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Han X.,Peking Union Medical College | Ruan J.,Peking Union Medical College | Zhang W.,Peking Union Medical College | Zhou D.,Peking Union Medical College | And 4 more authors.
Oncotarget | Year: 2017

Background: Recent studies have suggested that variables related to host adaptive immunity and the tumor microenvironment may predict the outcome in patients with non-Hodgkin's lymphoma. This study was undertaken to determine the prognostic value of peripheral blood leucocyte subpopulations in diffuse large-B-cell lymphoma patients. Methods: We prospectively analyzed the 16 leukocyte subpopulations using Cytodiff flow cytometric technique in a cohort of 45 diffuse large-B-cell lymphoma patients at a single institution between February and December 2014. The Cox proportional hazards model was used to evaluate prognostic factors for overall survival and progression free survival. Results: Diffuse large-B-cell lymphoma patients had decreased cytotoxic and non-cytotoxic NK&T cells as well as increased CD16+ monocytes, CD16- monocytes and mature neutrophils. The decreased CD16- monocyte/CD16+ monocyte ratio and increased mature neutrophil/cytotoxic NK&T cell ratio were related to poor progression-free and overall survival outcome in single and multivariate analysis. The co-constructed model using International Prognostic Index and mature neutrophil/ cytotoxic NK&T cell ratio can also help discriminate the clinical outcome. Conclusions: The decreased CD16-monocyte/CD16+ monocyte ratio and increased mature neutrophil/cytotoxic NK&T cell ratio predict poor prognosis in diffuse large- B-cell lymphoma patients. This finding provides a strong rationale for the study of cellular immunotherapy in B-cell lymphoma. © Han et al.


PubMed | University of Arkansas for Medical Sciences, University of Liverpool, University of Houston, Woodland Hills Medical Center and 8 more.
Type: Journal Article | Journal: European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery | Year: 2016

Paraneoplastic syndromes are associated with a variety of malignant neoplasms and are systemic and non-metastatic manifestations that develop in a minority of cancer patients. This review examines all published cases of paraneoplastic syndromes associated with neuroendocrine carcinomas of the larynx. There are a total of ten patients reported with paraneoplastic syndromes associated with laryngeal neuroendocrine carcinomas in the literature. Of these, nine died and the tenth is alive with liver metastases. There were five cases of small-cell neuroendocrine carcinoma, four cases of moderately differentiated neuroendocrine carcinoma, and one case of well-differentiated neuroendocrine carcinoma associated with paraneoplastic syndromes. As these syndromes have significant clinical relevance, physicians should be aware of the possible presence of paraneoplastic syndromes in the diagnostic process of patients with neuroendocrine carcinoma of the larynx.


Wang F.,Peking Union Medical College | Xu D.,Cblpath Inc. | Cui W.,Peking Union Medical College
Clinica Chimica Acta | Year: 2015

Background: CD45 is universally expressed on nucleated hematopoietic cells in healthy individuals with variable intensities. Although down-regulation of CD45 expression is commonly seen in immature B-cell neoplasm, loss of CD45 expression is rarely observed in mature B-cell lymphoproliferative disorders. Case report: A 64-y old man was presented with fatigue and night sweat in July 2013. Physical examination revealed right axillary lymphadenopathy without splenohepatomegaly. The lymph node was sent for flow cytometry and histomorphologic evaluation. Flow cytometric analysis revealed the presence of CD45. + and CD45. - cell populations. The CD45. + population (42%) comprised mainly unremarkable CD3. + T cells. However, the CD45. - population (35%) showed CD19. +, CD20. +, and CD10. + immunophenotype with monotypic surface Lambda immunoglobulin light chain expression. Immunohistochemical studies performed on tissue section revealed that neoplastic lymphoid nodules were positive for CD20, BCL-6 and BCL-2 with low proliferation index by Ki-67 staining, but again negative for CD45. FISH analysis confirmed the presence of IGH/BCL2 rearrangement. Follicular lymphoma (grade 1) with negative CD45 expression was diagnosed. Conclusions: Loss of CD45 expression has been reported, but all cases documented were large B cell lymphoma (LBCL). To the best of our knowledge, this is the first case of low grade follicular lymphoma with lack of CD45 expression. We report this case because of not only its rarity but also to raise awareness that loss of CD45 expression can occur in LBCL as well as in low grade B-cell lymphomas. © 2015 Elsevier B.V..


Zhu Y.,Nantong University | Cao X.,Nantong University | Xu D.,Cblpath Inc.
Laboratory Hematology | Year: 2011

The Beckman Coulter LH 750 with VCS technology can determine intrinsic biophysical properties of peripheral leukocytes in their "near native state." We investigated the possibility of the LH 750 to detect morphologic changes in lymphocytes/monocytes in hepatitis B virus (HBV) infection. The VCS data from 37 active HBV patients, 140 HBV carriers, and 655 controls were analyzed. We observed significant increases in lymphocyte volume (LV) and the lymphocyte volume SD (LV-SD), and a significant decrease in lymphocyte conductivity (LC) in HBV infection, compared with controls. Similar changes in monocyte volume and conductivity were also observed. A simplified parameter, the Lymph-Index (calculated as [LV LV-SD]/ LC]) is proposed. The Lymph-Index was significantly increased in HBV carriers compared with controls (13.86 ± 2.73 versus 11.83 ± 1.36; P < .001). The increase was more pronounced in patients with active HBV infection (18.84 ± 3.88 versus 11.83 ± 1.36; P < .0001). With a cutoff value of 18.2 for the Lymph-Index, a 91.7% sensitivity and a 95.7% specificity were achieved in diagnosing active HBV infection. This overall sensitivity and specificity were superior to those of the individual lymphocyte/monocyte VCS parameters. In conclusion, VCS parameters can reflect the morphologic changes of lymphocytes/monocytes in HBV infection and may be useful clinically to aid in identifying HBV infection in a population with a high HBV prevalence. © 2011 Carden Jennings Publishing Co., Ltd.


Tang H.,Zhejiang University | Jing J.,Zhejiang University | Bo D.,Jiangsu University | Xu D.,Cblpath Inc.
Archives of Pathology and Laboratory Medicine | Year: 2012

Context.-The Coulter DxH 800 hematology analyzer can determine leukocyte numerical parameters (total leukocyte counts and differentials). It also measures intrinsic biophysical properties of these cells in their near-native state. These morphologic measurements are known as cell population data (CPD). Objective.-To study, for the first time, the biological variations of morphologic parameters or CPD and reinvestigate numerical parameters using the newest Coulter hematology analyzer. Design.-Forty adult volunteers (21 women, 19 men) were included. All participants maintained their normal lifestyles. Blood samples were drawn in duplicate by a single experienced phlebotomist and analyzed within 2 hours using a single analyzer. Before each batch analysis, the instrument quality controls were performed using the same lots of reagents. Results.-Within-subject (CVI) and between-subjects (CVG) biological variations for numerical parameters are smaller than previously reported. Cell population data have much smaller overall CVI and CVG compared to numerical parameters, suggesting that these parameters are less variable around the homeostatic set point intraindividually and interindividually. Index of individuality (ratio of CVI/CVG) for CPD was low. In addition, intraday and interday biological variations of all parameters are fairly constant. Conclusions.-These observations are clinically valuable. Data on CVI and analytical precision may be used to generate objective delta-check values for use in quality management. Comparing CVI and CV G on CPD may allow us to decide the utility of traditional population-based reference ranges. Documentation of CPD on biological variations is an essential prerequisite in the development of any new application clinically.


Zhang P.,Zhejiang University | Tang H.,Zhejiang University | Chen K.,Zhejiang University | Chen Y.,Jiangsu University | Xu D.,Cblpath Inc.
Archives of Pathology and Laboratory Medicine | Year: 2013

Context.-The Coulter DxH 800 hematology analyzer can determine conventional hematologic parameters. It also provides many new hematologic parameters, some of which show potential clinical utility. Objective.-To study, for the first time, the biological variations of new hematologic parameters and reinvestigate the biological variations of conventional hematologic parameters using the newest Coulter hematology analyzer. Design.-Forty adult volunteers (21 women and 19 men) were included. All participants maintained their normal lifestyles. Blood samples were drawn in duplicate by a single experienced phlebotomist and analyzed within 2 hours using a single analyzer. Before each batch analysis, the instrument quality controls were performed using the same lots of reagents. Results.-Within-subject and between-subject biological variations for the conventional hematologic parameters were compatible with published data. The analytic variation of the DxH 800 for these parameters appeared smaller. Index of individuality (ratio of within-subject to between-subject biological variation) for all parameters was low. In addition, intraday and interday biological variations of most parameters studied are fairly constant among the population examined. Conclusions.-These observations are clinically valuable. Data on within-subject biological variation and analytic precision may be used to generate objective delta-check values for use in quality management. Comparing within-subject and between-subject biological variation on new parameters may allow us to decide the utility of traditional population-based reference ranges. Furthermore, documentation of biological variations of new parameters is an essential prerequisite in the development of any clinical application in the future. Copyright © 2013 College of American Pathologists.


Zhu Y.,Nantong University | Cao X.,Nantong University | Chen Y.,Nantong University | Zhang K.,Nantong University | And 3 more authors.
International Journal of Laboratory Hematology | Year: 2012

Introduction: The Coulter LH750 analyzer can determine white blood cell count (WBC) with differentials and determine their intrinsic biophysical properties, known as cell population data (CPD). Previous studies demonstrated that neutrophil CPD, mean neutrophil volume (MNV), and neutrophil volume distribution width (NDW) were significantly increased during bacterial infection. This study investigated the clinical usefulness of MNV/NDW to differentiate postoperative infection. Methods: Cell population data from 212 controls, 135 patients without postsurgical infection, and 62 with infection were studied. All patients had normal WBC and percent neutrophils prior to surgery. Results: No significant differences in MNV and NDW were observed before surgery between infected and noninfected patients when compared with controls. The MNV and NDW in infected patients were significantly increased after surgery when compared with noninfected patients. Although WBC and percent neutrophils were also increased after surgery, no statistical differences were seen between noninfected and infected patients. A MNV cutoff of ≥152.4 and NDW cutoff of ≥24.26 produced 90.3% and 88.3% sensitivity and 88.4% and 76.3% specificity, respectively. Conclusion: Neutrophil CPD, MNV, and NDW are better indicators for postoperative bacterial infection. The potential clinical usefulness of these parameters merits further exploration in a larger prospective study. © 2011 Blackwell Publishing Ltd.


Niu X.,Southern Medical University | Li S.,Southern Medical University | Wei F.,Southern Medical University | Huang J.,Southern Medical University | And 4 more authors.
Breast Cancer | Year: 2014

Objective: Apogossypolone (ApoG2), a new derivative of gossypol, is a potent cell-growth inhibitor. ApoG2 has been demonstrated to have superior anti-tumor activity than gossypol in Bcl-2 transgenic mice. The purpose of this study was to investigate the inhibitory effect of ApoG2 on breast cancer cell line MCF-7 in vitro and in vivo, and to investigate its anti-tumor mechanism. Methods: MCF-7 cell line in culture was treated with ApoG2. The inhibitory effects of ApoG2 on cell growth were measured by MTT and colony-formation assay. The cell apoptotic rate and cell cycle were analyzed by use of flow cytometry (FCM). The ultrastructural changes were observed by transmission electron microscopy. Autophagy was detected by acridine orange staining. Expression of Bcl-2, Bax, and Beclin 1 proteins was measured by western blot analysis. Results: The inhibitory effect of ApoG2 on MCF-7 cell proliferation was dose and time-dependent. The maximum effect was observed when cells were incubated for 72 h with 40 μM ApoG2. ApoG2 at 5 μM also inhibited colony formation. FCM assay indicated that ApoG2 induced cell apoptosis and caused cell arrest in the S phase and G2/M phase. Transmission electron microscopic examination and acridine orange staining showed that ApoG2 induced intracellular autolysosome formation. Furthermore, ApoG2 reduced Bcl-2 expression, and enhanced expression of Bax and Beclin 1. Xenografting of MCF-7 cells in mice can also be inhibited by ApoG2. Conclusion: ApoG2, a novel anti-apoptotic Bcl-2 agent, inhibits proliferation of breast cancer cell line MCF-7 by inducing cell apoptosis and autophagy. © 2012 The Japanese Breast Cancer Society.


Zhu Y.,Nantong University | Cao X.,Nantong University | Tao G.,Nantong University | Xie W.,Nantong University | And 2 more authors.
International Journal of Infectious Diseases | Year: 2013

Objective: An LH750 hematology analyzer with VCS (volume, conductivity, and light scatter) technology can determine morphologic properties of peripheral leukocytes, known as cell population data (CPD). We have previously demonstrated that the lymphocyte CPD exhibit significant changes in acute hepatitis B virus infection. A simplified lymphocyte CPD, the lymph index, was proposed. We conducted the current study to further evaluate the clinical usefulness of the lymph index, and included patients with various viral infections, as well as those with acute bacterial infections. Methods: Peripheral blood was collected from 72 patients with viral infections, 46 patients with acute bacterial infections, and 204 controls. The lymphocyte CPD included the mean volume (LV) with its standard deviation (LV-SD) and the conductivity (LC). The lymph index was calculated as LV. ×. LV-SD ÷ LC. Results: The lymph index was significantly increased in viral infections and only mildly increased in acute bacterial infections compared to controls. Using a lymph index cutoff value of ≥12.92, we achieved 91.67% sensitivity and 97.2% specificity for diagnosing viral infection. Conclusions: The findings may be clinically useful since these morphological parameters are readily obtained by hematology analyzer during automated leukocyte differentials. They are quantitative, objective, and fast. The lymph index could be a potential hematological parameter for viral infection. © 2012 International Society for Infectious Diseases.


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