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Slanac A.L.,Catedra de Fisiologia | Sgroppo S.C.,Catedra de Bromatologia | Kucseva C.D.,Instituto Nacional de Tecnologia Agropecuaria | Balbuena O.,Catedra de Nutricion y Alimentacion
Revista Veterinaria | Year: 2014

With the objective of characterize the protein fractions of Sorghastrum setosum ("sandysoil indiangrass") during its ruminal degradation, 3 g of acetone powder obtained from samples subjected to different ruminal incubation times were taken (0; 6; 12; 24; 48; 72 and 120 hours). Proteins present in the acetone residue were solubilized in Tris buffer pH 8 and quantitated by the Lowry method. The characterization was carried out by denaturing polyacrylamide gel electrophoresis (12% containing SDS) according to the modified Laemmli buffer system (1970). The qualitative analysis performed at zero hour showed intense bands of 87; 64; 58; 55; 51; 47; 46; 44; 42; 27 and 14 kDa. For the protein profiles, intense bands of 46; 42 and 14 kDa were detected, as well as others of lower intensity of 64; 58 and 51 and finally a group of high molecular weight in the region of 87 kDa. During the degradation process, modifications of the protein profile were observed, being detected new bands of high and half PM, as well as the appearance and increase of low PM bands. During the process of ruminal degradation it was observed that the bands of 24, 14 and 55 kDa were present during the 120 hours of study; this prolonged permanency indicates the scarce ruminal degradability of this gramineous. Source


Teijeiro J.M.,CONICET | Dapino D.G.,Catedra de Fisiologia | Marini P.E.,CONICET
Biological Research | Year: 2011

In their journey through the oviduct some subpopulations of sperm are preserved in a reservoir, while others are negatively selected. Sperm binding glycoprotein (SBG) is a pig oviductal epithelial cell glycoprotein that produces, under capacitating conditions, acrosome alteration, p97 tyrosine-phosphorylation and reduction of the motility of sperm. In this paper, we show that SBG is accessible at the extracellular surface of the oviductal epithelial cells, supporting a sperm interaction biological role in situ. We analyze the possible dependence of the tyrosine-phosphorylation of p97 on the PKA mechanism, finding that apparently it is not PKA dependent. Also, after SBG treatment the phosphorylated proteins locate mainly at the detached periacrosomal region and at the tail of sperm; the latter may be related to SBG's motility reduction effect. The study of the time course effect of SBG on sperm as detected by chlortetracycline (CTC) staining and of its binding to sperm by immunodetection in conjunction with CTC, shows results in agreement with the hypothesis that this glycoprotein is involved in the alteration of acrosomes in a specific sperm subpopulation. The results suggest that SBG may be part of a mechanism for negative selection of sperm. Source


Slanac A.L.,Catedra de Fisiologia | Kucseva C.D.,Instituto Nacional de Tecnologia Agropecuaria | Balbuena O.,Instituto Nacional de Tecnologia Agropecuaria | Rochinotti D.,Instituto Nacional de Tecnologia Agropecuaria
Revista Veterinaria | Year: 2013

To evaluate parameters of ruminal degradation of crude protein (CP) in Paspalum notatum, samples of 15, 30 and 45 days of re-growth were cut at 5 cm height at each season of the year. Rumen-cannulated steers of 550 kg body weight were used to run in situ procedure. Incubation times were: 0, 3, 6, 12, 24, 48, 72 and 120 h. Samples were analyzed for CP and data were fitted to Orskov y McDonald model. Resulting degradation parameters as well as effective CP degradation were analyzed by the GLM procedure of SAS package. Only the soluble fraction differed among seasons. Potentially degradable fraction, degradation rate and effective ruminal degradation were different only at 45 days of re-growth. Other CP ruminal degradation parameters were not affected by days of re-growth or season of the year, suggesting that the method may not be adequate for detecting effect of re-growth and season. Source


The aim of this work consisted to study the clinical and hematological effects that included blood coagulation and antibody production of a group of three horses immunized for the production of monovalent serum against Bothrops alternatus venom, in order to correlate the results with the humoral immune response of the animals. The results showed that immunization of horses with the essayed protocol induce slight clinic alterations and discrete changes in some parameters of the hemogram. Results also showed variations in the neutralizing capacity of the serum on different venom components, particularly toxins that affect blood coagulation. Also, it was verified a direct relationship between the rate of white blood cells and serum immunoglobulin production. Furthermore, they also indicate that the three-month rest period may be considered as too long as it causes a marked decrease in antibody titles, losing the serum its neutralizing capacity to venom compounds. Nevertheless, after re-administration of venom horses provided a rapid reposition of anti-toxins against B. alternatus venom, tolerating high venom doses during this second period of immunization. Source


Gonzalez Vegas J.A.,Catedra de Fisiologia
Acta Cientifica Venezolana | Year: 2012

Sensory experiences determining different perceptions of the same reality in different subjects, or in the same subject, are presented. The effect of culture in our perception of reality and the absence of an absolute truth are discussed. Our perception of reality is rather the manifestation of a social consensus about representations of this reality. Source

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