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Saito N.,Meiji Pharmaceutical University | Suwanborirux K.,Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi | Chanvorachote P.,Chulalongkorn University | Chanvorachote P.,Bangkok University
Anticancer Research | Year: 2013

Background: The strategies for achieving antimetastasis have received increased research interest and clinical attention. The anoikis-sensitizing effect of ecteinascidin 770 (ET-770) was investigated in the present study in non-small cell lung cancer cells. Materials and Methods: ET-770 isolated from Ecteinascidia thurstoni was tested for its anoikissensitizing effect on H23 and H460 human lung cancer cells by 2,3-b-(2-methoxy-4-nitro-5-sulfophenyl)-2H- tetrazolium-5- carboxanilide salt (XTT) assay. The levels of proteins being involved in anoikis of cells were determined by western blot analysis. Results: ET-770 was shown to enhance anoikis response of human lung cancer H23 cells in a dose-dependent manner. The underlying mechanism was investigated and it was found that ET-770 sensitized the cells by activating the p53 protein, which in turn down-regulated anti-apoptotic myeloid cell leukemia sequence-1 (MCL1) and up-regulated BCL2-associated X protein (BAX) proteins. However, B-cell lymphoma-2 (BCL2) proteins were not significantly affected by ET-770. Further, the anoikis sensitization of ET-770 was observed in H460 lung cancer cells. Conclusion: The present results reveal for the first time that ET-770 can sensitize anoikis through the p53 pathway and further development of this compound for therapeutic use is warranted. © 2013 Anticancer Research.


Thawai C.,King Mongkut's University of Technology Thonburi | Thawai C.,Microbial Resource Management Unit | Tanasupawat S.,Chulalongkorn University | Suwanborirux K.,Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi | And 2 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2011

Two actinomycete strains, CM9-9T and AK2-48, which produced straight rod-shaped, non-motile cells, were isolated from soils in Chiang Mai and Phuket provinces, respectively, Thailand. The morphological and chemotaxonomic characteristics of the isolates coincided with those of the genus Agromyces. Phylogenetic analysis using 16S rRNA gene sequences also indicated that the isolates were clearly separated from their closest relative, Agromyces aurantiacus YIM 21741T, and should be classified in the genus Agromyces. Furthermore, a combination of DNA - DNA hybridization results and physiological and biochemical properties indicated that the isolates could be distinguished from all recognized members of the genus Agromyces. The isolates therefore represent a novel species, for which the name Agromyces tropicus sp. nov. is proposed. The type strain is CM9-9T (=JCM 15672T =BCC 34764T). © 2011 IUMS Printed in Great Britain.


Supong K.,King Mongkut's University of Technology Thonburi | Suriyachadkun C.,National Science and Technology Development Agency | Tanasupawat S.,Chulalongkorn University | Suwanborirux K.,Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi | And 4 more authors.
International Journal of Systematic and Evolutionary Microbiology | Year: 2013

An actinomycete strain, designated strain SH2-13T, was isolated from a marine sediment sample collected from the Andaman Sea of Thailand. Applying a polyphasic approach, the isolate was identified as a member of the genus Micromonospora using morphological and chemotaxonomic characteristics, including the presence of meso-diaminopimelic acid in the peptidoglycan. Whole-cell sugars were arabinose, galactose, glucose, rhamnose, ribose and xylose. Diagnostic polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol, phosphatidylinositol mannosides and phosphoglycolipids. The major menaquinones were MK-10(H2), MK-10(H4) and MK-10(H6). 16S rRNA gene sequence analysis revealed similarity to Micromonospora marina JSM1-1T (99.1%), Micromonospora coxensis 2-30-b(28)T (99.1%), Micromonospora aurantiaca DSM 43813T (98.8%) and Micromonospora chalcea DSM 43026T (98.7%). However, a combination of DNA-DNA hybridization results and phenotypic properties indicated that strain SH2-13T (=NBRC 107934T=BCC 45601T) should be classified as the type strain of a novel species, with the proposed name Micromonospora sediminicola sp. nov. © 2013 IUMS.


Thawai C.,King Mongkut's University of Technology Thonburi | Tanasupawat S.,Chulalongkorn University | Suwanborirux K.,Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi | Suwanborirux K.,Chulalongkorn University | Kudo T.,RIKEN
International Journal of Systematic and Evolutionary Microbiology | Year: 2010

Two actinomycete strains, CM2-8T and CM2-12, were isolated from temperate peat swamp forest soil in Chiang Mai Province, Thailand. Their taxonomic positions were determined using a polyphasic approach. Chemotaxonomic characteristics of these strains coincided with those of the family Micromonosporaceae, i.e. cell wall chemotype II, N-glycolyl type of muramic acid, and type II phospholipids. Phylogenetic analysis based on 16S rRNA gene sequence data also indicated that these strains fell within the family Micromonosporaceae and formed a distinct taxon in the Micromonosporaceae phylogenetic tree. On the basis of phylogenetic analysis, characteristic patterns of 16S rRNA gene signature nucleotides and chemotaxonomic data, it is proposed that the novel isolates belong to a new genus, Actinaurispora gen. nov. The type species of the genus is proposed as Actinaurispora siamensis sp. nov., with strain CM2-8T (=JCM 15677T=BCC 34762T) as the type strain. © 2010 IUMS.


Tsujimoto M.,Meiji Pharmaceutical University | Lowtangkitcharoen W.,Center for Bioactive Natural Products from Marine Organisms and Endophytic Fungi | Mori N.,Meiji Pharmaceutical University | Pangkruang W.,Khon Kaen University | And 3 more authors.
Chemical and Pharmaceutical Bulletin | Year: 2013

We report herein eleven 2'-N-acyl derivatives that were prepared from ecteinascidin 770 (Et 770: 1b) via 18,6'-O-bisallyl protected compound (4) in excellent yields. 2'-N-Acyl derivatives (6a-k) generally showed higher cytotoxicity than 1b. Among them, 3-quinolineacyl derivative (6g) and 4-fluorocinnamoyl derivative (6h) exhibited approximately 50- and 70-fold higher cytotoxicity to the HCT116 human colon carcinoma cell line, respectively, than 1b. Both compounds are potent inhibitors of the in vitro growth of several tumor cells and are therefore promising leads for further optimization. We also report the transformation of 1b into Et 788 (3), which is the first example of an ecteinascidin derivative having a primary amide at C-21 position. © 2013 The Pharmaceutical Society of Japan.

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