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Zhang H.,Anhui Medical University | Qi P.,Anhui Medical University | Si S.-Y.,Center for Experimental Medicine | Ma W.-M.,Anhui Medical University
Chinese Journal of Cancer Prevention and Treatment | Year: 2013

OBJECTIVE: To study the effects of infrasound exposure on colon cancer in BALB/c mice. METHODS: Mice colon cancer cell CT26 was cultured, and divided to three groups: control group, 8 Hz/130 dB and 16 Hz/130 dB groups. In vivo, CT26 cells were subcutaneously injected in male BALB/c mice and also divided to three groups: control group, 8 Hz/100 dB and 16 Hz/100 dB groups. Every group both in vitro and in vivo was exposed to infrasound environment 2 h a day for 7 days. After infrasound exposure, proliferation activity of cultured CT26 cells was measured in vitro by MTT method. Blood specimen was obtained from mice eyeball and transplanted tumor was removed and tumor volume and weight were measured. The inhibition rate was calculated; the expression of Caspase-3 protein were measured by the immunhistochemistry assay; serum CEA was measured in vivo by ELISA method. RESULTS: 1)Compared with controls, the proliferation activity in vitro was lower in 8 Hz/130 dB and 16 Hz/130 dB group than that in control group (P<0.05). 2)Necrosis of transplanted tumor cells in vivo was found in 8 Hz/100 dB and 16 Hz/100 dB groups. 3)Caspase-3 clored with bromnish and was higher expression in 16 Hz/100 dB group than that in control group in vivo(P<0.05). Serum CEA level was lower in 16 Hz/100 dB group(1901.92±362.82) ρg/mL than that in control group (3929.14±2934.33) ρg/mL in vivo (P=0.041). CONCLUSIONS: The growth of CT26 in vitro can be inhibited after 8 Hz/130 dB and 16 Hz/130 dB infrasound exposure. Necrosis of transplanted tumor cells can be caused after 8 Hz/100 dB and 16 Hz/100 dB infrasound exposure. Apoptosis of transplanted tumor cells and decrease serum CEA level can be promoted after 16 Hz/100 dB infrasound exposure. Source

Nowacka M.M.,Medical University of Silesia, Katowice | Nowacka M.M.,Academy of Physical Education in Katowice | Nowacka M.M.,Center for Experimental Medicine | Paul-Samojedny M.,Medical University of Silesia, Katowice | And 2 more authors.
Neuroscience Research | Year: 2014

The aim of the present study was to estimate the influence of antidepressants given chronically on brain-derived neurotrophic factor (BDNF) alterations induced by lipopolysaccharide (LPS) in the amygdala and hippocampus of female rats subjected to chronic social instability stress (CSIS) for 29-30 days. CSIS was used as a paradigm known to be more stressful for females because stress induces affective disorders more frequently in women than men. An increased relative adrenal weight and a tendency towards the enhanced plasma corticosterone concentration were found in the stressed rats. Sucrose preference was not changed. On the last experimental day, the rats in the estrus phase were injected ip with LPS (1. mg/kg). In the stressed rats, LPS administration decreased BDNF mRNA levels in both limbic structures. Desipramine (10. mg/kg), fluoxetine (5. mg/kg) or tianeptine (10. mg/kg) given ip once daily reversed the effect of the combined stress and LPS, and tianeptine induced the strongest effects. These results indicate that chronic stress enhances vulnerability of BDNF response to deleterious influence of neuroinflammation in the examined limbic structures, what may account for its role in triggering neuropsychiatric diseases. The observed effect of antidepressants may be of significance for their therapeutic effects in the stress-induced affective disorders in females. © 2014 Elsevier Ireland Ltd and the Japan Neuroscience Society. Source

Deng H.,Center for Experimental Medicine | Deng H.,Central South University | Tan T.,Center for Experimental Medicine
Current Genomics | Year: 2015

Syndactyly, webbing of adjacent digits with or without bony fusion, is one of the most common hereditary limb malformations. It occurs either as an isolated abnormality or as a component of more than 300 syndromic anomalies. There are currently nine types of phenotypically diverse non- syndromic syndactyly. Non-syndromic syndactyly is usually inherited as an autosomal dominant trait, although the more severe presenting types and subtypes may show autosomal recessive or X-linked pattern of inheritance. The phenotype appears to be not only caused by a main gene, but also depend- n_ Deng ant on genetic background and subsequent signaling pathways involved in limb formation. So far, the principal genes identified to be involved in congenital syndactyly are mainly involved in the zone of polarizing activity and sonic hedgehog pathway. This review summarizes the recent progress made in the molecular genetics, including known genes and loci responsible for non-syndromic syndactyly, and the signaling pathways those genetic factors involved in, as well as clinical features and animal models. We hope our review will contribute to the understanding of underlying pathogenesis of this complicated disorder and have implication on genetic counseling. © 2015 Bentham Science Publishers Source

Schuck O.,Transplant Center | Teplan V.,Transplant Center | Franekova J.,Specialized Laboratory of Biochemistry | Malinska H.,Center for Experimental Medicine | And 4 more authors.
Clinical Nephrology | Year: 2014

It is not yet clear whether or not renal function in the living donor can be sufficiently assessed by estimated glomerular filtration rate (GFR) using creatinine-based equations. The present paper investigates the relationship between GFR values determined using renal inulin clearance (Cin) and those estimated using the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) formula. Our study was performed in 287 potential kidney donors with a mean age of 48 ± 10 years. Mean Cin was 1.47 ± 0.28 (1.10 - 2.50) mL/s/1.73 m2. Total bias when using the CKDEPI formula was -0.0183 mL/s/1.73 m2, precision 0.263 mL/s/1.73 m2, and accuracy 90.6% within ± 30% of Cin. The sensitivity of CKD-EPI to estimate a decrease in Cin below 1.33 mL/s/1.73 m2 was 50.5%, with an 85% specificity of detecting a value above the cutoff. Receiver-operating curve analysis for the above produced an area under the curve of 0.766 ± 0.0285 (CI 0.712 - 0.813). For donor screening purposes, CKD-EPI should be interpreted with great caution. © 2014 Dustri-Verlag Dr. K. Feistle. Source

Liu K.,306 Hospital of PLA | Feng K.,Center for Experimental Medicine | Wang P.,306 Hospital of PLA | Jia H.-Y.,Physical Examination Center | And 3 more authors.
Medical Journal of Chinese People's Liberation Army | Year: 2013

Objective To explore the relationship between aquaporin-5 (AQP5) gene polymorphism and the genetic susceptibility and clinical severity of chronic obstructive pulmonary disease (COPD) in Han population of north China. Methods Two hundred and twenty COPD patients (142 males and 78 females, aged 37-101 (74.0±10.0) years, diagnosed in the 306 Hospital of PLA from Jun. 2007 to May 2012) were listed as the study group, and 285 healthy subjects (183 males and 102 females, aged 44- 97 (72.5±8.6) years) were recruited as control group. The COPD patients with FEV1/FVC <70% were divided into 4 subgroups based on Post-Bronchodiflator FEV1: those with FEV1% ≥80% were assigned as mild subgroup, patients with 50% ≤FEV1% <80% as in moderate subgroup, with 30% ≤FEV1% <50% as severe subgroup, and those with FEV1% ≤30% as very severe subgroup. Patients in mild and moderate subgroups were combined into mild-to-moderate group, and those in severe and very severe subgroups were combined into severe-to-very severe group. Three tSNPs (rs3736309, rs296754 and rs296759) of AQP5 gene were selected for genotyping by PCR-RFLP and SNaPshot analysis. Chi-square test was used to perform the Hardy-Weinberg test, and unconditional logistic regression was used to calculate the odds ratio (OR) and 95% confidence interval (CI). The genotype frequency distribution was compared to identify relationship between AQP5 gene polymorphisms and COPD in Han population of north China. The genotype frequency distribution between mild-to-moderate and severe-to-very severe group was compared to further clarify the relationship between AQP5 gene polymorphisms and the severity of COPD. Results The genotype distribution of 3 tSNPs in COPD patients showed no difference from that in healthy controls. Advanced analysis performed in the COPD patients revealed that the genotype distribution of rs3736309 in mild-to-moderate COPD patients was significantly different to that in severe-tovery severe patients (P<0.05). The genotypes rs296754 and rs296759 were not associated with severity of airway obstruction. Conclusion The AQP5 polymorphism is not associated with the incidence of COPD, but associated with a high risk of developing severe of COPD. Source

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