University Camilo Castelo Branco
Sao Paulo, Brazil
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Pires-Oliveira D.A.A.,University Camilo Castelo Branco | Oliveira R.F.,University Camilo Castelo Branco | Pacheco-Soares C.,Institute Pesquisa e Desenvolvimento | Rocha R.F.,São Paulo State University
Osteoporosis International | Year: 2010

The aim of the present study was to determine the action of AsGA laser irradiation on bone repair in the tibia of osteopenic rats. The animals were randomly divided into eight experimental groups according to the presence of ovarian hormone (sham group) or the absence of the hormone (OVX group), as well as being irradiated or non-irradiated. Low-level 904-nm laser (50 mJ/cm 2) accelerated the repair process of osteopenic fractures, especially in the initial phase of bone regeneration. Introduction: The development of new techniques to speed the process of bone repair has provided significant advances in the treatment of fractures. Some attention recently focused on the effects of biostimulation on bone. Methods: Forty-eight adult rats were randomly divided into eight experimental groups (six animals in each group) according to the presence of ovarian hormone (sham group) or absence of the hormone (ovariectomized (OVX) group) as well as being irradiated or non-irradiated. For the application of low-level laser therapy, the animals were anesthetized with one third of the dose sufficient to immobilize the animal and irradiated with AsGa laser (904 nm, 50 mJ/cm2 for 2 s, point form and in contact). The control animals received the same type of manipulation as the irradiated animals, but with the laser turned off. Half of the animals were killed 7 days following the confection of the bone defect, and the other half were killed 21 days after the surgery. After complete demineralization, the tibias were cut cross-sectionally in the central region of the bone defect and embedded in paraffin blocks. The blocks were then cut in semi-seriated slices and stained with hematoxylin and eosin. Results: There was new bone formation in the animals in the OVX group with laser treatment killed after 7 days (p<0.001). The lowest percentage of bone formation was observed in the OVX without laser killed after 7 days (p>0.05). All animals killed after 21 days exhibited linear closure of the lesion. Conclusion: Low-level 904-nm laser (50 mJ/cm2) accelerated the repair process of osteopenic fractures, especially in the initial phase of bone regeneration. © 2010 International Osteoporosis Foundation and National Osteoporosis Foundation.

Cassenote A.J.F.,University of Sao Paulo | Pinto Neto J.M.,University Camilo Castelo Branco | Rubinsky-Elefant G.,University of Sao Paulo
PLoS Neglected Tropical Diseases | Year: 2014

Background:Toxocariasis is a worldwide helminthic zoonosis caused by infection with the larvae of the ascarid worms that comprise the Toxocara spp. Children are particularly prone to infection because they are exposed to the eggs in sandboxes and playgrounds contaminated with dog and cat feces. Certain behaviors, such as a geophagy habit, poor personal hygiene, a lack of parental supervision, close contact with young dogs, and ingestion of raw meat, as well as gender, age, and socioeconomic status, affect the prevalence of the disease. However, previous studies of the risk factors for toxocariasis have generally produced inconsistent results. An epidemiological cross-sectional study was conducted to evaluate the seroprevalence of IgG anti-Toxocara spp. antibodies and associated factors in schoolchildren from a region in the southeast of Brazil.Methodology/Principal Findings:A total of 252 schoolchildren aged 1 to 12 years (120 males and 132 females) were assessed. An enzyme-linked immunosorbent assay based on Toxocara canis larval excretory-secretory antigens was used to determine outcomes. A questionnaire was used to collect information on children, family, and home characteristics. Clinical and laboratory data completed the dataset investigated in this study. Seroprevalence was 15.5% (95%CI 11.5-19.8). Geophagy (aPR 2.38 [95%CI 1.36-4.18], p-value 0.029) and the habit of hand washing before meals (aPR 0.04 [95%CI 0.01-0.11], p-value ≤0.001) were factors associated with increased and decreased seroprevalence, respectively. The income factor and its related variables lost statistical significance after adjustment with a multiple Poisson regression model.Conclusions/Significance:The current study confirms that toxocariasis is a public health problem in the evaluated area; modifiable factors such as soil contact and personal hygiene appear to have a greater influence on the acquisition of infection than sociodemographic attributes, thus representing direct targets for disease prevention and control. © 2014 Cassenote et al.

Cruz R.A.,University of Sao Paulo | Pilla V.,University Camilo Castelo Branco | Catunda T.,University of Sao Paulo
Journal of Applied Physics | Year: 2010

A recently developed thermal lens spectrometry configuration has been used to study CdSe/ZnS core-shell quantum dots (QDs) suspended in toluene and tetrahydrofuran (THF) solvents. The special features of this configuration make it very attractive to measure fluorescence quantum yield () excitation spectrum since it simplifies the measurement procedure and consequently improve the accuracy. Furthermore, the precision reached is much higher than in conventional photoluminescence (PL) technique. Two methods, called reference sample and multiwavelength have been applied to determine , varying excitation wavelength in the UV-visible region (between 335-543 nm). The and PL spectra are practically independent of the excitation wavelength. For CdSe/ZnS QDs suspended in toluene we have obtained =76±2%. In addition, the aging effect on and PL has been studied over a 200 h period for QDs suspended in THF. © 2010 American Institute of Physics.

Barbosa D.,University Camilo Castelo Branco | De Souza R.A.,Instituto Federal Of Educacao | Xavier M.,Federal University of Minas Gerais | Da Silva F.F.,Instituto Federal Of Educacao | And 2 more authors.
Lasers in Medical Science | Year: 2013

The aim of this study was to evaluate the process of bone repair in rats submitted to low-level laser therapy using optical densitometry. A total of 45 rats which underwent femoral osteotomy were randomly distributed into three groups: control (group I) and laser-treated groups using wavelengths in the red (λ, 660-690 nm) and in the infrared (λ, 790-830 nm) spectra (group II and group III, respectively). The animals (five per group) were killed after 7, 14, and 21 days and the femurs were removed for optical densitometry analysis. Optical density showed a significant increase in the degree of mineralization (gray level) in both groups treated with the laser after 7 days. After 14 days, only the group treated with laser therapy in the infrared spectrum showed higher bone density. No differences were observed between groups after 21 days. Such results suggest the positive effect of low-level laser therapy in bone repair is time- and wavelength-dependent. In addition, our results have confirmed that optical densitometry technique can measure bone mineralization status. © 2012 Springer-Verlag London Ltd.

Neto O.P.,Texas A&M University | Neto O.P.,University Camilo Castelo Branco | Christou E.A.,Texas A&M University
Journal of Neurophysiology | Year: 2010

Rectification of EMG signals is a common processing step used when performing electroencephalographic-electromyographic (EEG-EMG) coherence and EMG-EMG coherence. It is well known, however, that EMG rectification alters the power spectrum of the recorded EMG signal (interference EMG). The purpose of this study was to determine whether rectification of the EMG signal influences the capability of capturing the oscillatory input to a single EMG signal and the common oscillations between two EMG signals. Several EMG signals were reconstructed from experimentally recorded EMG signals from the surface of the first dorsal interosseus muscle and were manipulated to have an oscillatory input or common input (for pairs of reconstructed EMG signals) at various frequency bands (in Hz: 0-12, 12-30, 30-50, 50-100, 100-150, 150-200, 200-250, 250-300, and 300-400), one at a time. The absolute integral and normalized integral of power, peak power, and peak coherence (for pairs of EMG signals) were quantified from each frequency band. The power spectrum of the interference EMG accurately detected the changes to the oscillatory input to the reconstructed EMG signal, whereas the power spectrum of the rectified EMG did not. Similarly, the EMG-EMG coherence between two interference EMG signals accurately detected the common input to the pairs of reconstructed EMG signals, whereas the EMG-EMG coherence between two rectified EMG signals did not. The frequency band from 12 to 30 Hz in the power spectrum of the rectified EMG and the EMG-EMG coherence between two rectified signals was influenced by the input from 100 to 150 Hz but not from the input from 12 to 30 Hz. The study concludes that the power spectrum of the EMG and EMG-EMG coherence should be performed on interference EMG signals and not on rectified EMG signals because rectification impairs the identification of the oscillatory input to a single EMG signal and the common oscillatory input between two EMG signals. © 2010 The American Physiological Society.

Neto O.P.,Texas A&M University | Neto O.P.,University Camilo Castelo Branco | Baweja H.S.,Texas A&M University | Christou E.A.,Texas A&M University
Muscle and Nerve | Year: 2010

The purpose of this study was to compare the capability of interference and rectified electromyography (EMG) to detect changes in the beta (13-30-HZ) and Piper (30-60-HZ) bands when voluntary force is increased. Twenty adults exerted a constant force abduction of the index finger at 15% and 50% of maximum. The common oscillations at various frequency bands (0-500 HZ) were estimated from the first dorsal interosseous muscle using cross wavelets of interference and rectified EMG. For the interference EMG signals, normalized power significantly (P < 0.01) increased with force in the beta (9.0 ± 0.9 vs. 15.5 ± 2.1%) and Piper (13.6 ± 0.9 vs. 21 ± 1.7%) bands. For rectified EMG signals, however, the beta and Piper bands remained unchanged (P > 0.4). Although rectified EMG is used in many clinical studies to identify changes in the oscillatory drive to the muscle, our findings suggest that only interference EMG can accurately capture the increase in oscillatory drive from 13 to 60 HZ with voluntary force. © 2010 Wiley Periodicals, Inc.

Kerppers I.I.,University Estadual do Centro Oeste Rua Vicente Machado | de Lima C.J.,University Camilo Castelo Branco | Fernandes A.B.,University Camilo Castelo Branco | Villaverde A.B.,University Camilo Castelo Branco
Lasers in Medical Science | Year: 2014

Collagen I is not only responsible for maintaining the integrity of most tissues due to its mechanical properties, but also for its active participation in the functionality of tissues because of its interaction with cells present in the extracellular matrix. The synthesis of collagen begins with tissue injury and remains until the end of the healing process. The use of non-coherent light for healing processes is still understudied. This procedure stands out as a biostimulation method for tissue repair, which increases local circulation, cell proliferation, and collagen synthesis. This study sought to quantify collagen I in the healing process after the treatment of wounds with the light-emitting diode (LED) treatment. The histologic analysis with tissue samples stained with picrosirius red showed a statistical difference between the positive controls, LED 627 and LED 945 nm groups; the group treated with LED 627 nm showed a predominance of mature collagen. The immunohistochemical analysis showed a statistically significant high concentration of collagen I in the LED 945 nm group. The irradiation of wounds with the higher wavelength (945 nm) used in the study produced the best activity of collagen I formation in experimental model. © 2014, Springer-Verlag London.

Franca R.F.,University Camilo Castelo Branco | De Oliveira H.P.M.,Federal University of Pelotas | Pedrosa V.A.,São Paulo State University | Codognoto L.,Federal University of São Paulo
Diamond and Related Materials | Year: 2012

In this study, a method for electroanalytical determination of carbendazim (CBZ) and fenamiphos (FNP) in natural and spiked water was developed using square wave voltammetry in Na 2HPO 4 0.1 mol L - 1 as supporting electrolyte. The calibration curve for carbendazim detection presented good linearity in the concentration range of 0.50 to 15.0 μM, with a sensitivity of 0.080 A/mol L - 1 and a linearity of 0.998. The oxidation of fenamiphos on BDD electrode shows a dynamic range of concentration of 0.5 to 25.0 μM and sensibility of 0.14 A/mol L - 1. The recovery experiments showed values between 70 and 100% for spiked samples thus indicating the feasibility of the electroanalytical methodology to quantify CBZ and FNP in pure or natural waters. © 2012 Elsevier B.V.

Pilla V.,Federal University of Uberlandia | Munin E.,University Camilo Castelo Branco
Journal of Nanoparticle Research | Year: 2012

The thermo-optical parameters of cadmium selenide/zinc sulfide (CdSe/ZnS) core-shell quantum dots (QDs) suspended in aqueous solutions were measured using a Thermal Lens (TL) technique. TL transient measurements were performed using the mode-mismatched dual-beam (excitation and probe) configuration. A He-Ne laser at kp = 632.8 nm was used as the probe beam, and an Ar? laser (at ke = 514.5 nm) was used as the excitation beam to study the effect of the core sizes (2-4 nm) of CdSe/ZnS nanocrystals functionalized with amine (R-NH2) or carboxyl (R-COOH) groups. The average values of the thermal diffusivity D = (1.48 ± 0.06) 9 10-3 cm2/s obtained for QDs samples are in good agreement with the pure water solvent result. The fraction thermal load (u) and radiative quantum efficiencies (g) of the functionalized CdSe/ZnS QDs were determined and compared with non-functionalized CdSe/ZnSQDs. The obtained g values for non-functionalized CdSe/ZnSare slightly higher than those for theQDs functionalized with amine or carboxyl groups. © Springer Science+Business Media B.V. 2012.

Baldin E.L.L.,São Paulo State University | Beneduzzi R.A.,University Camilo Castelo Branco
Journal of Pest Science | Year: 2010

The objective of this study was to investigate the potential resistance mechanisms to Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) B biotype, in eight Cucurbita pepo varieties. Attractivity, oviposition preference, and nymphal development were evaluated under greenhouse and laboratory conditions. Variety Bianca was the least attractive to adult whiteflies in multi-choice tests. The whiteflies showed oviposition nonpreference (antixenosis) for Novita Plus, Bianca and Sandy in multi-choice test, and similar results were observed in no-choice tests with varieties Bianca, Novita, Caserta Cac and Sandy. Sandy expressed high antibiosis levels against whitefly nymphs, leading to 63. 9% mortality. The high level of resistance in variety Sandy can serve as base for genetic improvement of C.pepo focusing on the development of resistant varieties to B. tabaci B biotype. © 2010 Springer-Verlag.

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