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Pradhan P.,Center for Applied Microbiology and Biotechnology | Mohanty R.C.,Utkal University | Mishra A.,Center for Applied Microbiology and Biotechnology
International Journal of Probiotics and Prebiotics | Year: 2011

The aim of this research was to evaluate some probiotic traits of Lactobacillus strains isolated from different sources (dairy foods, vagina, stool and common probiotic drugs) and also to investigate the antibacterial properties of these strains against the urogenital pathogens isolated from urine and vaginal swabs of pregnant women. For this purpose 13. Lactobacillus strains were screened for their probiotic traits and tested against the multi-drug resistant urogenital pathogens. In the present study 2 strains of E. coli, 2 strains of Staphylococcus aureus (MRSA) and one strain of Streptococcus agalactiae were found to be resistant to all the antibiotics tested 5 Lactobacillus strains (45.4%), L. fermentum, L. plantarum, L. acidophilus, L. rhamnosus ahd L. gasseri were found to have good probiotic traits and showed broad antibacterial spectrum against E. coli and S. aureus. All the selected Lactobacilli were observed to produce lactic acid, H2O2 and bacteriocin. Streptococcus agalactiae was inhibited by only L. plantarum (21±6.16 mm). Six Lactobacillus strains were effective against E. coli and eight Lactobacillus strains were found to be effective against S. aureus respectively. Copyright © 2011 by New Century Health Publishers, LLC.

Popova A.V.,Center for Applied Microbiology and Biotechnology | Zhilenkov E.L.,Center for Applied Microbiology and Biotechnology | Myakinina V.P.,Center for Applied Microbiology and Biotechnology | Krasilnikova V.M.,Center for Applied Microbiology and Biotechnology | Volozhantsev N.V.,Center for Applied Microbiology and Biotechnology
FEMS Microbiology Letters | Year: 2012

Acinetobacter baumannii plays a significant role in infecting patients admitted to hospitals. Many A. baumannii infections, including ventilation-associated pneumonia, wound, and bloodstream infections, are common for intensive care and burn units. The ability of the microorganism to acquire resistance to many antibiotics, disinfectants, and dehydration assures its long-term survival in hospital settings. The application of bacteriophages is a potential tool to control A. baumannii infections. Bacteriophage AP22 lytic for A. baumannii was isolated from clinical materials and classified as a member of the Myoviridae family. The phage had an icosahedral head of 64 nm in diameter and a contractile tail of 85-90 nm in length. According to restriction analysis, AP22 had 46-kb double-stranded DNA genome. The phage AP22 exhibited rapid adsorption (> 99% adsorbed in 5 min), a large burst size (240 PFU per cell), and stability to the wide range of pH. The bacteriophage was shown to specifically infect and lyse 68% (89 of 130) genotype-varying multidrug-resistant clinical A. baumannii strains by forming clear zones. Thus, it could be used as a candidate for making up phage cocktails to control A. baumannii-associated nosocomial infections. © 2012 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.

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