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Incheon, South Korea

Patent
Celltrion Inc. | Date: 2010-03-30

The present invention relates to a method of selecting high producer clones by using an expression vector, the expression vector comprising: (i) a gene expression cassette comprising a selectable marker gene to which polyA has been inoperably linked; and (ii) a gene expression cassette which encodes a recombinant protein of interest and to which polyA has been operably linked. According to the invention, high producer clones can be selected from cell populations at least 10 times fewer than in the existing methods of selecting cell lines. Particularly, high producer clones can be selected using a low concentration of MTX compared to a conventional stepwise gene amplification strategy which comprises carrying out multiple amplification steps while increasing the concentration of MTX. Accordingly, the development period of cell lines can be shortened and the labor and cost required for selection of high-productivity cell clones can be reduced, whereby more efficient production of proteins is possible even when general selectable marker genes other than MTX are used.


The present invention relates to a method to improve the sorbent efficiency of protein A chromatography using switching column with continuous feeding. In the chromatography method of the present invention, the increased usage efficiency of the absorbent (resin), the decreased processing time, the decreased operation cycle of column compared to that of single batch-type column, and the reduced amount of used resin are achieved and thus, the target protein can be purified at a high efficiency and a low cost.


Patent
Celltrion Inc. | Date: 2012-01-30

Disclosed is a load limiter mounted within a cylindrical spool on which a webbing is wound to constrain a load applied to the webbing from exceeding a preset load, the load limiter including: a first torsion bar inserted into a hollow portion formed at a center of the spool and one end of which is coupled to one end of the spool; and a second torsion bar one end of which is connected to an opposite end of the first torsion bar to be inserted into the hollow portion of the spool together with the first torsion bar and an opposite end of which is coupled to a locker installed at an opposite end of the spool, wherein the torsion bar is configured such that a torsion load limit of the first torsion bar is smaller than a torsion load limit of the second torsion bar.


The present invention relates to an antibody-linker-drug conjugate in which an antibody and a cytotoxic drug are conjugated through an enzyme cleavable peptide linker capable of directly binding to a lysine residue of an antibody, a preparation method therefor, and an anticancer drug composition containing the same as an active ingredient.


The present invention provides a dolastatin-10 derivative having excellent anticancer activity, a method of producing the same and anticancer drug composition containing the same as an active ingredient.

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