Frezzetti D.,Cell Biology And Biotherapy Unitistituto Nazionale Tumori Fondazione scale Irccsnaplesitaly |
Gallo M.,Cell Biology And Biotherapy Unitistituto Nazionale Tumori Fondazione scale Irccsnaplesitaly |
Roma C.,Laboratory Of Pharmacogenomicscentro Of Ricerche Oncologiche Of Mercogliano Crom Instituto Nazionale Tumori Fondazione G Pascale Irccsnaplesitaly |
D'Alessio A.,Cell Biology And Biotherapy Unitistituto Nazionale Tumori Fondazione scale Irccsnaplesitaly |
And 4 more authors.
Journal of Cellular Physiology | Year: 2015
Vascular endothelial growth factor A (VEGFA) is one of the main mediators of angiogenesis in non-small cell lung cancer (NSCLC). Recently, it has been described an autocrine feed-forward loop in NSCLC cells in which tumor-derived VEGFA promoted the secretion of VEGFA itself, amplifying the proangiogenic signal. In order to investigate the role of VEGFA in lung cancer progression, we assessed the effects of recombinant VEGFA on proliferation, migration, and secretion of other angiogenic factors in A549, H1975, and HCC827 NSCLC cell lines. We found that VEGFA did not affect NSCLC cell proliferation and migration. On the other hand, we demonstrated that VEGFA not only produced a strong and persistent increase of VEGFA itself but also significantly induced the secretion of a variety of angiogenic factors, including follistatin (FST), hepatocyte growth factor (HGF), angiopoietin-2 (ANGPT2), granulocyte-colony stimulating factor (G-CSF), interleukin (IL)-8, leptin (LEP), platelet/endothelial cell adhesion molecule 1 (PECAM-1), and platelet-derived growth factor bb (PDGF-BB). PI3K/AKT, RAS/ERK, and STAT3 signalling pathways were found to mediate the effects of VEGFA in NSCLC cell lines. We also observed that VEGFA regulation mainly occurred at post-transcriptional level and that NSCLC cells expressed different isoforms of VEGFA. Collectively, our data suggested that VEGFA contributes to lung cancer progression by inducing a network of angiogenic factors, which might offer potential for therapeutic intervention. © 2015 Wiley Periodicals, Inc.