Comodoro Rivadavia, Argentina

Catholic University of Cuyo
Comodoro Rivadavia, Argentina

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Martin Gimenez V.M.,Catholic University of Cuyo | Martin Gimenez V.M.,National University of Cuyo | Kassuha D.E.,Catholic University of Cuyo | Kassuha D.E.,CONICET | And 2 more authors.
Therapeutic Advances in Cardiovascular Disease | Year: 2017

Cardiovascular diseases are a major cause of disability and they are currently responsible for a significant number of deaths in a large percentage of the world population. A large number of therapeutic options have been developed for the management of cardiovascular diseases. However, they are insufficient to stop or significantly reduce the progression of these diseases, and may produce unpleasant side effects. In this situation, the need arises to continue exploring new technologies and strategies in order to overcome the disadvantages and limitations of conventional therapeutic options. Thus, treatment of cardiovascular diseases has become one of the major focuses of scientific and technological development in recent times. More specifically, there have been important advances in the area of nanotechnology and the controlled release of drugs, destined to circumvent many limitations of conventional therapies for the treatment of diseases such as hyperlipidemia, hypertension, myocardial infarction, stroke and thrombosis. © The Author(s), 2017.

Rey-Funes M.,University of Buenos Aires | Dorfman V.B.,Maimónides University | Ibarra M.E.,University of Buenos Aires | Pena E.,University of Buenos Aires | And 8 more authors.
Investigative Ophthalmology and Visual Science | Year: 2013

PURPOSE. To develop a time course study of vascularization and glial response to perinatal asphyxia in hypoxic-ischemic animals, and to evaluate hypothermia as possible protective treatment. METHODS. We used retinas of 7-, 15-, 21-, and 30-day-old male Sprague-Dawley rats that were exposed to perinatal asphyxia at either 378°C (PA) or 15°C (HYP). Born to term animals were used as controls (CTL). We evaluated the thickness of the most inner layers of the retina (IR), including internal limiting membrane, the retinal nerve fiber layer, and the ganglion cell layer; and studied glial development, neovascularization, adrenomedullin (AM), and VEGF by immunohistochemistry, immunofluorescence, and Western blot. RESULTS. A significant increment in IR thickness was observed in the PA group from postnatal day (PND) 15 on. This alteration was concordant with an increased number of new vessels and increased GFAP expression. The immunolocalization of GFAP in the internal limiting membrane and perivascular glia of the IR and in the inner processes of Müller cells was coexpressed with AM, which was also significantly increased from PND7 in PA animals. In addition, VEGF expression was immunolocalized in cells of the ganglion cell layer of the IR and this expression significantly increased in the PA group from PND15 on. The retinas of the HYP group did not show differences when compared with CTL at any age. CONCLUSIONS. This work demonstrates that aberrant angiogenesis and exacerbated gliosis seem to be responsible for the increased thickness of the inner retina as a consequence of perinatal asphyxia, and that hypothermia is able to prevent these alterations. © 2013 The Association for Research in Vision and Ophthalmology, Inc.

Romero-Cano R.,Jaume I University | Kassuha D.,Catholic University of Cuyo | Peris-Vicente J.,Jaume I University | Roca-Genoves P.,Jaume I University | And 2 more authors.
Analyst | Year: 2015

A micellar liquid chromatographic method has been developed for the simultaneous quantification of the pesticides thiabendazole and chlorpyrifos, as well as an alkylphenol, which is included in pesticide formulations, i.e., 4-tert-octylphenol, in water. A sample was filtered and directly injected, avoiding large extraction steps using toxic solvents, thus expediting the experimental procedure. The contaminants were eluted without interferences in <17 min, using a mobile phase of 0.15 M sodium dodecyl sulfate-6% 1-pentanol buffered at pH 3, running through a C18 column at 1 mL min-1 under the isocratic mode. This optimal mobile phase was selected using a statistical approach, which considers the retention factor, efficiency and peak shape of the analytes measured in only a few mobile phases. The detection was carried out by measuring absorbance at 220 nm. The method was successfully validated in terms of specificity, calibration range (0.5-10 mg L-1), linearity (r2 > 0.994), limit of detection and quantification (0.2-0.3; and 0.5-0.8 mg L-1, respectively), intra- and interday accuracy (95.2-102.9%), precision (<8.3%), and ruggedness (<9.3%). The stability in storage conditions (at least 14 days) was studied. The method was safe, inexpensive, produced little pollutant and has a short analysis time, thus it is useful for the routine analysis of samples. Finally, the method was applied to analyse wastewater from the fruit-processing industry, wastewater treatment plants, and in sewage water belonging to the Castelló area (Spain). The results were similar to those obtained by an already reliable method. This journal is © The Royal Society of Chemistry.

Bruno M.A.,McGill University | Bruno M.A.,Catholic University of Cuyo | Cuello A.C.,McGill University
Neurobiology of Aging | Year: 2012

Basal forebrain cholinergic neurons (BFCN), a system involved in learning and memory processes, are highly dependent on a continuous supply of biologically active nerve growth factor (NGF). Age-related cholinergic atrophy and cell loss in normal brains is apparently not complemented by reductions in the levels of NGF as could be expected. In the present work, cortical proNGF/NGF were immunoprecipitated from cortical brain homogenates from young and aged and behaviorally characterized rats and resolved with antinitrotyrosine antibodies to reveal nitration of tyrosine residues in proteins. Cortical proNGF in aged and cognitively impaired rats was found to be a target for peroxynitrite-mediated oxidative damage with correlative impact on decrease in choline acetyltransferase activity. These studies provide evidence for oxidative stress damage of NGF molecules in the cerebral cortex of cognitively impaired aged rats as previously shown in AD human brains. © 2012 Elsevier Inc.

Kruse M.S.,CONICET | Vega M.C.,CONICET | Rey M.,CONICET | Coirini H.,CONICET | And 2 more authors.
Journal of Endocrinology | Year: 2014

Gestational diabetes (GD) alters normal fetal development and is related to a diabetogenic effect in the progeny. Liver X receptors (LXRs) are considered to be potential drug targets for the regulation, treatment, or prevention of diabetes. The aim of this study was to evaluate early and late changes of LXR in the hippocampus and hypothalamus of the male and female offspring of control (CO) and diabetic (DO) mothers. We used an experimental model of streptozotocin-induced GD to assess the protein expression of LXRα (NR1H3) and LXRβ (NR1H2) by western blotting. The tissues were obtained from CO and DO animals at postnatal day 1 (1D), day 10 (10D), and day 35 (35D) and 9 months (9M). In CO, the LXR expression showed significant differences among the groups, which were tissue- and receptor-specific (P<0.05). Sex differences in CO were found only in the hypothalamus for LXRβ expression at 35D and 9M (P<0.05). When CO and DO were compared, differences between them were observed in the majority of the studied groups at 1D (male hippocampus, LXRα 31%and LXRβ 161%; female hippocampus, LXRβ 165%; male hypothalamus, LXRβ 182%; and female hypothalamus, LXRα 85%; P<0.05). However, these differences disappeared later with the exception of LXRβ expression in the male hypothalamus (P<0.05). The area under the curve during the glucose tolerance test correlated negatively with LXRβ in CO but not in DO animals. Moreover, in a male DO subpopulation this correlation was positive as it occurs in intolerant animals. These results indicate that GD affects hypothalamic LXR expression differently inmale and female offspring. © 2014 Society for Endocrinology.

Bodanza G.A.,CONICET | Alessio C.A.,Catholic University of Cuyo
Lecture Notes in Computer Science (including subseries Lecture Notes in Artificial Intelligence and Lecture Notes in Bioinformatics) | Year: 2014

An argument is reinstated when all its defeaters are in turn ultimately defeated. This is a kind of principle governing most argument systems in AI. Nevertheless, some criticisms to this principle have been offered in the literature. Assuming that reinstatement is prima facie acceptable, we analyze some counterexamples in order to identify common causes. As a result, we found that the problem arises when arguments in a chain of attacks are related by specificity. We argue that the reason is that non-maximally specific arguments can be reinstated originating fallacious justifications. Following old intuitions by Carl Hempel about inductive explanations, we propose a requirement of maximal specificity on defeasible arguments and introduce "undermining defeaters" which, in essence, facilitate the rejection of those arguments which do not satisfy the requirement. This ideas are formally defined using the DeLP system for defeasible logic programming. © 2014 Springer-Verlag.

PubMed | National University of Cuyo and Catholic University of Cuyo
Type: | Journal: Hipertension y riesgo vascular | Year: 2016

Hypertension is a medical condition considered one of the most important public health problems in developed countries, affecting around one billion people. Therefore, the study of its mechanisms, development and treatment is a priority. Of particular interest are the multiple contributing factors, and efforts by experts to fully understand it are also important. However, studies are currently insufficient and consequently, attention is focused on the exploration of new therapeutic approaches. This raises a growing interest in nanotechnology given the ability of certain structures to mimic the behavior of extracellular matrices. This opens a promising field in the treatment of diseases such as hypertension, where it stands to tissue engineering and its potential applications incorporating concepts such as controlled release drug, reduced side effects and receptor activation locally.

Bazan V.,Catholic University of Cuyo | Bazan V.,University of Buenos Aires
Revista de Derecho Politico | Year: 2014

Basically, this paper attempts: (i) to present the current situation of argentine federalism; (ii) to examine whether in reality have been fulfilled the guidelines established by the constitutional reform of 1994 to «strengthen» it; (iii) to analyze some issues of interest arising from federal dynamics, for example: (a) whether provincial constitutions can establish more rights than those contained in the Federal Constitution or provide rights already enshrined in this (and replicated in those) a stronger protective flow; (b) about the «federal clause» included in certain international human rights instruments, such as the Inter-American Convention on Human Rights, and (c) in relation to the impact of the conventionality control in federal relations; and, finally, (iv) to try to capturing the perspectives of federalism contained in the argentinian institutional horizon. © 2014 UNED.

Garcia J.J.,Catholic University of Cuyo
Cuadernos de bioética : revista oficial de la Asociación Española de Bioética y Ética Médica | Year: 2013

Bioethics is a rapidly growing discipline. It needs clear philosophical and theological foundations to remain a sure guide in promoting and defending the gift of life. In this paper it is not our task to perform a detailed analysis about the rich tenets of personalist bioethics. Instead, we make a comparative table between two bioethical models or proposals, Principialism and Personalism, similar in some respects, but different in others. So, the criteria that will guide us in deciding on biomedical practice will become clearer.

PubMed | Catholic University of Cuyo
Type: Journal Article | Journal: Cuadernos de bioetica : revista oficial de la Asociacion Espanola de Bioetica y Etica Medica | Year: 2013

Bioethics is a rapidly growing discipline. It needs clear philosophical and theological foundations to remain a sure guide in promoting and defending the gift of life. In this paper it is not our task to perform a detailed analysis about the rich tenets of personalist bioethics. Instead, we make a comparative table between two bioethical models or proposals, Principialism and Personalism, similar in some respects, but different in others. So, the criteria that will guide us in deciding on biomedical practice will become clearer.

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