3National Center for Genetic Engineering and Biotechnology

Pathum Thani, Thailand

3National Center for Genetic Engineering and Biotechnology

Pathum Thani, Thailand
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PubMed | 3National Center for Genetic Engineering and Biotechnology, Chulalongkorn University and RIKEN
Type: Journal Article | Journal: International journal of systematic and evolutionary microbiology | Year: 2016

Five actinomycete isolates, CPB1-1T, CPB2-10, BM1-4, CPB3-1 and CPB1-18, belonging to the genus Streptomyces were isolated from marine sediments collected from Chumphon Province, Thailand. They produced open loops of warty spore chains on aerial mycelia. ll-Diaminopimelic acid, glucose and ribose were found in their whole-cell hydrolysates. Polar lipids found were diphosphatidylglycerol, phosphatidylethanolamine, lysophosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositol and phosphatidylinositol mannoside. Menaquinones were MK-9(H6), MK-9(H8), MK-10(H6) and MK-10(H8). Major cellular fatty acids were anteiso-C15:0, anteiso-C17:0 and iso-C16:0. The taxonomic position of the strains was described using a polyphasic approach. blastn analysis of the 16S rRNA gene sequence revealed that these five strains exhibited the highest similarities with Streptomyces mangrovicola GY1 (99.0%), Streptomyces fenghuangensisGIMN4.003T (98.6%), Streptomyces barkulensisRC 1831T (98.5%) and Streptomyces radiopugnans R97T (98.3%). However, their phenotypic characteristics and 16S rRNA gene sequences as well as DNA-DNA relatedness differentiated these five strains from the other species of the genus Streptomyces. Here, we propose the novel actinomycetes all being representatives of the same novel species, Streptomyces verrucosisporus, with type strain CPB1-1T (=JCM 18519T=PCU 343T=TISTR 2344T).


PubMed | 3National Center for Genetic Engineering and Biotechnology, 2National Center for Genetic Engineering and Biotechnology, 4National Center for Genetic Engineering and Biotechnology and 1National Center for Genetic Engineering and Biotechnology
Type: | Journal: International journal of systematic and evolutionary microbiology | Year: 2015

A novel filamentous bacterial strain, which developed irregular sporangia at the end of sporangiophores on substrate mycelia, was isolated from dry evergreen forest soil collected in Thailand. The 16S rRNA gene sequence and phylogenetic analysis indicated that designated strain A-T 5190T, belonged to the genus Actinoplanes and was most closely related to A. palleronii NBRC 14916T (98.88%) and A. rectilineatus NBRC 13941T (98.54%). The DNA-DNA relatedness values that distinguished strain A-T 5190T from the closest species were below 70%. The cell-wall peptidoglycan contained meso-diaminopimelic acid. The whole-cell sugars contained rhamnose, ribose, galactose and xylose. The predominant menaquinone was MK-9(H4). The diagnostic phospholipids were phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The predominant cellular fatty acids were unsaturated fatty acids C17:1 and branched fatty acids iso-C16:0, iso-C15:0 and anteiso-C17:0. The G + C content of the genomic DNA was 71.9 mol%. Following an evidence of phenotypic, chemotaxonomic and genotypic studies, the new isolate is proposed as a novel species to be named Actinoplanes luteus sp. nov. The type strain is A-T 5190T (= BCC 41582T = NBRC 109644T).

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