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Dehradun, India

Mohan M.,P.A. College | Haider S.Z.,Center for Aromatic Plants | Sharma A.,Indian Institute of Integrative Medicine | Seth R.,P.A. College
Asian Pacific Journal of Tropical Disease | Year: 2012

Objective: To examine the composition of Cinnamomum tamala and Murraya koenigii essential oils and their antimicrobial activities against nine microbial strains. Methods: Essential oils were obtained by hydrodistillation from the leaves of two spice trees and were analyzed by GC and GC/MS. The oils were also tested for their antimicrobial activity using broth micro dilution method. Results: Cinnamaldehyde (37.85%) and cis-linalool oxide (29.99%) were the main components characterized in the oil of C. tamala, whereas α-pinene (39.93%), sabinene (13.31%) and trans-caryophyllene (9.02%) detected as the major constituents in M. koenigii oil. C. tamala oil exhibited significant antifungal activity and satisfactory antibacterial activity, while lesser antimicrobial activity was observed in M. koenigii oil. Conclusions: The present study suggested that C. tamala oil was more effective against bacterial and fungal strains as compared with M. koenghii oil. © 2012 Asian Pacific Tropical Medicine Press. Source

Mohan M.,P.A. College | Seth R.,P.A. College | Singh P.,P.A. College | Lohani H.,Center for Aromatic Plants | Gupta S.,SBSPGI
National Academy Science Letters | Year: 2012

The volatiles constituents obtained by hydro-distillation of all the aerial parts of two Lamiaceae plants were analyzed byGCandGC/MS. The Hyssopus officinalis (L.) was collected from Malari (Chamoli) while the Thymus serpyllum (L.) collected from three places Malari; Indradhara near Badrinath (Chamoli) and Chakrata (Dehradun) of the Garhwal region of Uttarakhand. H. officinalis essential oil identification showed 22 constituents representing 96.66 %of the oil, having monoterpene hydrocarbons (22.10 %), oxygenated monoterpene (74.24 %), one sesquiterpene hydrocarbon (0.18 %) and one oxygenated sesquiterpene hydrocarbon (0.14 %),while the extracted T. serpyllum essential oils led to the identification of 28 constituents representing 99.84, 97.13 and 97.11 %, respectively, having monoterpene hydrocarbons (10.11- 19.7 %), oxygenated monoterpenoids (68.78-83.82 %), sesquiterpene hydrocarbons (5.74-7.60 %) and oxygenated sesquiterpene hydrocarbons (0.17-1.02 %). The major volatiles constituents of these essential oils were oxygenated monoterpenoids. Pinocarvone (32.14 %) and iso-Pinocamphone (30.70 %) identified as major volatiles constituents in H. officinalis. However, thymol (37.27-55.56 %) followed by thymyl methyl ether (3.26-12.93 %) were the major components presented in T. serpyllum. © The National Academy of Sciences, India 2012. Source

Haider S.Z.,Center for Aromatic Plants | Mohan M.,DAV PG College | Pandey A.K.,Gorakhpur University | Pandey A.K.,National Institute of Plant Health Management Ministry of Agriculture | Singh P.,Gorakhpur University
Journal of Oleo Science | Year: 2015

The repellent and fumigant toxicity of essential oils of Tanacetum nubigenum Wallich. ex DC collected from three different habitats (Gothing, Burphu and Glacier) of Uttarakhand Himalayas, India named as TNG, TNB and TNM respectively, were investigated against the adults of red flour beetle Tribolium castaneum (Herbst). Among the three samples tested, TNG was found to more potent exhibiting more repellent effect towards the insects and with LC50 values by fumigant bioassay were 13.23 and 8.32 μl per 0.25 L air at 24 and 48 h exposure of insects to the essential oil respectively. In between other two oil samples, TNM was superior in potency showed LC50 value of 14.22 (24 h) & 8.82 μl per 0.25 L air (48 h). During in vivo study all the essential oil samples significantly protected 500 g of wheat grains for 6 months from insect infestation as compared to non fumigated grains and order of efficacy was TNG>TNM>TNB. There were no side effects of essential oils on germination rate of grains (<85%) exposed for 6 months after fumigation. The present study suggests that essential oil of T. nubigenum can be explored as novel natural fumigants for the control of stored product insects. © 2015 by Japan Oil Chemists’ Society. Source

Chauhan N.K.,Center for Aromatic Plants | Singh S.,Center for Aromatic Plants | Haider S.Z.,Center for Aromatic Plants | Lohani H.,Center for Aromatic Plants | Kushwaha B.L.,P.A. College
National Academy Science Letters | Year: 2012

Perilla frutescens L., known as ''Bhanjira'' is a high value plant used in flavoring foods by local villagers of Uttarakhand. The seeds were sown at 25 days intervals in the month of May, June and July. The samples of whole plant parts were collected at pre and full flowering stages and hydro-distilled using a Clevenger type apparatus. The essential oils were analyzed by GC/MS. In all the oils, perillaketone (43.49-75.46%) and 1-methyl-2-methylene trans-decalin (13.17-29.22%) were found to be the most abundant components. Although, the qualitative essential oil compositions were found similar in different sowing times and growth stages, but some quantitative variations were occurred. © 2012 The National Academy of Sciences India. Source

Mohan M.,Center for Aromatic Plants | Haider S.Z.,Center for Aromatic Plants | Andola H.C.,Center for Aromatic Plants | Purohit V.K.,Hemwati Nandan Bahuguna Garhwal University
Research Journal of Pharmaceutical, Biological and Chemical Sciences | Year: 2011

The environmental problems caused by overuse of pesticides have been the matter of concern for both scientists and public in recent years. It has been estimated that about 2.5 million tons of pesticides are used on crops each year and the worldwide damage caused by pesticides reaches $100 billion annually. Natural products are an excellent alternative to synthetic pesticides as a means to reduce negative impacts to human health and the environment. The move towards green chemistry processes and the continuing need for developing new crop protection tools with novel modes of action makes discovery and commercialization of natural products as green pesticides are good alternatives to chemical pesticides. Green pesticides are eco-friendly, economic, target-specific and biodegradable. Many plant essential oils show a broad spectrum of activity against pest insects and plant pathogenic fungi ranging from insecticidal, antifeedant, repellent, oviposition deterrent, growth regulatory and antivector activities. This special regulatory status combined with the wide availability of essential oils from the flavor and fragrance industries, has made it possible to fast track commercialization of essential oil-based pesticides. Though well received by consumers for use against home and garden pests, these "green pesticides" can also prove effective in agricultural situations, particularly for organic food production. Further, while resistance development continues to be an issue for many synthetic pesticides, it is likely that resistance will develop more slowly to essential oil based pesticides owing to the complex mixtures of constituents that characterize pesticides based on plant essential oils or their constituents have demonstrated efficacy against a range of stored product pests, domestic pests, blood feeding pests etc. These features indicate that pesticides based on plant essential oils could be used in a variety of ways to control a large number of pests. Some essential oil constituents, for example, limonene, pulegone, citronellal and 1,8-cineole are active ingredients of commercially available flea shampoos, mosquito repellents and different agrochemicals. Source

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