Prakash C.,Center for Animal Disease Research and Diagnosis |
Das P.,IVRI |
Sunil Kumar B.V.,GADVASU |
Singh V.,National Research Center on Mithun |
And 2 more authors.
National Academy Science Letters | Year: 2015
Present study evaluated nucleotide sequence variability in 3′ end of insertion sequence IS407A in B. mallei NCTC 3709 strain and compared with other B. mallei strains reported worldwide. This insertion sequence IS407A was found conserved among all B. mallei strains studied while it was found absent in B. pseudomallei. Flagellar gene (motility gene) showed single nucleotide polymorphism in B. mallei strains. This insertion sequence can be used as a molecular signature for B. mallei organism and it can be successfully used in development of multiplex PCR for simultaneous detection of multiple equine pathogens like B. mallei (glanders), B. pseudomallei (Melioidosis) and Streptococcus equi (strangles). © 2014, The National Academy of Sciences, India.
Telang A.,Center for Animal Disease Research and Diagnosis
Environmental Toxicology | Year: 2015
This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p<0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p<0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p<0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p<0.05) in IM-treated rats. IM exposure resulted in significant increase (p<0.05) in LPO and decrease (p<0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation. © 2015 Wiley Periodicals, Inc.
Pandey A.B.,Indian Veterinary Research Institute |
Nandi S.,Center for Animal Disease Research and Diagnosis |
Tiwari A.K.,Indian Veterinary Research Institute |
Audarya S.D.,Veterinary Science University of Madhya Pradesh |
And 3 more authors.
OIE Revue Scientifique et Technique | Year: 2014
Infectious pustular balanoposthitis (IPB) is one of the reproductive disorders caused by bovine herpesvirus 1 (BoHVI) that can be transmitted through artificial insemination. A herd of 63 breeding bulls at a frozen semen bank in Odisha state in India experienced a suspected outbreak of IPB, with 11 bulls showing clinical signs of the infection. Clinical signs were noticed in two bulls initially and a few days after in the other nine animals. Serum samples from 53 bulls were examined for anti-BoHV1 antibodies using a virus neutralisation test (VNT) and a competitive enzyme-linked immunosorbent assay (cELISA); the remaining ten bulls were not included in the study because it was difficult to restrain them at that time. Paired serum samples were collected 21 days apart from ten clinically affected bulls (the eleventh clinically affected bull was not included in the study for the reason stated above). In the neutralisation test, the paired serum samples showed a two- To fourfold increase in anti-BoHV1 antibody titre; in the cELISA, the paired samples were also found positive for anti-BoHV1 antibodies. Serum samples from 43 in-contact bulls were collected about day 22 after the first observation of clinical infection in the herd. Among these serum samples, a total of 30 were found positive for anti-BoHV1 antibodies in the VNT and a total of 30 were found positive in cELISA. Ten samples were positive in one test but not the other and 25 tested positive in both tests. In all, 35 serum samples from in-contact bulls tested positive in either one or both of the two types of test. An overall agreement of 76.74% was found in detection of anti-BoHV1 antibodies in the two tests. Sensitivity was higher than specificity in detection of anti-BoHV1 antibodies in the serum samples. The glycoprotein C region of the genomic DNA of BoHVI was amplified from semen samples by polymerase chain reaction. The findings from the outbreak indicate that continuous monitoring of breeding bulls at frozen semen banks is warranted to avoid the risks associated with artificial insemination.
Ramane S.,Mycobacteria Laboratory |
Verma R.,Center for Animal Disease Research and Diagnosis |
Mondal T.,Mycobacteria Laboratory |
Upmanyu V.,Indian Veterinary Research Institute
Journal of Pure and Applied Microbiology | Year: 2014
Mycobacterium bovis 3/86 strain isolated from cattle was characterized based on RD region encoded Mb3904, Mb3905 and Mb2002c gene sequences. PCR was performed to amplify Mb3904, Mb390S and Mb2002c genes. Restriction enzymes digested amplified geneswere cloned in compatible pET vector and sequenced with vector specific primers. The sequenced genes and its deduced amino acid sequences were compared with the published sequences of reference strains. The sequences of the Mb3904, Mb3905 and Mb2002c genes share 99.6 to 100% nucleotide homology and 99.5 to 100% deduced protein sequence homology for all studied genes with published reference mycobacterial strains indicating their conserved nature.
Dolker S.,Indian Veterinary Research Institute |
Verma R.,Indian Veterinary Research Institute |
Prakash C.,Indian Veterinary Research Institute |
Shamal A.,Indian Veterinary Research Institute |
Shamal A.,Center for Animal Disease Research and Diagnosis
Indian Journal of Animal Sciences | Year: 2012
Diagnostic potentiality of loop mediated isothermal amplification (LAMP) assay with human sputum samples was compared with smear microscopy, culture and PCR in providing rapid and accurate TB diagnosis. Practical application of this assay was emphasized as a supplementary diagnostic test with acid fast smear microscopy for controlling tuberculosis in limited resource setting particularly India. Sputum samples (261) from TB suspected patients were collected, processed and subjected to smear microscopy, culture, PCR and LAMP assay. These 4 diagnostic methodologies were compared in terms of sensitivity, specificity, and rapidity and user friendliness. Sensitivity of LAMP, PCR and microscopy were 97.47, 97.47, and 61.61%, respectively, and specificity of LAMP, PCR and microscopy were 60.31, 74.60 and 100% respectively. Higher sensitivity of diagnostic test will be more useful in high TB endemicity area to cover most of TB patients under National Tuberculosis Control Programmae. This LAMP assay may be a viable alternative of costly PCR diagnosis. This test may be adopted as supplementary diagnostic test with acid fast smear microscopy for routine diagnosis of human TB.
Khan F.A.,University of Wisconsin - Madison |
Das G.K.,University of Wisconsin - Madison |
Pande M.,University of Wisconsin - Madison |
Singh R.,Center for Animal Disease Research and Diagnosis |
Ghosh S.K.,Indian Veterinary Research Institute
Buffalo Bulletin | Year: 2013
Histological examination of H&E stained sections of ovaries collected from cyclic and acyclicbuffaloes (n=6/group) was done in order to evaluate the degree of follicular atresia. The percentages of healthy and atretic follicles were different (P<0.05) between cyclic (53.1% and 46.9%, respectively) and acyclic (10.7% and 89.3%, respectively) buffaloes. Electrophoretic patterns of follicular fluid proteins, studied by SDS-PAGE of follicular fluid samples aspirated from small (5.0- 6.9 mm), medium (7.0-9.9 mm) and large (≥ 10 mm) follicles of cyclic and acyclic buffaloes (n= 6/group), did not reveal any apparent differences between the groups. DNA fragmentation patterns evaluated by using DNA isolated from the cell pellets obtained after centrifugation of the follicular fluid samples from small-, medium- and largesized follicles of cyclic and acyclic buffaloes (n=5/ group) showed a typical apoptotic oligonucleosome ladder pattern in the acyclic group. In conclusion, these results indicate an increased rate of follicular atresia without any qualitative changes in the follicular fuid proteins during ovarian acyclicity in buffalo.