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Alexandroúpoli, Greece

Ragia G.,Democritus University of Thrace | Nikolaidis E.,Cardiothoracic Surgery Clinic | Tavridou A.,Democritus University of Thrace | Arvanitidis K.I.,Democritus University of Thrace | And 4 more authors.
JRAAS - Journal of the Renin-Angiotensin-Aldosterone System | Year: 2010

Introduction. Candidates for coronary artery bypass grafting (CABG) represent a group of patients with well documented, severe coronary artery disease (CAD). Genetic polymorphisms of renin-angiotensin-aldosterone system (RAAS) components have been associated with CAD. We examined the association of polymorphisms of angiotensin-converting enzyme (ACE), angiotensinogen (AGT), and angiotensin II type 1 receptor (AT1 receptor) with severe CAD in CABG patients. Materials and methods. One hundred and fifty-four CABG patients and 155 non-CAD controls were included in the study. Established PCR methods were used for genotyping of AGT M235T, AGT T174M, AT1 receptor A1166C, and ACE I/D polymorphisms. Cumulative effect of analysed polymorphisms was assessed by calculation of each individualĝ€™s RAAS gene score (addition of 0.5 points for each variant allele and then calculating the sum for all four polymorphisms). Results. No association between AGT M235T, AGT T174M, ACE I/D and AT1 receptor A1166C polymorphisms and CAD was observed. Within CABG patients, the frequency of homozygous AGT 235TT genotype was higher in hypertensive compared to normotensive CABG patients (21.7% vs. 6.3%, p=0.03). RAAS gene score did not differ between CABG patients and non-CAD controls. Conclusions. There is no association of the analysed RAAS polymorphisms with severe CAD in CABG patients. However, within these patients, an association was found between AGT 235TT genotype and hypertension. © 2010 The Authors.


Ragia G.,Democritus University of Thrace | Nikolaidis E.,Cardiothoracic Surgery Clinic | Tavridou A.,Democritus University of Thrace | Arvanitidis K.I.,Democritus University of Thrace | And 4 more authors.
Human Genomics | Year: 2016

Polymorphisms in the endothelial nitric oxide synthase (eNOS) gene (-786T >C and 894G >T) enhance endo-thelial dysfunction and have been studied in relation to coronary artery disease (CAD). In the present study, we examined the association of the above polymorphisms with CAD, as well as with myocardial infarction (MI), hypertension, diabetes and smoking in CAD patients. Study subjects consisted of 154 consecutive coronary artery bypass graft (CABG) patients and 155 non-CAD controls. eNOS -786T >C and 894G >T polymorphisms were genotyped by polymerase chain reaction-restriction fragment length polymorphism. The estimated frequencies of the -786C and 894T alleles did not differ between the two groups (p = 0.46 and p = 0.84, respectively). The prevalence of eNOS polymorphisms was not associated with MI, hypertension or diabetes in CABG patients; however, we found that the 894TT genotype and 894T allele were significantly more frequent in current/past smoker CABG patients (16.7 per cent and 39.6 per cent, respectively) compared with never smoker CABG patients (6.1 per cent and 24.4 per cent, respectively) (p = 0.01 and p < 0.01, respectively). We found no association of eNOS -786C and 894T variant alleles with CAD; however, within CABG patients, a gene-environment interaction was found between the eNOS 894T allele and smoking. © 2010 Henry Stewart Publications.

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