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Nova Londrina, Brazil

Vettorato E.D.,Medico Veterinario Autonomo | Da Costa M.C.,University of Guelph | Da Costa Flaiban K.K.M.,State University Londrina | Vidotto O.,CCA UEL | And 2 more authors.
Semina:Ciencias Agrarias | Year: 2012

Previous studies showed that Nelore calves' active production of immunoglobulins is delayed compared to Canchim and Limousin calves, characterizing a possible physiologic difference between taurine and Zebu calves. In this study we compared serum proteins of Nelore and Holstein calves from birth to six months old. Blood samples were taken from 50 healthy calves (30 Nelore and 20 Holstein) in the following ages: 24 to 36 hours of life, and 15, 30, 60, 90, 120, 150 and 180 days. Serum total protein concentrations were measured by colorimetry; albumin, alfa, beta and gammaglobulin concentrations were measured by agarose gel electrophoresis; and IgG concentrations were estimated by the zinc sulphate turbidity test. A repeated measure ANOVA was used to test age effect. The passive transfer of immunity was successful in both breeds and the variation of serum proteins followed the normal pattern throughout the first six months of life. There were breed differences in serum gammaglobulin concentrations. The levels of gammaglobulin were higher at the end of the first day of life, and decreased to the lowest levels at 60 days in Nelore calves and 30 days in Holstein calves. The increase due to the active production of antibodies was precocious in Holstein calves (beginning at 60 days) and slower in Nelore calves (beginning at 90 days), demonstrating that Zebu calves can, in fact, develop active immunity capability later than taurine calves. Source


Caviglione J.H.,Agricultural Institute of Parana | Fonseca I.C.B.,CCA UEL | Filho J.T.,CCA UEL
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2013

Studies on hydrology, agro-meteorology, soil loss and climate change scenarios depend on weather information, which may not be available. Weather generators, such as the CLIGEN, can synthesize daily climate series statistically similar to the observed data. The objective of this study was to evaluate the CLIGEN in generating series in the climatic conditions of Paraná, Brazil, which show transition between Cfa and Cfb climates. Observed data from 20 weather stations from 1975 to 2009 were compared with synthetic series generated with the same number of years. Mean and standard deviation of the number of wet days, daily precipitation, normalized storm peak intensity, solar radiation, maximum and minimum temperatures and dew point were analysed. The coefficient of determination was less than 0.91 in two stations.Under the evaluated conditions, the CLIGEN showed restrictions to simulate the normalized storm peak intensity and, for the remaining variables, it was shown to be viable to synthesize daily climate series statistically similar to those in the observed data. Source


Bernd L.P.,IFRS | Souza T.M.,FAPC OSU | de Oliveira M.A.,CCA UEL | Elisabete Y.S.,DBBTEC CCE UEL | And 2 more authors.
Revista Brasileira de Engenharia Agricola e Ambiental | Year: 2014

The study aimed to evaluate the effect of inoculation of seeds with Pseudomonas fluorescens and NPK fertilization (nitrogen, phosphorus and potassium) on chemical composition and contamination by fungal-fumonisin of maize grains. The experiment was conducted in randomized block design in factorial 2 × 3, corresponding to inoculation with P. fluorescens (non-inoculated or inoculated) and levels of NPK fertilization (0, 125 and 250 kg ha-1, formulation 8-28-16), with 4 replications. The parameters evaluated were chemical composition (protein and ash), contamination by fungal (mold and yeast) and fumonisins B1 (FB1) and B2 (FB2). The data were subjected to analysis of variance, comparisons of means by Tukey test and Pearson correlation by test-t (p < 0.05). Inoculation with P. fluorescens and mineral fertilizer as an isolated factor did not affect the parameters evaluated (p > 0.05). The interaction between factors, increase in NPK level and use of P. fluorescens, resulted in increase in protein content and ash (p < 0.05), and reduction in contamination by yeast of the grain, favoring the growth of Fusarium sp. (r =-0.40, p < 0.05). The levels of total fumonisins (FB1+FB2) observed are above the maximum tolerated limit established by health surveillance agencies. © 2014, Departamento de Engenharia Agricola - UFCG/Cnpq. All rights reserved. Source


Da Costa L.,State University Londrina | Caldart E.T.,State University Londrina | Ruffolo B.B.,State University Londrina | Dos Santos Toledo R.,State University Londrina | And 5 more authors.
Semina:Ciencias Agrarias | Year: 2016

The leishmaniasis are a group of globally widespread parasitic diseases caused by a protozoa of the genus Leishmania, the state of Paraná accounts for 98% of leishmaniasis human cases in southern Brazil and the dogs might serve as a sentinel of the risk of infection to humans in a given area. The aim of this work was to study the epidemiology of leishmaniasis in dogs residents in two different environments, they are: recycling of solid waste centers (Group 1) and a neighborhood adjacent to a forest (Group 2), both in Londrina, Paraná State, Brazil and to determine epidemiological variables associated to infection in these different realities. Serum samples, randomly collected, from 61 dogs from Group 1 and from 74 dogs from Group 2 were submitted to Indirect Immunofluorescence Antibody Test (IFAT) to research for anti-Leishmania IgG antibodies. Of the 135 dogs analyzed, 13 animals (9.6%) were positive; nine (14.7%) in Group 1 and four (5.4%) in Group 2, the difference between the groups is not statistically significant. These results suggest the presence of the protozoan Leishmania spp. in both environments studied which indicate potential for infection in the human population. Source

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