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Masoudifard M.,University of Tehran | Vajhi A.R.,University of Tehran | Moghim M.,Ecology Research Institute of the Caspian Sea | Nazari R.M.,Rajaei Sturgeon Reproduction and Stock Enhancement Center | And 2 more authors.
Journal of Applied Ichthyology | Year: 2011

The objective of this study was to test existing ultrasound technology for use in early sex identification in beluga sturgeon (3-years-old fish) while including some methodological adjustments. Eighty cultured Beluga sturgeons aged 3years underwent ultrasonographic examination using a high frequency transducer for sex determination. Sexing of the sturgeons was based on the shape and echogenicity (brightness) of the gonad and surrounding fat tissue in sonographic scans. Accuracy of ultrasound sex determinations were confirmed through directly inspecting the gonads by a small incision in the right or left ventral body wall. Females were identified with 96.7% accuracy (n=31) and males with 97.9% accuracy (n=49). Overall accuracy of sex determination using ultrasonography was 97.5%. Using high frequency transducer and transverse scanning just over the swim bladder in 3-year-old beluga may have been contributed in high accuracy sex detection. These technically improvements may be considered an advantage for beluga culture because this fish matures extremely late compared to other sturgeon species. © 2011 Blackwell Verlag, Berlin.

Pourgholam M.A.,Caspian Sea International Sturgeon Research Institute | Khara H.,Islamic Azad University at Lahijan | Safari R.,Ecology Research Institute of the Caspian Sea | Sadati M.A.Y.,Caspian Sea International Sturgeon Research Institute | Aramli M.S.,Urmia University
Probiotics and Antimicrobial Proteins | Year: 2016

We investigated the effects of Lactobacillus plantarum used as a dietary supplement on the growth performance and innate immune response in juvenile Siberian sturgeon Acipenser baerii. Juvenile fish (14.6 ± 2.3 g) were fed three experimental diets prepared by supplementing a basal diet with L. plantarum at different concentrations [1 × 107, 1 × 108 and 1 × 109 colony-forming units (cfu) g−1] and a control (non-supplemented basal) diet for 8 weeks. Growth performance indices were increased in fish fed the 1 × 108 cfu g−1L. plantarum diet compared to the other groups. There was an increased innate immune response in fish fed the experimental diets. The highest levels of lysozyme activity, total immunoglobulin (IgM) and complement component 3 (C3) were observed in fish fed the diet containing L. plantarum at a concentration of 1 × 108 cfu g−1, but there was no significant difference in the level of complement component 4 (C4) in fish fed the experimental diets or the control diet. The present study underlying some positive effects (growth performance and immune indices) of dietary administration of L. plantarum at a concentration of 1 × 108 cfu g−1 in the Siberian sturgeon. © 2015, Springer Science+Business Media New York.

Mohamadian S.,Islamic Azad University at Tehran | Rezavani Gilkolaei S.,Iranian Fisheries Research Organization IFRO | Rouhollahi S.,Iran University of Science and Technology | Taghavi M.J.,Ecology Research Institute of the Caspian Sea | And 3 more authors.
Iranian Journal of Fisheries Sciences | Year: 2012

Population genetic structure of Vimba vimba persa was investigated using microsatellite markers from 40 regions along the Iranian coastline of the Southern Caspian Sea (Anzali lagoon and Havigh River in Gilan province, BabolRoud River in Mazandaran province and GorganRoud River in Golestan province). Genomic DNA from 121 specimens was extracted from fin tissue by the Phenol-Chlorophorm method and PCR reaction was accomplished with 17 microsatellite primers, out of 17 microsatellite primers 13 loci were amplified, in which 10 of them were amplified with reasonable polymorphism and 3 were monomorphism. A total of 302 alleles were identified on average 7.55. Observed and expected heterozygosity averages were 0.80 and 0.77 respectively. Most cases significantly deviated from Hardy-Weinberg equilibrium (p≤0.01). The estimation of Fst (p≤0.01) revealed significant population structuring and an estimation of the four population of Vimba vimba persa was identified in the Caspian Sea in which restocking of these species should be considered.

Rezvani Gilkolaei S.,Iranian Fisheries Research Organization | Safari R.,Golestan University | Laloei F.,Ecology Research Institute of the Caspian Sea | Taqavi J.,Ecology Research Institute of the Caspian Sea | Matinfar A.,Iranian Fisheries Research Organization
Iranian Journal of Fisheries Sciences | Year: 2011

Sampling was done using 90 post larvae which were produced by reproduction of some broodstocks of Fenneropenaeus indicus in one day and reared in the same situation for 4 months. Samples were divided into 3 groups: high, medium and low growth (based on weight and length). Genomic DNA was extracted from muscle tissue using the phenol-chloroform method. The polymerase chain reaction (PCR) was carried out using 21 RAPD loci and PCR products were separated on 3% Agarose gel. From 21 loci studied, 12 produced polymorphic bands. The most polymorphic produced bands using OPAQ 9 and the least by OPAQ 7. Search for specific markers in F. indicus one specific band was observed in the low growth group using OPAQ4. The highest genetic distance (0.457) was between the low growth group and the medium and the lowest (0.091) between high growth and medium groups, therefore the highest genetic identity (0.912) was between high growth and medium groups and the lowest (0.633) between low growth group and the medium. Neighbor-joining resulted in two groups, the first including high and medium growth groups and the second low growth group. It appears that low growth group depended on separated population. Considering the mean weight of F1 (mean weight of 90 specimens) (16.25±1.5 g), parental generation mean weight of 15 ±1.2 and mean weight of parent 31.6 g, response to selection (R) and heritability for growth in this species were estimated to be 1.2±0.2 and 0.07±0.01 respectively.

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