Biohumoral and endocrine parameters in assessment of surgical trauma in open and laparoscopic cholecystectomy [Metabolički i endokrini parametri u proceni hirurške traume kod otvorene i laparoskopske holecistektomije]
Radunovic M.,University of Montenegro |
Radunovic M.,General Hospital Berane |
Lazovic R.,University of Montenegro |
Panic N.,University of Belgrade |
And 3 more authors.
Vojnosanitetski Pregled | Year: 2013
Background/Aim. Every surgical procedure causes metabolic, endocrine, and hemodynamic stress in the organism. The aim of this work was to assess the extent of trauma following each of the two types of cholecystectomy (traditional/open and laparoscopic) by measuring palette of biochemical parameters. Methods. This prospective, single- center study included 120 patients subjected to elective cholecystectomy during the period of one year. Sixty patients were treated laparoscopically and 60 traditionally. Biohumoral and endocrine parameters were determined from 24-hour urine and blood. We measured adrenaline, noradrenaline, metabolites of corticosteroid hormones (17-hydroxyl and 17-keto steroids), C-reactive protein (CRP), albumin, glycemia, creatine-phosphokinase (CPK), lactate-dehydrogenase (LDH), red blood cells sedimentation and serum concentration of potassium. Results. We observed significantly lower levels of adrenaline (p < 0.01), noradrenaline (p < 0.05), dopamine (p < 0.01), 17-hydroxyl (p < 0.01) and 17-keto steroids (p < 0.01), glycemia (p < 0.01), CPK (p < 0.01), LDH (p < 0.01) and red blood cells sedimentation (p < 0.01) following laparoscopic cholecystectomy compared to traditional one. Significant increase in CRP levels was recorded postoperatively in both groups (p < 0.05), as well as significant decrease in serum albumin values (p < 0.05). Duration of the hospitalization following laparoscopic cholecystectomy was significantly shorter (p < 0.01). Conclusion. The intensity of organism response is proportional to the intensity of surgical trauma. Metabolic, tissue and neuroendocrine response of organism to trauma has lower intensity after laparoscopic cholecystectomy.
Bulajic M.,University of Belgrade |
Bulajic M.,Center for Digestive Endoscopy |
Panic N.,University of Belgrade |
Panic N.,Center for Digestive Endoscopy |
And 6 more authors.
European Journal of Gastroenterology and Hepatology | Year: 2012
Recognition of Helicobacter pylori as an important factor in genesis of gastric adenocarcinoma lead to a large number of studies concerning potential role of Helicobacter spp. in the development of extragastric digestive malignancies. The serological studies indicated possible localizations in the digestive system being from interest in enlightening Helicobacter spp. carcinogenic potential. The PCR obtruded itself as a gold standard in proving existence of actual correlation. In this review, the authors have examined studies conducted in the last 10 years examining Helicobacter spp. correlation with extragastric digestive carcinogenesis. Studies have been observed in four groups referring to hepatic carcinoma, bile duct cancer, pancreatic cancer, and colon cancer. The results of these researches have shown that there is a strong correlation between Helicobacter spp. colonization and primary liver tumors as well as bile duct tumors, whereas conclusions made by authors examining pancreatic cancer are contradictory and demands further investigation. No correlation between Helicobacter spp. and colon cancer have been proven. The PCR subtype most widely used in studies included in this review was nested PCR, whereas genes targeted most frequently for amplification are 16S rDNA of Helicobacter spp. and UreA gene or cagA gene of H. pylori. During the last 10 years PCR has proven itself as a sovereign method for Helicobacter spp. diagnostic in extragastric organs in the digestive system. Knowledge and experiences obtained in this domain could be encouraging for researchers in analogous fields of interest. © 2012 Wolters Kluwer Health | Lippincott Williams & Wilkins.
Li G.-Q.,Center for Digestive Endoscopy |
Zhang Y.-X.,Center for Digestive Endoscopy |
Gonc L.-G.,Center for Digestive Endoscopy |
Yuan X.-X.,Center for Digestive Endoscopy |
And 3 more authors.
Acta Anatomica Sinica | Year: 2015
Objective To study the relationship between cytochrome P450 II E1 (CYP DEI) protein expression of colorectal carcinoma complicated with intestinal schistosomiasis and its possible mechanism. Methods Immunohistochemical SP method was applied to detect the CYP fl E1 protein expression of 60 cases of intestinal schistosomiasis and colorectal cancer tissue, 60 cases of colorectal cancer tissue without intestinal infection of Schistosoma japonicum,60 cases of intestinal schistosomiasis infection group, and 50 cases of normal intestinal tissue without infection of Schistosoma japonicum. Results The rates of the cytochrome p450 II E1 immunohistochemical positive staining were 73.33% in colorectal carcinoma complicated with intestinal schistosomiasis group, 46.67% in the intestinal schitosoma japonicum infection group, 31. 67% in colorectal cancer tissue without intestinal infection of schistosoma japonicum, 16% in the normal intestinal tissues. The cytochrome p450 II E1 immunohistochemical positive in colorectal carcinoma complicated with intestinal schistosomiasis group associated without gender, age, tumor location, lymph node metastasis, but with tissue differentiation degree and pathology classification. The stepwise regression method of logistic multiple factor analysis showed that the histological type was the only risk factors for the positive expression of P450 II E1 ( OR = 11. 4, P = 0. 024). Conclusion CYP H E1 expression is associated with colorectal cancer pathological tissue type. CYP II E1 plays a certain role in the pathogenesis of in colorectal carcinoma complicated with intestinal schistosomiasis.