Center for Cell Encapsulation Research

Daejeon, South Korea

Center for Cell Encapsulation Research

Daejeon, South Korea
Time filter
Source Type

Kim M.-H.,Center for Cell Encapsulation Research | Park M.,Center for Cell Encapsulation Research | Kang K.,Center for Cell Encapsulation Research | Kang K.,Brain Bio | And 2 more authors.
Biomaterials Science | Year: 2014

Topography, the physical characteristics of an environment, is one of the most prominent stimuli neurons can encounter in the body. Many aspects of neurons and neuronal behavior are affected by the size, shape, and pattern of the physical features of the environment. A recent increase in the use of nanometric topographies, due to improved fabrication techniques, has resulted in new findings on neuronal behavior and development. Factors such as neuron adhesion, neurite alignment, and even the rate of neurite formation have all been highlighted through nanotopographies as complex phenomena that are driven by intricate intracellular mechanisms. Nanotopographies are suitable platforms, not only for fundamental studies on neuronal development, but also in practical applications, including multielectrode array devices and neuro-regenerative medicine. We reviewed recent publications that address the effects of nanotopography on neurons and categorized the observed behaviors as adherence, directional guidance, or accelerated outgrowth. We also discussed possible biological mechanisms of the molecular and cellular responses to topography, and suggested future perspectives for this field. © 2014 The Royal Society of Chemistry.

Park J.H.,Center for Cell Encapsulation Research | Kim J.Y.,Center for Cell Encapsulation Research | Cho W.K.,Chungnam National University | Choi I.S.,Center for Cell Encapsulation Research
Chemistry - An Asian Journal | Year: 2014

Bioinspired silicification attracts a great deal of interest because of its physiologically relevant, mild conditions for hydrolysis and condensation of silica precursors, which makes the bioinspired approach superior to the conventional sol-gel process, particularly when dealing with biological entities. However, the morphological control of silica structures with incorporation of functional groups in the bioinspired silicilication has been unexplored. In this work, we co-silicificated (1 H, 1 H, 2 H, 2 H-perfluorooctyl)triethoxysilane and tetraethyl orthosilicate to investigate the morphological evolution of fluorinated silica structures in the cetyltrimethylammonium bromide-mediated, cysteamine-catalyzed silicification. The generated micrometer-long worm-like and spherical silica structures display superhydrophobicity after film formation. Interestingly, the measurement of dynamic water contact angles shows that the morphological difference leads to a different wetting state, either the self-cleaning or the pinning state of the superhydrophobic surface. Not a can of worms: The co-silicification of tetraethyl orthosilicate and (1 H, 1 H, 2 H, 2H-perfluorooctyl)triethoxysilane generated worm-like or particulate fluorinated silica structures, depending on the water-to-ethanol ratio and the ratio of the two silica precursors. The substrate coated with the fluorinated silica displayed superhydrophobicity, and its surface free energy was much lower than that of Teflon. Copyright © 2014 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Kang K.,Center for Cell Encapsulation Research | Kang K.,Molecular Level Interface Research Center | Joo S.,Korea Advanced Institute of Science and Technology | Choi J.Y.,Center for Cell Encapsulation Research | And 15 more authors.
Proceedings of the National Academy of Sciences of the United States of America | Year: 2015

The posttranslational modification of neural cell-adhesion molecule (NCAM) with polysialic acid (PSA) and the spatiotemporal distribution of PSA-NCAM play an important role in the neuronal development. In this work, we developed a tissue-based strategy for metabolically incorporating an unnatural monosaccharide, peracetylated N-azidoacetyl-D-mannosamine, in the sialic acid biochemical pathway to present N-azidoacetyl sialic acid to PSANCAM. Although significant neurotoxicity was observed in the conventional metabolic labeling that used the dissociated neuron cells, neurotoxicity disappeared in this modified strategy, allowing for investigation of the temporal and spatial distributions of PSA in the primary hippocampal neurons. PSA-NCAM was synthesized and recycled continuously during neuronal development, and the two-color labeling showed that newly synthesized PSANCAMs were transported and inserted mainly to the growing neurites and not significantly to the cell body. This report suggests a reliable and cytocompatible method for in vitro analysis of glycans complementary to the conventional cell-based metabolic labeling for chemical glycobiology.

Yang S.H.,Korea National University of Education | Hong D.,Center for Cell Encapsulation Research | Lee J.,Center for Cell Encapsulation Research | Ko E.H.,Center for Cell Encapsulation Research | Choi I.S.,Center for Cell Encapsulation Research
Small | Year: 2013

Cells are encapsulated individually within thin and tough shells in a cytocompatible way, by mimicking the structure of bacterial endospores that survive under hostile conditions. The 3D 'cell-in-shell' structures - coined as 'artificial spores' - enable modulation and control over cellular metabolism, such as control of cell division, resistance to external stresses, and surface-functionalizability, providing a useful platform for applications, including cell-based sensors, cell therapy, regenerative medicine, as well as for fundamental studies on cellular metabolism at the single-cell level and cell-to-cell communications. This Concept focuses on chemical approaches to single-cell encapsulation with artificial shells for creating artificial spores, including cross-linked layer-by-layer assembly, bioinspired mineralization, and mussel-inspired polymerization. The current status and future prospects of this emerging field are also discussed. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.

Loading Center for Cell Encapsulation Research collaborators
Loading Center for Cell Encapsulation Research collaborators