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Belgrade, Serbia

Tomasevic I.,University of Belgrade | Kuzmanovic J.,Center for Food Analysis | Andelkovic A.,Center for Food Analysis | Saracevic M.,Center for Food Analysis | And 2 more authors.
Meat Science | Year: 2016

A total of 48,246 microbiological test results were collected from 130 meat processing plants and 220 meat retail facilities over a seven year period: 41months before and 43months after HACCP implementation. Our results confirm a strong positive effect of mandatory HACCP implementation on process hygiene indicators in meat establishments. Significant reductions were observed in the number of hygiene indicator organisms on all types of surfaces examined and types of meat establishments investigated. The improvement of process hygiene was articulated as aerobic colony count reduction of at least 1.0log10CFU/cm2 for food contact surfaces and over 2log10CFU/cm2 for cooling facilities (refrigerators, freezers and other meat cooling devices). Meat handlers' hands hygiene was least positively affected. The period after mandatory HACCP implementation was also marked by a steady decline of positive Enterobacteriaceae and Staphylococcus samples. Process hygiene advances for meat processing plants and meat retail facilities were similar. © 2015 Elsevier Ltd.

Tomasevic I.,University of Belgrade | Petrovic J.,Center for Food Analysis | Jovetic M.,Center for Food Analysis | Raicevic S.,Center for Food Analysis | And 2 more authors.
Food Control | Year: 2015

The incidence of contamination of aflatoxin M1 (AFM1) in milk and milk products samples collected in Serbia was investigated by using the competitive enzyme linked immunosorbent assay (ELISA) technique. A total of 1438 samples composed of 678 raw milk, 438 heat treated milk and 322 milk product samples that were analyzed during the period of 2013-2014, including all seasons. The AFM1 levels exceeded the European Union maximum residue permitted amount (EU MRL) in 56.3% raw milk, 32.6% heat treated milk and 37.8% of milk product samples. Milk powders had the highest mean AFM1 concentration (0.847μg/kg) of all types of milk products examined. Mean concentration of AFM1 in raw milk samples during the period of winter in Serbia was 0.358μg/kg and did not significantly differ from the mean concentrations of 0.375μg/kg during the spring. However, the AFM1 raw milk concentration in the summer (0.039μg/kg) and autumn season (0.103μg/kg) was significantly lower. Seasonal variation of AFM1 concentrations in heat treated milk samples followed the trend observed in raw milk. Mean raw milk AFM1 concentration has dropped down by 10 fold from 0.314μg/kg in 2013 to 0.035μg/kg in 2014. The fraction of raw milk samples exceeding the EU MRL has decreased from 62.3% to 11.5% by the end of 2014. © 2015 Elsevier Ltd.

Lazarevic K.B.,Center for Food Analysis | Andric F.,University of Belgrade | Trifkovic J.,University of Belgrade | Tesic Z.,University of Belgrade | Milojkovic-Opsenica D.,University of Belgrade
Food Chemistry | Year: 2012

The characterisation of three unifloral Serbian honeys (acacia, sunflower and linden) was carried out based on some common physicochemical parameters (water content, electrical conductivity, free acidity, optical rotation and pH). A total of 201 honey samples, collected during the 2009 harvesting season, were analysed. Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to highlight the data structure and to find the relationships between the physicochemical parameters and the botanical origin of honey. The variables that best discriminated the samples were electrical conductivity (ranging from 0.10 to 0.76 mS/cm), free acidity (ranging from 7.80 to 42.70 meq/kg) and pH (ranging from 3.17 to 5.85). LDA resulted in a classification model with a high predictive power, allowing further assessment of unknown samples of the three unifloral honeys. Determination of geographic origin of acacia honey samples based on physicochemical properties and chemometrics was attempted. © 2011 Elsevier Ltd. All rights reserved.

Kolundzic M.,University of Belgrade | Grozdanic N.D.,Serbian Institute for Oncology and Radiology of Serbia | Dodevska M.,Center for Food Analysis | Milenkovic M.,University of Belgrade | And 4 more authors.
Industrial Crops and Products | Year: 2016

The antibacterial activity of cyclohexane, dichlormethane, methanol and aqueous extracts of tinder fungus Fomes fomentarius (L.) Fr (Polyporaceae) was tested against 9 bacterial strains (Staphylococcus aureus, Staphylococcus epidermidis, Micrococcus luteus, Bacillus subtilis, Enterococcus feacalis, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Salmonella abony), as well as against 10 different clinical isolates and one reference strain of Helicobacter pylori. Minimal inhibitory concentrations (MICs) of all extracts against 9 bacterial strains were in the range of 125-250μg/ml. Methanol and aqueous extracts showed significant activity against H. pylori with MIC values between 4-32μg/ml. Also, cytotoxicity of tested extracts was significant. Aqueous extract was the most active one against HeLa cells with an IC50 8.31±1.18μg/ml and N87 cells with IC50 64.46±3.13μg/ml without any activity against normal MRC5 cell line (>200μg/ml). © 2015 Elsevier B.V.

Keckes J.,Institute of Veterinary Medicine of Serbia | Trifkovic J.,University of Belgrade | Andric F.,University of Belgrade | Jovetic M.,Center for Food Analysis | And 2 more authors.
Journal of the Science of Food and Agriculture | Year: 2013

BACKGROUND: The free amino acids profile of 192 samples of seven different floral types of Serbian honey (acacia, linden, sunflower, rape, basil, giant goldenrod, and buckwheat) from six different regions was analysed in order to distinguish honeys by their botanical origin. RESULTS: The most abundant amino acids were proline, alanine, phenylalanine, threonine and arginine. Based on the established amino acids profiles, some important differences have been identified among studied honey samples relying on the basic descriptive statistics data, and confirmed by multivariate chemometric methods. Principal component analysis revealed that basil honey samples form a well-defined cluster imposed with phenylalanine content. The model obtained by linear discriminant analysis might be used to distinguish basil honey from the rest of the samples, and has moderate predictive power to separate genuine acacia, linden, sunflower and rape honeys. New data for the amino acids profile of giant goldenrod and buckwheat honey samples are presented. CONCLUSIONS: The floral origin of honey could be successfully evaluated by its amino acids profile coupled with chemometric analysis. © 2013 Society of Chemical Industry.

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