Liu T.,University of California at Davis |
Chen L.,University of California at Davis |
Kim E.,Catholic University of Daejeon |
Tran D.,University of California at Davis |
And 3 more authors.
Life Sciences | Year: 2014
Aims Mitochondrial dysfunction is an important part of the decline in cardiac function in heart failure. We hypothesized for hypothesized that there would be specific abnormalities in mitochondrial function and proteome with the progression of ischemic heart failure (HF). Main methods We used a high left anterior descending artery (LAD) ligation in 3-4 month old male rats to generate HF. Rats were studied 9 weeks post-ligation. Key findings Electron microscopy of left ventricle samples showed mitochondrial changes including decreased size, increased number, abnormal distribution, and cristae loss. Mitochondria in ischemic HF exhibited decreased total ATP, impaired mitochondrial respiration, as well as reduced complex I activity. Analysis of LV mitochondrial proteins by mass spectrometry was performed, and 31 differentially expressed proteins (p < 0.05) of more than 500 total proteins were identified. Of these proteins, 15 were up-regulated and 16 were down-regulated in the failing heart. A set of complex I proteins was significantly decreased, consistent with the impairment of complex I activity. There were distinct changes in mitochondrial function and proteome in ischemic HF. Although there were similarities, the distinction between the reported proteomic changed with TAC pressure overload induced HF and ischemic HF in the current study suggested different pathological mechanisms. Significance Specific changes in mitochondrial protein expression, which correlate with changes in mitochondrial function, have been identified in ischemic HF for the first time. © 2014 Elsevier Inc.
Blasi C.,Centro Diabete |
Kim E.,Catholic University of Daejeon |
Knowlton A.A.,University of California at Davis
Autoimmune Diseases | Year: 2012
The diabetes-atherosclerosis relationship remains to be fully defined. Repeated prolonged hyperglycemia, increased ROS production and endothelial dysfunction are important factors. One theory is that increased blood levels of heat shock protein (HSP)60 are proinflammatory, through activation of innate immunity, and contribute to the progression of vascular disease. It was hypothesized that improvement of diabetes control in patients presenting with metabolic syndrome would lower HSP60, and anti-HSP60 antibody levels and decrease inflammatory markers. Paired sera of 17 Italian patients, before and after intensive treatment, were assayed for cytokines, HSP60 and anti-HSP60 antibodies. As expected, intensive treatment was associated with a decrease in HgbA1C (P 0.001) and BMI (P 0.001). After treatment, there was a significant decrease in IL-6 (P 0.05). HSP60 levels were before treatment - 6.9 + 1.9, after treatment - 7.1 + 2.0 ng/mL (P = ns). Overall HSP60 concentrations were lower than published reports. Anti-HSP60 antibody titers were high and did not decrease with treatment. In conclusion, improvement of diabetic control did not alter HSP60 concentrations or antiHSP60 antibody titers, but led to a reduction of IL-6 levels. © 2012 Claudio Blasi et al.
Lee D.-H.,University of Ulsan |
Lee J.-S.,Pusan National University |
Yi J.-S.,Catholic University of Daejeon |
Cho W.,University of Washington |
And 2 more authors.
Spine Journal | Year: 2013
Background context: The proximity (<5 mm) of the plate to the adjacent disc space is known to be a critical risk factor for adjacent-level ossification development (ALOD). As plates provide many advantages including higher fusion rates and improved alignment, their use will continue. Instead, it is necessary to modify the plating techniques to minimize this complication. Purpose: To determine if our newer plating technique decreases the incidence of ALOD after anterior cervical plating. Study design: Retrospective matched cohort analysis of preoperative and postoperative radiographic data. Patient sample: One hundred patients were classified into two groups; conventional (C) and new (N) plating techniques. The control group (Group C) was matched to the study group (Group N) in a 1:1 fashion using matching criteria of age (within 5 years), gender, number of fusion levels, and comorbidities, including diabetes and tobacco use. Outcome measures: The lateral plain X-rays of cervical spine taken at postoperative 6 months and 2 years were used for analysis. Methods: In Group N, the cranial and caudal screws were started at the anterior end plate corners and angled away from the end plates so as to use the shortest possible plate and maximize the distance to the adjacent end plates. Group C was the historical control using a longer plate with more orthogonal screw angulation. On postoperative 6-week lateral films, the distances from the tip of the plate to both cranial and caudal adjacent discs (plate-to-disc distances) were measured. Based on the postoperative 2-year radiographs, the incidence of ALOD was determined, and the severity of ossification was classified on a scale ranging from Grade 0 (no ossification) to Grade 3 (complete bridging). Results: Mean plate-to-disc distances in Group N were significantly longer at both cranial and caudal adjacent levels than those in Group C (p<.001). The incidence of ALOD was significantly lower in Group N than in Group C, both at the cranial adjacent disc spaces (42% vs. 72%) and caudal adjacent disc spaces (20% vs. 42%) (p<.05). Severe ossification (Grade 2 or greater) also developed less frequently in Group N at cranial and caudal levels (6% vs. 20%, respectively; p<.05). Conclusions: The new technique of using a shorter plate with longer angulated screws resulted in significantly reduced incidence and severity of ALOD. © 2013 Elsevier Inc. All rights reserved.
Park S.E.,Catholic University of Korea |
Lee J.-U.,Catholic University of Daejeon |
Ji J.-H.,Catholic University of Korea
Knee Surgery, Sports Traumatology, Arthroscopy | Year: 2011
Both intraneural lipoma and chondroid lipoma have been reported in the previous literature as a separate disease entity on rare occasions. But intraneural chondroid lipoma has not been ever reported till now. So we present such a patient with an intraneural chondroid lipoma localized to common peroneal nerve. © 2010 Springer-Verlag.
Lee D.-S.,Catholic University of Daejeon |
Oh D.-S.,Korea Institute of Oriental Medicine |
Son C.-G.,Daejeon University
European Journal of Integrative Medicine | Year: 2014
Introduction: Many patients with ulcerative colitis often try a complementary medicine approach as western medications do not necessarily cure UC but can only reduce symptoms. This case report presents a combined therapy of traditional Chinese medicine (TCM) and conventional drugs as an integrative treatment for refractory ulcerative colitis. Method: A 27-year-old woman with a refractory ulcerative colitis had been treated with combination of TCM therapies and conventional drugs. We evaluated the improvement of disease status using periodic endoscopic findings and Mayo score assessment. Results: The symptoms including abdominal pain, frequent diarrhea mixed with blood and mucous discharge, and weight loss, had persisted with only occasional remission with corticosteroids and sulfasalazine treatment. Combination of TCM-based treatment remarkably improved all symptoms under use of only half-dose of sulfasalazine. The inflammation and bleeding disappeared at colonoscopy examination, with a significant reduction in Mayo score from 10 to 3 in 3 months. Conclusion: This case report proposes a potential strategy for treating severe active ulcerative colitis. © 2014 Elsevier GmbH.
Lee J.-S.,Korea Advanced Institute of Science and Technology |
Choi J.,Catholic University of Daejeon |
Yoo J.H.,Korea Advanced Institute of Science and Technology |
Kim M.,Chungbuk National University |
And 3 more authors.
Human Brain Mapping | Year: 2014
Semantic priming is affected by the degree of association and how readily a word is imagined. In the association effect, activity in the perisylvian structures including the bilateral inferior frontal gyrus, the left middle temporal gyrus, and the supramarginal gyrus was correlated. However, little is known about the brain regions related to the effect of imagery word under the preconscious condition. Forty word pairs for high (HA)-, low (LA)-, and nonassociation (NA), nonword (NW) conditions were presented. Each 40 association word pairs (HA and LA) included 20 high (HI) and 20 low (LI) imagery prime stimuli, using a visually presented lexical decision task. A trial consisted of 30 ms prime, 30 ms mask, 500 ms probe, and 2-8 s stimulus onset asynchrony. Brain activation was measured using functional magnetic resonance imaging during word discrimination. Behavioral data indicated that the shortest response time (RT) was given for HA words, followed by LA and NA, and NW showed the longest RT (P<0.01). RT was faster in HI than LI within HA, but not LA conditions (P<0.01). Functional neuroimaging showed that differential brain regions for high imagery (HI) and low imagery (LI) words within low prime-target word association were observed in the left precuneus, left posterior cingulate gyrus, and right cuneal cortex. The present findings demonstrate that the effect of the degree of imagery on semantic priming occurs during the early stage of language processing, indicating an "automatic imagery priming effect." Our paradigm may be useful to explore semantic deficit related to imagery in various psychiatric disorders. © 2014 The Authors.
Seo K.-S.,Chungnam National University |
Park J.-H.,Chungnam National University |
Heo J.-Y.,Chungnam National University |
Jing K.,Chungnam National University |
And 11 more authors.
Oncogene | Year: 2015
Hypoxia-inducible factor-1α (HIF-1α) is a transcription factor that has a central role in the regulation of tumour metabolism under hypoxic conditions. HIF-1α stimulates glycolytic energy production and promotes tumour growth. Sirtuins are NAD(+)-dependent protein deacetylases that regulate cellular metabolism in response to stress; however, their involvement in the hypoxic response remains unclear. In this study, it is shown that SIRT2-mediated deacetylation of HIF-1α regulates its stability in tumour cells. SIRT2 overexpression destabilized HIF-1α under hypoxic conditions, whereas HIF-1α protein levels were high in SIRT2-deficient cells. SIRT2 directly interacted with HIF-1α and deacetylated Lys709 of HIF-1α. Deacetylation of HIF-1α by SIRT2 resulted in increased binding affinity for prolyl hydroxylase 2, a key regulator of HIF-1α stability, and increased HIF-1α hydroxylation and ubiquitination. Moreover, a pharmacological agent that increased the intracellular NAD(+)/NADH ratio led to the degradation of HIF-1α by increasing SIRT2-mediated deacetylation and subsequent hydroxylation. These findings suggest that SIRT2-mediated HIF-1α deacetylation is critical for the destablization of HIF-1α and the hypoxic response of tumour cells.
Ji J.-H.,Catholic University of Daejeon |
Shafi M.,Catholic University of Daejeon |
Song I.-S.,Sun Hospital |
Kim Y.-Y.,Catholic University of Daejeon |
And 2 more authors.
Arthroscopy - Journal of Arthroscopic and Related Surgery | Year: 2010
Purpose: The purpose of this retrospective study was to evaluate the early results of arthroscopic treatment in patients with comminuted, displaced greater tuberosity (GT) fractures using the arthroscopic double-row suture anchor fixation (ADSF) technique. Methods: Between August 2004 and December 2007, we used the ADSF technique in 16 cases of isolated comminuted, displaced GT fractures. The early clinical results were evaluated in these patients at a mean of 24 months (range, 16 to 51 months) after surgery. There were 11 male and 5 female patients with a mean age of 56.5 years (range, 27 to 82 years). These 16 cases had at least 5 mm of displacement of the fracture fragments in any plane. For measurement of clinical outcomes, we assessed range of motion and evaluated the visual analog scale score; the University of California, Los Angeles (UCLA) rating scale; and the shoulder index of the American Shoulder and Elbow Surgeons. Results: At final follow-up, the visual analog scale score improved from 9.4 (range, 8 to 10 points) to 1.2 (range, 0 to 4 points), the mean UCLA score improved to 31 points (range, 21 to 35 points) postoperatively, and the American Shoulder and Elbow Surgeons score improved to 88.1 points (range, 81.5 to 100 points). According to the UCLA score, there were 3 excellent results, 11 good results, and 2 poor results. Mean forward flexion was 148.7° (range, 120° to 170°), mean abduction was 145° (range, 120° to 170°), mean external rotation in the neutral position was 24° (range, 10° to 40°), and internal rotation improved to the first lumbar vertebral level (from L3 to T7) at last follow-up. Conclusions: The early results of the ADSF technique used for displaced, comminuted GT fractures are encouraging, and arthroscopists should attempt to expand the indications for arthroscopic treatment of these fractures. Level of Evidence: Level IV, therapeutic case series. © 2010 Arthroscopy Association of North America.
Park S.-E.,Catholic University of Daejeon |
Kim J.-Y.,Kyung Hee University |
Cho S.-W.,St. Mary's College |
Rhee S.-K.,St. Mary's College |
Kwon S.-Y.,St. Mary's College
Journal of Shoulder and Elbow Surgery | Year: 2013
Background: This study evaluates the long-term results, including complication and revision rates, of different types of total elbow arthroplasty (TEA) with an average follow up of 13 years. Methods: Since 1984, a total of 84 primary TEAs have been performed in 77 patients at our institution. The patient's average age was 54.2 years. We performed unlinked TEA in 35 cases (Pritchard ERS since 1984 [n = 18], Kudo type 3 since 1991 [n = 17]), and semi-linked TEA in 49 cases (Pritchard Mark II since 1997 [n = 14], Coonrad-Morrey since 2001 [n = 35]). We assessed the patients for compliance to daily living guidelines (not to exceed 2.25 kg for repetitive lifting and 4.5 kg for single episode lifting), and followed up with them for an average of 13 years after primary TEA. We analyzed their results with regard to complication and revision rates as the type of TEA. Results: The mean Mayo Elbow Performance Score (MEPS) improved from preoperative 34 points to postoperative 84 points. The active flexion-extension elbow motion increased from 25°-94° preoperative to 12°-130° postoperative. The overall complication rate was 44.0% (37/84 cases); the rate was statistically higher in the unlinked group (62.9%, 22/35 cases) than in the semi-linked group (30.6%, 15/49 cases). Theoverall revision rate was 27.4% (23/84 cases); the rate was higher in the unlinked group (34.3%, 12/35 cases) than in the semi-linked group (22.4%, 11/49 cases). Conclusion: Semi-linked TEA has better outcomes than unlinked TEA with respect to complication and revision rates; but continuous efforts to develop a new TEA design for longevity, improved cementing technique, and supporting activities of daily living are needed to reduce complication and revision rates in the future. © 2013 Journal of Shoulder and Elbow Surgery Board of Trustees.
Kim H.J.,Catholic University of Daejeon
Interventional neuroradiology : journal of peritherapeutic neuroradiology, surgical procedures and related neurosciences | Year: 2011
We report our experience with endovascular treatment of supra-aortic arteries and follow-up results in patients with Takayasu's arteritis (TA) presenting with neurological symptoms. Of the 20 patients with TA who underwent cerebral angiography for neurological manifestations between May 2002 and May 2009, 12 (11 females, one male; mean age, 39 years; range 31-56 years) underwent endovascular treatment and evaluated outcome for 21 lesions, including nine common carotid arteries, four vertebral arteries, four subclavian arteries, two internal carotid arteries, and one brachiocephalic artery. Eight patients underwent multiple endovascular procedures for different lesions in single or multiple stages. Mean angiographic and clinical follow-up durations were 34 months (range, 11-79 months) and 39 months (range 11-91 months), respectively. Technical success was achieved for 20 procedures in 11 patients. One procedure failed, with 50% residual stenosis after stenting due to dense calcification of vessel walls. There were no procedure-related complications. Restenosis occurred at two lesions in two patients were treated by re-stenting. Asymptomatic occlusion occurred at two lesions in one patient. Ten patients remained in 0-1 on the modified Rankin scale (mRs) during mean 39 months. One patient, however, had a score of 3 on mRs due to a traumatic contusion during follow-up. One patient died from cardiac failure 36 months after successful angioplasty.Our data suggest that endovascular treatment of symptomatic supra-aortic lesions of TA is effective and durable in selected patients with neurologic symptoms.