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Patent
CAS Institute of Zoology | Date: 2016-11-14

A monoclonal antibody against human PrPc protein capable of reducing the expression level of transcription factor Twist1 in a targeting mode and inducing macrophage and NK cells to target to rectal cancer tumor cells. The combined drug therapy with the antibody and cetuximab also exhibits inhibitory effect on tumor better than administration of the antibody or cetuximab alone.


A cDNA, including the nucleotide sequence represented by SEQ ID NO: 2. A cDNA, including a nucleotide sequence encoding a complete protein, a protein fragment, a protein analogue, or a protein derivative each including the amino acid sequence represented by SEQ ID NO: 1. A cDNA, including the nucleotide sequence encoding the amino acid sequence represented by SEQ ID NO: 1.


Patent
CAS Institute of Zoology, CAS Institute of Genetics and Developmental Biology | Date: 2015-06-03

The present invention provides a method for nondestructive detection of a miRNA expression in a cell and determination of a cell type and state, and specifically provides a method for determining a cell type and state according to a miRNA expression in a cell culture medium. The method comprises: culturing cells of different types, collecting a culture medium of the cells, extracting RNA in the culture medium, performing inverse transcription on the RNA, detecting miRNA in the cell culture medium by using a fluorescent quantitative PCR method, and determining the type and state of the detected cell according to a relationship between miRNA expressions of different cell types and states and the detected miRNA expression. The method of the present invention proves for the first time that the miRNA expression in the culture medium can be detected to determine the type and state of a cell, comprising the pluripotency level of a stem cell and the state of a cell obtained through transdifferentiation, which avoids causing damages to the cell and is especially suitable for experimental and clinical applications where the number of cells is limited.


Both T-2 toxin and its metabolites are highly potent mycotoxins that can cause severe human and animal diseases upon exposure. Understanding the toxic mechanism and biotransformation process of T-2 toxin at a cellular level is essential for the development of counter-measures. We investigated the effect of T-2 toxin in porcine primary hepatocytes using porcine genome array and two-dimensional difference gel electrophoresis with matrix-assisted laser desorption/ionization tandem time of flight mass spectrometry. Integrated transcriptional and proteomic analysis demonstrated that T-2 toxin adversely affected porcine hepatocytes by initiating lipid metabolism disorder, oxidative stress response, and apoptosis. In addition, xenobiotic metabolism genes, including cytochrome P450 3As (CYP3A46 and CYP3A39), carboxylesterase 1Cs (CES1C4 and CES1C5), and epoxide hydrolase (EPHX1), increased in T-2 toxin treatment cells. Using HepG2 cells to over-express the recombinant xenobiotic metabolism genes above and rapid resolution liquid chromatography/tandem mass spectrometry to detect metabolites of T-2 toxin, we determined that porcine CYP3A46 mainly catalyzed T-2 to form 3'-hydroxy-T-2, which was further confirmed by purified CYP3A46 protein. However, recombinant porcine CES1C5 and EPHX1 did not enhance hydrolysis and de-epoxidation of T-2 implying that other esterases and epoxide hydrolases may play dominant roles in those reactions.


Zhao X.Y.,CAS Institute of Zoology
Nature protocols | Year: 2010

Induced pluripotent stem cells (iPSCs) are considered to be an attractive alternative to embryonic stem cells (ESCs) and may provide great potential for clinical applications in regenerative medicine. Although possessing characteristics similar to ESCs, the true pluripotency of these newly studied iPSCs was not known because none of the previously developed iPSCs passed the tetraploid complementation assay, which is regarded as the most stringent test for pluripotency. We have recently shown that by modifying some of the culture conditions for inducing iPSCs, we were able to generate cell lines of high pluripotency, resulting in the production of live-born, fertile animals through tetraploid complementation. In this paper, we describe details of our methods of generating iPS cell lines and subsequently producing full-term live animals through the tetraploid complementation assay; the procedure can be completed within 2 months.


Liu X.,CAS Institute of Zoology
Proceedings. Biological sciences / The Royal Society | Year: 2013

Global factors, such as climate change, international trade and introductions of exotic species are often elicited as contributors to the unprecedented rate of disease emergence, but few studies have partitioned these factors for global pandemics. Although contemporary correlative species distribution models (SDMs) can be useful for predicting the spatial patterns of emerging diseases, they focus mainly on the fundamental niche (FN) predictors (i.e. abiotic climate and habitat factors), neglecting dispersal and propagule pressure predictors (PP, number of non-native individuals released into a region). Using a validated, predictive and global SDM, we show that both FN and PP accounted for significant, unique variation to the distribution of the chytrid fungus Batrachochytrium dendrobatidis (Bd), a pathogen implicated in the declines and extinctions of over 200 amphibian species worldwide. Bd was associated positively with vegetation, total trade and introduced amphibian hosts, nonlinearly with annual temperature range and non-significantly with amphibian leg trade or amphibian species richness. These findings provide a rare example where both FN and PP factors are predictive of a global pandemic. Our model should help guide management of this deadly pathogen and the development of other globally predictive models for species invasions and pathogen emergence influenced by FN and PP factors.


Wang L.,CAS Institute of Zoology
Blood | Year: 2013

Reprogramming of somatic cells to desired cell types holds great promise in regenerative medicine. However, production of transplantable hematopoietic stem cells (HSCs) in vitro by defined factors has not yet been achieved. Therefore, it is critical to fully understand the molecular mechanisms of HSC development in vivo. Here, we show that Fev, an ETS transcription factor, is a pivotal regulator of HSC development in vertebrates. In fev-deficient zebrafish embryos, the first definitive HSC population was compromised and fewer T cells were found in the thymus. Genetic and chemical analyses support a mechanism whereby Fev regulates HSC through direct regulation of ERK signaling. Blastula transplant assay demonstrates that Fev regulation of HSC development is cell autonomous. Experiments performed with purified cord blood show that fev is expressed and functions in primitive HSCs in humans, indicating its conserved role in higher vertebrates. Our data indicate that Fev-ERK signaling is essential for hemogenic endothelium-based HSC development.


Wu J.J.,CAS Institute of Zoology
Proceedings. Biological sciences / The Royal Society | Year: 2013

The matrilineal Mosuo of southwest China live in large communal houses where brothers and sisters of three generations live together, and adult males walk to visit their wives only at night; hence males do not reside with their own offspring. This duolocal residence with 'walking' or 'visiting' marriage is described in only a handful of matrilineal peasant societies. Benefits to women of living with matrilineal kin, who cooperate with child-care, are clear. But why any kinship system can evolve where males invest more in their sister's offspring than their own is a puzzle for evolutionary anthropologists. Here, we present a new hypothesis for a matrilineal bias in male investment. We argue that, when household resources are communal, relatedness to the whole household matters more than relatedness to individual offspring. We use an inclusive fitness model to show that the more sisters (and other closely related females) co-reside, the more effort males should spend working on their sister's farm and less on their wife's farm. The model shows that paternity uncertainty may be a cause of lower overall work rates in males, but it is not likely to be the cause of a matrilineal bias. The bias in work effort towards working on their natal farm, and thus the duolocal residence and 'visiting marriage' system, can be understood as maximizing inclusive fitness in circumstances where female kin breed communally.


Patent
CAS Institute of Zoology | Date: 2015-08-19

The present invention provides an hnRNP A2* protein, nucleic acid for coding the protein and a use thereof. The protein contains a protein, a protein segment or a protein analog or derivative having an amino acid sequence shown as SEQ ID NO. 1. The hnRNP A2* protein can actively unloosen telomere G-quadruplexes, bind the minimum binding site at the 3 tail end of telomere, expose five nucleotide tails which can be matched with a telomere enzyme RNA template and promote the extension of telomere under the effect of telomere enzyme, thereby maintaining the cell division potential.


An hnRNP A2* protein, including an amino acid sequence represented by SEQ ID NO. 1. The protein is a complete protein, a protein fragment, a protein analogue or a protein derivative.

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