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Zhang N.,CAU BUA TCVM Teaching and Research Team | Zhang N.,Key Laboratory of Development and Evaluation of the Chemical and Herbal Drugs for Animal Use | Guo S.,South China Agricultural University | Li H.,China Agricultural University | And 14 more authors.
Journal of Animal and Veterinary Advances | Year: 2012

Sijunzi decoction (SJZ), composed of Dangshen (Codonopsis pilosula Nannf.), Baizhu (Atractylodes macrocephala Koidz.), Filling (Tuckahoe) and Gancao (Radix glycyrrhiza) is well known as a classical Chinese traditional and herbal medicamentum aiming directly at spleen deficiency in Traditional Chinese Medicine (TCM). The present research is to evaluate the regulatory effect of SJZ on small intestinal T lymphocyte subsets differentiation in reserpine-induced spleen deficiency rats. Reserpine induced spleen deficiency rats were orally administrated with SJZ (0.2 mL/200 g, 0.6 mL/200 g and 1.0 mL/200 g, body weight) once a day for a period of 5 days. Clinical symptoms including body weight were observed every day, the small intestine damage and recovery by SJZ were detected by electron microscope scanning, small intestine lymphocytes were separated for T lymphocyte subsets detection by flow cytometry and T lymphocyte related cytokine expression was studied using quantitative real time PCR. Results showed that proper dose of SJZ can significantly promote the recovery of body weight and small intestine damage as well as regulate the differentiation of CD3 +, CD3 +CD4 + T lymphocyte subsets and the expression of Type 1 cytokines (IL-2, IFN-γ and IL10). Besides, a significant CD4 -CD8 - T lymphocyte subset was found in reserpine treated and high dose SJZ administrated rats. Data from this study proved that SJZ possess a significant promoting effect on the amelioration of spleen deficiency, CD4 + T lymphocyte and cytokines related with regulation of T lymphocyte differentiation would be key targets of SJZ on spleen deficiency. © Medwell Journals, 2012.


Guo K.-J.,China Agricultural University | Guo K.-J.,CAU BUA TCVM Teaching and Research Team | Liu F.-H.,China Agricultural University | Liu F.-H.,CAU BUA TCVM Teaching and Research Team | And 4 more authors.
Agricultural Sciences in China | Year: 2010

The aims of the study were to evaluate productive performances and environmental impact of double-muscled Piemontese culled cows. In two trials, 39 Piemontese culled cows aged from 46 to 176 mon were fattened using diets commonly applied in farm conditions. The animals were weighed at the beginning and end of the trials. Concentrate and hay intake were recorded daily. After slaughtering, meat, fat and bone yields were recorded and dressing percentage was calculated. The backward linear regression model was employed for statistical analysis. Results showed average body weight (ABW) of cows as (556.97 ± 55.99) kg and relatively low dry matter intake (DMI) [(9.17 ± 0.35) kg d -1] during the fattening period. The ratio between DMI and ABW, i.e., ratio between intake and weight (RIW), (1.66 ± 0.18)% was negatively influenced by initial body weight (IBW) and duration of finishing period (D), but age had no effect on RIW. Average daily gain (ADG) was relatively low [(0.55 ± 0.21) kg d -1], and was negatively correlated with IBW and D, which implied that lower IBW and shorter D could have higher ADG and vice versa. The dressing percentage (DP) was (59.80 ± 1.83)% and the carcass red meat yield (MY) was (75.17 ± 3.10)%. Nevertheless, N efficiency was very low [(4.35 ± 1.76)%], which must be considered in relation to environmental protection so both animal requirements and a well balanced diet need more attention. In conclusion when Piemontese cows will be culled and fattened, IBW and D should be considered carefully in order to get a reasonable ADG and N efficiency to guarantee productive benefits and environmental protection at the same time. © 2010 Chinese Academy of Agricultural Sciences.


Wang N.,China Agricultural University | Cheng G.,China Agricultural University | Guo K.,China Agricultural University | Liu F.,China Agricultural University | And 3 more authors.
2011 International Conference on Remote Sensing, Environment and Transportation Engineering, RSETE 2011 - Proceedings | Year: 2011

E. colibacillosis is considered to be one of the major bacterial diseases in the poultry industry. In this study, E. colibacillosis was induced by artificial infection in chicken and cured by Changliqing decoction, thus to verify the therapeutic effect via clinical trials. Several factors, including clinical symptoms, mortality rate, hatching performance, autopsy changes, were monitored and analyzed. Data showed that compared with positive control, the mortality rate were dramatically lower, indicating a significance of difference (p<0.05), but are dosage independent. Furthermore, high and medium dosage groups of Changliqing decoction treated chicken showed remarkably higher rate on daily gain against both positive control and positive drug control (p<0.05). Additionally, by analyzing the clinical symptoms and autopsy changes, there is no significant difference (P>0.05) between the control group and Changliqing decoction treated groups (dosage irrelevant). All above results indicates that Changliqing decoction treatment potentially performs positive for curing E. colibacillosis on clinical level and recommended dosage is about 1.5 ml/kg once a day. © 2011 IEEE.


Lu A.,China Agricultural University | Wang H.,China Agricultural University | Hou X.,China Agricultural University | Li H.,China Agricultural University | And 9 more authors.
Journal of Biomolecular Screening | Year: 2011

Ambient temperature is a critical factor that affects biological organisms in many ways. In this study, the authors investigated gene expression changes in rat small intestine in response to heat stress. Male Sprague-Dawley rats were randomly divided into control and heat-stressed groups. Both groups were housed at 25 °C, although the heat-stressed group was also subjected to 40 °C for 2 h each day for 10 successive days. Rats were sacrificed 1, 3, 6, and 10 days after heat treatment, and sections of their small intestine epithelial tissue were excised for morphological examination and microarray analyses. The rat rectal and body surface temperatures and serum cortisol levels were all significantly increased after heat treatment (p < 0.05). The jejuna were significantly damaged by 3 days after heat treatment began. Microarray analysis showed that 422 genes were differentially expressed, of which 290 genes were significantly upregulated and 132 genes were significantly downregulated. Subsequent bioinformatics analyses revealed that the differentially expressed genes were mainly related to stress, immune regulation, and metabolism processes. The bioinformatics analysis of the differentially expressed genes should be beneficial to further investigations on the underlying mechanisms involved in heat stress-induced damage in the small intestine. © 2011 Society for Laboratory Automation and Screening.

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