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Suipacha, Argentina

Petenatti M.E.,National University of San Luis | Gette M.A.,Catedra de Farmacobotanica | Cami G.E.,Laboratorio Of Quimica Inorganica | Popovich M.C.,Catedra de Farmacobotanica | And 3 more authors.
Revista de la Facultad de Ciencias Agrarias | Year: 2014

Melissa officinalis L., so called "Melissa" or "Toronjil", is a perennial aromatic herb, whose leaves are used in traditional medicine as a carminative, digestive and sedative, both in simple as in mixtures. Meanwhile, Nepeta cataria L., commonly called "Cat mint" or "Toronjil", with some similar properties, often replacing M. officinalis in the market, although their chemical composition is not completely matched, and contains an iridoid potentially toxic (nepetalactone). It is therefore necessary to establish diacritic parameters to differentiate these species, both at crude drug level, mixtures and extracts. Samples from various sources in Argentina were studied and documental specimens are preserved in the Herbarium UNSL. Anatomical sections were analyzed, and quantitative micrographic parameters were obtained, together with HPLC and FTIR spectra from methanolic and aqueous lyophilized extracts. Significant differences were detected in the prevailing smell foliage, morphology of leaves and inflorescences, trichomata type, palisade ratio, veinlet termination number, rosmarinic acid concentration (with distinctive HPLC profiles), and the CO/CH relationships obtained by FTIR from the extracts, that together allow adequate differentiation of both drugs, even when they were ground or powdered. © 2014, Universidad Nacional de Cuyo. All rights reserved.

The Simaroubaceae (sensu lato) family is represented in Argentina by six genera and eight species, seven of which are native and only one non-autochthonous; some are used in folk medicine as tonic, insecticides and pesticides. Leaves were cut previous paraffin embedding and diaphanization. Longitudinal and cross-sectional cuts were made on cortex and leños and were cut and macerated. They were stained with Safranine-Fast-green and Cresyl Violet. Microscopic examination was performed by light microscopy and SEM. In the present work leaves, cortex and leños from Alvaradoa subovata, Picramnia parvifolia, Picramnia sellowii and Castela coccinea were morpho-anatomically analyzed in order to determine diagnosis characters to identify diagnostic characters to ensure the identity and quality of these resources. In leaves, namely, 1. presence or absence of gland hairs, mesophile type, presence of mucilage; 2. cortex: periderm, radii types, crystals, sclerenchyma elements; 3. leño size, pores location, radii, and axial parenchyma, among others. Complete the presentation with photomicrographs and keys in order to provide adequate differentiation between entities. © 2010 The Authors.

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