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Saraswathy A.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS | Suganthan J.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS | Ariyanathan S.,SASTRA University | Hemakumar N.S.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS
International Journal of Pharmacy and Technology | Year: 2011

The plant Plumbago zeylanica Linn. of Plumbaginaceae known as Chitraka/ Chitramoolam is a popular drug in Ayurveda and Siddha. The root of this plant finds place in several of the compound formulations of these systems of medicine, as one of the ingredients. The root being a vital component in these formulations, its quality and consistency is of prime importance. Plumbagin being the major bioactive chemical was used as a reference standard with which the HPTLC chromatogram obtained for each sample was compared. Plumbagin present in each sample was quantified using a calibration graph of the reference standard. The present study deals with the study of the presence of any variation in the plumbagin content due to change in season and storage. Sufficient quantities of root was collected during the month of July 2008 and stored at room temperature for the assay of Plumbagin. From August onwards every month, fresh root samples were also collected till June 2009. Simultaneous comparison of the fingerprints of n-hexane extract of the roots as well as estimation of plumbagin was done on the samples collected and the stored sample at room temperature using HPTLC technique. The plumbagin content showed a sharp decline from July 2008 to June 2009 on the stored root samples. The study established more concentration of Plumbagin constituent in February followed by January. The content was found to be very less in April. Hence the root may show more efficacy if collected in February. The technique is simple and cost effective. Source


Saraswathy A.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS | Vidhya B.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS
International Journal of Pharmacy and Technology | Year: 2013

Objective: To establish the fingerprint profile for Hemidesmus indicus (Linn.) R. Br. roots using high performance thin layer chromatography (HPTLC) technique for quality control. Methods: CAMAG HPTLC system equipped with TLC auto sampler 4 applicator, TLC scanner 3 and win CATS 1.4.4. software was used. Chloroform and ethyl alcohol extracts of the roots were developed in suitable mobile phase using standard procedures and scanned under UV at 254 nm, 366 nm and under visible light. Results: The HPTLC fingerprinting of the chloroform and ethyl alcohol extracts showed several peaks with different Rf values. Conclusion: HPTLC fingerprint profile of chloroform extract of authentic sample of Hemidesmus indicus may be useful in the identification and authentication of the plant. Source


Arirudran B.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS | krishnamurthy V.,Bharathi Womens College | Saraswathy A.,Captain Srinivasa Murti Drug Research Institute for Ayurveda CCRAS
Journal of Applied Pharmaceutical Science | Year: 2014

The present attempt has been made to evaluate, and examine the levels of minerals in serum and liver in DEN induced hepatocellular carcinoma in wistar albino rats for possible chemopreventive effect. In hepatocellular carcinogenesis complications such as hepatic fibrosis and cirrhosis may lead to several abnormalities in mineral metabolism, hence attempt is made to evaluate on the level of minerals. Hepatic cancer was induced by single dose of intraperitoneal injections of DEN (200mg/kg body weight) followed by phenobarbital of 0.05% mixed with drinking water for 20 weeks. Concentration of calcium, magnesium, sodium and potassium were assessed in the serum and liver at the end of experimental period. Negative correlations were observed between liver function tests and serum mineral levels, except with albumin. Calcium, magnesium, potassium and sodium concentrations in the serum were decreased after the induction of hepatic cancer. The liver calcium content was increased after DEN treatment. No change occurred in liver sodium content. However, magnesium and potassium content was significantly reduced in the hepatic tissue. The results suggest that in DEN-induced hepato cellular carcinoma alteration of essential elements is noted. The low levels of albumin and the related ascites may be one of the major causes of the imbalance of mineral metabolism in hepatocellular carcinoma. © 2014 B Arirudran et al. Source

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