Cardiovascular Engineering Center CEC

Malaysia

Cardiovascular Engineering Center CEC

Malaysia
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Ishak S.A.,Cardiovascular Engineering Center CEC | Sukmana I.,Cardiovascular Engineering Center CEC | Sukmana I.,Lampung University
Advanced Materials Research | Year: 2014

Angiogenesis is the formation of new microvascular network from the pre-existing blood vessel. In tissue engineering approaches, angiogenesis is essential for the promotion of micro-vascular network inside an engineered scaffold construct, mimicking a functional blood vessel in vivo. In the in vivo system, the formation of new blood vessels depends on the properties fibrin gel extracellular matrix. In this study, we have investigated the effect of different fibrinogen and thrombin composition on the biophysical properties of fibrin gel. Higher concentration of thrombin (4.0 Units/milliliter) yields a shorter clotting time of the fibrin gel and result in better water uptake property while at lower concentration of thrombin (0.5 Units/milliliter), the clotting time takes much longer. Also, at lowest concentration ratio of fibrinogen to thrombin (0.5 milligram/milliliter to 4.0 Units/milliliter), the turbidity study shows the lowest absorbance compared to other samples. Different concentration of fibrinogen and thrombin also affect the microstructure of the fibrin gel. The variation of these properties will be then manipulated to be used for in vitro angiogenesis. This study opens broader application of fibrin extracellular matrix in regenerative medicine and tissue engineering researches. © (2014) Trans Tech Publications, Switzerland.


Ishak S.A.,Cardiovascular Engineering Center CEC | Djuansjah J.R.P.,University of Technology Malaysia | Kadir M.R.A.,Cardiovascular Engineering Center CEC | Sukmana I.,Cardiovascular Engineering Center CEC | Sukmana I.,Lampung University
IOP Conference Series: Materials Science and Engineering | Year: 2014

Angiogenesis, the formation of micro-vascular network from the preexisting vascular vessels, has been studied in the connection to the normal developmental process as well as numerous diseases. In tissue engineering research, angiogenesis is also essential to promote micro-vascular network inside engineered tissue constructs, mimicking a functional blood vessel in vivo. Micro-vascular network can be used to maintain adequate tissue oxygenation, nutrient transfer and waste removal. One of the problems faced by angiogenesis researchers is to find suitable in vitro assays and methods for assessing the effect of regulators on angiogenesis and micro-vessel formation. The assay would be reliable and repeatable with easily quantifiable with physiologically relevant. This review aims to highlights recent advanced and future challenges in developing and using an in vitro angiogenesis assay for the application on biomedical and tissue engineering research. © Published under licence by IOP Publishing Ltd.

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