studies. In conclusion, sodium selenite shows both ulcer healing and anti-H. pylori activity at a low concentration (10 μg/rat) without toxicity. © 2010 Informa UK Ltd.


Zeeyauddin K.,Center for Liver Research and Diagnostics | Setty R.C.S.,Center for Liver Research and Diagnostics | Majid Mufaqam S.A.,Center for Liver Research and Diagnostics | Ibrahim M.,Center for Liver Research and Diagnostics
Indian Drugs | Year: 2010

The effect of petroleum ether and ethanolic leaf extract of plant Boswellia serrata (Family-Burseraceae) was evaluated in paracetamol induced hepatotoxicity (3 g per kg) in albino rats. The liver damage was evidenced by elevated levels of serum bilirubin, serum glutamate pyruvate transaminase (SGPT), serum glutamate oxalo acetate transaminase (SGOT), reduced level of total proteins and by histopathological observations of liver sections. The hepatoprotective activity was found to be more pronounced in petroleum ether leaf extract (250 mg / kg) of Boswellia serrata plant as it significantly reduced these elevated levels of serum bilirubin, SGPT, SGOT and increased the levels of total proteins. Histopathological observations also confirm the hepatoprotective activity of Boswellia serrata leaf extracts in albino rats.


Sivaram G.,Center for Liver Research and Diagnostics | Tiwari S.K.,Center for Liver Research and Diagnostics | Bardia A.,Center for Liver Research and Diagnostics | Anjum F.,Center for Liver Research and Diagnostics | And 3 more authors.
Human Immunology | Year: 2012

Ulcerative colitis is a multifactorial disease in which genetic factors play a major role. Functional mutations in the genes related to innate immune response exacerbate mucosal damage coupled with persistent inflammation. The cytokine macrophage migration inhibitory factor (MIF), CD14, and Toll-like receptor 4 (TLR4) are the central players with clearly defined roles in inflammation. The aim of this study was to investigate the association between MIF-173G > C, CD14-159C > T, and TLR4-299A > G polymorphisms and mononuclear cell expression in patients with ulcerative colitis (UC). Genotyping of MIF-173G > C, CD14-159C > T, and TLR4-299A > G polymorphisms was performed by amplification refractory mutation system-polymerase chain reaction and allele-specific amplification in 139 and 176 patients with UC and controls, respectively. Simultaneously, the expression levels of intracellular MIF, mCD14, and mTLR4 were determined in mononuclear cells using a flow cytometer. Polymorphisms in CD14-159C > T and TLR4-299A > G significantly affected mCD14 and mTLR4 expression levels and also increased susceptibility to UC. Although intracellular MIF expression levels differed among patient and control groups, the polymorphism in MIF 173G > C was not observed to be associated with a risk of UC. © 2012 American Society for Histocompatibility and Immunogenetics.


PubMed | Center for Liver Research and Diagnostics
Type: Journal Article | Journal: Journal of artificial organs : the official journal of the Japanese Society for Artificial Organs | Year: 2014

Demand of donor organs for transplantation in treatment of organ failure is increasing. Hence there is a need to develop new strategies for the alternative sources of organ development. Attempts are being made to use xenogenic organs by genetic manipulation but the organ rejection against human always has been a major challenge for the survival of the graft. Advancement in the genetic bioengineering and combination of different allied sciences for the development of humanized organ system, the therapeutic influence of stem cell fraction on the reconstitution of organ architecture and their regenerative abilities in different tissues and organs provides a better approach to solve the problem of organ shortage. However, the available strategies for generating the organ/tissue scaffolds limit its application due to the absence of complete three-dimensional (3D) organ architecture, mechanical strength, long-term cell survival, and vascularization. Repopulation of whole decellularized organ scaffolds using stem cells has added a new dimension for creating new bioengineered organs. In recent years, several studies have demonstrated the potential application of decellularization and recellularization approach for the development of functional bio-artificial organs. With the help of established procedures for conditioning, extensive stem cells and organ engineering experiments/transplants for the development of humanized organs will allow its preclinical evaluation for organ regeneration before translation to the clinic. This review focuses on the major aspects of organ scaffold generation and repopulation of different types of whole decellularized organ scaffolds using stem cells for the functional benefit and their confines.


PubMed | Center for Liver Research and Diagnostics
Type: Journal Article | Journal: Journal of advanced research | Year: 2015

Since last few years, an impressive amount of data has been generated regarding the basic in vitro and in vivo biology of neural stem cells (NSCs) and there is much far hope for the success in cell replacement therapies for several human neurodegenerative diseases and stroke. The discovery of adult neurogenesis (the endogenous production of new neurons) in the mammalian brain more than 40years ago has resulted in a wealth of knowledge about stem cells biology in neuroscience research. Various studies have done in search of a suitable source for NSCs which could be used in animal models to understand the basic and transplantation biology before treating to human. The difficulties in isolating pure population of NSCs limit the study of neural stem behavior and factors that regulate them. Several studies on human fetal brain and spinal cord derived NSCs in animal models have shown some interesting results for cell replacement therapies in many neurodegenerative diseases and stroke models. Also the methods and conditions used for in vitro culture of these cells provide an important base for their applicability and specificity in a definite target of the disease. Various important developments and modifications have been made in stem cells research which is needed to be more specified and enrolment in clinical studies using advanced approaches. This review explains about the current perspectives and suitable sources for NSCs isolation, characterization, in vitro proliferation and their use in cell replacement therapies for the treatment of various neurodegenerative diseases and strokes.


PubMed | King Saud University, Osmania University and Center for Liver Research and Diagnostics
Type: Journal Article | Journal: PloS one | Year: 2014

Ulcerative colitis (UC) is a major clinical form of inflammatory bowel disease. UC is characterized by mucosal inflammation limited to the colon, always involving the rectum and a variable extent of the more proximal colon in a continuous manner. Genetic variations in DNA repair genes may influence the extent of repair functions, DNA damage, and thus the manifestations of UC. This study thus evaluated the role of polymorphisms of the genes involved in DNA repair mechanisms. A total of 171 patients and 213 controls were included. Genotyping was carried out by ARMS PCR and PCR-RFLP analyses for RAD51, XRCC3 and hMSH2 gene polymorphisms. Allelic and genotypic frequencies were computed in both control & patient groups and data was analyzed using appropriate statistical tests. The frequency of A allele of hMSH2 in the UC group caused statistically significant increased risk for UC compared to controls (OR 1.64, 95% CI 1.16-2.31, p = 0.004). Similarly, the CT genotype of XRCC3 gene was predominant in the UC group and increased the risk for UC by 1.75 fold compared to controls (OR 1.75, 95% CI 1.15-2.67, p = 0.03), further confirming the risk of T allele in UC. The GC genotype frequency of RAD51 gene was significantly increased (p = 0.02) in the UC group (50.3%) compared to controls (38%). The GC genotype significantly increased the risk for UC compared to GG genotype by 1.73 fold (OR 1.73, 95% CI 1.14-2.62, p = 0.02) confirming the strong association of C allele with UC. Among the controls, the SNP loci combination of hMSH2:XRCC3 were in perfect linkage. The GTC and ACC haplotypes were found to be predominant in UC than controls with a 2.28 and 2.93 fold significant increase risk of UC.


PubMed | Center for Liver Research and Diagnostics
Type: Journal Article | Journal: Gastroenterology research | Year: 2016

Idiopathic thrombocytopenic purpura (ITP) is an immune-mediated thrombocytopenia. ITP is the result of accelerated platelet destruction by the reticuloendothelial system, primarily the spleen. The prevalence of

Loading Center for Liver Research and Diagnostics collaborators
Loading Center for Liver Research and Diagnostics collaborators