Jin H.,Cell Biology and Biochemistry Group |
Yu Y.,Cell Biology and Biochemistry Group |
Chrisler W.B.,Cell Biology and Biochemistry Group |
Xiong Y.,Cell Biology and Biochemistry Group |
And 2 more authors.
Breast Cancer: Basic and Clinical Research | Year: 2011
Recent studies revealed that micro RNA-10b (mir-10b) is highly expressed in metastatic breast cancer cells and positively regulates breast cancer cell migration and invasion through inhibition of HOXD10 target synthesis. In this study we designed anti-mir-10b molecules and combined them with poly L-lysine (PLL) to test the delivery effectiveness. An RNA molecule sequence exactly matching the mature mir-10b minor antisense showed strong inhibition when mixed with PLL in a wound-healing assay with human breast cell line MDA-MB-231. The resulting PLL-RNA nanoparticles delivered the anti-microRNA molecules into cytoplasm of breast cancer cells in a concentration-dependent manner that displayed sustainable effectiveness. © the author(s), publisher and licensee Libertas Academica Ltd. Source