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Arroyo Cabral, Argentina

Alegre E.A.,Catedra Salud Publica | De Biasio M.B.,Northeast National University | Ramirez N.N.,Catedra Salud Publica | Ruiz R.M.,Catedra Salud Publica | Bastiani C.E.,Catedra Salud Publica
Revista Veterinaria | Year: 2013

There are different diagnostic techniques to identify leptospiras; the polymerase chain reaction (PCR) is a method with high sensitivity and specificity. This study aimed to optimize DNA extraction from bacterial cultures and set conditions of multiplex PCR to differentiate saprophytes from pathogens leptospiras. For this, two techniques were tested: cetyl trimethyl ammonium bromide (CTAB) and boiling. Two sequences were analyzed, corresponding to a fraction of a conserved gene in both groups of bacteria (16S rRNA) and another in a fraction of a gene present only in the pathogenic leptospiras (LipL32). Two amplicons of 474 and 430 pb respectively were obtained using cultures of Leptospira icteroahemorragiae, L. canicola and apathogenic (Patoc I) as positive controls and water as negative control. It was concluded that there was no difference in the durability of DNA extracted from reference strains compare with CTAB digestion and boiling and its variants. Considering its lower cost and time saving, the latter represents a good alternative for PCR amplification.

Ruiz R.M.,Catedra Salud Publica | Ramirez N.N.,Catedra Salud Publica | Alegre A.E.,Catedra Salud Publica | Bastiani C.E.,Catedra Salud Publica | De Biasio M.B.,Servicio Veterinario de Biologia Molecular
Revista Veterinaria | Year: 2015

Leishmaniasis is a parasitic disease of great impact on public health. Feline leishmaniasis is rare, however, there is an increment in the number of cases reported around the world. In Argentina little information is available regarding the prevalence of this disease as well as the role of cats in its transmission. The objective of this work was to report a case of feline leishmaniasis in Corrientes City, Argentina, with a description of the clinical aspects and the identification of the parasite by means of molecular biology techniques. It can be concluded that this is a case of ulcerative cutaneous leishmaniasis, being the etiologic agent Leishmania (V) braziliensis.

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