Shivnaz,CMJ University |
Neeraj T.,Ccsuniversity |
Anand,Singhania University |
International Journal of Drug Delivery | Year: 2011
candida albicans is an Opportunistic human pathogen causes both superficial and life threatening mycoses fatal for immuno comprised patients. Many essential oils of spices possess antimycotic properties. The electrochemical gradient generated by PM-H+ATPase of C. albicans drives nutrient transport. H+ ATPase is associated with yeast to hyphal transition, later form being more pathogenic. It is thus important to identify spice extracts which apart from having low MIC also have a profound effect on H+ ATPase. With this in view we have investigated Eugenol alone and along with nutrients (xylose and glucose) on H+ ATPase functioning through H+ extrusion measurement. The antifungal activity of Eugenol alone and along with nutrients (xylose and glucose)was investigated by studying their effect on PM-ATPase mediated H+-Extrusion activity (H+ efflux) and growth of C. albicans (Disc diffusion and MIC).Eugenol inhibited the H+ ATPase as observed by H+ efflux monitored for 30 min. The oil has clear inhibitory effect on H+ efflux in the concentration range of 500μg/ml to 2000μg/ml. Eugenol has an inhibitory effect on growth and H+-Extrusion of H+ ATPase of C. albicans. Both glucose and xylose along with Eugenol have inhibitory effect on Candida growth but no such effect has been observed on H+-ATPase pump of the fungus. Eugenol has a potential to be exploited as future antifungal drug to target plasma membrane H+ ATPase of C. albicans and other pathogenic microbes.
Bhardwaj J.,Sardar Vallabhbhai Patel University of Agriculture and Technology |
Yadav R.,Sardar Vallabhbhai Patel University of Agriculture and Technology |
Ford R.,University of Melbourne |
Yadav R.,Ccsuniversity |
And 3 more authors.
Indian Journal of Genetics and Plant Breeding | Year: 2014
Microsatellites have become a marker of choice in plant genetics and breeding research due to their unilocus, multiallelic and highly reproducible nature. In present study EST sequences isolated from chickpea root under water stress were assessed for the frequency, type and distribution of SSR motifs. Out of the 6400 ESTs (accession no. CDO 38847 to GR 394575), SSR sequences were identified in 348 singleton contigs and from these, 50 flanking primer pairs were designed. Out of these, 41 amplified a marker of the expected size range across nineteen diverse chickpea accessions. 11 of the primer pairs were size polymorphic with an average PIC value of 0.59 across the nineteen genotypes assessed. © 2014, Indian Society of Genetics and Plant Breeding. All rights reserved.
Kumar S.,Ccsuniversity |
Advances in Intelligent and Soft Computing | Year: 2012
Present paper deals with a multi-objective transportation problem. This problem has equality type constraints and some non-commensurable and conflicting objectives. The above objectives are fuzzy in nature. The problem has been solved by three methods using fuzzy programming approach. A numerical example is also given to compare the results obtained by different methods. © 2012 Springer India Pvt. Ltd.
Verma Y.,Ccsuniversity |
Toxicological and Environmental Chemistry | Year: 2014
The levels of cadmium (Cd), chromium (Cr), and copper (Cu) were assessed in 24 fruit species in Meerut, North India using atomic absorption spectrometry. Data showed that Cd concentrations in fruits except banana, pomegranate, papaya, orange, and cherry were above the recommended maximum allowable limit of Food and Agriculture Organization (FAO)/World Health Organization (WHO) (0.2 µg/g). Average Cr concentrations in all analyzed fruit samples were also found higher than the safe limit of FAO/WHO (2.3 µg/g). In contrast, fruits viz. banana, lychees, papaya, Indian apple, Asian apple, and tiger nut showed concentration of Cu below the recommended safe limit (40 µg/g). Our study demonstrated that concentration of studied heavy metals in all tested samples varied according to fruit species and respective contaminants. Data suggest that more strict rules/standards need to be applied by National/International regulatory agencies in order to make these important fruit items free from heavy metals contamination and protect the consumer. © 2015 Taylor & Francis.