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Parma R.,El Hospital Provincial Del Centenario | Belbuzzi C.,Medica Responsable de la Coordinacion en Zona sur de la Provincial | Kirilovsky D.,Medica Responsable de la Coordinacion en Zona Norte de la Provincial | Massoni C.,Bioquimica Especialidad Endocrinologia de la Ciudad de Rosario | And 3 more authors.
Revista Argentina de Endocrinologia y Metabolismo | Year: 2013

A total of 1,470 primary school students were evaluated to monitor the prevalence of endemic goiter in four cities of Santa Fe province: Reconquista (404) and Villa Ocampo (294) situated in the north, and Rufino (317) and Venado Tuerto (455) situated in the south of the province. Their age ranged from 6 to 14 years. Neck palpation was performed in all students; for the sake of consistency with monitoring performed in other provinces, palpation was performed by only one physician (HN). The goiter grading system was the same used in other surveys. After palpation, urine samples for iodine determination were randomly collected (109 from Reconquista, 105 from Villa Ocampo, 113 from Rufino and 139 from Venado Tuerto). Iodine was also measured in salt samples collected from the children's homes (337 from Reconquista, 227 from Villa Ocampo, 295 from Rufino and 422 from Venado Tuerto). Prevalence of goiter was 2.6 % without differences among cities. Urine iodine levels were significantly different between northern and southern cities: Reconquista had a mean of 238.6 ± 116 ?g/L (median 213 ?g/L) and Villa Ocampo had a mean of 241.6 ± 223.5 ?g/L (median 188 ?g/L), while in Rufino the mean was 544 ± 349 ?g/L (median 442 ?g/L) and in Venado Tuerto it was 550 ± 375 ?g/L (median 418 ?g/L; mean ± SD). Iodine contents in salt were similar across the province, with a mean of 35.5 mg/kg. Prevalence of salt samples with iodine concentration 15 mg/kg was 0.3 % in Reconquista, 1.3 % in Villa Ocampo, 3 % in Rufino, and 4 % in Venado Tuerto. We conclude that the province of Santa Fe, Argentina, is free from endemic goiter. © 2013 por la Sociedad Argentina de Endocrinologia y Metabolismo.

Slanac A.L.,Catedra de Fisiologia | Sgroppo S.C.,Catedra de Bromatologia | Kucseva C.D.,Instituto Nacional de Tecnologia Agropecuaria | Balbuena O.,Catedra de Nutricion y Alimentacion
Revista Veterinaria | Year: 2014

With the objective of characterize the protein fractions of Sorghastrum setosum ("sandysoil indiangrass") during its ruminal degradation, 3 g of acetone powder obtained from samples subjected to different ruminal incubation times were taken (0; 6; 12; 24; 48; 72 and 120 hours). Proteins present in the acetone residue were solubilized in Tris buffer pH 8 and quantitated by the Lowry method. The characterization was carried out by denaturing polyacrylamide gel electrophoresis (12% containing SDS) according to the modified Laemmli buffer system (1970). The qualitative analysis performed at zero hour showed intense bands of 87; 64; 58; 55; 51; 47; 46; 44; 42; 27 and 14 kDa. For the protein profiles, intense bands of 46; 42 and 14 kDa were detected, as well as others of lower intensity of 64; 58 and 51 and finally a group of high molecular weight in the region of 87 kDa. During the degradation process, modifications of the protein profile were observed, being detected new bands of high and half PM, as well as the appearance and increase of low PM bands. During the process of ruminal degradation it was observed that the bands of 24, 14 and 55 kDa were present during the 120 hours of study; this prolonged permanency indicates the scarce ruminal degradability of this gramineous.

Binaghi M.J.,Catedra de Bromatologia | Binaghi M.J.,University of Buenos Aires | Cagnasso C.E.,Catedra de Bromatologia | Cagnasso C.E.,University of Buenos Aires | And 10 more authors.
Archivos Latinoamericanos de Nutricion | Year: 2011

Home-made diets are the most frequently used complementary foods. In the present work we evaluated iron and zinc availability in a usually consumed infant diet containing either iron-fortified bread with different iron sources: ferrous sulfate, ferrous bisglycinate, NaFeEDTA. We also used non-fortified bread with absorption promoters: ascorbic acid, sodium citrate, Na2EDTA, combined with different beverages. The diet (potato, pumpkin, grits, bread, and apple) was combined with water, milk, tea, a soft drink and an orange-based artificial drink. Mineral dialyzability (D) as an indicator of potential availability was determined using an in vitro method. Statistical analysis was performed by ANOVA, and a posteriori Tukey test. There were no significant differences in FeD between diets with ferrous sulfate or ferrous bisglycinate fortified bread; in NaFeEDTA fortified bread it increased significantly (p<0.05). Iron D increase was greater in diets with bread containing absorption promoters than in those with fortified bread. The orange-based artificial drink increased FeD, while tea and milk decreased it significantly (p<0.05). Zinc D increased significantly when the bread was fortified either with ferrous sulfate or NaFeEDTA, but remained unchanged in diets with ferrous bisglycinate fortified bread. The addition of tea or milk decreased ZnD while the orange-based artificial drink increased it significantly (p<0.05). Regarding absorption promoters, the greater values both in FeD and ZnD were observed in diets with iron nonfortified bread containing Na2EDTA.

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