Time filter

Source Type

Agyei D.,Deakin University | Agyei D.,Center for Chemistry and Biotechnology | Agyei D.,University of Otago | Ahmed I.,Griffith University | And 3 more authors.
Protein and Peptide Letters | Year: 2017

Bioactive proteins and peptides are recognised as novel therapeutic molecules with varying biological properties for potential medical applications. Development of protein and peptidebased therapeutic products for human use is growing steadily as they continue to receive an increasing rate of approval by the United States Food and Drugs Administration (US FDA). In this short review, we describe the current status and methodologies involved in the synthesis of protein and peptide biopharmaceuticals with an emphasis on the drivers and restrains to their exploitation in the therapeutic products sector. © 2017 Bentham Science Publishers.

Agyei D.,Deakin University | Agyei D.,Center for Chemistry and Biotechnology | Ongkudon C.M.,Universiti Malaysia Sabah | Wei C.Y.,Universiti Malaysia Sabah | And 2 more authors.
Food and Bioproducts Processing | Year: 2016

Food protein-derived bioactive peptides (BPs) have been reported to trigger certain physiological responses in the body, thereby influencing health positively. These peptides have attracted high research and consumer interests due to their huge potential of use in functional foods and other dietary interventions of disease control and health promotion. However, successful product development is limited by the fact that current manufacturing processes are either difficult to scale up, high in cost, or have the potential to affect the structure-activity properties of these peptides. To overcome these challenges, we have proposed in this review, the use of an integrated '-omics' approach comprising in silico analysis and '-omics' techniques (such as peptidomics) to respectively forecast and validate the biological and physico-chemical properties of the peptides. This information is then used for the rational design of suitable purification steps for peptides of interest. Downstream purification could also be undertaken by liquid chromatography using monolithic adsorbents physico-chemically engineered (using results of in silico analysis) for rapid isolation of peptides. By coupling the high throughput and predictive capability of '-omics' to the enhanced convective hydrodynamics of monolithic columns, it becomes feasible, even at preparative scale, to produce BPs that meet the requirements of high purity, potency, and cost-effectiveness. © 2016 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

Pravadali S.,University of Western Sydney | Bassanese D.N.,Center for Chemistry and Biotechnology | Conlan X.A.,Center for Chemistry and Biotechnology | Francis P.S.,Center for Chemistry and Biotechnology | And 3 more authors.
Analytica Chimica Acta | Year: 2013

Herein we assess the separation space offered by a liquid chromatography system with an optimised uni-dimensional separation for the determination of the key chemical entities in the highly complex matrix of a tobacco leaf extract. Multiple modes of detection, including UV-visible absorbance, chemiluminescence (acidic potassium permanganate, manganese(IV), and tris(2,2'-bipyridine)ruthenium(III)), mass spectrometry and DPPH radical scavenging were used in an attempt to systematically reduce the data complexity of the sample whilst obtaining a greater degree of molecule-specific information. A large amount of chemical data was obtained, but several limitations in the ability to assign detector responses to particular compounds, even with the aid of complementary detection systems, were observed. Thirty-three compounds were detected via MS on the tobacco extract and 12 out of 32 compounds gave a peak height ratio (PHR) greater than 0.33 on one or more detectors. This paper serves as a case study of these limitations, illustrating why multidimensional chromatography is an important consideration when developing a comprehensive chemical detection system. © 2013 Elsevier B.V.

Loading Center for Chemistry and Biotechnology collaborators
Loading Center for Chemistry and Biotechnology collaborators