Aurangābād, India
Aurangābād, India

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Mandage R.H.,Center for Advanced Life science | Jadhavrao P.K.,Abeda Inamdar College | Wadnerkar A.S.,Center for Advanced Life science | Varsale A.R.,Center for Advanced Life science | Kurwade K.K.,Center for Advanced Life science
Journal of Proteomics and Bioinformatics | Year: 2011

The motivation behind the large scale genome analysis of S. mansoni was to explore the possibility of discovering the secretome that is frequently secreted at the interface of parasite and host is supposed to play a crucial role in parasitism by suppressing the immune response, to aid the proliferation of infectivity. Here, we present an efficient pipeline of bioinformatics methodology to identify candidate parasitism proteins within S. mansoni secretome of immune responses in the infected host. The 3,700 proteins deduced from the S. mansoni genome were analysed for the presence or absence of secretory signal peptides. We identified 32 proteins carrying an N-terminal secreted signal peptide but deficient in extra membrane-anchoring moieties. Notably we identified proteins involved in ATP synthesis, redox balance, protein folding, gluconeogenesis, development and signaling, scavenging and nucleotide metabolic pathways, immune response modulation. Most of these proteins define their potential for immunological diagnosis and vaccine design. A systematic attempt has been made here to develop a general method for predicting secretory proteins of a parasite with high efficiency and accuracy. © 2011 Mandage RH, et al.

Dangre D.M.,Maharashtra University of Health Sciences | Deshmukh S.R.,Sant Gadge Baba Amravati University | Rathod D.P.,Sant Gadge Baba Amravati University | Umare V.D.,Center for Advanced Life science | Ullah I.,Gyeongsang National University
Journal of Proteomics and Bioinformatics | Year: 2010

Nanoviridae is a family of single stranded DNA viruses which infect the plants through their phloem tissues. The few members of these viruses are now turning towards animals. It includes two genera, Nanovirus and Babuvirus. Nanovirus includes three species namely Faba beans necrotic yellows virus (FBNYV), Milk vetch dwarf virus (MVDV) and Subterranean clover stunt virus (SCSV) while Babuvirus have two species accounted yet, namely Abaca bunchy top virus (ABTV) and Banana bunchy top virus (BBTV). The viral coat proteins are likely to be responsible for many diseases in plants as well as animals. The intra-genomic changes or post translational modifications with in SCSV have decreased the length of the sequence, but increased the antigenic potential and numbers of antigenic binding regions on the surface of the protein. We have predicted the most probable responsible antigenic determinants and MHC binders within the viruses in Nanoviridae family. MHC molecules play a crucial role in host immune response. The nonamers of antigenic determinants in Nanoviridae family viral coat proteins are highly sensitive to H-2Kd and I-Ag7 molecules. The species within particular genera shows their common epitopes along with diverse colleague epitopes. These common antigenic peptides can be used as their identifiers. These identifiers along with their diverse colleagues could be most informative for antidotes production against themselves. Also, these are important for synthetic peptide vaccine production against the relevant viruses. © 2010 Dangre DM, et al.

Varsale A.R.,Center for Advanced Life science | Wadnerkar A.S.,Center for Advanced Life science | Mandage R.H.,Center for Advanced Life science | Jadhavrao P.K.,Abeda Inamdar College
Journal of Proteomics and Bioinformatics | Year: 2010

Streptococcus mutans is the principal etiological agent of dental caries worldwide and is considered to be the most cariogenic of all of the oral streptococci. We sought to expand our understanding of this organism at the molecular level through identification and function prediction of novel protein domains by two automated approaches using HMM and BLAST to develop a complete set of domains, which were then subsequently manually analysed. A final set of 19 novel domains and families was identified. This enhances our understanding of both S mutans and also general bacterial molecular mechanisms, including protein synthesis to catalytic action. Furthermore we demonstrate that using this type of in silico method it is possible to fairly rapid generation of new biological information from previously uncorrelated data to increase the rate of discoveries in the laboratory. © 2010 Varsale AR, et al.

Joshi A.L.,Center for Advanced Life science | Roham P.H.,Center for Advanced Life science | Mhaske R.,Center for Advanced Life science | Jadhav M.,Center for Advanced Life science | And 3 more authors.
Natural Product Research | Year: 2015

(Graph Presented) Calotropis procera (family: Asclepiadaceae) contains cardiac glycosides which are cytotoxic to cancer cells. The extracts of C. procera have been reported to be cytotoxic to many cancer cell lines and this is the first report against the human skin melanoma cells (SK-MEL-2). The SK-MEL-2 cells treated with C. procera methanolic extract (CPME) were analysed for growth inhibition and apoptosis. The exposure of phosphatidylserine in apoptotic SK-MEL-2 was analysed by using the Annexin-V FITC flow cytometry method. In CPME-treated SK-MEL-2 cells, 19.6% of apoptotic and 58.3% dead cells were observed. The 15.97% and 15.85% of early apoptotic cells were found at 20 μg/mL of the ouabain and paclitaxel, respectively. Active caspases, nuclear degradation confirmed apoptotic SK-MEL-2 cells in time- and dose-dependent manner. The cell cycle analysis shows that CPME treated cells halt at G2/M phase. Significant cytotoxic activity of CPME against SK-MEL-2 may be attributed to its high cardenolide content. © 2015 Taylor & Francis.

Mandage R.H.,Center for advanced Life science | Wadnerkar A.S.,Center for advanced Life science
International Journal of Pharma and Bio Sciences | Year: 2010

Availability of gene and protein sequences of parasite has provided a remarkable amount of data that can be useful in drug target identification and vaccine development. Although extensive researches are on way in order to control the disease caused by eukaryotic parasites and to develop drug(s) against them, till date no effective vaccine or specific drug is available. Subtractive genomics approach is one of the recently adopted methodology in which the subtraction of sequence between the host and parasite proteome provides information for a set of proteins that are likely to be essential to the parasite but absent in the host. We have used the same methodology to analyse the proteome of the human parasite Leishmania donovani.Our analysis showed that out of the 446 protein sequences of the parasite, 29 represent unique to parasite and predicted as putative drug targets. 16 membrane, 5 nuclear, 3 cytoplasmic along with 2 mitochondrial are found to be the potential drug targets by using subtractive genomics approach. The preliminary work presented here identifies a small subset of the L. donovani proteome that might be investigated further for identifying potential drug and vaccine candidate in this parasite.

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