Lee E.,Kyung Hee University |
Kim D.Y.,Kyung Hee University |
Kim D.Y.,Cell and Bio Inc |
Chung E.,Kyung Hee University |
And 4 more authors.
Cell Transplantation | Year: 2014
Mechanical stimulation is a known modulator of survival and proliferation for many cells, including endothelial cells, smooth muscle cells, and bone marrow-derived mesenchymal stem cells. In this study, we found that mechanical strain prevents apoptosis and increases the adhesive ability of dermal fibroblasts in vitro and thus confers the survival advantage in vivo after transplantation of fibroblasts into the full-thickness wound of diabetic mice. Cyclic stretch at a frequency of 0.5 Hz and maximum elongation of 20% stimulates cellular survival mediated by the activation of extracellular signal-regulated kinases (ERKs), c-Jun N-terminal kinases (JNKs), and the serine/threonine kinase Akt (AKT). Stretching of the fibroblasts increases the synthesis of extracellular matrix proteins and the formation of denser focal adhesion structures, both of which are required for fibroblast adhesion. The stretched fibroblasts also upregulate the expression of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α), which enhanced wound healing in vivo. Indeed, preconditioning with mechanical stretch allows better survival of the transplanted fibroblasts, when compared to unstretched control cells, in the wound environment of mice with streptozotocin-induced diabetes and thus accelerates the wound-healing process in these mice. © 2014 Cognizant Comm. Corp.
Chi G.F.,Kyung Hee University |
Choi H.,Kyung Hee University |
Jiang M.H.,Kyung Hee University |
Jiang M.H.,Cell and Bio Inc |
And 3 more authors.
Tissue Engineering and Regenerative Medicine | Year: 2011
In this study, we tested whether the subcutaneous tissue and/or dermis retain inherently the spheroid forming cells or those spheroids are induced by the special induction condition and furthermore explored how much distinct or similar to neurospheroid derived from neural stem cells in the hippocampus. Based on immunofluorescence staining, nestin and p75 positive cells were abundant in both skin appendages of subcutaneous tissue and dermal compartment of neonatal rat skin and could generate numerous spheroids upon the incubation with bFGF and EGF containing spheroid inducing medium. Those spheroids were all identical in gene expressions of Twist, Slug, Sox9, Sox2, nestin, p75, and ABCG2. However, spheroids derived from the hippocampus, which were featured by the lack of p75 and fibronectin expression, were quite distinct from those from subcutaneous and dermis tissue. In the spheroid, nestin and p75 were initially expressed only in the central part of the spheroids but later became positive in all cells of the spheroids after two week culture and the cell proliferation was restricted within the spheroid structure. By neural differentiation and Schwann cell differentiation, the spheroid forming cells could be differentiated to β tubulin- III positive neural lineage cell and Sox10, GFAP positive Schwann cells, respectively. Therefore, our findings indicate that the subcutaneous tissue of neonatal rat skin comprised of numerous spheroid forming cells, possibly similar to neural crest derived stem cells and may be an alternative new source of neural and glial cell lineages for peripheral and central nervous system disease or disorders.
Hong H.-S.,Kyung Hee University |
Hong H.-S.,Cell and Bio Inc. |
Kim D.Y.,Kyung Hee University |
Kim D.Y.,Cell and Bio Inc. |
And 2 more authors.
Archives of Pharmacal Research | Year: 2011
Bone marrow is a reservoir for hematopoietic stem cells, endothelial precursor cells, and bone marrow stromal cells (also generally called mesenchymal stem cells), whose positive role in tissue repair is highly anticipated. In this report, we introduce a novel function of substance-P (SP), an 11-amino-acid peptide, as an injury-inducible messenger to mobilize bone marrow stem cells to the blood and finally to engage in tissue repair. This new drug may substitute for ex vivo cell culture of therapeutic cells by stimulating cell proliferation in the bone marrow in vivo and mobilizing those therapeutic cells to the patient's own blood stream. Again, the additional role of SP in mitigating inflammation-mediated tissue damage can further rationalize the clinical development of SPpeptide as a stem cell stimulant.