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Uppal Kalan, India

Singh S.K.,W309 | Sundaram C.S.,CCMB | Shanbhag S.,W309 | Idris M.M.,W309
Zebrafish | Year: 2010

Zebrafish is one of the most widely studied model organisms for understanding the neurodevelopment and neurological disorders of humans because of its similar brain structure, genome, and proteome. Understanding the zebrafish brain proteome is particularly useful as no data of zebrafish brain proteome are available despite its wide use as an alternative neurological animal model. We have determined the proteome profile of the zebrafish brain based on two-dimensional gel electrophoresis covering 161 proteins including 96 protein identities reported to the Swiss-Prot database. The proteins identified in this study were found to be associated with various pathways such as cell death, free radical scavenging, cell signaling, nervous system development, and cell cycle. The identified proteins were also observed to play important roles in various brain-related diseases and genetic disorders, such as Huntington's disease, Parkinson's disease, and schizophrenia. This article provides the zebrafish brain two-dimensional gel electrophoresis proteome map and the details of the 161 brain tissue-specific proteins. Also we have established the roles of the identified proteins in various neurological functions and diseases based on pathway analysis. © Copyright 2010, Mary Ann Liebert, Inc.

Singh S.K.,CCMB | Rakesh K.S.,CCMB | Ramamoorthy K.,CCMB | Saradhi A.V.P.,CCMB | Idris M.M.,CCMB
Journal of Proteomics and Bioinformatics | Year: 2010

The zebrafish (Danio rerio) is the extensively used alternate vertebrate model animal for understanding the brain function, development and evolution. Detailed brain proteome map of zebrafish is still not known inspite of its broad usage in developmental and neurological studies. We present here the large scale proteome profile of the zebrafish brain at the normal condition based on gel LC ESI MS/MS analysis. A total of 8475 different proteins details were identified based on this study with less than 1% false positive rate. All the proteins details obtained from this study were duly submitted to the database for validation and obtained accession numbers. The various proteins identified in this study were found to be involved in different biological activities, neurological functions and network pathways. With the availability of genomics information, this extensive study of proteomic profile of zebrafish brain tissue provided a complete view and details about the various proteins expressed in brain at the basal state. This study can lead to understand various new biomarkers underlying for various biological characteristics like development and neurological disease. © 2010 Singh SK, et al.

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