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Bernried, Germany

Neugebauer U.,University Hospital Jena | Neugebauer U.,Institute of Photonic Technology | Grosse C.,University Hospital Jena | Grosse C.,Institute of Photonic Technology | And 17 more authors.
Biomedizinische Technik | Year: 2012

Infections, particularly those due to drug-resistant pathogens, significantly increase morbidity and mortality as well as cost of treatment and length of hospital stays. Staphylococcus aureus, a highly human-adapted organism, is the most common pathogen causing nosocomial infections. Among S. aureus, especially methicillin-resistant S. aureus (MRSA) causes problems in therapy and infection control. Understanding the mechanisms of infections is as important as the development of rapid tools for diagnosis. Within the Photonics4Life project "From Infection to Detection" these two goals are addressed. Modern optical technologies, such as multi-focus quantitative digital holographic microscopy (DHM) phase contrast, holographic optical tweezers (HOT) and Raman spectroscopy have been employed to analyse the cell morphology, cell dynamics and cellular refractive index of endothelial cells before and after incubation with S. aureus (or with model bacteria for HOT and DHM analysis). Individual bacteria inside the cells have been visualized and defined infection scenarios at the single cell level could be created. Finally, optical techniques were evaluated for further sub-typing of S. aureus strains and compared to the established spa typing method. © 2012 by Walter de Gruyter Berlin Boston. Source


Kemper B.,University of Munster | Langehanenberg P.,University of Munster | Hoink A.,University of Munster | von Bally G.,University of Munster | And 7 more authors.
Journal of Biophotonics | Year: 2010

For a precise manipulation of particles and cells with laser light as well as for the understanding and the control of the underlying processes it is important to visualize and quantify the response of the specimens. Thus, we investigated if digital holographic microscopy (DHM) can be used in combination with microfluidics to observe optically trapped living cells in a minimally invasive fashion during laser micromanipulation. The obtained results demonstrate that DHM multi-focus phase contrast provides label-free quantitative monitoring of optical manipulation with a temporal resolution of a few milliseconds. Laser micromanipulation monitoring of optically trapped pancreas tumor cells by quantitative digital holographic phase contrast. The arrows in the false colour coded quantitative phase contrast images indicate the impact of the treatment with focussed laser light. © 2010 by WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Bendrat K.,Pathologie Hamburg West | Bendrat K.,Universitatsklinikum Hamburg Eppendorf | Stang A.,Asklepios Klinik Barmbek | Georgiev G.,Metalife AG | And 7 more authors.
Journal of Biophotonics | Year: 2012

Although it is increasingly recognized that the tumor biology is influenced by the tumor stroma, prognostic gene signatures are usually derived from tissue consisting of tumor cells and surrounding stroma. This study presents a compartment-specific transcriptome analysis of lung squamous cell carcinoma (SCC) samples microdissected into tumor parenchyma and stroma fractions. Typical tumor and stroma genes were identified based on the expression ratios between the two compartments. Our results indicate that in SCC many markers related to longer survival are predominantly expressed in the stroma, particularly genes of the MHC-II complex. Stromal upregulation of MHC-II genes seems crucial for a clinically relevant antitumor immune response in SCC. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Source


Grant
Agency: Cordis | Branch: H2020 | Program: SME-1 | Phase: PHC-12-2014-1 | Award Amount: 71.43K | Year: 2015

Biomarkers in discovery are valuable tools to understand the pathobiology of a disease and the pharmacology of a target under investigation. In-vitro screen would be invaluable in identifying working hypothesis early in drug development process. Information provided by properly selected biomarkers can greatly influence a go/no go decision. The challenge is to identify relevant biomarkers early enough to implement them for such critical decisions. In developing in-vitro assays to identify biomarkers with potential clinical application and utility, a clear understanding and determination of what that biomarker will assess must be defined. More reliable biomarker screening protocols based on allometric scaling laws are needed to be implemented in order to gain results more respondent to human physiology. The predictive power of High Content Screening techniques can be enhanced by working with primary cells or differentiated stem cells and in 3-D culture. Nevertheless, in-vitro cell cultures are still far away from the complexity of human tissues or organs. IVTech intends to industrialize and test Multidyn, a patented advanced low-cost cell culture system made up by a 24-chambers plate complying with the multiwell standard, capable of any internal connection and flow within the cells, able to realize cheaper faster in-vitro physiologically relevant cellular systems. Multidyn will be tested for early diabetes biomarker discovery by using a developed model of liver, vascular endothelium and adipose tissues, and when equipped with the highly performing 3D scaffolds provided by the partner Scaffdex and with suitable High Content Imaging systems provided by the partner Celltool and selected scientific laboratories. IVTech Multidyn is at TRL 6. Phase 1 project will assess the activities needed to demonstrate the product in operational environment with potential end-users, establish a large scale production and test the market.


Koch S.,CellTool GmbH | Walles H.,University of Wurzburg | Krause K.H.,University of Geneva | Baquie M.,NEURIX SA | And 2 more authors.
Progress in Biomedical Optics and Imaging - Proceedings of SPIE | Year: 2013

Raman spectroscopy increasingly becomes a valuable analytical tool in biomedicine. A novel Raman microscope designed for biomedical applications was used to discriminate viability states and cell types of Hodgkin's disease as well as different neural and invading glioblastoma cells within a human engineered neural tissue (ENT).© 2013 OSA-SPIE. Source

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