CENID Microbiologia

Mexico

CENID Microbiologia

Mexico
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Montes-Garcia J.F.,National Autonomous University of Mexico | Vaca S.,National Autonomous University of Mexico | Vazquez-Cruz C.,Institute ciencias | Soriano-Vargas E.,University of Central Mexico | And 3 more authors.
Current Microbiology | Year: 2016

Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149T secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis. © 2015, Springer Science+Business Media New York.


Mastitis is the mammary gland infection, in which more than 130 organisms may be etiologic agents. Subclinical mastitis is the responsible for great economic losses, it present high somatic cell count, decreased milk production without apparent changes. The research was conducted in Marquelia, Guerrero, México. Located at 160 30' North and 980 39' West, with warm humid climate. The Californian Mastitis Test was applied to 181 cows, from 11 production units, they were chosen by simple random sampling, with a confidence level of 95 % and an error margin of 5 %; the sampling frame was the member's list of the Local Breeders' Association of Marquelia. From the positive cows was taken a milk sample, it was inoculated in Blood agar, Mc Conkey agar and Mannitol Salt agar for bacterial isolation. Microbial identification was performed using traditional biochemical tests. The proportion of bacteria found, according to parity, day in milk and milk production was analyzed with the X2 test. The prevalence of subclinical mastitis was 45,9 %. In 60 % of all cases Staphylococcus aureus was isolated; in 33,3 % Staphylococcus coagulase negative, gram negative bacilli (26,7 %) and gram positive bacilli (13,3 %). These proportions were statistically significant (P<0,0001). It was concluded that the prevalence of subclinical mastitis is high, in this region and the main etiologic agent is Staphylococcus aureus.


Fernandez-Rojas M.A.,National Autonomous University of Mexico | Vaca S.,National Autonomous University of Mexico | Reyes-Lopez M.,CINVESTAV | de la Garza M.,CINVESTAV | And 4 more authors.
MicrobiologyOpen | Year: 2014

Pasteurella multocida (Pm) is a gram-negative bacterium able to infect different animal species, including human beings. This bacterium causes economic losses to the livestock industry because of its high morbidity and mortality in animals. In this work, we report the characterization of outer membrane vesicles (OMVs) released into the culture medium by different Pm serogroups. Purified OMVs in the range of 50-300nm were observed by electron microscopy. Serum obtained from chickens infected with Pm recognized several proteins from Pm OMVs. Additionally, rabbit antiserum directed against a secreted protease from Actinobacillus pleuropneumoniae recognized a similar protein in the Pm OVMs, suggesting that OMVs from these bacterial species contain common immunogenic proteins. OmpA, a multifunctional protein, was identified in OMVs from different Pm serogroups, and its concentration was twofold higher in OMVs from Pm serogroups B and D than in OMVs from other serogroups. Three outer membrane proteins were also identified: OmpH, OmpW, and transferrin-binding protein. Three bands of 65, 110, and 250kDa with proteolytic activity were detected in Pm OMVs of serogroups A and E. Additionally, β-lactamase activity was detected only in OMVs from Pm 12945 Ampr (serogroup A). Pm OMVs may be involved in different aspects of disease pathogenesis. © 2014 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.


Suazo F.M.,CENID Fisiologia y Mejoramiento Animal | Harris B.,CENID Fisiologia y Mejoramiento Animal | Diaz C.A.,CENID Microbiologia | Thomsen B.,U.S. Department of Agriculture | And 6 more authors.
Tecnica Pecuaria en Mexico | Year: 2010

Quick diagnosis of tuberculosis in cattle is critical to decide whether or not to quarantine or depopulate a herd. Currently, decisions are based on culture, which takes between four and eight weeks to accomplish. Therefore, the purpose of this study was to evaluate relative sensitivity and specificity of two PCR's (MPB70 and IS6110) and spoligotyping as quick diagnostic tests for cattle tuberculosis in fresh tissue. Fifty tissue samples with TB-suspicious lesions from a herd with 25% prevalence of the disease and fifty tissue samples with no lesions from a TB-free herd were used in the study. Samples were split into two sets and each set was blind analyzed in two different laboratories under the same protocol. The three tests were used to diagnose tuberculosis in all samples using macerated tissue just before culturing. Relative sensitivity and specificity of all tests were estimated using presence/absence of lesion, histopathology and culture results as gold standards. MPB70 nested PCR showed consistently higher sensitivity (85 to 91%) and specificity (77 to 86%) in one of the laboratories with all gold standards; however, inter-laboratory differences occurred, in one of the laboratories sensitivity was from 89 to 91% and specificity from 57 to 63%. IS6110 nested PCR and spoligotyping behaved poorly. Relative sensitivity and specificity for MPB70 nested PCR suggest that this test could be useful as a complementary test for quick diagnosis of bovine TB in fresh tissue.


Suazo F.M.,CENID Fisiologia y Mejoramiento Animal | Harris B.,U.S. Department of Agriculture | Diaz C.A.,CENID Microbiologia | Thomsen B.,U.S. Department of Agriculture | And 6 more authors.
Revista Mexicana De Ciencias Pecuarias | Year: 2010

Quick diagnosis of tuberculosis in cattle is critical to decide whether or not to quarantine or depopulate a herd. Currently, decisions are based on culture, which takes between four and eight weeks to accomplish. Therefore, the purpose of this study was to evaluate relative sensitivity and specificity of two PCR's (MPB70 and IS6110) and spoligotyping as quick diagnostic tests for cattle tuberculosis in fresh tissue. Fifty tissue samples with TB-suspicious lesions from a herd with 25 % prevalence of the disease and fifty tissue samples with no lesions from a TB-free herd were used in the study. Samples were split into two sets and each set was blind analyzed in two different laboratories under the same protocol. The three tests were used to diagnose tuberculosis in all samples using macerated tissue just before culturing. Relative sensitivity and specificity of all tests were estimated using presence/absence of lesion, histopathology and culture results as gold standards. MPB70 nested PCR showed consistently higher sensitivity (85 to 91 %) and specificity (77 to 86 %) in one of the laboratories with all gold standards; however, inter-laboratory differences occurred, in one of the laboratories sensitivity was from 89 to 91 % and specificity from 57 to 63 %. IS6110 nested PCR and spoligotyping behaved poorly. Relative sensitivity and specificity for MPB70 nested PCR suggest that this test could be useful as a complementary test for quick diagnosis of bovine TB in fresh tissue.


PubMed | CENID Microbiologia, National Autonomous University of Mexico, University of Queensland, Institute ciencias and University of Central Mexico
Type: Journal Article | Journal: Current microbiology | Year: 2016

Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149(T) secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.

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