Center for Biological Science

Kumaracoil, India

Center for Biological Science

Kumaracoil, India

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Saravanan G.,Center for Biological Science | Ponmurugan P.,KS Rangasamy College of Technology
Journal of Trace Elements in Medicine and Biology | Year: 2013

It is suggested that iron may play a role in the pathogenesis of diabetes. Iron is not only chaperoned through its essential functional pathways, but it also causes damage to biological systems by catalyzing the production of reactive oxygen species. So, the parenchymal tissues of several organs are subject to cell injury and functional insufficiency due to excess deposition of iron. The present study investigated the effects of S-allylcysteine (SAC), a sulphur containing amino acid derived from garlic on the changes in iron metabolism induced by oxidative stress in tissues, as well as on serum biochemical parameters of streptozotocin (STZ)-induced diabetic rats. SAC was administered orally for 45. days to control and experimental diabetic rats. The effects of SAC on glucose, insulin, serum iron, ferritin, transferrin, serum bilirubin, heart heme oxygenase activity (HO) and δ-aminolevulinicacid dehydratase activity (δ-ALA-D) in liver and kidneys were studied. The levels of glucose, iron, ferritin, bilirubin and HO in liver were increased significantly (p<0.05) whereas the levels of insulin, transferrin and δ-ALA-D in tissues were decreased in diabetic rats. Administration of SAC to diabetic rats showed a decrease in blood glucose, iron, ferritin, bilirubin and HO. In addition, the levels of insulin, transferrin and δ-ALA-D activity in tissues were increased in SAC treated diabetic rats. These findings suggest that S-allylcysteine could have a protective effect against alterations in oxidative stress induced iron metabolism in the diabetic state which was evidenced by the capacity of this natural antioxidant to modulate parameters of iron metabolism. © 2012 Elsevier GmbH.


Cardew A.S.,Center for Biological science | Brown T.,University of Southampton | Fox K.R.,Center for Biological science
Nucleic Acids Research | Year: 2012

In order to enhance DNA triple helix stability synthetic oligonucleotides have been developed that bear amino groups on the sugar or base. One of the most effective of these is bis-amino-U (B), which possesses 5-propargylamino and 2′-aminoethoxy modifications. Inclusion of this modified nucleotide not only greatly enhances triplex stability, but also increases the affinity for related sequences. We have used a restriction enzyme protection, selection and amplification assay (REPSA) to isolate sequences that are bound by the heavily modified 9-mer triplex-forming oligonucleotide B6CBT. The isolated sequences contain An tracts (n=6), suggesting that the 5′-end of this TFO was responsible for successful triplex formation. DNase I footprinting with these sequences confirmed triple helix formation at these secondary targets and demonstrated no interaction with similar oligonucleotides containing T or 5-propargylamino-dU. © 2011 The Author(s).


Saravanan G.,Center for Biological Science | Ponmurugan P.,KS Rangasamy College of Technology
Journal of the Science of Food and Agriculture | Year: 2012

BACKGROUND: Glycoprotein is one of the components of the cardiac extracellular matrix and plays an important role in cardiac remodelling during various cardiac diseases, including myocardial infarction (MI). This study was aimed at evaluating the preventive role of Amaranthus viridis Linn. on C-reactive protein (CRP), total protein, albumin, globulin, ceruloplasmin and glycoproteins in the serum and heart of experimental induced myocardial infarcted (MI) rats. RESULTS: MI was induced in male Wistar rats by subcutaneous injection of 20 mg kg-1 isoproterenol (ISO) kg-1 body weight (BW) twice at an interval of 24 h. ISO-induced MI rats showed a significant increase in the levels of serum CRP and ceruloplasmin and a significant decrease in the levels of serum total protein, albumin and globulin. Glycoprotein levels in the serum and heart were increased in ISO-induced MI rats. Oral administration of 300 mg A. viridis kg-1 BW day-1 for a period of 45 days altered the metabolic derangement in ISO-induced MI rats. CONCLUSION: This study exemplifies the protective effect of A. viridis on ISO-induced cardiotoxicity in male Wistar rats. The data further reinforce the cardioprotective effect of A. viridis by altering CRP and glycoprotein levels. © 2012 Society of Chemical Industry.


Arunagiri K.,Bharathiar University | Sivakumar T.,Bharathiar University | Murugan T.,Center for Biological Science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The intention of the present study was to investigate the prevalence of Vibrio species in seafood samples. Methods: A total of 20 seafood samples include finfishes and crustaceans of marine origin were collected from the local fish market of Kanchipuram, Tamil Nadu. Vibrio colonies were isolated from the samples and identified by cultural, morphological, and biochemical studies. Then, the percentage frequencies of the Vibrio species were intended. Results: A total of 58 Vibrio colonies were isolated from the collected seafood samples. In this, Barracuda fishes contained a higher number of Vibrio colonies (16.07%) followed by Indian mackerel and Crustaceans (12.5%). The foremost three Vibrio species, viz., Vibrio cholerae, Vibrio Parahaemolyticus, and Vibrio alginolyticus were identified by studying cultural, morphological, and biochemical characteristics, and the incidence percentage was found as 22.41%, 17.24%, and 12.07%, respectively, from the total Vibrio colonies isolated. Conclusion: Thus, these results revealed that the pathogenic Vibrio species of V. cholerae and V. parahaemolyticus was found higher percentages in collected seafoods. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.


Saravanan G.,Center for Biological Science | Ponmurugan P.,KS Rangasamy College of Technology
Food Bioscience | Year: 2013

The present study was designed to investigate the effect of the administration of S-allylcysteine (SAC) (150 mg/kg body weight for 45 days) on acetylcholinesterase (AChE) activity and antioxidant levels in the brain tissues of streptozotocin-induced diabetic rats. The levels of glucose, TBARS, hydroperoxide and acetylcholinesterase were increased significantly whereas the levels of plasma insulin, reduced glutathione, superoxide dismutase and catalase were decreased in experimental diabetic rats. Administration of SAC to diabetic rats reverted all these parameters. The effect of SAC was compared with glyclazide, a well-known antioxidant and antihyperglycemic drug. In conclusion, the present findings showed that treatment with SAC prevents the increase in AChE activity, lipid peroxidation, and consequently improves the antioxidant system in diabetic rats, indicating that this compound can be considered as possible therapeutics to be investigated in brain disorders associated with the diabetes. © 2013 Elsevier Ltd.


Saravanan G.,Center for Biological science | Ponmurugan P.,KS Rangasamy College of Technology
International Journal of Endocrinology and Metabolism | Year: 2013

Background: S-allylcysteine, a garlic derivative, could have a protective effect against pathogenesis of diabetes mellitus. Objectives: Sustained free radical generation and oxidative damage to system leads to the final conclusion phase of diabetes and also it coexists with a constant diminution in the antioxidant status.The present study aims to evaluate the therapeutic effects of S-allylcysteine (SAC) against adipocytes hormones and antioxidant defense systems of plasma and erythrocytes of treptozotocin (STZ) induced diabetes in rats. Materials and Methods: Diabetic rats were administered SAC (150 mg/kg b.w) orally for 45 days. At 46th day, the rats were anesthetized, and blood and liver sample were collected for analyzing glucose, plasma insulin, CYP2E1 activity, Thiobarbituric acid reactive substances (TBARS), hydroperoxide, enzymatic and nonenzymatic antioxidants, reduced glutathione (GSH), ceruloplasmin, plasma leptin, and adiponectin. Results: The levels of glucose, CYP2E1 activity, Thiobarbituric acid reactive substances (TBARS), hydroperoxide, and ceruloplasmin were increased significantly; whereas, the levels of plasma insulin, reduced glutathione, enzymatic and nonenzymatic antioxidants, leptin and adiponectin were decreased in experimental diabetic rats. Administration of SAC to diabetic rats led to a decrease in the levels of glucose, CYP2E1 activity, TBARS, and ceruloplasmin. In addition, the levels of plasma insulin, enzymatic and nonenzymatic antioxidants leptin and adiponectin were increased in SAC treated diabetic rats. Gliclazide, a standard drug for diabetes, was used for the comparative purpose. Conclusions: The results of the present investigation suggest that SAC could be used as a food supplement in the treatment of diabetes characterized by provoked antioxidant status, altered blood glucose, and hormones level.


Saravanan G.,Center for Biological Science | Ponmurugan P.,KS Rangasamy College of Technology
Journal of Diabetes and its Complications | Year: 2012

Objective: It is considered that diabetes mellitus and thyroid disease are the two common endocrine disorders and also suggested that insulin and thyroid hormones influence each other actions. The present study was designed to investigate the effect of the administration of S-allylcysteine (SAC), a sulfur containing amino acid derived from garlic on blood glucose, insulin, HbA1C, total protein, albumin, Thyroid hormone (T3, T4), TSH, TBARS and circulatory antioxidant levels (SOD, CAT, GSH and GPx) in STZ-induced diabetic rats. Methods: SAC was administered orally for 45 days to control and STZ induced diabetic rats. The effects of SAC on glucose, plasma insulin, HbA1C, total protein, albumin, Thyroid hormone, TSH and circulatory antioxidant levels were studied. Results: The levels of glucose, TBARS, hydroperoxide and HbA1C were increased significantly whereas the levels of plasma insulin, reduced glutathione, superoxide dismutase, catalase, GSH, GPx, total protein, albumin, Thyroid hormone and TSH were decreased in STZ induced diabetic rats. Administration of SAC to diabetic rats showed a decrease in plasma glucose, TBARS, hydroperoxide and HbA1C. In addition, the levels of plasma insulin, SOD, CAT, GPx, GSH, total protein, albumin, Thyroid hormone and TSH were increased in SAC treated diabetic rats. The effect of SAC was compared with gliclazide, a well-known antioxidant and antihyperglycemic drug. Conclusion: From these findings, it is indicated that SAC might be acting through activation in the synthesis and/or secretion of circulating thyroid hormones which in turn stimulate the synthesis of insulin. © 2012 Elsevier Inc. All rights reserved.


Saravanan G.,Center for Biological Science | Ponmurugan P.,KS Rangasamy College of Technology | Deepa M.A.,Kristu Jayanthi College | Senthilkumar B.,C Abdul Hakeem College | Senthilkumar B.,Tamil University
Journal of the Science of Food and Agriculture | Year: 2014

Background: Obesity represents a rapidly growing threat to the health of populations and diet intervention has been proposed as one of the strategies for weight loss. Ginger and its constituents have been used for their anti-flatulent, expectorant and appetising properties and they are reported to possess gastro-protective and cholesterol-lowering properties. The present study investigated the effects of gingerol on the changes in body weight, serum glucose, insulin, insulin resistance and lipid profile in plasma and liver as well as on the activity of amylase, lipase and leptin in high-fat diet (HFD)-induced obese rats. Results: HFD-induced obese rats were treated orally with gingerol (25, 50 and 75 mg kg-1) once daily for 30 days. A lorcaserin-treated group(10 mg kg-1)was included for comparison. The levels of bodyweight, glucose, lipid profile and insulin, insulin resistance, leptin, amylase and lipase were increased significantly (P < 0.05) in HFD rats. Rats treated with gingerol and fed a HFD showed significantly (P<0.05) decreased glucose level, bodyweight, leptin, insulin, amylase, lipase plasma and tissue lipids when compared to normal control. The effect at a dose of 75 mg kg-1 of gingerol was more pronounced than that of the dose 25 mg kg-1 and 50mg kg-1. The lorcaserin-treated group also manifested similar effects to those of gingerol. Conclusion: These findings suggested that ginger supplementation suppresses obesity induced by a high fat diet and itmight be a promising adjuvant therapy for the treatment of obesity and its complications. © 2014 Society of Chemical Industry.


Beula Rani K.R.,Alagappa Chettiar College of Engineering And Technology | Sundar S.K.,Mr Government Arts College | Murugan M.,Center for Biological Science
Asian Journal of Pharmaceutical and Clinical Research | Year: 2016

Objective: The study was undertaken to evaluate the antimicrobial activity and phytochemical constituents of the medicinal plant Alpinia galanga. Methods: The plant samples were collected from the places of Mandaikadu, Kanyakumari District, Tamil Nadu, India. Plant extracts were prepared from the dried leaf powder using different solvents include acetone, chloroform, diethyl ether, ethanol, and distilled water. Initially, antimicrobial activities of the plant extracts were tested by agar well diffusion method against four bacterial, viz., Escherichia coli, Klebsiella pneumonia, Bacillus cereus, and Staphylococcus aureus and two fungal pathogens, viz., Aspergillus niger and Penicillium chrysogenum. Further, the plant extracts were screened for phytochemical constituents by standard protocols. Results: In this study, the entire bacterial pathogens have inhibited by acetone, chloroform, diethyl ether, and ethanol extracts. In phytochemical screening tests, the plant extracts showed positive results for carbohydrates, amino acid, chloride, alkaloids, flavonoids, phenols, and terpenoids. Conclusion: This study reveals the continuation of antimicrobial components in the medicinal plant A. galanga. © 2016, Innovare Academics Sciences Pvt. Ltd. All rights reserved.


Murugan K.,Center for Biological science | Murugan K.,King Saud University | Al-Sohaibani S.A.,King Saud University
Research Journal of Microbiology | Year: 2010

Tannins, the important recalcitrant natural polymers released along with tamely effluents limits their treatment and reuse. The currently employed treatment methods failed to degrade them completely. An attempt has been made specifically to treat tannin and remove associated color from the tannery effluent. The biomass of mango industry solid waste isolate Aspergillus candidus MTTC 9628 and the tannase enzyme from this organisms were found to degrade and thereby reduce the tannin content of the tannery effluent resulting in the decolourisation of the effluent. The results obtained after treatment with the immobilized tannase enzyme also showed considerable reduction in effluent tannin content and hence the color of the effluent. However, it is observed that the whole organisms treatment have a fundamental advantage over enzymatic treatment as they transform a broad range of compounds resulting decrease in Biological Oxygen Demand (BOD), Chemical Oxygen Demand (COD) and other physicochemical characteristic values. This present works reveals that these undegradable tannins can be specifically removed by enzymatic treatment. Development of suitable treatment facility employing tannase enzymes could be adapted at laboratory and industrial level treatment thereby making it suitable for further use. © 2010 Academic Journals Inc.

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