CapSenze HB

Billeberga, Sweden

CapSenze HB

Billeberga, Sweden

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Lenain P.,Ghent University | De Saeger S.,Ghent University | Mattiasson B.,Capsenze HB | Hedstrom M.,Capsenze HB
Biosensors and Bioelectronics | Year: 2015

An affinity sensor based on capacitive transduction was developed to detect a model compound, metergoline, in a continuous flow system. This system simulates the monitoring of low-molecular weight organic compounds in natural flowing waters, i.e. rivers and streams. During operation in such scenarios, control of the experimental parameters is not possible, which poses a true analytical challenge. A two-step approach was used to produce a sensor for metergoline. Submicron spherical molecularly imprinted polymers, used as recognition elements, were obtained through emulsion polymerization and subsequently coupled to the sensor surface by electropolymerization. This way, a robust and reusable sensor was obtained that regenerated spontaneously under the natural conditions in a river. Small organic compounds could be analyzed in water without manipulating the binding or regeneration conditions, thereby offering a viable tool for on-site application. © 2015 Elsevier B.V.


PubMed | Capsenze HB and Ghent University
Type: | Journal: Biosensors & bioelectronics | Year: 2015

An affinity sensor based on capacitive transduction was developed to detect a model compound, metergoline, in a continuous flow system. This system simulates the monitoring of low-molecular weight organic compounds in natural flowing waters, i.e. rivers and streams. During operation in such scenarios, control of the experimental parameters is not possible, which poses a true analytical challenge. A two-step approach was used to produce a sensor for metergoline. Submicron spherical molecularly imprinted polymers, used as recognition elements, were obtained through emulsion polymerization and subsequently coupled to the sensor surface by electropolymerization. This way, a robust and reusable sensor was obtained that regenerated spontaneously under the natural conditions in a river. Small organic compounds could be analyzed in water without manipulating the binding or regeneration conditions, thereby offering a viable tool for on-site application.


Erturk G.,Lund University | Erturk G.,Hacettepe University | Hedstrom M.,Lund University | Hedstrom M.,CapSenze HB | And 4 more authors.
Analytica Chimica Acta | Year: 2015

Prostate specific antigen (PSA) is a valuable biomarker for early detection of prostate cancer, the third most common cancer in men. Ultrasensitive detection of PSA is crucial to screen the prostate cancer in an early stage and to detect the recurrence of the disease after treatment. In this report, microcontact-PSA imprinted (PSA-MIP) capacitive biosensor chip was developed for real-time, highly sensitive and selective detection of PSA. PSA-MIP electrodes were prepared in the presence of methacrylic acid (MAA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker via UV polymerization. Immobilized Anti-PSA antibodies on electrodes (Anti-PSA) for capacitance measurements were also prepared to compare the detection performances of both methods. The electrodes were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and cyclic voltammetry (CV) and real-time PSA detection was performed with standard PSA solutions in the concentration range of 10 fg mL-1-100 ng mL-1. The detection limits were found as 8.0 × 10-5 ng mL-1 (16 × 10-17 M) and 6.0 × 10-4 ng mL-1 (12 × 10-16 M) for PSA-MIP and Anti-PSA electrodes, respectively. Selectivity studies were performed against HSA and IgG and selectivity coefficients were calculated. PSA detection was also carried out from diluted human serum samples and finally, reproducibility of the electrodes was tested. The results are promising and show that when the sensitivity of the capacitive system is combined with the selectivity and reproducibility of the microcontact-imprinting procedure, the resulting system might be used successfully for real-time detection of various analytes even in very low concentrations. © 2015 Elsevier B.V.


Erturk G.,Lund University | Erturk G.,Hacettepe University | Berillo D.,Lund University | Hedstrom M.,Lund University | And 3 more authors.
Biotechnology Reports | Year: 2014

An analytical method is presented, combining novel microcontact imprinting technique and capacitive biosensor technology for the detection of BSA. Glass cover slips were used for preparation of protein stamps. The microcontact-BSA imprinted gold electrodes were prepared in the presence of methacrylic acid (MAA) and poly-ethylene glycol dimethacrylate (PEGDMA) as the cross-linker by bringing the protein stamp and the gold electrode into contact under UV-polymerization. Real-time BSA detection studies were performed in the concentration range of 1.0 × 10-20-1.0 × 10-8 M with a limit of detection (LOD) of 1.0 × 10-19 M. Cross-reactivity towards HSA and IgG were 5 and 3%, respectively. The electrodes were used for >70 assays during 2 months and retained their binding properties during all that time. The NIP (non-imprinted) electrode was used as a reference. The microcontact imprinting technology combined with the biosensor applications is a promising technology for future applications. © 2014 The Authors.


Erturk G.,Lund University | Erturk G.,Hacettepe University | Hedstrom M.,Lund University | Hedstrom M.,CapSenze HB | And 4 more authors.
Analytica Chimica Acta | Year: 2015

Prostate specific antigen (PSA) is a valuable biomarker for early detection of prostate cancer, the third most common cancer in men. Ultrasensitive detection of PSA is crucial to screen the prostate cancer in an early stage and to detect the recurrence of the disease after treatment. In this report, microcontact-PSA imprinted (PSA-MIP) capacitive biosensor chip was developed for real-time, highly sensitive and selective detection of PSA. PSA-MIP electrodes were prepared in the presence of methacrylic acid (MAA) as the functional monomer and ethylene glycol dimethacrylate (EGDMA) as the cross-linker via UV polymerization. Immobilized Anti-PSA antibodies on electrodes (Anti-PSA) for capacitance measurements were also prepared to compare the detection performances of both methods. The electrodes were characterized by atomic force microscopy (AFM), scanning electron microscopy (SEM) and cyclic voltammetry (CV) and real-time PSA detection was performed with standard PSA solutions in the concentration range of 10fgmL-1-100ngmL-1. The detection limits were found as 8.0×10-5ngmL-1 (16×10-17M) and 6.0×10-4ngmL-1 (12×10-16M) for PSA-MIP and Anti-PSA electrodes, respectively. Selectivity studies were performed against HSA and IgG and selectivity coefficients were calculated. PSA detection was also carried out from diluted human serum samples and finally, reproducibility of the electrodes was tested. The results are promising and show that when the sensitivity of the capacitive system is combined with the selectivity and reproducibility of the microcontact-imprinting procedure, the resulting system might be used successfully for real-time detection of various analytes even in very low concentrations. © 2015 Elsevier B.V.


Liu Y.,Lund University | Chen D.,Lund University | Fan X.,Lund University | Mattiasson B.,Lund University | Mattiasson B.,CapSenze HB
Biosensors and Bioelectronics | Year: 2014

To meet urgent needs for solving serious worldwide drug-resistance problems, a sensitive label-free capacitive sensor developed in our group was investigated as a tool to be applied in the field of antibiotic resistance genotyping, for instance the detection of ampicillin resistance gene (ampR). Proof-of-concept data demonstrated its detection sensitivity of pico-molar without any signal amplification step and a dynamic range of at least three orders of magnitude. The detection limits of less than 1. pM for the single-stranded ampR oligonucleotide and 4. pM for the double-stranded target can reliably be achieved after only 2.5. min sample reaction. Reusability of the probe-functionalized disposable electrode was investigated by comparing different regeneration solutions; mix of 25. mM NaOH/30% formamide was employed to regenerate the electrode for at least six cycles without significant loss of sensing ability. Assay is performed automatically and result is retrieved in 20. min. The developed sensitive genotyping tool is expected to provide simple, fast and affordable screening for monitoring spread of antibiotic resistances, which is suitable for testing in field setting. © 2014 Elsevier B.V.


Lebogang L.,Lund University | Mattiasson B.,Lund University | Mattiasson B.,CapSenze HB | Hedstrom M.,Lund University | Hedstrom M.,CapSenze HB
Microchimica Acta | Year: 2014

We report on the application of an automated and easy-to-use device to directly measure the immunoreactions between adda-specific monoclonal antibodies and microcystins. The antibodies were immobilized on a gold electrode whose surface was modified first with polytyramine and then with gold nanoparticles. The immunoreaction leads to a change in the capacitance of the system. Under optimum conditions, the sensor is capable of performing stable regeneration-assay cycles and has a low detection limit at a concentration of 0.01 pM level of microcystin-leucine-arginine (MC-LR). The surface of the biosensor can be regenerated with pH 2.5 glycine buffer which dissociates the antibody-antigen complex. The biosensor was used to monitor the production of microcystins during batch cultivation of Microcystis aeruginosa (isolated from ponds in Botswana). Liquid chromatography coupled to MS/MS detection was used to identify three variants, viz. MC-LR (995.6 Da), DmMC-LR (981.2 Da) and MC-LA (910.5 Da). [Figure not available: see fulltext.] © 2014 Springer-Verlag Wien.


Erlandsson D.,Capsenze HB | Teeparuksapun K.,Capsenze HB | Teeparuksapun K.,Lund University | Mattiasson B.,Capsenze HB | And 3 more authors.
Sensors and Actuators, B: Chemical | Year: 2014

This document describes a new concept for assessing capacitance based on a constant current pulse to the biosensor transducer. The biosensor has a working electrode that is coated with an insulating molecular layer including a ligand which forms an affinity surface. A sensor electrode is brought into contact with electrolyte solution, and the new measuring principle then involves steps where three different constant currents (I1, I2 and I 3) are serially pulsed on the sensor surface during pre-determined time periods. The potential that is built up (rising) across the sensor surface is sampled every 6.8 μs. The inclination of the registered potential profile corresponding to the current pulsed was utilized to calculate both capacitance and resistance. The new current-based measurement method shows a 10-fold increase in stability for the capacitive measurement as compared to the potential pulse technique. Quantitation of HIV-1 p24 using monoclonal anti-HIV-1 p24 antibodies was used as a model system for the evaluation of the technique. The binding of HIV-1 p24 antigens to the immobilized antibodies causes the capacitance to decrease. The change in capacitance was proportional to the concentration of HIV-1 p24. The capacitance measurement using the current pulse method offers a stable sensing technique with a broad range of potential applications. © 2013 Published by Elsevier B.V.


Lebogang L.,Lund University | Hedstrom M.,Lund University | Mattiasson B.,Lund University | Mattiasson B.,CapSenze HB
Analytica Chimica Acta | Year: 2014

The harmful effects of cyanotoxins in surface waters have led to increasing demands for accurate early warning methods. This study proposes a capacitive immunosensor for broad-spectrum detection of the group of toxic cyclic peptides called microcystins (~80 congeners) at very low concentration levels. The novel analytical platform offers significant advances compared to the existing methods. Monoclonal antibodies (mAbs, clone AD4G2) that recognize a common element of microcystins were used to construct the biosensing layer. Initially, a stable insulating anchor layer for the mAbs was made by electropolymerization of tyramine onto a gold electrode surface, with subsequent incorporation of gold nanoparticles (AuNPs) on the glutaraldehyde (5%) activated polytyramine surface. The biosensor responded linearly to microcystin concentrations from 1×10-13M to 1×10-10M MC-LR standard with a limit of detection of 2.1×10-14M. The stability of the biosensor was evaluated by repeated measurements of the antigen and by determining the capacitance change relative to the original response, which decreased below 90% after the 30th cycle. © 2014 Elsevier B.V.


Patent
Capsenze Hb | Date: 2011-10-31

A method of measuring a capacitance (C) of a sensor having a working electrode that is coated by an insulating layer and a ligand forming an affinity surface. The method involves the steps of bringing the electrode into contact with an analyte, supplying a constant first current (I_(1)), and a constant second current (I_(2)) of opposite direction to the first current (I_(1)), and a constant third current (I_(3)) of same direction as the first current (I_(1)) during determined time periods to the sensor. Further the method includes sampling the potential (V) built up across the sensor, and calculating the capacitance of the sensor from the inclination (B, D, F, H) of a potential curve received in response to the current supply. A method used for detection of an interaction between a ligand and an analyte is also disclosed.

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