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Liu C.,CAS Institute of Electronics
Microfluidics and Nanofluidics | Year: 2010

This article describes a simple and rapid fabrication method for microfluidic chip with three-dimensional (3D) structures by using natural lotus leaf as a template. This microfluidic chip consists of one PDMS artificial lotus leaf substrate with micro/nano hierarchical structure and one PDMS replica with microfluidic network. PDMS artificial lotus leaf substrate is directly replicated from natural lotus leaf template by soft lithography technology. In order to seal this rough PDMS artificial lotus leaf substrate against PDMS replica, a PDMS adhesiveassisted bonding method is developed to assist the sealing of our microfluidic chip. Compared to previously reported microfluidic chips with 3D structures based on MES/ NEMS technology, the rapid fabrication of our microfluidic chip against a natural lotus leaf template is simple, low cost, easy to control, and highly faithful without the need of expensive MEMS facility and clean room condition. As an application demonstration, we fabricate a microfluidic immunosensing chip for C-reactive protein (CRP) detection by taking advantage of its high surface area. The experiment result shows that our microfluidic chip with 3D structures has more than five times higher sensitivity than those obtained from the conventional microfluidic chip with a flat channel wall due to the increase of the surface area and consequent increase of the amount of immobilized antibody. The present microfluidic chip with 3D structures also can be applied in proteins microarray, cell culture, cell adhesion, and so on. Source


Chen J.,CAS Institute of Electronics | Li J.,University of Toronto | Sun Y.,University of Toronto
Lab on a Chip - Miniaturisation for Chemistry and Biology | Year: 2012

This article reviews the recent developments in microfluidic technologies for in vitro cancer diagnosis. We summarize the working principles and experimental results of key microfluidic platforms for cancer cell detection, characterization, and separation based on cell-affinity micro-chromatography, magnetic activated micro-sorting, and cellular biophysics (e.g., cell size and mechanical and electrical properties). We examine the advantages and limitations of each technique and discuss future research opportunities for improving device throughput and purity, and for enabling on-chip analysis of captured cancer cells. This journal is © 2012 The Royal Society of Chemistry. Source


Provided are a microscopic surgery system and a navigation method guided by optical coherence tomography. The microscopic surgery system may include: an object lens; a surgical microscope unit configured to perform two-dimensional imaging on an area to operate on via the object lens; an optical coherence tomography unit configured to perform two-dimensional tomography imaging on the area via the object lens, with an imaging field calibrated according to that of the surgical microscope unit; a processor configured to obtain navigation information based on the two-dimensional imaging by the surgical microscope unit and the two-dimensional tomography imaging by the optical coherence tomography unit, the navigation information comprising positional information of a body part to operate on and a surgical instrument for operating on the body part; and an output unit configured to output the navigation information to guide the surgical instrument to the body part.


The present disclosure provides detection devices and methods using a diffraction wavefront of a pinhole stitching measurement of surface shape. The light emitted from the laser passes through a filter hole, a first condenser lens, a spatial filter, a beam expander, a half wave plate, a /4 wave plate, an attention plate and then is transmitted through a beam splitter, reflected by a reflecting mirror and is irradiated onto an pinhole through a first optical adjustable shelf and a second set of condenser lens. A part of diffraction light generated by the pinhole is irradiated to the mirror to be measured; the light reflected by the mirror to be measured is reflected by a frame of the pinhole and generate a diffraction fringe along with another part of the diffraction wavefront of the pinhole. The interference fringe is focused by the third set of condenser lens on the third optical adjustable shelf and is collected by the CCD detector. The mirror to be measured is positioned on the second optical adjustable shelf and may be moved along a normal direction of the mirror to be measured to implement an annular aperture stitching measurement. Meanwhile, the first optical adjustable shelf may be rotated and moved in translation to measure the mirror by a scanning sub apertures stitching measurement.


Patent
Shenzhen Mindray Bio Medical Electronics Co. and CAS Institute of Electronics | Date: 2015-02-10

A flow cytometer and a fluid system are provided. The fluid system comprises a flow cell, a sample providing unit, a waste container, a sheath container, a negative pressure source, a quantitative unit and a sample flow monitoring unit, the negative pressure source, the waste container and the flow cell are connected, a negative pressure source, which provides a negative pressure relative to the sample providing unit for the flow cell so that the sample providing unit causes the sample to flow into the flow cell under the negative pressure, a sample flow monitoring unit monitors a flow of the sample fluid transported from the sample providing unit to the flow cell and outputs a feedback signal reflecting flow changes of the sample fluid in real-time; wherein the controller receives the feedback signal and controls the quantitative unit to adjust a flow of the sheath fluid according to the feedback signal.

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