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Jaboticabal, Brazil
Jaboticabal, Brazil
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Ranzani-Paiva M.J.T.,Institute Pesca Of Sao Paulo | Lombardi J.V.,Institute Pesca Of Sao Paulo | Maiorino F.C.,Laboratory and Vet Diagnostico e Consultoria Ltda | Goncalves A.,CAUNESP | Dias D.C.,Institute Pesca Of Sao Paulo
Boletim do Instituto de Pesca | Year: 2014

The aim of this study was to analyze the effects of chronic toxicity of sodium selenite (Na2SeO3 SE4+) on juvenile Nile tilapia Oreochromis niloticus. Therefore, a toxicity test was carried out with three sub-lethal concentrations of that salt (0.4 mgSe L-1, 0.04 mgSe L-1 and 0.01 mgSe L-1), plus a control group. The experiment was carried out for 14 days, sampling six individuals per treatment in intervals of 0, 3, 7, 10 and 14 days. Effects of chronic sublethal concentrations of selenite were evaluated by routine haematological and histopathological analysis. There were significant differences (P<0.05) in the rate of hemoglobin, mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) and an increase of the total number of leukocytes, mainly due to the increased number of lymphocytes, monocytes, and neutrophils. Severe gill hyperplasia was found on the fourteenth day. Nephrosis were found in the cephalic kidney, characterized by glomerulonephritis and tubular vacuolar degeneration, which was a result of the necrosis or secondary infection, glomerular atrophy and glomerulosclerosis, proliferative glomerulonephritis, nephrosis, nephrosclerosis, tubular calcification, edema and hemorrhage. It was found that selenite, at the tested concentrations despite being sublethal, caused histological and hematological changes in Nile tilapia.


de Oliveira Filho P.R.C.,CAUNESP | Favaro-Trindade C.S.,USP FZEA | Trindade M.A.,USP FZEA | Balieiro J.C.C.,USP FZEA | And 2 more authors.
Scientia Agricola | Year: 2010

Filleting yield of Nile tilapia Oreochromis niloticus (L.) is low (30%) and generates large amount of wastes that may turn into environmental and economic problem. However, these wastes can be used for the extraction of minced fish (MF) which can be used in the preparation of sausages. The objective of this study was to assess the quality of sausages prepared with 0, 20, 40, 60, 80 and 100% of MF from Nile tilapia filleting waste during storage at 0±0.3°C. Alterations in the instrumental color (L*, a* and b*), lipid oxidation (TBARS), total volatile nitrogenous bases (TVB-N), pH, microbiological condition (pathogenic bacteria and aerobic psychrotrophic bacteria), and sensory attributes (color, odor, flavor, texture and overall acceptability) were evaluated for up to 40 days. The addition of MF to sausages increased TBARS values and decreases TVB- N, L*, a* and b* values. Acceptability of color attribute decreased with increasing MF; best flavor, texture and overall acceptability scores were registered for sausages containing 40 and 60% MF; best odor was registered for 100% MF. Pathogenic microorganisms were not detected, but decrease in pH and proliferation of aerobic psychrotrophic bacteria which, however, did not compromise sensory evaluation of sausages were registered throughout storage. Sausages prepared with MF from tilapia filleting waste have a shelf-life of 40 days when stored at 0±0.3°C, and the maximum recommended MF inclusion to maintain good sensory quality is 60%.


Romagosa E.,Institute Pesca | Souza B.E.,CAUNESP | Sanches E.A.,CAUNESP | Baggio D.M.,University of Toledo | Bombardelli R.A.,University of Toledo
Journal of Applied Ichthyology | Year: 2010

The objective of this study was to assess the effects of an activating solution on the sperm motility duration (SMD) of 'curimbatá', Prochilodus lineatus through of the definition of qualitative and quantitative parameters of the semen pool used in the experiment; evaluation of the effects of different ratios of semen dilution corresponding to 1-:-1, 1-:-2, 1-:-20, 1-:-200, 1-:-2000, 1-:-20-000 and 1-:-100-000 semen:dilute solution on the SMD and, assessment of the effects of different temperatures of the activating solution (5, 10, 15, 20, 25, 30, 35, 40, 45 and 50°C) on the SMD. The results of SMD were directly proportional to the dilution (P<0.05), starting from the dilution of 1-:-2 (semen:water), with 23.04-s. Were used three replicates of the semen pool for each test. Two-year-old brookstock were maintained in ponds culture conditions. In November-December 2006, twelve mature males broodfish were selected (mean weight and length of 405.8±134.2-g and 25.6±3.1-cm, respectively). The males released that semen under slight pressure of the urogenital papilla were selected for the experiment. The SMD increased proportionally to the increase in dilution, until it reached a maximum of 28.83-s for the ratio 1-:-100-000 semen: dilute solution. The results of SMD in relation to the temperature of the activating solution exhibited a quadratic behavior (P<0.05) with a maximum theoretical performance in terms of sperm motility duration of 21.36-s at a temperature of 17.3°C. Thus, for the species considered, the increase in the dilution ratio proved favorable for the rise in motility duration until the maximum value studied of 1-:-100-000 semen:dilute solution. As for the temperature of the activating solution, the best results of SMD were obtained at the temperature of 17.3°C. At higher temperatures used in the experiment (25, 30, 35, 40, 45 and 50°C), a decrease in motility duration. © 2010 Blackwell Verlag, Berlin.


de Negreiros Sousa S.M.,West Parana State University | Freccia A.,West Parana State University | dos Santos D.L.,Federal University of Paraná | Meurer F.,Federal University of Paraná | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2013

This study was carried out to evaluate the influenceofdifferentlevelsofdigestibleenergyanddigestibleprotein in diets of Nile tilapia (Oreochromis niloticus) broodstock on offspring growth during sex reversal stage. A total of 2,700 post-larvae (8.2±0.001 mg) derived from breeders fed nine diets with distinct levels of digestible protein (28, 34 and 40% DP) and digestible energy (2,800; 3,400; and 4,000 kcal DE.kg-1) were stored in twenty-seven 70 L tanks. After 30 days of growth, their average finalweight(AFW,g),averageweightgain(AWG,g),finalstandardlength(SL,cm),conditionfactor(CF), specificgrowthrate(SGR,%/day),stockuniformity(UNI,%),survival(SUR,%)andsexreversalrate(SRR,%)weremeasured. Although female nutrition is thought to influencetheperformanceorqualityofprogenyduringearlylifestages,noinfluenceofdietssuppliedtobroodstockwasdetectedonanyparameter.Nonetheless,theoffspringpresentedcommerciallysatisfactory growth rates. Thus, diets containing 28% of digestible protein and 2,800 kcal of digestible energy.kg of diet-1 can be used to feed Nile tilapia broodstock without jeopardizing offspring performance during the sex reversal phase ©2013 Sociedade Brasileira de Zootecnia.


de Carvalho P.L.P.F.,São Paulo State University | da Silva R.L.,CAUNESP | Botelho R.M.,São Paulo State University | Damasceno F.M.,São Paulo State University | And 2 more authors.
Boletim do Instituto de Pesca | Year: 2012

The aim was to evaluate the nutritional value of the root and leaves of cassava (Manihot sculenta) for juveniles of Nile tilapia (Oreochromis niloticus). Three diets were prepared, a reference and two test diets. The first tested diet contained 50.0% of cassava root and 50.0% of reference diet and the second one consisted of 30.0% of cassava leaf meal and 70.0% of reference diet. Moreover, in every diet was added 0.1% chromium oxide-III (Cr2O3) as inert marker. Sixty juveniles (100.0 ± 9.0 g) were accommodated in cages (10 fish/cage) for feeding and feces collection. During the day these cages were maintained in a feeding tank (250 L), one tank for each treatment, where they were fed until apparent satiation. After the aforementioned feeding period they were transferred to a fecal collection tank, where they remained until next morning. The collected feces were frozen and dried for analyzes. The apparent digestibility coefficients (ADC) were calculated on basis of Cr2O3 concentration present in diets and feces. The ADC of reference diet for DM, cassava leaf and root were, respectively 85.23%, 81.22% and 91.44%; CP to 97.35%, 73.37% and 91.46% and for the GE to 87.70%, 64.70% and 92.20%, the values obtained for the diet with cassava root significantly higher (P<0.05). Summarizing, root and cassava leaves can be used as alternative ingredients for compose balanced diets for Nile tilapia juveniles.


Lui T.A.,West Parana State University | Bittencourt F.,CAUNESP | Neu D.H.,West Parana State University | Dallagnol J.M.,West Parana State University | And 2 more authors.
Archivos de Zootecnia | Year: 2012

The aim of this study was to evaluate the effects of fish oil acidity on the growth of pacu (Piaractus mesopotamicus) juveniles. Two hundred juveniles with 10.53±0.65 g of weight and 8.74±0.13 cm of total length, were distributed in a totally randomized design in 20, 0.15 m3 meshmade tanks placed in a 25 m3 holding tank. Extruded commercial diets sprayed with soybean oil (control) with acidity index of 0.24 and fish oil with acidity index of 1.48, 6.40 and 9.85 were used. The fish were fed four times a day until apparent satiety. Productive performance, centesimal composition and blood biochemistry were analysed. At the end of the feeding period none of the analyzed parameters was affected by the use of acidified fish oil. Therefore, fish oil may be supplied to the pacu juveniles regardless of their pH.


Zanini H.L.H.T.,São Paulo State University | do Amaral L.A.,São Paulo State University | Zanini J.R.,São Paulo State University | Tavares L.H.S.,CAUNESP
Engenharia Agricola | Year: 2010

The evaluation of water quality index (WQI) and mean trophic state index (mTSI) may be useful for management and administration projects of water systems. Quality of water from the stream Rico micro-basin that supplies the town of Jaboticabal - SP, Brazil, with fresh water has been evaluated, using WQI and mTSI. Collects were undertaken between September 2007 and August 2008, at three sites: a) one of the sources, b) after the Sewage Treatment Stations of Monte Alto city, and c) in the abstraction of water for public supply of Jaboticabal. It was analyzed the physical, chemical and microbiological variables of the water: temperature, pH, BOD5, total nitrogen, total phosphorous, turbidity, total residue, dissolved oxygen, orthophosphate and chlorophyll-a. According to the results, it was concluded: (a) the anthropic activities developed in the neighborhood of the Rico Stream reduces the quality of the water during different times of the year; (b) the average values of WQI in three points analyzed showed a direct relationship with the average values of mTSI, but there was more discrimination of water quality by mTSI, identifying different trophic degrees for points and periods of sampling; (c) the WQI showed better differentiation of water quality in dry period and the mTSI showed better differentiations in the rainy season; (d) the natural depuration and the confluence of the Tijuco Stream with the Rico Stream contribute to better water quality, making it suitable for urban supply after conventional treatment.


Pinheiro S.R.F.,São Paulo State University | Sakomura N.K.,São Paulo State University | Nascimento D.C.N.,São Paulo State University | Dourado L.R.B.,São Paulo State University | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2011

It was performed three trials to determine the nutritional levels of available phosphorus (aP) for males and females of the strain ISA Label in the starter (1 to 28 days), growing (28 to 56 days) and finisher (56 to 84 days) phases, reared in a free-range system. In each trial, 480 birds with age corresponding to rearing phase were housed in 24 experimental units with shelter and pasture areas. It was used a complete random experimental design, in a 4 × 2 factorial scheme (levels of aP and sexes) with three replicates of 20 birds. The levels of aP evaluated were the following: 0.25, 0.36, 0.47 and 0.58% in the starter phase; 0.18, 0.31, 0.44 and 0.57% in the growing phase; and 0.14, 0.27, 0.40 and 0.53% in the finisher phase. Body weight gain, feed intake, aP intake, feed conversion, contents of phosphorus, calcium and ash in the tibia and bone breaking strength were evaluated. According to results, the optimum dietary aP levels in the starter phase, for males and females, are 0.39 and 0.49%, which correspond to the intake of 3.94 and 3.96 g of aP/bird, respectively. For the growing phase, it is recommended 0.35% of aP in the diet for birds of both sexes, which correspond to an intake of 8.45 and 6.70 g of aP/bird. In the finisher phase, it is recommended the levels 0.32 and 0.30% of aP, which corresponds to intakes of 12 g and 9.5 g aP/bird for males and females, respectively. © 2011 Sociedade Brasileira de Zootecnia.


Pinheiro S.R.F.,São Paulo State University | Sakomura N.K.,São Paulo State University | Kawauchi I.M.,São Paulo State University | Bonato M.A.,São Paulo State University | And 2 more authors.
Revista Brasileira de Zootecnia | Year: 2011

The objective of this work was to determine inclusion levels of sodium chloride (NaCl) in diets for male and female Colonial strain birds in the starter (from one to 28 days of age), growing (from 28 to 56 days of age) and finisher (from 56 to 84 days of age) phases reared in free-range system. In each trial, 480 birds at age corresponding to the rearing phase were housed in 24 experimental units containing areas of shelter and pasture. The experimental design used was a completely randomized in a 4 × 2 factorial arrangement (NaCl levels × sex), totaling eight treatments and three replications of 20 birds. Levels of NaCl evaluated were: 0.20, 0.40, 0.60 and 0.80% in the starter phase; 0.10, 0.30, 0.50 and 0.70% in the growing phase and 0.10, 0.25, 0.40 and 0.55% in the finisher phase. It was evaluated weight gain, feed intake, water intake, feed conversion, pH, partial pressure of carbon dioxide in the blood, hematocrit concentration and sodium and chlorine blood levels. For the starter phase it is recommended 0.53% of NaCl level in diet for birds of both sexes. For the growing phase, the levels were 0.40 and 0.43% of NaCl, respectively, for males and females, and for the finisher phase, the level of 0.25% for birds of both sexes meets the nutritional requirements. © 2011 Sociedade Brasileira de Zootecnia.


Fogaca F.H.,Embrapa Meio Norte | Sant'Ana L.S.,CAUNESP | Lara J.A.F.,Embrapa Pantanal | Mai A.C.G.,Embrapa Meio Norte | Carneiro D.J.,CAUNESP
Food and Bioproducts Processing | Year: 2015

The tilapia fileting industry generates large amounts of nutritionally significant waste material, and the recovery of this material is important. The manufacture of restructured products from mechanically recovered fish meat (MRFM) obtained from tilapia fileting allows the use of proteins of high biological value that would otherwise be discharged into the environment. The objective of this study was to evaluate the effect of washing cycles (either one cycle or five cycles) and of the addition of tapioca starch (20% vs. a no-starch control) on the characteristics of surimi obtained from MRFM produced by the tilapia industry and destined for use in restructured products. To evaluate the quality attributes of the product, the structure of a surimi protein matrix and its relationship to selected physicochemical parameters and morphological characteristics was assessed. Both the number of washing cycles and the starch addition were found to influence the moisture, protein and lipid content of the MRFM surimi. Higher whiteness was found after five washing cycles. Because the tapioca starch acted as a stabilizer, the fat globules were more stable and well distributed, and an emulsion with better properties resulted. A homogeneous network of fat globules linked to the protein matrix by a layer of tapioca starch was formed. Another advantage of this approach is that tapioca starch is gluten free. This property is important for specific groups in the population, e.g., celiac-intolerant consumers. © 2014 The Institution of Chemical Engineers. Published by Elsevier B.V. All rights reserved.

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