Center Beninois Of La Recherche Scientifique Et Technique Cbrst

Cotonou, Benin

Center Beninois Of La Recherche Scientifique Et Technique Cbrst

Cotonou, Benin
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Kpadonou-Kpoviessi B.G.H.,University Abomey Calavi | Kpadonou-Kpoviessi B.G.H.,Center Beninois Of La Recherche Scientifique Et Technique Cbrst | Kpadonou-Kpoviessi B.G.H.,Laboratoire Detudes Et Of Recherche En Chimie Appliquee Lerca | Kpoviessi D.S.S.,University Abomey Calavi | And 5 more authors.
Journal of Chemical and Pharmaceutical Research | Year: 2013

The antimicrobial and toxicity against Artemia salina Leach of extracts from different parts (stems, leaves and seeds) of Ocimum gratissimum collected at two vegetative stages were analyzed. In addition to essential oils that contain volatile compounds and which antimicrobial properties we studied in a previous work [12], the plant contains less or non-volatile compounds that could explain in part, its properties. The crude ethanol extract of different parts (leaves, stems and seeds in pre- and full flowering) showed yields between 1.97% and 4.81%. Leaves had given more extract than seeds and the lower yield was obtained with the stems. Antimicrobial tests and toxicity against A. salina Leach helped to identify the crude extract of leaves and stems in full flowering as the most active respectively against C. albicans ATCC, and E. coli ATCC and S. aureus ATCC with a good selectivity. Phytochemical screening of different samples revealed the presence of polyphenolic compounds (gallic and catechic tannins, flavonoids, anthocyanes and leucoanthocyanes), quinone derivatives, triterpenoids, steroids, mucilage, coumarins, reducing compounds and essential oils. The liquid-liquid fractionation of the active crude extracts with different solvents of increasing polarity showed ethyl acetate fraction as the most active. This fraction needs to be further investigated by isolation and identification of pure bioactive compounds by bio-guided fractionation. This is the first report of interaction between plant parts, vegetative stages, antimicrobial properties and toxicity of the nonvolatile fractions of O. gratissimum Linn from Benin.


Tokpohozin N.B.,Institute Of Mathematiques Et Of Science Physiques Imsp Uac | Tokpohozin N.B.,Center Beninois Of La Recherche Scientifique Et Technique Cbrst | Kounouhewa B.,Center Beninois Of La Recherche Scientifique Et Technique Cbrst | Kounouhewa B.,Laboratoire Of Physique Du Rayonnement Lpr Fast Uac | And 4 more authors.
Acta Oceanologica Sinica | Year: 2015

Under the action of marine currents, non-cohesive sediments evolve by bed-load, by saltation or suspension depending on their granulometry. Several authors have considered that the movement of sediment is bidimensional and modelized the effects of swell by a constant velocitynear the seabed. Here we have studied the velocity profile of fluctuating currents near the seabed and studied the movement of sediment in 3D. The results show that in the areas of study (surf and swash) the movement of sediment occurs in a volume, and the evolution of sediment varies from an areato another. The obtained theoretical profiles of the position and velocity vectors confirm the observations of several authors. © 2015, The Chinese Society of Oceanography and Springer-Verlag Berlin Heidelberg.


Lagnika L.,University Abomey Calavi | Lagnika L.,Center Beninois Of La Recherche Scientifique Et Technique Cbrst | Anago E.,University Abomey Calavi | Atindehou M.,University Abomey Calavi | And 3 more authors.
African Journal of Biotechnology | Year: 2011

An attempt has been made to carry out a screening on the antibacterial activity of leaves of Crateva religiosa Forst used in Benin traditional veterinary medicine against bacterial infection of Thryonomys swinderianus (class of Mammalia, family of Thryonomyidae) commonly called agouti or kholan. The aim of this study was to select the most active extracts and fractions which may be useful to combat these bacterial infections. Seven extracts from C. religiosa were screened for their antibacterial. The antibacterial activity was evaluated by both microtest method using p-iodonitrotetrazolium and bioautography against five microorganisms obtained from T. swinderianus (Escherichia coli, Shigella sonei, Staphylococcus aureus, Pasteurela pestis and Yersinia enterocolitica). The minimum inhibitory concentration (MIC) and the total activity (TA) were determined. All extracts were effective against tested microorganisms at different levels (0.31 ≤ MIC ≤ 10 mg/ml). The screening experiment revealed that ethyl acetate extract was more potent than other extracts with the MIC values of 0.62 mg/ml against E. coli and 0.31 mg/ml against S. aureus, S. sonei, P. pestis and Y. enterocolitica. The results provide an evidence for the traditional use of C. religiosa for the treatment of infective diseases of T. swinderianus Temminck. © 2011 Academic Journals.


Ahanhanzo C.,University Abomey Calavi | Gandonou C.B.,University Abomey Calavi | Gandonou C.B.,Center Beninois Of La Recherche Scientifique Et Technique Cbrst | Agbidinoukoun A.,University Abomey Calavi | And 2 more authors.
African Journal of Biotechnology | Year: 2010

The effect of two growth regulator combinations was studied on the morphogenesis in vitro of 3 genotypes of yams (Kounondakou, Gnon-boya and RB 89579). Benzyl aminopurin (BAP) and zeatin (ZEAT) were tested, respectively at a concentration of 0.5 mg/l with the galzy glutamine basic medium containing naphtalene acetic acid (NAA) (0.5 mg/l). Stem fragments were used as explants. The number of stripped buds and explants having stem and roots are sampled after 2 weeks in culture. The dry matter content, the number of roots and leaves and the height of each young sprout were determined after 5 weeks in culture. The results obtained indicated that no break in leaf growth was observed on the control medium (without cytokinin) but media with BAP and zeatin presented a good plants aerial part development. A significant interaction (p < 0.05) was observed between the genotypes and the type of cytokinin. However, the highest bud sprouting and shoot development were obtained with BAP. Thus, BAP can be considered as cytokinin having a good morphogenic aptitude when compared to zeatin for yam micropropagation. © 2010 Academic Journals.

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