Ishibashi Y.,Cardiovascular Laboratory |
Nishigami K.,Saiseikai Kumamoto Hospital |
Watanabe M.,Cardiovascular Laboratory |
Takaji K.,Saiseikai Kumamoto Hospital |
Uesugi H.,Saiseikai Kumamoto Hospital
Journal of Echocardiography | Year: 2013
A 76-year-old woman presented with shortness of breath and dyspnea after the intake of meals. Chest X-ray showed pulmonary congestion and pleural effusion. Computed tomography disclosed a hiatus hernia. Echocardiography demonstrated that the motion of the posterior wall in the left ventricle (LV) was paradoxically by the hiatus hernia, although LV ejection fraction was preserved. The restriction of LV by hiatus hernia could cause heart failure and open surgical repair of the hiatus hernia was performed. Dyspnea after the intake of meals disappeared and no recurrence of heart failure was observed in the subsequent period of several years. © 2013 Japanese Society of Echocardiography.
Li J.,Cardiovascular Laboratory |
Yang G.,Cardiovascular Laboratory |
Feng M.,Sun Yat Sen University |
Liang H.,Cardiovascular Laboratory |
And 4 more authors.
Biotechnology and Bioprocess Engineering | Year: 2012
The major limitations to non-viral gene deliveiy are relatively low efficiency and cytotoxicity, which need to be addressed in the design of new vectors. In this study, negatively charged low density lipoproteins (LDL) were coated onto positively charged pVEGF/PEI complexes to form pVEGF/PEI/LDL terplexes by a two-step procedure. The biocompatible LDL was introduced to reduce the cytotoxicity of the gene delivery system and increase its affinity to cells. The successful formation of pVEGF/PEI/LDL terplexes was confirmed by their near-neutral and slightly negative surface charges. The pVEGF/PEI/LDL terplexes were well-defined sub-micron spherical particles. On the cell viability assay, both of the PEI/LDL combined vector and pVEGF/PEI/LDL terplexes exhibited much lower cytotoxicity to HeLa cells and HUVE cells than those of PEI and pVEGF/PEI complexes, attributed to the shielding effect of the LDL. pEGFP/PEI/LDL terplexes showed significantly higher transfection efficiency in comparison to pEGFP/PEI complexes in serum-containing medium. pVEGF/PEI/LDL terplexes at their optimal N/P ratio and LDL/PEI weigh ratio induced higher expression levels of VEGF protein in HUVE cells than those of pVEGF/PEI complexes. Therefore, the pVEGF/PEI/LDL terplexes could be used as a promising gene delivery system to enhance VEGF protein expression. © The Korean Society for Biotechnology and Bioengineering and Springer 2012.