Liu F.,CAS East China Sea Fisheries Research Institute |
Pang S.,CAS Qingdao Institute of Oceanology
Mitochondrial DNA | Year: 2014
Sargassum muticum (Yendo) Fensholt is an invasive canopy-forming brown alga, expanding its presence from Northeast Asia to North America and Europe. The complete mitochondrial genome of S. muticum is characterized as a circular molecule of 34,720 bp. The overall AT content of S. muticum mitogenome is 63.41%. This mitogenome contains 65 genes typically found in brown algae, including 3 ribosomal RNA genes, 25 transfer RNA genes, 35 protein-coding genes, and 2 conserved open reading frames (ORFs). The gene order of mitogenome for S. muticum is identical to that for Sargassum horneri, Fucus vesiculosus and Desmarestia viridis. Phylogenetic analyses based on 35 protein-coding genes reveal that S. muticum has a close evolutionary relationship with S. horneri and a distant relationship with Dictyota dichotoma, supporting current taxonomic systems. The present investigation provides new molecular data for studies of S. muticum population diversity as well as comparative genomics in the Phaeophyceae. © 2014 Informa UK Ltd. All rights reserved: reproduction in whole or part not permitted
Ma Z.,Xiamen University |
Ma Z.,CAS East China Sea Fisheries Research Institute |
Gao K.,Xiamen University
Environmental and Experimental Botany | Year: 2010
Previous studies showed that exposure of Arthrospira spp. spirals to natural levels of solar radiation in the presence of UV radiation (UVR, 280-400 nm) led to the breakage of its spiral structure. However, the underlying mechanisms have not yet been explored. Here, we showed that associated accumulation of reactive oxygen species (ROS) resulted in the spiral breakage by oxidizing the lipids of sheath or cell membrane in Arthospira platensis, and presence of UVR brought about higher accumulation level of the ROS. Activities of superoxide dismutase (SOD) and catalase (CAT) were inhibited by high levels of solar PAR, addition of UVR led to further inhibition of CAT activity. High levels of ROS also decreased the content of photosynthetic pigments, damaged photosystem II (PSII) and inhibited the photosynthesis and growth. It is concluded that both UV and high PAR levels could generate higher amounts of ROS, which decreased the photosynthetic performances and led to spiral breakage of A. platensis. © 2009 Elsevier B.V. All rights reserved.
Gao K.,Xiamen University |
Zheng Y.,CAS East China Sea Fisheries Research Institute
Global Change Biology | Year: 2010
Previous studies have shown that increasing atmospheric CO 2 concentrations affect calcification in some planktonic and macroalgal calcifiers due to the changed carbonate chemistry of seawater. However, little is known regarding how calcifying algae respond to solar UV radiation (UVR, UVA+UVB, 280-400 nm). UVR may act synergistically, antagonistically or independently with ocean acidification (high CO 2/low pH of seawater) to affect their calcification processes. We cultured the articulated coralline alga Corallina sessilis Yendo at 380 ppmv (low) and 1000 ppmv (high) CO 2 levels while exposing the alga to solar radiation treatments with or without UVR. The presence of UVR inhibited the growth, photosynthetic O 2 evolution and calcification rates by13%, 6% and 3% in the low and by 47%, 20% and 8% in the high CO 2 concentrations, respectively, reflecting a synergistic effect of CO 2 enrichment with UVR. UVR induced significant decline of pH in the CO 2-enriched cultures. The contents of key photosynthetic pigments, chlorophyll a and phycobiliproteins decreased, while UV-absorptivity increased under the high pCO 2/low pH condition. Nevertheless, UV-induced inhibition of photosynthesis increased when the ratio of particulate inorganic carbon/particulate organic carbon decreased under the influence of CO 2-acidified seawater, suggesting that the calcified layer played a UV-protective role. Both UVA and UVB negatively impacted photosynthesis and calcification, but the inhibition caused by UVB was about 2.5-2.6 times that caused by UVA. The results imply that coralline algae suffer from more damage caused by UVB as they calcify less and less with progressing ocean acidification. © 2009 Blackwell Publishing Ltd.
Luo M.B.,CAS East China Sea Fisheries Research Institute |
Liu F.,CAS Qingdao Institute of Oceanology
Journal of Experimental Marine Biology and Ecology | Year: 2011
The physiological and biochemical responses to salinity stresses in Ulva prolifera were investigated, including growth rate, level of oxidative stress and regulation of antioxidant defense system. A six-day exposure to hyposaline (10‰) and hypersaline (60‰) conditions resulted in a significant decrease in growth rate and maximum photosynthetic quantum efficiency (Fv/Fm) compared with the control (30‰). Increases in H2O2 contents correlated to the level of lipid peroxidation, which suggested that oxidative damage occurred in salinity stress and was more severe at 60‰ than at 10‰. The amount of total soluble protein (TSP) significantly increased in a hypersaline condition. The fluctuations of four antioxidant substrates and four antioxidant enzymes were determined after the long-term salinity stress. Compared to growth at 30‰, low salinities led to a major increase in activities of catalase (CAT), superoxide dismutase (SOD) and glutathione reductase (GR), coupled with an increase in contents of ascorbate, glutathione and β-carotenoid. Thalli exposed to hypersaline conditions rapidly accumulated glutathione and did not affect the content of ascorbate, α-tocopherol and β-carotenoid. The activities of CAT, SOD, ascorbate peroxidase (APX) and GR increased in hypersaline conditions, suggesting that reactive oxygen scavenging enzymes played an important role in U. prolifera for adapting to the hypersaline condition. The alterations in antioxidant enzymes and substrates are not consistent between hyposaline and hypersaline stresses in U. prolifera, but the regulation of antioxidant defense system was a vital tolerance mechanism involved in the oxidative stress. © 2011 Elsevier B.V.
Luo M.B.,CAS East China Sea Fisheries Research Institute |
Liu F.,CAS Qingdao Institute of Oceanology |
Xu Z.L.,CAS East China Sea Fisheries Research Institute
Aquatic Botany | Year: 2012
This study was designed to evaluate interspecific physiological differences in growth and nutrient uptake of two co-occurring species, Ulva prolifera and Ulva linza, in laboratory conditions. Growth-testing results showed that U. prolifera grew faster than U. linza in a range of temperature (8-30°C) and irradiance (50-500μmol photon m -2s -1). Nitrate (NO 3 -) and ammonium (NH 4 +) enrichment had far more effect on growth of U. prolifera (max. 13.1 and 16.9%d -1) than on U. linza (max. 9.8 and 11.6%d -1). The V max values of NO 3 - and NH 4 + uptake by U. prolifera (124.25 and 284.60μmolg -1DMh -1) were higher than those by U. linza (109.13 and 250.25μmolg -1DMh -1), and the K s values for U. prolifera were much lower than those for U. linza during the 3-h testing period. Significant difference in the V max/K s values indicated that U. prolifera had an apparent competitive advantage over U. linza to uptake and stock nitrogen (NO 3 - and NH 4 +) from the water column. There was no distinct difference in phosphate uptake between the two species. The results of this investigation revealed that distinct interspecific physiological traits in growth and nutrient uptake might be key factors to determine the species dominance patterns in green tides in the Yellow Sea. © 2012.
Li Z.,Zhejiang Pharmaceutical College |
Gao Q.,CAS East China Sea Fisheries Research Institute
Animal Cells and Systems | Year: 2013
With increasing use of natural plant-derived components, exploring the anti-proliferative effects of phytochemicals is increasingly gaining importance in designing anticancer drugs. Quercetin, a natural constituent abundantly present in food products, is capable of inducing apoptosis in tumor cells. However, little is known about its biological effect on colon cancer cells and molecular mechanism leading to this event. The aim of this study was to investigate the effect of quercetin on the apoptotic pathway in human colon carcinoma cells (HT-29 cells). The results indicated that quercetin induced suppression of cell viability and apoptosis in HT-29 cells in a dose-dependent manner. This involved characteristic changes in nuclear morphology, activation of caspases-3 and caspases-9, collapse of Mitochondrial membrane potential (ΔΨm), upregulation of pro-apoptotic Bax, and downregulation of anti-apoptotic Bcl-2 and Bcl-XL. Quercetin, which exerts anti-proliferative effect though different signaling pathways, is a classic candidate for anti-colon cancer drug design. © 2013 Copyright Korean Society for Integrative Biology.
Zhang H.,CAS East China Sea Fisheries Research Institute
Journal of Applied Ichthyology | Year: 2013
Summary: The structure and temporal variations of the fish community in the intertidal estuarine zone of shallow mud areas have been poorly studied in China. This paper analyses the diel, semi-lunar and seasonal patterns of fish assemblages in the Yangtze estuary in 2006. Fish were collected by consecutive day and night samplings using tide-stow-nets deployed parallel to each other in three stations. A total of 56 fish species belonging to 21 families was caught during the study period. The family Cyprinidae dominated with 25 species. Freshwater fish species were the important dominant commercial fishery species and well represented with five species (sharpbelly Hemiculter bleekeri, goldfish Carassius auratus, bream Parabramis pekinensis, likely-bream Pseudobrama simony, and glossy yellow catfish Pelteobagrus nitidus) in the three stations. Juvenile fishes dominated the fish community, comprising 93.9% in station 1 and 96.6% in station 2 of the total abundance. The number of fish species in day tides was slightly lower than those in night tides in spring and summer, but the opposite in other seasons. In neap tides, the numbers and abundance of fish species were both lower than those in the spring tides. Fish abundance was lowest in winter, increasing during spring and summer (March-September) in both stations 1 and 2, with obviously large fluctuations in each season. The pattern of habitat selection of fishes could effectively decrease the food competition of intraspecies or interspecies and favour the growth and nursing of young fishes. These findings indicate that the intertidal zones in the estuary may serve as important nursery areas for fish communities. © 2013 Blackwell Verlag GmbH.
Yang Q.,CAS East China Sea Fisheries Research Institute
Applied Mechanics and Materials | Year: 2013
The marine symbiotic bacteria strains were isolated from the Antarctic krill (Euphausia superb) samples captured in Antarctica Ocean. The taxonomic identification was then performed. The screening of the bioactivity against nitric oxide (NO) release for the crude extract after the strain fermentation and cell culture extraction were finally carried out. The taxonomic identification analysis showed the strain was very close to Salegentibacter salinus ISL with the similarity value of 94.5% based on 16S rDNA gene sequencing and homology analysis. According to the analysis, this strain was identified as a new strain and nominated as Salegentibacter sp. NJ-102. The bioactivity screening analysis of the culture extract demonstrated the crude extract showed significant inhibitory bioactivity against NO release and the expression of the inducible nitric oxide synthase (iNOS) in the ECV-304 cells. © (2013) Trans Tech Publications, Switzerland.
Li C.,University of Nebraska - Lincoln |
Riethoven J.-J.M.,University of Nebraska - Lincoln |
Riethoven J.-J.M.,CAS East China Sea Fisheries Research Institute
BMC Evolutionary Biology | Year: 2010
Background: Exon-primed intron-crossing (EPIC) markers have three advantages over anonymous genomic sequences in studying evolution of natural populations. First, the universal primers designed in exon regions can be applied across a broad taxonomic range. Second, the homology of EPIC-amplified sequences can be easily determined by comparing either their exon or intron portion depending on the genetic distance between the taxa. Third, having both the exon and intron fragments could help in examining genetic variation at the intraspecific and interspecific level simultaneously, particularly helpful when studying species complex. However, the paucity of EPIC markers has hindered multilocus studies using nuclear gene sequences, particularly in teleost fishes. Results. We introduce a bioinformatics pipeline for developing EPIC markers by comparing the whole genome sequences between two or more species. By applying this approach on five teleost fishes whose genomes were available in the Ensembl database http://www.ensembl.org, we identified 210 EPIC markers that have single-copy and conserved exon regions with identity greater than 85% among the five teleost fishes. We tested 12 randomly chosen EPIC markers in nine teleost species having a wide phylogenetic range. The success rate of amplifying and sequencing those markers varied from 44% to 100% in different species. We analyzed the exon sequences of the 12 EPIC markers from 13 teleosts. The resulting phylogeny contains many traditionally well-supported clades, indicating the usefulness of the exon portion of EPIC markers in reconstructing species phylogeny, in addition to the value of the intron portion of EPIC markers in interrogating the population history. Conclusions. This study illustrated an effective approach to develop EPIC markers in a taxonomic group, where two or more genome sequences are available. The markers identified could be amplified across a broad taxonomic range of teleost fishes. The phylogenetic utility of individual markers varied according to intron size and amplifiability. The bioinformatics pipelines developed are readily adapted to other taxonomic groups. © 2010 Li et al; licensee BioMed Central Ltd.
Xu Z.L.,CAS East China Sea Fisheries Research Institute
Plankton and Benthos Research | Year: 2010
Distribution patterns and abundance of the epiplanktonic shrimp Lucifer were examined in the East China Sea (23°30'-33°N, 118°30'-128°E), in relation to temperature and salinity. A total of 443 samples were collected from four seasonal surveys conducted between 1997 and 2000. The yield density model was used to predict optimal temperature (OT) and optimal salinity (OS) of four Lucifer species: Lucifer typus, L. hanseni, L. intermedius and L. penicillifer. Thereafter, their distribution patterns were determined. The results indicated that these species are the most abundant in summer. Lucifer typus, with OT of 28.0°C and OS of 33.8, is considered to be an oceanic tropic water species. The species is mainly found in the northern waters off Taiwan in summer and autumn. Lucifer hanseni, L. intermedius and L. penicillifer, with OTs of 26.4, 28.0, 27.4°C and OSs of 33.6, 33.4, 33.2, are off-shore subtropical water species. They are mainly distributed in the south off-shore the East China Sea and north of Taiwan. © The Plankton Society of Japan.