Hammouche S.B.,University of Science and Technology Houari Boumediene |
Remana S.,University of Science and Technology Houari Boumediene |
Exbrayat J.-M.,Catholic University of Lyon
Comptes Rendus - Biologies | Year: 2012
The hormonal regulation of hepatic synthesis of vitellogenin during the annual reproductive cycle was performed for the first time in the deserticole, oviparous, diurnal and herbivorous Uromastyx acanthinura, a lizard belonging to the Agamidae family. In order to elucidate what kind of estrogen receptor is involved in this process, an immunohistochemical study was performed. Changes were obtained in the labeling and cellular distribution of the estrogen and progesterone receptors according to the period of the reproductive cycle and the experimental administration of 17β-estradiol. Only the ERβ subtype was present; it was found in all phases of the cycle with a variable localization: nuclear and cytosolic during vitellogenesis, mainly cytosolic in the female with egg retention (luteal phase) and strictly cytosolic in females at sexual rest. The progesterone receptors were present only at the luteal phase and during sexual rest and disappeared completely from females after 17β-estradiol treatment in sexual rest. Our data suggested that mediation of action of the 17β-estradiol in the vitellogenin synthesis in the lizard U. acanthinura occured via ERβ. PRA and PRB could both be necessary for the negative effect of progesterone on the hepatic synthesis of vitellogenin. © 2012 Académie des sciences. Published by Elsevier Masson SAS. All rights reserved.
Seitz H.,German Cancer Research Center |
Dantheny T.,Catholic University of Lyon |
Dantheny T.,University of Strasbourg |
Burkart F.,German Cancer Research Center |
And 2 more authors.
Clinical and Vaccine Immunology | Year: 2013
Current commercial prophylactic human papillomavirus (HPV) vaccines are based on virus-like particles assembled from the major capsid protein L1 and show excellent safety and efficacy profiles. Still, a major limitation is their rather narrow range of protection against different HPV types. In contrast, the minor capsid protein L2 contains a so-called major cross-neutralizing epitope that can induce broad-range protective responses against multiple HPV types. This epitope is conserved among different papillomaviruses (PV) and contains two cysteine residues that are present in the L2 proteins of all known PV types. The main challenge in developing L2-directed vaccines is to overcome the intrinsically low immunogenicity of the L2 protein. Previously, we developed a recombinant L2-based prototype vaccine by inserting peptide epitopes spanning the cross-neutralizing L2 sequence into a bacterial thioredoxin (Trx) scaffold. These antigens induced high-titer neutralizing antibodies in mice. Here, we address the question of whether Trx scaffold multimerization may further enhance the immunogenicity of the TrxL2 vaccine. We also demonstrate that the oxidation state of the conserved cysteine residues is not essential for vaccine functionality, but it contributes to immunogenicity. Copyright © 2013, American Society for Microbiology. All Rights Reserved.
Plouffe S.,University of Montréal |
Lanoie P.,HEC Montréal |
Berneman C.,ESC Saint Etienne |
Vernier M.-F.,Catholic University of Lyon
Journal of Cleaner Production | Year: 2011
This paper reports results of an empirical study conducted to assess the profitability of ecodesigned products. Indeed, the advantages related to ecodesign are numerous according to the literature on the subject. Not only is it considered to be an efficient environmental approach, it is also said to carry a number of economic advantages for the firms adopting it. Among these advantages, the potential reduction of costs and an increase in revenues are the most important drivers. Nevertheless, the economic benefits have not been demonstrated, only indicated as a potential outcome. The main purpose of the study was to assess the economic impact of ecodesigned products and to compare the results of French companies with those of Quebec. Semi-structured interviews were conducted with 30 firms on their related ecodesign experience (15 in France, 15 in Quebec), the subsequent results were reached pertaining to the economic benefits tied to such an approach. In 24 cases, firms increased their profits with ecodesigned products. In three cases, profits are equivalent and in the three remaining cases, it is not obvious that the increased revenues compensate for the reduction of margin. French companies were found to be more profitable than the Quebec cases; this can be explained by the fact that French companies have a more systemic approach and therefore manage to make cost savings at several stages in the product life-cycle. We can conclude that the most important aspects to consider when getting involved in an ecodesign project is to focus on increased functionality of the product and to seek environmental and economic improvement on as many life-cycle stages as possible. © 2010 Elsevier Ltd. All rights reserved.
Merucci M.,Catholic University of Lyon
Psychotherapies | Year: 2012
In this article the authors (a psychologist and a psychomotrician, both women) relate a group experience for preadolescent and adolescent polyhandicapped girls with language impairment. The working hypotheses were based on H. Wallon's theories on emotions. Empathy is seen as having structuring elements in social communication. In the context of a mediation group these girls were able to recognize themselves as being sexed and partaining to time.
Denizeau L.,Catholic University of Lyon
Douleurs | Year: 2012
The IASP definition describes pain as sensation and emotion. From an anthropological point of view, these two dimensions do not exhaust the whole problem of pain's personal experience. This paper suggests an approach of pain as a perception, to highlight the meaning registers in chronic pain experience, which cannot be reduced to individual meanings. So, it underlines the stake in the treatment of pain not only to focus on its relief but also to work on these representations, and thus on relations between the person and its pain. © 2012.
Moudilou E.N.,Catholic University of Lyon |
Mouterfi N.,Catholic University of Lyon |
Exbrayat J.-M.,Catholic University of Lyon |
Brun C.,Catholic University of Lyon
Tissue and Cell | Year: 2010
Calpains are cytoplasmic proteases activated by calcium, implicated in cell differentiation and apoptosis. The best characterized enzymes are calpains 1-3. The aim of this work was to localize calpains 1-3 during the development of Xenopus laevis in order to clarify the function of these three proteases. For the first time, we detected the localization of the three proteases at the protein level between one-cell stage and adult age. Their expression was weak at early stages, then increased at tadpole stage and decreased through metamorphosis and adult life. The calpain's expression was maximal during the period characterized by the appearance of organs and modelling process. These observations suggest that calpains play a crucial role during development. © 2010 Elsevier Ltd.
Violet F.,Catholic University of Lyon
Nanotechnology Law and Business | Year: 2010
Technical standardization and nanotechnology are two fields of knowledge liable to produce very fruitful results when combined, mainly because both spheres can be applied to every aspect of life, especially those involving technology. After all, isn't the whole word made up of atoms? And isn't this same world constantly being subjected to standards for the requirements of life in a society? This observation leads to overlaps that can be analyzed from an open-ended point of view: Nanotechnology is a new science. Its gradual arrival generates a strong need to create a structure, which could conceivably be brought about through technical standards. These standards can be prescriptive in some cases, and descriptive in others. In the context, they are fundamentally necessary. The work undertaken by both national and international standardization bodies testifies to the essential need. Like any new science, nanotechnology is initially intimidating to consumers, workers, and, more generally, most economic actors. Moreover, because the world is so special, adaptations to certain regulations must be envisaged. Here again, technical standards should be able to make a useful contribution by acting as information relays. French and other standardization working groups are operating with that aim in mind. All in all, a wealth of lessons can be learnt by analyzing the overlapping areas between technical standards and nanotechnology.
Danet F.,Catholic University of Lyon
Information Psychiatrique | Year: 2013
As psychiatrists look at man as a global subject and not as a series of organs, psychiatry occupies a marginal and therefore a fragile position in medicine. This position could prompt the profession and psychiatrists to use two strategies to avoid this: a connection with neurosciences to establish psychiatry as an "exact" science and the use of educational and normative rhetoric to establish a social legitimacy whereas its medical approach would possibly fail. Three other strategies appear to us more suitable to the professional identity of psychiatry in an attempt to establish its position: the promotion of research combining the so-called exact sciences as well as the humanities and social sciences, the assertion of its integration as well as its marginality faced with specialized medicine and the enhancement of a more complex rather than normative speech about the unconscious issues as regards individual groups and societies.
Gueullette J.-M.,Catholic University of Lyon
Ethique et Sante | Year: 2013
The reflection organized by the CME of a hospital about the decision to stop treatment was an opportunity to assess how much the analysis of this medical decision can introduce the whole of the professionals to the recognition of fundamental ethics. The Leonetti law of April 22nd, 2005 defines and establishes a framework for stopping treatment. It states that you can stop care, or do not implement them, if they are unnecessary or disproportionate, meanwhile respecting the duty of caring. These elements begin the ethical reflection. The law helps us to understand the distinction between law and ethics; it allows and prohibits certain things, but does not specify the details of the decision. We also find there the distinction between act and subject: it is not such care that would be rejected, but the context in which they would be applied, a context estimated in situation. Eventually, the reading highlights the link between the search for the good of the person and of the common good: caring is an imperative that lies within the limits of the humanity for nursing people. © 2013 Elsevier Masson SAS.
D'Hombres E.,Catholic University of Lyon
Journal of the History of Biology | Year: 2012
The notion of the 'division of physiological labour' is today an outdated relic in the history of science. This contrasts with the fate of another notion, which was so frequently paired with the division of physiological labour, which is the concept of 'morphological differentiation.' This is one of the elementary modal concepts of ontogenesis. In this paper, we intend to target the problems and causes that gradually led biologists to combine these two notions during the 19th century, and to progressively dissociate them, retaining only the concept of differentiation by the early 20th century. We shall adhere to the following: 1. The primitive economic concept of the division of labour is not a descriptive notion denoting a type of organisation of labour, but an etiological one: the idea of a causal relationship between this type of organization and the improvement of the whole. 2. This concept rapidly interested naturalists such as Henri Milne-Edwards, who were keen to find a rational ground for hierarchizing living forms based on anatomical complexity. 3. The validation of this notion in the realms of biology was subject to at least two conditions which were far from being fully satisfied. This did not prevent, however, the initial success of the concept of the division of physiological labour during the second half of the 19th century. 4. Finally, the gradual disqualification, within the Darwinian theoretical context, of the conception of an intrinsic hierarchical rank of organisms, led to a lack of interest in the concept of the physiological division of labour, at least in its non-Darwinian and non-ecological variant (the link between the division of labour within an organism and organic perfection). © 2010 Springer Science+Business Media B.V.