Revilla E.,Autonomous University of Madrid |
Carrasco D.,CBGP INIA |
Benito A.,Instituto Madrileno Of Investigaciony Desarrollo Rural |
Arroyo-Garcia R.,CBGP INIA
American Journal of Enology and Viticulture | Year: 2010
This study investigated the anthocyanin composition of 21 mostly Spanish wild grapevine accessions preserved at El Encín Germoplasm Bank and selected in consideration of observed ampelographic differences and molecular characterization. Sampling was carried out in 2006, 2007, and 2008. After extraction from grape skins, total anthocyanins was determined by spectrophotometry and the anthocyanin fingerprint of grapes, based on 15 anthocyanin variables, was determined by HPLC. Total anthocyanin concentration was similar to that found in winegrape cultivars. The accessions studied showed considerable variability in their anthocyanin fingerprints and it was possible to distinguish several groups, similar to previous reports on the anthocyanin fingerprint of winegrapes. The anthocyanin composition of wild grapevine accessions was similar to that of cultivated grapes. Nevertheless, the presence of wild accessions with anthocyanin fingerprints uncommon or nonexistent in Spanish cultivated varieties suggests that the genetic variability related to anthocyanins in Spanish wild grapevine populations may be higher than that of cultivated varieties commonly considered of Spanish origin. © 2010 by the American Society for Enology and Viticulture. All rights reserved.
De AndrEs M.T.,IMIDRA |
Benito A.,IMIDRA |
PErez-Rivera G.,CBGP INIA |
Ocete R.,University of Seville |
And 6 more authors.
Molecular Ecology | Year: 2012
The wild grapevine, Vitis vinifera L. ssp. sylvestris (Gmelin) Hegi, considered as the ancestor of the cultivated grapevine, is native from Eurasia. In Spain, natural populations of V. vinifera ssp. sylvestris can still be found along river banks. In this work, we have performed a wide search of wild grapevine populations in Spain and characterized the amount and distribution of their genetic diversity using 25 nuclear SSR loci. We have also analysed the possible coexistence in the natural habitat of wild grapevines with naturalized grapevine cultivars and rootstocks. In this way, phenotypic and genetic analyses identified 19% of the collected samples as derived from cultivated genotypes, being either naturalized cultivars or hybrid genotypes derived from spontaneous crosses between wild and cultivated grapevines. The genetic diversity of wild grapevine populations was similar than that observed in the cultivated group. The molecular analysis showed that cultivated germplasm and wild germplasm are genetically divergent with low level of introgression. Using a model-based approach implemented in the software structure, we identified four genetic groups, with two of them fundamentally represented among cultivated genotypes and two among wild accessions. The analyses of genetic relationships between wild and cultivated grapevines could suggest a genetic contribution of wild accessions from Spain to current Western cultivars. © 2011 Blackwell Publishing Ltd.