Entity

Time filter

Source Type


Schwudke D.,MPI of Molecular Cell Biology and Genetics | Schwudke D.,Tata Institute of Fundamental Research | Schuhmann K.,MPI of Molecular Cell Biology and Genetics | Schuhmann K.,Carl Gustav Carus Clinics of Dresden University of Technology | And 4 more authors.
Cold Spring Harbor Perspectives in Biology | Year: 2011

Despite their compositional complexity, lipidomes comprise a large number of isobaric species that cannot be distinguished by conventional low resolution mass spectrometry and therefore in-depth MS/MS analysis was required for their accurate quantification. Here we argue that the progress in high resolution mass spectrometry is changing the concept of lipidome characterization. Because exact masses of isobaric species belonging to different lipid classes are not necessarily identical, they can now be distinguished and directly quantified in total lipid extracts. By streamlining and simplifying the molecular characterization of lipidomes, high resolution mass spectrometry has developed into a generic tool for cell biology and molecular medicine. © 2011 Cold Spring Harbor Laboratory Press. Source


Herzog R.,Max Planck Institute of Molecular Cell Biology and Genetics | Herzog R.,Carl Gustav Carus Clinics of Dresden University of Technology | Schwudke D.,Max Planck Institute of Molecular Cell Biology and Genetics | Schwudke D.,Tata Institute of Fundamental Research | And 6 more authors.
Genome Biology | Year: 2011

Shotgun lipidome profiling relies on direct mass spectrometric analysis of total lipid extracts from cells, tissues or organisms and is a powerful tool to elucidate the molecular composition of lipidomes. We present a novel informatics concept of the molecular fragmentation query language implemented within the LipidXplorer open source software kit that supports accurate quantification of individual species of any ionizable lipid class in shotgun spectra acquired on any mass spectrometry platform. © 2011 Herzog et al.; licensee BioMed Central Ltd. Source


Herzog R.,Max Planck Institute of Molecular Cell Biology and Genetics | Herzog R.,Carl Gustav Carus Clinics of Dresden University of Technology | Schuhmann K.,Max Planck Institute of Molecular Cell Biology and Genetics | Schuhmann K.,Carl Gustav Carus Clinics of Dresden University of Technology | And 6 more authors.
PLoS ONE | Year: 2012

LipidXplorer is the open source software that supports the quantitative characterization of complex lipidomes by interpreting large datasets of shotgun mass spectra. LipidXplorer processes spectra acquired on any type of tandem mass spectrometers; it identifies and quantifies molecular species of any ionizable lipid class by considering any known or assumed molecular fragmentation pathway independently of any resource of reference mass spectra. It also supports any shotgun profiling routine, from high throughput top-down screening for molecular diagnostic and biomarker discovery to the targeted absolute quantification of low abundant lipid species. Full documentation on installation and operation of LipidXplorer, including tutorial, collection of spectra interpretation scripts, FAQ and user forum are available through the wiki site at: https://wiki.mpi-cbg.de/wiki/lipidx/index.php/Main_Page. © 2012 Herzog et al. Source

Discover hidden collaborations