CapitalBio Corporation and Tsinghua University | Date: 2017-02-08
In one aspect, the present disclosure provides an integrated microfluidic device for nucleic acid amplification and microarray detection. In one aspect, the device comprises: (1) a microchip configured to process reagents, comprising a plurality of reservoirs, channels, valves, and/or fluid interfaces; (2) an amplification chamber for PCR, carried out in a detachable tube assembled on the microchip through a joint; and (3) a microarray chamber comprising a microarray and a reaction chamber. In some embodiments, these features are interconnected to allow transportation of reagents for nucleic acid amplification and hybridization detection functions in a closed system. In one aspect, the integrate device herein overcomes the problem of contamination during the amplification and hybridization reactions.
CapitalBio eHealth Science & Technology Beijing Co., CapitalBio Corporation, Tsinghua University and Shanghai Sixth Peoples Hospital | Date: 2016-07-06
In one aspect, provided herein are set of primers and use of the same for the detection of SNPs associated with diabetes. In certain embodiments, the primers used to detect SNP sites associated with diabetes comprise Primer Set 1 to Primer Set 47. The experiments show: the genotyping results of the SNP sites associated with diabetes can be accurately detected by the primers disclosed herein, and the risk of individuals can be comprehensively evaluated and the result is more accurate than the single site analysis. In addition, SNPs disclosed herein are verified as associated with type 2 diabetes and its complications, which are especially suitable for the prevention and individualized treatment for type 2 diabetes in East Asian, for example, in China.
Capitalbio Corporation and Tsinghua University | Date: 2015-03-06
A multi-index detection microfluidic chip is provided. A bottom plate (1) and a cover plate (2) that matches the bottom plate (1) and seals it are provided in the microfluidic chip. The center of the microfluidic chip has a through-hole (3). The bottom plate (1) has one or more wave-shaped main channel (s) (4), one end of each of the main channel (4) connected to a sample injection hole (9) on the bottom plate (1), and the other end connected to an exhaust hole (10) on the bottom plate (1). The valley on the main channel (1) is distal to the through-hole (3), and the peak is proximal to the through-hole (3). Each valley of the main channel (1) is connected to a reaction chamber (6) by a linking channel (5). The linking channel (5) comprises one or more buffering chambers (7). The microfluidic chip can be used in detection by fluorescence, turbidity, color, detection equipment, and/or direct observation by the naked eyes. The detection is real-time as the reaction occurs or after the reaction.
CapitalBio Corporation and Tsinghua University | Date: 2014-07-24
In some aspects, the present disclosure provides a magnetic rack comprising a lateral movement structure and/or a longitudinal movement structure. In particular embodiments, the lateral movement structure and/or longitudinal movement structure move one or more magnets to or away from a reaction tube, in order to control the magnetic forces exerted on microbeads in the reaction tube. The microbeads are attracted onto the reaction tube wall, thereby facilitating separation of the microbeads from a solution in the tube. In particular embodiments, the magnetic rack is used to extract or purify nucleic acid from a sample.
CapitalBio Corporation and Tsinghua University | Date: 2014-12-02
A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules.
Capitalbio Corporation and Tsinghua University | Date: 2013-08-23
Provided is a microfluidic chip, which comprises a substrate and a cover sheet, wherein a microreactor array is arranged on the substrate and comprises at least one main channel (401) and at least two micro cells (402) connected to the main channel (401). The microfluidic chip also comprises at least one local temperature control device, which is used for heating the main channel (401) or cooling the micro cells (402). The use of the microfluidic chip ensures uniformity and independency of the micro cells (402). Also provided is an application of the microfluidic chip in biological detection or medical inspection.
CapitalBio Corporation and Tsinghua University | Date: 2016-02-09
The present disclosure in some embodiments provides a flow cell sorting system, a focus detection method, as well as a fluidic chip. In one aspect, the fluidic chip comprises a cover sheet and one or more substrate layers, and one or more channels in each of the fluidic chips. In one aspect, the cells or other analytes in a sample are focused onto the same plane as they flow through the fluidic channel. In another aspect, the cells or other analytes on the same plane are irradiated with the same light intensity, for example, by a flat-top laser beam. This combination eliminates or reduces variation caused by cells at different positions receiving different intensities of the excitation light, and therefore improves accuracy of the flow cytometry analysis. In another aspect, a sorting apparatus is designed at the junction of the detection area and the outlet channel to separate the target cells.
CapitalBio Corporation | Date: 2015-09-25
In some aspects, the present disclosure relates to a method for identifying the type of the unit by camera.
Capitalbio Corporation | Date: 2015-09-25
The present disclosure relates to a method for camera identification and detection on color features. In some aspects, the method comprises: 1) starting the camera, the image processing unit and the display unit at the beginning of testing; 2) using the camera to capture the color characteristic value; and 3) moving the untested object to the detection area of the camera for it to be detected, wherein the image processing unit extracts the mean color characteristic value from the color pixels of the detection area.
Capitalbio Corporation | Date: 2014-02-03
Systems, apparatus and methods for designing and operating a cell-electrode impedance sensor to detect chemical and biological samples, including biological cells. In one implementation, a method of designing a cell-electrode impedance sensor includes determining a cell free cell-electrode impedance and a cell covered cell-electrode impedance based on a design model for the cell-electrode impedance sensor, wherein the design model is based on one or more factors, the factors including properties and elements of a cell-electrode impedance measurement system, using the cell free cell-electrode impedance and the cell covered cell-electrode impedance to obtain a sensor sensitivity of the cell-electrode impedance measurement system, and choosing one or more design parameters of the cell-electrode impedance sensor in the cell-electrode impedance measurement system to maximize the sensor sensitivity.