CENID Microbiologia

Mexico

CENID Microbiologia

Mexico

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Suazo F.M.,CENID Fisiologia y Mejoramiento Animal | Harris B.,CENID Fisiologia y Mejoramiento Animal | Diaz C.A.,CENID Microbiologia | Thomsen B.,U.S. Department of Agriculture | And 6 more authors.
Tecnica Pecuaria en Mexico | Year: 2010

Quick diagnosis of tuberculosis in cattle is critical to decide whether or not to quarantine or depopulate a herd. Currently, decisions are based on culture, which takes between four and eight weeks to accomplish. Therefore, the purpose of this study was to evaluate relative sensitivity and specificity of two PCR's (MPB70 and IS6110) and spoligotyping as quick diagnostic tests for cattle tuberculosis in fresh tissue. Fifty tissue samples with TB-suspicious lesions from a herd with 25% prevalence of the disease and fifty tissue samples with no lesions from a TB-free herd were used in the study. Samples were split into two sets and each set was blind analyzed in two different laboratories under the same protocol. The three tests were used to diagnose tuberculosis in all samples using macerated tissue just before culturing. Relative sensitivity and specificity of all tests were estimated using presence/absence of lesion, histopathology and culture results as gold standards. MPB70 nested PCR showed consistently higher sensitivity (85 to 91%) and specificity (77 to 86%) in one of the laboratories with all gold standards; however, inter-laboratory differences occurred, in one of the laboratories sensitivity was from 89 to 91% and specificity from 57 to 63%. IS6110 nested PCR and spoligotyping behaved poorly. Relative sensitivity and specificity for MPB70 nested PCR suggest that this test could be useful as a complementary test for quick diagnosis of bovine TB in fresh tissue.


Suazo F.M.,CENID Fisiologia y Mejoramiento Animal | Harris B.,U.S. Department of Agriculture | Diaz C.A.,CENID Microbiologia | Thomsen B.,U.S. Department of Agriculture | And 6 more authors.
Revista Mexicana De Ciencias Pecuarias | Year: 2010

Quick diagnosis of tuberculosis in cattle is critical to decide whether or not to quarantine or depopulate a herd. Currently, decisions are based on culture, which takes between four and eight weeks to accomplish. Therefore, the purpose of this study was to evaluate relative sensitivity and specificity of two PCR's (MPB70 and IS6110) and spoligotyping as quick diagnostic tests for cattle tuberculosis in fresh tissue. Fifty tissue samples with TB-suspicious lesions from a herd with 25 % prevalence of the disease and fifty tissue samples with no lesions from a TB-free herd were used in the study. Samples were split into two sets and each set was blind analyzed in two different laboratories under the same protocol. The three tests were used to diagnose tuberculosis in all samples using macerated tissue just before culturing. Relative sensitivity and specificity of all tests were estimated using presence/absence of lesion, histopathology and culture results as gold standards. MPB70 nested PCR showed consistently higher sensitivity (85 to 91 %) and specificity (77 to 86 %) in one of the laboratories with all gold standards; however, inter-laboratory differences occurred, in one of the laboratories sensitivity was from 89 to 91 % and specificity from 57 to 63 %. IS6110 nested PCR and spoligotyping behaved poorly. Relative sensitivity and specificity for MPB70 nested PCR suggest that this test could be useful as a complementary test for quick diagnosis of bovine TB in fresh tissue.


PubMed | CENID Microbiologia, National Autonomous University of Mexico, University of Queensland, Institute ciencias and University of Central Mexico
Type: Journal Article | Journal: Current microbiology | Year: 2016

Gallibacterium anatis has the ability to hemagglutinate rabbit erythrocytes; however, no bacterial component has yet been associated with this function. In the present work, a protein of approximately 65 kDa with hemagglutinating activity for glutaraldehyde-fixed chicken erythrocytes was purified by ion interchange chromatography from G. anatis F149(T) secreted proteins. The protein was recognized by a rabbit polyclonal serum against a hemagglutinin from Avibacterium paragallinarum. The 65 kDa purified protein presented identity with a G. anatis filamentous hemagglutinin by mass spectrometric analysis. As well, the bacterial surface of G. anatis was labeled by immune gold assays using a polyclonal serum against the 65-kDa protein. A similar protein was recognized in four other G. anatis strains by immunoblots using the same antiserum. The protein binds sheep or pig biotinylated fibrinogen, suggesting an interaction with basement membrane eukaryotic cells components, and the protein is present in G. anatis biofilms. Overall, the results suggest that the 65 kDa hemagglutinin is a common antigen and a potential virulence factor in G. anatis.

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