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Boly R.,Institute for Research in Health science IRSS CNRST | Franck T.,University of Liege | Kohnen S.,Celabor Scrl | Lompo M.,Institute for Research in Health science IRSS CNRST | And 5 more authors.
Evidence-based Complementary and Alternative Medicine | Year: 2015

The ethyl acetate and n-butanolic subfractions of Agelanthus dodoneifolius were investigated for their antioxidant and antimyeloperoxidase (MPO) activities. The reactive oxygen species (ROS) generation was assessed by lucigenin-enhanced chemiluminescence (CL) and dichlorofluorescein- (DCF-) induced fluorescence techniques from phorbol myristate acetate- (PMA-) stimulated equine neutrophils and human myeloid cell line HL-60, respectively. In parallel, the effects of the tested subfractions were evaluated on the total MPO release by stimulated neutrophils and on the specific MPO activity by means of immunological assays. The results showed the potent activity of the butanolic subfraction, at least in respect of the chemiluminescence test (IC50 = 0.3 ± 0.1 μg/mL) and the ELISA and SIEFED assays (IC50 = 2.8 ± 1.2 μg/mL and 1.3 ± 1.0 μg/mL), respectively. However, the ethyl acetate subfraction was found to be the most potent in the DCF assay as at the highest concentration, DCF fluorescence intensity decreases of about 50%. Moreover, we demonstrated that the ethyl acetate subfraction was rich in catechin (16.51%) while it was not easy to identify the main compounds in the butanolic subfraction using the UPLC-MS/MS technique. Nevertheless, taken together, our results provide evidence that Agelanthus dodoneifolius subfractions may represent potential sources of natural antioxidants and of antimyeloperoxidase compounds. Copyright © 2015 Rainatou Boly et al.

Melillo De Magalhaes P.,Institute Des Science Of La Vie | Melillo De Magalhaes P.,University of Campinas | Dupont I.,Institute Des Science Of La Vie | Hendrickx A.,Institute Des Science Of La Vie | And 5 more authors.
Food Chemistry | Year: 2012

In an attempt to understand the beneficial health effects of Artemisia annua other than its anti-malaria properties, extracts from different cultivars prepared as tea infusions were investigated using Caco-2 cells on the intestinal inflammation and cytochrome P450 (CYP) activities. The characterisation of their phenolic compound (PC) profile revealed rosmarinic and chlorogenic acids as the main PCs. The extracts, assayed on Caco-2 cells at a plausible intestinal concentration, significantly decreased the secretion of pro-inflammatory cytokines, IL-8 and IL-6. This effect could be attributable at least to their content in rosmarinic acid, detected as a potent anti-inflammatory compound. The extracts also inhibited the activity of CYP3A4, whose expression was induced by 1,25-dihydroxyvitamin D3, and of CYP1A1, induced by benzo(a)pyrene. Our results highlight the advantage of drinking A. annua infusions for their potent anti-inflammatory effect, linked to PC content, which could synergise their antimalarial activity. © 2012 Elsevier Ltd. All rights reserved.

Boly R.,Free University of Brussels | Boly R.,Institute for Research in Health science IRSS CNRST | Dessy S.,Celabor Scrl | Kohnen S.,Celabor Scrl | And 8 more authors.
International Journal of Molecular Medicine | Year: 2011

Agelanthus dodoneifolius DC Danser (Loranthaceae) is used for the treatment of various diseases including asthma. The aqueous and hydroalcoholic extracts have been reported to have anti-inflammatory, spasmolytic and bronchorelaxant activities. The present study investigates the effects of the aqueous decoction and the diethyl ether, ethyl acetate and butanolic fractions of Agelanthus dodoneifolius DC Danser (Loranthaceae) on reactive oxygen species (ROS) production and myeloperoxidase (MPO) release by phorbol 12-myristate 13-acetate (PMA)-stimulated equine neutrophils and on purified equine MPO activity. ROS production and MPO release by the PMA-stimulated neutrophils were measured by the lucigenin-enhanced chemiluminescence and ELISA assays, respectively. Specific immunological extraction followed by enzymatic detection (SIEFED) was used to specifically measure the equine MPO activity. Identification and quantification of the individual and total phenolic and flavonoid compounds were performed using UPLG-MS/MS equipment and colorimetric methods involving Folin-Giocalteu and AlCl3, respectively. All the tested extracts displayed dose-dependent inhibitory effects on the oxidant activities of neutrophils; a stronger effect was observed with the organic fractions than the aqueous decoction. These findings could be correlated with a high content of phenolic and flavonoid compounds. The results confirm the previously shown anti-inflammatory effect of Agelanthus dodoneifolius and its potential use for the treatment of neutrophil-dependent inflammatory diseases.

Sergent T.,Institute Des Science Of La Vie | Kohnen S.,Celabor Scrl | Jourez B.,Laboratory of Wood Technology | Beauve C.,Celabor Scrl | And 2 more authors.
Wood Science and Technology | Year: 2014

Robinia pseudoacacia L. heartwood is characterized by a very high natural durability. However, a significant difference was observed between the mature and juvenile heartwood, the latter presenting less durability against fungi decay, which could be attributed to lower extractive content. In order to elucidate this idea, extractives from mature and juvenile heartwoods of black locust trees were investigated. Results showed that extractive and phenolic contents were higher in mature than in juvenile heartwoods. The identification of phenolic compounds by UPLC-DAD-MS/MS revealed, for the first time, the presence of resveratrol and piceatannol. These two stilbenes as well as the flavonoid dihydrorobinetin were present at the highest level in mature heartwood, and as they are known antifungals, they could account for the great durability of mature heartwood. The stilbenes were detected in significant amounts particularly in mature heartwood where piceatannol reached a level tenfold higher than that reported for Japanese knotweed roots, the primary natural source of these stilbenes, whereas resveratrol level was comparable with reported values. As resveratrol and piceatannol receive increasing demand for nutraceutical, cosmetic and, possibly, pharmaceutical purposes, due to their beneficial health effects, this study underlines the use of R. pseudoacacia as a promising sustainable and economical source of resveratrol and piceatannol. © 2014 Springer-Verlag Berlin Heidelberg.

Michiels J.-F.,Catholic University of Louvain | Barbau J.,Catholic University of Louvain | De Boel S.,Catholic University of Louvain | Dessy S.,Celabor Scrl | And 2 more authors.
Process Biochemistry | Year: 2011

While bacteria and yeast are used for the production of rather simple proteins, mammalian cells are generally required for the production of more complex proteins (i.e. post-translational modifications and, in particular, complex glycosylations). In order to boost cell growth and protein production, cultivation media are commonly supplemented with plant peptones, which are hydrolysed plant proteins. Therefore, plant peptones consist mainly of an undefined mixture of peptides, but also contain carbohydrates, phenolic compounds, salts, etc. To increase biosafety, the current trend is the use of chemically defined media. The straightforward idea that only a small number of compounds from peptones are biologically active is attractive, but their identification remains challenging. We first characterised global chemical families of compounds present in a soy peptone. The main constituents of this batch were peptides (60%) and carbohydrates (20%). The addition of this peptone or derived fractions to the culture medium was then tested on Chinese hamster ovary cells, CHO-320 line, expressing γ-interferon (γ-IFN), cultivated in BDM. Its presence did not increase the time-integral of the viable cell density, it increased slightly that of the γ-IFN concentration, but significantly the product/cell yield. Upon fractionation of this peptone by anion exchange chromatography and gel filtration, beneficial and detrimental fractions were obtained. The antioxidant activity of detrimental fractions was higher than that of beneficial fractions. Whereas the chemical nature of antioxidants contained in the detrimental fractions remains unknown, it may be assumed that they could inhibit the proliferation of CHO cells. Although the beneficial compounds were not identified, a fraction exhibiting the same effect as the peptone, but less complex, was obtained. © 2010 Elsevier Ltd.

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