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Beijing, China

Capital University of Medical science , also known as CUMS, CCMU or CMU, is a university in Beijing, China, which was originally called Beijing Second Medical College .Capital Medical University was founded in 1960, with the original name of Beijing Second Medical College . It ranks among the top academic medical institutions in China and is considered as one of the key municipal universities in Beijing. The founding President, Professor Wu Jieping, was a world-renowned urologist, member of both the Chinese Academy of science and the Chinese Academy of Engineering. The current President is Professor Lu Zhaofeng.CMU consists of 10 Schools, 14 affiliated hospitals and 1 teaching institution. The university and the affiliated hospitals have a staff of about 20,000. Among the staff, there are 6 members of the Chinese Academy of science or the Chinese Academy of Engineering, over 1,000 professors, and over 2,000 associate professors. The university has over 9,000 enrolled students. CMU provides a wide range of educational programs for Doctorates, Masters, Bachelors and certificates.The faculty physicians at CMU train annually several thousands of clinical residents and fellows. Also, the faculty members also provide continuous medical education to physicians and other health professionals through seminars, teaching rounds, and tutorials on and off campus. Over the years, the university has graduated a large number of academic leaders and general practitioners for Beijing and the whole country.CMU is a well known academic institution for its strong competence in the scientific research. It hosts many national and municipal key disciplines, laboratories and some exchange stations for post-doctoral research. High-caliber national and municipal research and training centers and institutes are founded here, covering a wide range of fields including General Practitioner Training, Clinical Medicine, Basic Medicine, Neuroscience, Ophthalmology, Geriatrics, Urology, Cardiology, Pain Medicine, Toxicology, Biomedical Engineering, Traditional Chinese Medicine, Reproduction, Health Policy and so on. We have achieved national and international recognitions in many areas such as Neurobiology, Cytobiology, Immunology, Medical Iconography, Neurology, Neurosurgery, Cardiology, Cardio-vascular Surgery, Kidney Transplantation, Respiratory Disease, Digestive Medicine, Oral-Maxillofacial Surgery, Ophthalmology, Otolaryngology and Pediatric Hematology, among others.CMU provides excellent international exchange programs. It has partnerships and agreements with many universities and institutions with more than 20 countries and regions, supporting activities such as faculty and student exchange programs, joint education and joint research projects.CMU is proud to be at the forefront in the medical education, biomedical research and patient care. It is committed to bring the latest knowledge in basic and clinical science for peoples’ care. Wikipedia.


Wang S.Z.,Capital Medical University
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2012

To investigate the clinical characteristics and responsible agents of drug-induced liver injury (DILI) in pediatric patients. Thirty-one cases of DILI treated in our hospital's pediatric ward were retrospectively analyzed. The clinical data for each patient were extracted from the patient's medical records, and included reported causes, physical and biochemical features, natural history, blood examination results, and hepatic pathology findings. The 31 pediatric cases of DILI accounted for 1.7% of the 1831 total cases of drug-induced liver injury treated at our hospital between February 2002 to June 2011. The pediatric DILI population was composed of 20 males and 11 females, with an average age of 8.8+/-3.9 years old (range, 0.3-14.0). The liver injury patterns represented among the cases were: hepatocellular (25.8%), cholestasis (25.8%), and mixed hepatocellular-cholestatic (48.4%). Antimicrobials were the most common cause (41.9%) of DILI, followed by the herbal medicine (29.0%) and febrifuge drugs (19.4%). A single drug was implicated in nine cases (29.0%), and two or more drugs were implicated in 22 cases (71%). Most of the children had good prognosis, but those with pre-existing disease had poor prognosis. One child died of hepatic failure, making the death rate 3.23%. The average hospitalization time was 25.2 days, and the patients with hepatocellular injury had shorter hospitalization time than those with mixed injury. Drug-induced liver injury in our pediatric population was most often caused by antimicrobials, followed by herbal medicine and febrifuge drugs. Most patients presented with mixed hepatocellular-cholestatic injury. Children with pre-existing diseases or hepatic failure had poor prognosis.


Objective: To explore Akt-regulated direct p53 mitochondrial translocation in cisplatin-induced apoptosis in ovarian cancer cells and the relationship between this and chemoresistance in ovarian cancer. Methods: Chemosensitive ovarian cancer cell lines (OV2008 and A2780s) and chemoresistant cells (C13*and A2780cp) were treated with cisplatin and whole cell and mitochondrial p53 contents were determined by Western blot. The p53 accumulation in mitochondria was determined in purified mitochondrial fractions in cisplatin-sensitive and -resistant ovarian cancer cells. Akt1/2 siRNA were transfected into C13*cells. Cisplatin-induced apoptosis was measured by Hoechst staining and p53 translocation was determined by Western blot. Results: Cisplatin induced mitochondrial p53 accumulation and apoptosis in chemosensitive cells (P < 0.05), but not in resistant cells (P > 0.05). Over-expression of active Akt2 inhibited p53 directly translocate to mitochondria, and downregulation of Akt by Akt1/2 siRNA increased p53 mitochondrial accumulation and sensitize C13*cells to cisplatin treatment. Conclusions: Cisplatin induces direct p53 mitochondrial accumulation in chemosensitive cells, and Akt confers resistance in ovarian cancer cells, in part, by regulating the direct action of p53 in mitochondrial death pathway.


Xu B.,National Center for Cardiovascular Diseases of China | Yang Y.,National Center for Cardiovascular Diseases of China | Yuan Z.,Xian Jiaotong University | Du Z.,New York University | And 3 more authors.
JACC: Cardiovascular Interventions | Year: 2013

Objectives: This study sought to compare clinical outcomes and angiographic findings using the Resolute zotarolimus-eluting stent (R-ZES) (Medtronic, Santa Rosa, California) versus the Taxus Liberte paclitaxel-eluting stent (PES) (Boston Scientific, Natick, Massachusetts) in an all-comer Chinese population. Background: Concerns regarding restenosis risk led to new-generation drug-eluting stents (DES) designed for use in patients with complex clinical or lesion characteristics. In-stent late lumen loss (LLL) is a measure of restenosis risk. Methods: Patients with an indication for treatment with a DES were randomized in a 1:1 ratio to placement of at least 1 R-ZES or PES with minimal exclusions. The primary endpoint was angiographic in-stent LLL at 9 months post-procedure. Clinical endpoints at 12 months are compared between the 2 stents. Results: A total of 198 patients received a R-ZES, and 202 patients received a PES. Most patients were male; 25.8% and 29.2% of R-ZES and PES patients, respectively, had diabetes. Over 70% of lesions in both cohorts were American College of Cardiology/American Heart Association lesion classification Type B2 and C (B2/C). In-stent LLL was 0.16 ± 0.38 mm for R-ZES and 0.33 ± 0.52 mm for PES at 9 months (p < 0.001; 95% confidence interval [CI]: -0.26 to -0.08). The rates of clinically driven target lesion revascularization were 1.5% for R-ZES and 7.0% for PES (p = 0.011). The rate of target lesion failure was 5.6% for R-ZES and 11% for PES (p = 0.068). Conclusions: In an all-comers Chinese population, 9-month in-stent LLL was significantly less with R-ZES compared with PES, which was reflected in lower revascularization rates at 12 months for the R-ZES patients. Results are consistent with previous clinical trials of the R-ZES in all-comer populations. (Resolute Zotarolimus-Eluting Stent Versus the Taxus Liberte Paclitaxel-Eluting Stent for Percutaneous Coronary Intervention in China [R-China RCT]; NCT01334268). © 2013 American College of Cardiology Foundation.


Fang S.,Capital Medical University
TheScientificWorldJournal | Year: 2013

Dengue is an old disease caused by the mosquito-borne dengue viruses (DENVs), which have four antigenically distinct serotypes (DENV1-4). Infection by any of them can cause dengue fever (DF) and/or a more serious disease, that is, dengue hemorrhagic fever (DHF) or dengue shock syndrome (DSS). In recent decades, incidence of dengue disease has increased 30-fold, putting a third to half of the world's population living in dengue-endemic areas at high infection risk. However, the pathogenesis of the disease is still poorly understood. The virus binding with its host cell is not only a first and critical step in their replication cycle but also a key factor for the pathogenicity. In recent years, there have been significant advances in understanding interactions of DENVs with their target cells such as dendritic cells (DC), macrophages, endothelial cells, and hepatocytes. Although DENVs reportedly attach to a variety of receptors on these cells, consensus DENV receptors have not been defined. In this review, we summarize receptors for DENVs on different cells identified in recent years.


Chai S.B.,Capital Medical University
Sheng li ke xue jin zhan [Progress in physiology] | Year: 2013

Leptin is a product of the obese (ob) gene and acts through its receptor Ob-R. Leptin is primarily known for its role as a hypothalamic modulator of food, especially in intake, energy balance, fat stores and body weight. Recent studies have shown that leptin may be involved in the development of respiratory diseases such as pulmonary artery hypertension, chronic obstructive pulmonary disease, lung neoplasms and asthma. Therefore, further studies are needed to elucidate the mechanisms accounting for the association between leptin and respiratory diseases, which may lead to the development of novel approaches to the prevention and treatment of these diseases. Here we give an overview of the distribution and physiological function of leptin and ob-R, and summarize the recent progress in the relationship between leptin and respiratory diseases.


Gu Z.,State University of New York at Buffalo | Cheng J.,State University of New York at Buffalo | Zhong P.,State University of New York at Buffalo | Qin L.,State University of New York at Buffalo | And 3 more authors.
Journal of Neuroscience | Year: 2014

Degeneration of basal forebrain (BF) cholinergic neurons is one of the early pathological events in Alzheimer’s disease (AD) and is thought to be responsible for the cholinergic and cognitive deficits in AD. The functions of this group of neurons are highly influenced by glutamatergic inputs from neocortex. We found that activation of metabotropic glutamate receptor 7 (mGluR7) decreased NMDARmediated currents and NR1 surface expression in rodent BF neurons via a mechanism involving cofilin-regulated actin dynamics. In BF cholinergic neurons, β-amyloid (Aβ) selectively impaired mGluR7 regulation of NMDARs by increasing p21-activated kinase activity and decreasing cofilin-mediated actin depolymerization through a p75 NTR-dependent mechanism. Cell viability assays showed that activation of mGluR7 protected BF neurons from NMDA-induced excitotoxicity, which was selectively impaired by Aβ in BF cholinergic neurons. It provides a potential basis for the Aβ-induced disruption of calcium homeostasis that might contribute to the selective degeneration of BF cholinergic neurons in the early stage of AD. © 2014 the authors.


Background: Circular excision by secondary-intention healing is useful for small skin defects (diameter <10 mm). But the injection pain as the local anesthesia is administered one of the patients’ biggest concerns. Buffered lidocaine can effectively relieve the injection pain, but it might increase the potential complications. This study examined the effect of buffered lidocaine on wound healing. Methods: Patients undergoing circular excision by secondary-intention healing were divided into 2 groups and were administered buffered or unbuffered lidocaine as the local anesthesia. Comparative parameters included injection pain, postoperative pain, complication rate, scar width, and scar quality at 6 months. Statistical differences between the 2 agents were assessed. Results: The patients in the buffered group experienced 0.9 fewer scaled injection pain units than did the patients in the unbuffered group (p = 0.009). Complication rates and scar appearance did not show any statistical difference between the 2 groups. Conclusion: Buffered lidocaine is useful to relieve the injection pain during the secondary-intention healing, and it is safe to use if it is applied properly. © 2015 Informa UK, Ltd.


Jiang C.,Capital Medical University
Journal of neurointerventional surgery | Year: 2013

Direct surgical exposure and cannulation (DSEC) of the superior ophthalmic vein (SOV) can be used as an alternative approach for the endovascular treatment of cavernous sinus dural fistulas (CSDF) that fail conventional endovascular access. The aim of this study was to report the techniques, effectiveness and safety of DSEC of the SOV in these cases. Between June 2007 and June 2011, nine CSDF in nine patients who presented with ocular signs and symptoms were embolized using DSEC of the SOV when the CSDF could not be treated via the transarterial or transfemoral venous route. All nine patients were successfully treated by introduction of Onyx in conjunction with detachable coils through the catheterized SOV to the affected cavernous sinus. No exposure or catheterization of the SOV proved difficult. Symptoms and signs resolved completely in all patients after successful occlusion of the CSDF. There were no intraoperative complications. All patients underwent follow-up cerebral angiography at least 3 months after treatment, and none showed recurrence of the fistula. DSEC of the SOV for embolization of a CSDF is a good alternative treatment when conventional venous routes are inaccessible. Surgical access to the SOV is direct and can be performed safely. The use of Onyx in conjunction with detachable coils is safe and effective for symptomatic CSDF.


BACKGROUND AND PURPOSE—: Minor stroke and transient ischemic attack are common disorders with high rate of subsequent disabling stroke. We aim to investigate the role of high-sensitive C-reactive protein (hsCRP) in predicting recurrent stroke and poor functional outcome. METHODS—: In the Clopidogrel in High-Risk Patients With Acute Nondisabling Cerebrovascular Events (CHANCE) trial, 3044 (59%) consecutive patients from 73 (64%) prespecified centers had hsCRP levels measured. The primary outcome was any stroke within 90 days. The secondary outcome included combined vascular events and dependence or death defined as modified Rankin Scale score of 2 to 6 at 90 days and a new vascular event during 1-year follow-up. The associations of hsCRP with recurrent stroke and functional outcome were analyzed by using Cox proportional hazards and logistic regression models. RESULTS—: Elevated hsCRP (>3.0 mg/L) was observed in 32% of the study population. Patients with hsCRP >3 mg/L had an increased risk of recurrent stroke (adjusted hazard ratio, 1.46; 95% confidence interval, 1.08–1.98; P=0.039), ischemic stroke and combined vascular events, and poor functional outcome (adjusted odds ratio, 1.68; 95% confidence interval, 1.22–2.32; P=0.002) compared with those with hsCRP <1 mg/L within 90-day follow-up period. High hsCRP levels also independently predicted recurrent stroke during 1-year follow-up. There was no interaction of hsCRP levels with randomized antiplatelet therapy. CONCLUSIONS—: High hsCRP levels predict recurrent stroke and poor functional outcome in acute patients with minor stroke or transient ischemic attack. CLINICAL TRIAL REGISTRATION—: URL: http://www.clinicaltrials.gov. Unique identifier: NCT00979589. © 2016 American Heart Association, Inc.


Tan D.,Raffles Cancer Center | Chng W.J.,National University of Singapore | Chou T.,Niigata Cancer Center Hospital | Nawarawong W.,Chiang Mai Medical School | And 5 more authors.
The Lancet Oncology | Year: 2013

Treatment of multiple myeloma has undergone substantial developments in the past 10 years. The introduction of novel drugs has changed the treatment of the disease and substantially improved survival outcomes. Clinical practice guidelines based on evidence have been developed to provide recommendations on standard treatment approaches. However, the guidelines do not take into account resource limitations encountered by developing countries. The huge disparities in economy, health-care infrastructure, and access to novel drugs in Asian countries hinder the delivery of optimum care to every patient with multiple myeloma in Asia. In this Review we outline the guidelines that correspond with different levels of health-care resources and expertise, with the aim to unify diagnostic and therapeutic guidelines and help with the design of future studies in Asia. © 2013 Elsevier Ltd.


Shen L.,Capital Medical University
Retina | Year: 2016

PURPOSE:: To assess differences in scleral and choroidal thickness between eyes with secondary high axial myopia caused by congenital glaucoma, eyes with primary high axial myopia, and nonhighly myopic eyes. METHODS:: The study consisted of 301 Chinese individuals with a mean age of 23.9 ± 22.6 years and mean axial length of 24.8 ± 4.2 mm. It included the “secondary highly myopic group” (SHMG) because of congenital glaucoma (n = 20 eyes; axial length >26.0 mm), the “primary highly myopic group” (PHMG) (n = 73; axial length >26.0 mm), and the remaining nonhighly myopic group (NHMG). RESULTS:: The secondary highly myopic group versus the primary highly myopic group had significantly thinner sclera in the pars plana region (343 ± 71 μm versus 398 ± 83 μm; P = 0.006), whereas scleral thickness in other regions did not differ significantly between both highly myopic groups and was significantly thinner in both highly myopic groups than in the NHMG. Mean total scleral volume did not differ significantly (P > 0.20) between any group (SHMG: 659 ± 106 μm; PHMG: 667 ± 128 μm; NHMG: 626 ± 135 μm). Choroidal thickness was significantly thinner in both highly myopic groups than in the NHMG, with no significant differences between both highly myopic groups. Choroidal volume did not differ significantly (P > 0.40) between any of the groups (SHMG: 43 ± 12 μm; PHMG: 43 ± 13 μm; NHMG: 46 ± 17 μm). CONCLUSION:: In secondary high axial myopia, the sclera gets thinner anterior and posterior to the equator; whereas in primary high axial myopia, scleral thinning is predominantly found posterior to the equator. Because volume of sclera and choroid did not differ between any group, scleral and choroidal thinning in myopia may be due to a rearrangement of tissue and not due to the new formation of tissue. © 2016 by Ophthalmic Communications Society, Inc.


Chan S.Y.,Capital Medical University
Retina | Year: 2016

PURPOSE:: Using optical coherence tomographic angiography (OCT-angiography), we examined the vasculature of the choriocapillaris in patients with central serous chorioretinopathy (CSC). METHODS:: The prospective observational clinical study included patients with CSC defined by painless loss of central visual acuity and presence of a serous macular detachment as visualized by spectral-domain OCT. All eyes underwent OCT-angiography. Subfoveal choroidal thickness and choriocapillaris width were measured. RESULTS:: The study included 26 eyes of 21 patients with a mean age of 47.0 ± 7.9 years (range: 34–65 years). All 26 eyes showed an image pattern of high signal intensity, and 21 eyes additionally demonstrated dilated capillaries in the OCT-angio images. The areas showing abnormalities in the OCT-angiography were congruent with leaking areas in fluorescein angiography or areas with hyperpermeability in indocyanine green angiography. Among 16 patients with unilateral CSC, 1 patient showed a high intensity pattern in the OCT-angiogram in the contralateral, clinically unaffected eye. Parallel to thicker choroidal thickness measurements in affected eyes as compared with contralateral unaffected eyes in unilateral CSC, OCT-angiography revealed significantly thicker choriocapillaris measurements in the affected eyes. Conventional fluorescein angiography did not demonstrate leakage in 14 of 18 eyes with clinically diagnosed CSC. CONCLUSION:: Optical coherence tomographic angiography showed an image pattern of high signal intensity in all eyes with CSC and dilated vessels in the choriocapillaris in most eyes with clinically diagnosed CSC. The results indicate that OCT-angiography may become a noninvasive valuable tool for the diagnosis of CSC in particular and for the diagnosis of macular disorders in general. © 2016 by Ophthalmic Communications Society, Inc.


BACKGROUND/PURPOSE:: To examine the diagnostic value of optical coherence tomography (OCT) for the detection of acute Vogt–Koyanagi–Harada (VKH) disease. METHODS:: Clinical charts and OCT images were retrospectively reviewed for patients consecutively diagnosed with acute VKH, subacute VKH, multifocal central serous chorioretinopathy (CSCR), and posterior scleritis. All patients underwent OCT, fundus photography, and fluorescein angiography (FA) before treatment. The characteristics of OCT and FA were analyzed and recorded. RESULTS:: The study included 80 eyes with acute VKH, 32 eyes with subacute VKH, 33 eyes with CSCR, and 13 eyes with posterior scleritis. The most common OCT features of VKH disease were hyperreflective dots (70/80; 88%), subretinal membranous structures (64/80; 80%), retinal detachment higher than 450 μm (63/80; 79%), and retinal pigment epithelium (RPE) folds (44/80; 55%). For the detection of VKH disease, sensitivity and specificity were for subretinal membranous structures 80% and 95.6%, respectively, for high retinal detachment 78.8% and 76.1%, respectively, for subretinal hyperreflective dots, 87.5 and 60.9%, respectively, and for RPE folds 55% and 80.4% respectively. Subretinal membranous structures showed the highest positive predictive value (97.3%) and negative predictive value (65.7%) of all OCT assessed features. CONCLUSION:: OCT-related morphological signs have a relatively high predictive value for the diagnosis of acute VKH. © 2016 by Ophthalmic Communications Society, Inc.


Wu J.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2012

To evaluate the adrenocortical function in children with severe and critical enterovirus 71 infection by using a high-dose (250 μg) adrenocorticotropic hormone (ACTH) stimulation test. And to at provide experimental basis for glucocorticoid in the treatment of hand-foot-and-mouth disease (HFMD). This was a prospective multi-center study which was carried out in PICUs of Beijing Children's Hospital, Zhengzhou Children's Hospital, Kaifeng Children's Hospital and Linyi People's Hospital in Shandong province. Children with severe and critical hand-foot-mouth disease admitted to PICUs of the four hospitals from June 2009 to April 2010 were enrolled in this study, and EV71 virus nucleic acid test and high-dose (250 μg) ACTH stimulation started at the same time. EV71 virus nucleic acid positive 51 cases were eventually enrolled in the study. Cortisol test was performed at baseline (T0) and after high-dose (250 μg) ACTH stimulation at 30 minutes (T30), 60 minutes (T60) in the first 6 hours after admission, but before glucocorticoid was given. The adrenocortical function was evaluated according to ΔTmax [ΔTmax=(T30, T60 maximum)-T0]. Diagnostic criteria of adrenal insufficiency (AI) is increment (ΔTmax)≤9 μg/dl. The incidence of AI in 51 cases was 52.94% (27/51). The incidence of AI in severe group was 44.74% (17/38), which was significantly higher in critical group 76.92% (10/13), P<0.05. Of the cases with a pediatric critical illness score (PCIS)≤70, 81.82% (9/11) had adrenal insufficiency, and it was 28.57% (4/14) when PCIS≥90. The incidence of AI was 75% (6/8) and 48.84% (21/43) in death and survivor group respectively, but there were no significant difference between the two groups (P>0.05). Baseline (T0) cortisol in death group was higher than survivor group (P<0.05). AI may occur in children with enterovirus 71 infection. The critical enterovirus 71 infection had a high incidence of AI. AI may affect the prognosis of patients with severe and critical enterovirus 71 infection. Exogenous glucocorticoids administration may be considered when AI is identified or highly suspected. The timing, dosage and regimen of glucocorticoid are still unclear. Further animal experiments and clinical trials are needed.


Lou Y.Q.,Capital Medical University
Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] | Year: 2011

To evaluate the relationship between A46G and C79G polymorphisms in the β2-adrenergic receptor (ADRB2) gene and the incidence of essential hypertension (EH) among the Han Chinese population. We conducted a computer retrieval of PUBMED, EMBASE, CNKI, Wanfang and VIP databases prior to May 2010. Articles investigating the relationship of EH and ADRB2 gene polymorphism of Han group were found through literature search, including 15 articles on A46G and 10 articles on C79G. According to the including and excluding criteria, a Meta-analysis was conducted in EH and ADRB2 gene polymorphism of A46G and C79G. The association was examined by RevMan4.2 software through quantitative analysis. Eight articles on A46G polymorphism (including 1078 EH cases and 788 control subjects) and six articles on C79G polymorphism (including 1367 EH cases and 1006 control subjects) were included in the current study. Meta-analysis showed that there was a significant association between A46G polymorphism and EH: genotype GG/(AA + AG) (fixed-effected model, OR = 1.35, 95%CI = 1.04 - 1.74, P = 0.02), genotype GG/AA (fixed-effected model, OR = 1.41, 95%CI = 1.06 - 1.89, P = 0.02). No significant association was found between C79G polymorphism and EH of Han group in China: G/C allele comparison (random-effected model, OR = 0.88, 95%CI = 0.55 - 1.39, P = 0.57). Significant association was found between A46G polymorphism of ADRB2 gene and EH, whereas no association could be found between C79G polymorphism and EH among Han Chinese population.


To investigate the key microanatomic and radiological structures of optic canal comprehensively so as to provide anatomic parameters and procedural flows for the decompression of optic canal. Gross observations and microscopic measurements were applied on 10 (20 sides) formalin-treated cadaveric specimens and 15 (30 sides) adult skulls. Using multislice helical CT (computed tomography)-aided three-dimensional reconstruction in combination with direct anatomic measurement, the investigators dissected, photographed, measured and analyzed the shape of optic canal and analyze its anatomic relationship with the adjoining structures. Optic canal was formed by the superior, inferior, medial and external walls and distal proximal opening. The lateral wall of optic canal was formed by anterior clinoid process with a length of (9.87 ± 1.34) mm, a width of (11.66 ± 2.35) mm, a base thickness of (5.35 ± 1.07) mm and a middle thickness of (4.50 ± 1.06) mm. Optic strut separating the optic canal from the superior orbital fissure was located inferiorly. And the distance between the apex of anterior clinoid process and the middle of ICA (internal carotid artery) groove was (4.25 ± 2.30) mm. The CSF (cerebrospinal fluid) leakage and secondary injury of optic nerve and injury of ICA, ophthalmic artery might occur during the surgical procedures due to the variation of anterior clinoid process. The microanatomic figures and radiological measurements had a mean difference very close to each other at (0.08 - 0.48) mm. No statistical difference was found (P > 0.05). Optic nerve, ophthalmic artery and ICA may be exposed by a high-speed drilling of the lateral wall of optic canal. The drilling dissection of lateral wall plays a vital role during a successful optic canal decompression. Radiological measurement and three-dimensional reconstruction of skull base may be of great clinical significance in lesion visualization. And it helps to make a better choice of surgical approaches. The measurements provide valuable references for surgeons and researchers.


Zhang C.,Capital Medical University
Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology | Year: 2011

To observe the clinical effect of iatrogenic perforation repaired by mineral trioxide aggregate (MTA). Twenty-one iatrogenic perforation cases were included. The root canals were shaped and cleaned and then obturated after MTA was used to repair the perforation with microscopes. Preoperative, immediate postoperative, and follow-up radiographs were evaluated to determine the pathologic changes adjacent to the perforation site. Nineteen cases healed and two cases were healing. No cases failed. MTA is effective in the treatment of iatrogenic perforation.


Yang Y.,Capital Medical University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2012

To evaluate the feasibility of screening for pediatric obstructive sleep apnea hypopnea syndrome (OSAHS) according to OSA-18,physical examination and electronic nasopharyngoscopy. Outpatients with snoring received questionnaire, physical examination and electronic nasopharyngoscopy in Pediatric Sleep Center of Beijing Children's Hospital from 2009.1 to 2009.12. All children were divided into OSAHS or non-OSAHS group based on the results of polysomnography (PSG). The material was compared between these two groups. The differences of age,tonsil scores, adenoid scores,total OSA-18 sores, the loudness of snoring scores, sleep asthma or suffocation scores, worrying lack of oxygen scores were significant (P < 0.05). And then put them into the logistic equation Y and make ROC analysis, if Y is higher than 0. 735, these children were more likely with OSAHS. The sensitivity was 62.7% and the specificity was 79.4%. It is feasible to screen for pediatric OSAHS according to questionnaire, physical examination and electronic nasopharyngoscopy.


Zhang H.,Chinese Academy of Sciences | Yuan X.,Chinese Academy of Sciences | Chen S.,Capital Medical University | Zhao Y.,Chinese Academy of Sciences | And 6 more authors.
Circulation | Year: 2015

Background-Conflicting results from recent observational studies have raised questions concerning the benefit of β-blockers for patients undergoing coronary artery bypass grafting (CABG). Furthermore, the efficacy of long-term β-blocker therapy in CABG patients after hospital discharge is uncertain. Methods and Results-The study included 5926 consecutive patients who underwent CABG and were discharged alive. The prevalence and consistency of β-blocker use were determined in patients with and without a history of myocardial infarction (MI). β-Blockers were always used in 1280 patients (50.9%) with and 1642 patients (48.1%) without previous MI after CABG. Compared with always users (n=2922, 49.3%), the risk of all-cause death was significantly higher among inconsistent β-blocker users (hazard ratio [HR], 1.96; 95% confidence interval [CI], 1.50-2.57), and never using β-blockers was associated with increased risk of both all-cause death (HR, 1.42; 95% CI, 1.01-2.00) and the composite of adverse cardiovascular events (HR, 1.29; 95% CI, 1.10-1.50). In the cohort without MI, the HR for all-cause death was 1.70 (95% CI, 1.17-2.48) in inconsistent users and 1.23 (95% CI, 0.76-1.99) in never users. In the MI cohort, mortality was higher for inconsistent users (HR, 2.14; 95% CI, 1.43-3.20) and for never users (HR, 1.59; 95% CI, 1.07-2.63). Consistent results were obtained in equivalent sensitivity analyses. Conclusions-In patients with or without previous MI undergoing CABG, the consistent use of β-blockers was associated with a lower risk of long-term mortality and adverse cardiovascular events. Strategies should be developed to understand and improve discharge prescription of β-blockers and long-term patient adherence. ©2015 The Authors.


Li X.,Capital Medical University
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2013

The objective of this study was to analyse the changes of inflammatory cytokines level in traumatic rat serum after maggot secretions intervention. Acute traumatic rats were randomly divided into three groups that included maggot secretions group, negative group, and the control group. TNF-α, IL-6, SOD, LPO levels were measured by radioimmunoassay. The experimental results showed that TNF-α, IL-6, and SOD levels in the model group were significantly increased; LPO level was decreased and showed significant differences. Thus, the content of inflammatory cytokines in acute skin wounds could be reduced by maggot secretions, which play a role in enhancing wound healing.


To compare the detection rates of prostate cancer (PCa) and complications of the transrectal ultrasonography (TRUS)-guided 24-core saturation scheme versus 14-core scheme for transperineal prostate biopsy in patients with total PSA < 20 microg/L. We performed TRUS-guided 24-core saturation transperineal biopsy for 136 patients suspected of PCa (24-core group) and 14-core biopsy for another 116 (14-core group). We compared the PCa detection rates and post-biopsy complications, such as gross hematuria, urinary system infection, and acute urinary retention between the two groups. The baseline characteristics of the two groups were comparable with regard to the mean age, prostate volume and PSA level (P>0.05). The positive rates of PCa detection were 48.53% (66/136) in the 24-core group and 17.24% (20/116) in the 14-core group (P<0.001), and the positive rates of samples were 8.09% and 2.83%, respectively (P=0.012). The detection rate of PCa in the apex zone was significantly higher in the former (11.76%) than in the latter (1.72%) (P<0.05). There were no statistically significant differences in such post-biopsy complications as gross hematuria, urinary system infection, and acute urinary retention between the two groups (P>0.05). TRUS-guided 24-core saturation transperineal biopsy of the prostate is superior to the 14-core scheme for its higher detection rate of PCa, particularly PCa in the apex zone, and lower incidence of complications in patients with PSA < 20 microg/L.


Yang Y.,Capital Medical University
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2011

To investigate the effect of 5-FU (5-fluorouracil) on enriching cancer stem cells of HCC cell line BEL-7402 and the biological characteristics of enriched cells. The enriching concentration of 5-FU was determined by CCK-8 (cell counting kit-8). Flow Cytometry was used to determine the changes in cell cycle and positive expression ratio of surface marker CD56, CD54, EpCAM and CD133. The self-renewal and differentiation of positive cells were tested by colony formation assay, and were compared with the control group. Enriching concentration of 5-FU was determined as 10 μg/ml with 48 h incubation. After enrichment, G0/G1 phase cells increased from 57.50 %+/-0.98% to 68.70%+/-3.41% (P<0.05). Whereas S phase cells decreased from 40.26%+/-4.12% to 31.80%+/-4.15% (P<0.01); G2/M phase cells disappeared in experimental group, and was 5.80%+/-1.87% in control group (P<0.01). The proportion of the cell cycle changed with significant statistical differences. Meanwhile, positive rate of cell surface makers CD56, CD54, EpCAM and CD133 increased from 0.57%+/-0.12%, 8.10%+/-6.79%, 0.3%+/-0.01% and 3.20%+/-0.99% to 4.13%+/-0.06%, 50.08%+/-1.69%, 0.55%+/-0.07% and 10.51%+/-1.13%, respectively. The difference was significant (P<0.05). The colony forming ratio of CD56, CD54, EpCAM and CD133 negative cells and positive cells were 2.11%+/-0.21%, 3.32%+/-0.31%; 0.86%+/-0.101%, 2.40%+/-0.52 %; 7.19%+/-0.56%, 7.73%+/-0.71%; 2.70%+/-0.26%, 5.75%+/-0.81%, respectively, and significant differences were found between (P<0.05). 5-fluorouracil enriched the cancer stem cell population in HCC cell line BEL-7402. CD56 and CD54 can be used as important surface markers in research of liver cancer stem cells.


Zhou N.,Ohio University | Zhou N.,University of Michigan | Huang J.,Johns Hopkins University | Chen X.,Capital Medical University | Xu L.,Ohio University
Otology and Neurotology | Year: 2013

HYPOTHESIS: Performance in tone perception and production are correlated in prelingually deafened pediatric cochlear implant (CI) users across individuals. Demographic variables, such as age at implantation, contribute to the performance variability. BACKGROUND: Poor representation of pitch information in CI devices hinders pitch perception and affects perception of lexical tones in cochlear implant users who speak tonal languages. METHODS: One hundred ten Mandarin-speaking, prelingually deafened CI subjects and 125 typically developing, normal-hearing subjects were recruited from Beijing, China. Lexical tone perception was measured using a computerized tone contrast test. Tone production was judged by native Mandarin-speaking adult listeners as well as analyzed acoustically and with an artificial neural network. A general linear model analysis was performed to determine factors that accounted for performance variability. RESULTS: CI subjects scored ∼67% correct on the lexical tone perception task. The degree of differentiation of tones produced by the CI group was significantly lower than the control group as revealed by acoustic analysis. Tone production performance assessed by the neural network was highly correlated with that evaluated by human listeners. There was a moderate correlation between the overall tone perception and production performance across CI subjects. Duration of implant use and age at implantation jointly explained ∼29% of the variance in the tone perception performance. Age at implantation was the only significant predictor for tone production performance in the CI subjects. CONCLUSION: Tone production performance in pediatric CI users is dependent on accurate perception. Early implantation predicts a better outcome in lexical tone perception and production. © 2013, Otology & Neurotology, Inc.


Wang L.H.,Capital Medical University
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2013

To investigate the effect of local application of paclitaxel on airway scar formation after airway injury in rabbits. Forty New Zealand rabbits were randomly divided into four groups, negative control group (n = 10), saline control group (n = 10), group I (n = 10), group II (n = 10). All rabbits received tracheotomy. In negative control group, the specimens were harvested for histological examination and immunohistochemical analysis immediately after tracheotomy;in other three groups, rabbits received airway injury after tracheotomy. In group I and group II, 0.4 mg/ml and 1.0 mg/ml paclitaxel was applied locally in injured airway segment for 3 minutes after airway injury. The normal saline was used in control group for 3 minutes. The animals were killed in 21 days after operation. The specimens were harvested for histological examination and immunohistochemical analysis. Transmission electron microscopy was employed to observe the ultrastructure of paclitaxel-induced apoptotic cells. The degree of stenosis in group I and group II were significantly decreased compared to those in saline control group [saline control group (59 ± 13)%, group I (27 ± 8)%, group II (22 ± 7)%]. Histological examination showed fibroblast cells and inflammatory cells in group I and group II were significantly fewer than in saline control group. The TGF-β1 positive cells and VEGF positive cells in group I and group II were significantly decreased compared to those in saline control group (P < 0.05). Paclitaxel-induced cell apoptosis and injured cell organs were observed by transmission electron microscopy. Local application of paclitaxel inhibits airway scar formation after airway injury in rabbit model, and the inhibition is dose dependent. Paclitaxel may have a therapeutic potential for the treatment of airway stenosis caused by endotracheal intubation, tracheotomy or implantation of airway stents.


Chen L.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2012

To explore the clinical and laboratory features and the prognosis of juvenile dermatomyositis (JDM) complicated with interstitial lung disease (ILD). Data of 39 cases of JDM complicated with ILD hospitalized in Beijing Children's Hospital from January 2005 to December 2011 were collected. The clinical features, laboratory data and prognosis of these children were analyzed. Of the 39 cases studied, 16 were boys, and 23 girls. The average age of onset was 5.6 years, and 61.5% of the patients' age of onset (24 cases) was under 6 years. Rashes (17 cases, 43.6%), simultaneous eruption of rashes and muscle weakness (14 cases, 35.9%), fever (4 cases, 10.1%), or muscle weakness (3 cases, 7.7%) were common initial symptoms of the disease. Only 51.3% of the patients (20 cases) had the symptoms of respiratory system, but (24 cases) 61.5% were complicated with that of the gastrointestinal system; (27 cases) 69.2% had at the same time electrocardiographic and echocardiographic abnormalities. The chest high resolution computed tomography (HRCT) showed cord or band-like shadows in their lungs of more than half of the cases (25 cases, 64.1%), and other changes included ground glass-like shadow (10 cases, 25.6%), net and lineation-like shadow (9 cases, 23.1%), nodular change (5 cases, 12.8%). The patients complicated with lung essential infiltration accounted for as high as 71.8% (28 cases). These imaging changes were largely seen on both dorsal sides of their lungs. Severe patients also had mediastinal emphysema, pneumothorax, pneumorrhagia or aerodermectasia. Twenty-four patients underwent pulmonary function examination, and 62.5% of the patients' pulmonary function (15 cases) was abnormal. The fatality rate of the cases studied was 10.1%. The imaging changes of patients suffering from JDM with ILD were often more severe as compared to the clinical symptoms, and were often complicated with damages to other systems and organs. The prognosis of those patients was poorer than others. Patients with JDM especially at a younger age of onset and with various organ damages should be examined with chest HRCT examinations as early as possible.


Wang J.,Capital Medical University
African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines | Year: 2013

The objective of this paper was to study the extraction methods of tannin constituents from Semen Cuscutae and their anti-papilloma effects. Single factor test and orthogonal design methods were used to determine the optimal extraction method; the mouse skin papilloma model induced by DMBA/croton oil was established, which was a classic two-stage carcinogenesis model being used to observe and evaluate the anti-carcinogenic effects of tannins extracted from Semen Cuscutae in different stages. The optimal extraction method of Semen Cuscutae was a 20-fold volume of solvent, a temperature of 50 °C, three times of extraction, with 20 min each, skin papilloma experiment revealed that the number of bearing tumors gradually reduced, and the inhibition rate gradually increased with the increase of dose, in the high-dose group, its inhibition rate reached 70.2%. Tannin extract from Semen Cuscutae has an obvious inhibitory effect on skin papilloma development.


Xu J.,Capital Medical University
Cellular and molecular neurobiology | Year: 2012

Some anesthetics have been suggested to induce Alzheimer's disease (AD) neuro-pathogenesis. Increasing evidence indicates that hyperphosphorylated tau plays a key role in the pathogenic events that occur in AD. Isoflurane has been shown to induce apoptosis, which leads to accumulation of amyloid-β (Aβ). We set out to investigate whether isoflurane can induce apoptosis by increasing hyperphosphorylated tau in Aβ25-35-induced cells and the underlying mechanism. Cultured rat pheochromocytoma cells (PC12) were exposed to 20 mM Aβ25-35 alone or with 2% isoflurane for 6 h. The cell viability was determined by MTT assay, and the apoptosis rate was detected by flowcytometry. Western blotting and immunocytochemical staining were performed to observe the protein expression of Bcl-2 family, tau phosphorylation of different sites, tau protein kinases and phosphatases. Additionally, lithium chloride was administered to all above groups to investigate the changes of apoptosis rate and protein expression. The apoptosis rate was significantly increased in Aβ25-35 group compared with the others groups, which was accompanied by bcl-2 decline, and the phosphorylation of glycogen synthase kinase-3β (GSK-3β) and tau of two sites increased. LiCl attenuated the cellular apoptosis by inhibition the level of tau phosphorylation. Isoflurane upregulated the level of phosphorylated GSK-3β, which phosphorylate tau at different sites, and aggravated the apoptotic rate of the Aβ25-35-induced PC12 cells. It indicated that isoflurane-induced tau phosphorylation might play a role in the AD-like development.


Asymmetric dimethylarginine (ADMA), an endogenous nitric oxide synthase inhibitor, is considered to be an independent risk factor in the progression of chronic kidney diseases (CKD). It can induce kidney fibrosis by increasing transforming growth factor (TGF)-β1 expression, but its molecular mechanism is unclear. The aim of the present study was to investigate the role of actin cytoskeleton in ADMA-induced TGF-β1 high expression in human renal glomerular endothelial cells (HRGECs). The structure of stress fibers was visualized by immunofluorescence, nuclear factor-κB (NF-κB) DNA-binding activity was assessed by an electrophoretic mobility shift assay and TGF-β1 expression was assessed by western blot analysis. Results showed that ADMA induced the assembly of stress fibers, DNA binding of NF-κB, and increasing expression of TGF-β1. When the dynamics of actin cytoskeleton was perturbed by the actin-depolymerizing agent cytochalasin D and the actin-stabilizing agent jasplakinolide, or ablation of stress fiber bundles by the nicotineamide adenine dinucleotide phosphate oxidase inhibitor apocynin and p38 mitogen-activated protein kinase inhibitor SB203580, ADMA-induced DNA binding of NF-κB and TGF-β1 expression were inhibited. These results revealed an actin cytoskeleton-dependent mechanism in ADMA-induced NF-κB activation and TGF-β1 high expression in HRGECs. The specific targeting of the actin cytoskeleton may be a useful strategy to prevent ADMA-activated kidney fibrosis in CKD.


OBJECTIVE: This study sought to explore the impact of metabolic syndrome (MS) on the risk of recurrence after catheter ablation of long-standing persistent atrial fibrillation (AF).METHODS: Totally 248 patients [197 male, (56±12)years] with persistent AF and catheter ablation were included. Long-standing persistent AF was defined based on the duration (more than one year).RESULTS: Among the 248 patients, 96 (38.7%) patients had MS, 130 (52.4%) patients had long-standing persistent AF. After 91-1222 (404±303) days follow-up, 119 (47.9%) had recurrence. The recurrence rate was significantly higher in the MS group than that in the non-MS group (58.3% vs 41.4%, P = 0.017). The proportion of MS was similar between the long-standing persistent AF group and the non-long-standing persistent AF group (36.9% vs 40.7%, P = 0.544). Subjects with MS had higher recurrence rate than those without MS in non-long-standing AF group (56.3% vs 32.9%, P = 0.033), but not in long-standing AF group (60.4% vs 48.8%, P = 0.177). In multivariate analysis, MS (hazard ratio 1.98, 95% CI 1.04-3.76, P = 0.036) and AF history duration (hazard ratio 1.04, 95% CI 1.01-1.07, P = 0.004) were independent risk factors for recurrence after catheter ablation of AF. Long-standing persistent AF was not an independent risk factor of recurrence.CONCLUSION: MS was natively associated with the success rate of catheter ablation of AF in patients with non-long-standing persistent AF, but not in patients with long-standing persistent AF.


Zhang J.,Capital Medical University
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2012

To explorer the clinical features, diagnosis and therapy of Langerhans cell sarcoma (LCS). The clinical data of a case of LCS originated from cervical lymph nodes was analyzed. The pathological biopsy was studied by cell morphology, immunohistochemistry and electron microscopy, and the related literature was reviewed. The giant tumor cells were characterized by markedly malignant proliferation, irregular nuclei and obviously chromatin abnormality, the positive S-100, CD1a and Langerin (CD207) tumor cells were revealed by immunohistochemistry, and Birbeck granules could be found by electron microscopy. All of them supported the diagnosis of LCS. The patient's condition progressed rapidly and died of multiple organ failure in a short time. LCS is an extremely rare neoplastic proliferation of Langerhans cells with overtly malignant cytologic features and spreads aggressively. The diagnosis of LCS mainly relies on pathological cell morphology, immunohistochemistry and electron microscopy if necessary. The treatment includes chemotherapy, surgery and radiotherapy, etc, but lack of generally accepted optimal treatment regimen currently. In short, LCS has intensive invasiveness and poor prognosis.


Shen Y.M.,Capital Medical University
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2011

To evaluate the safety and efficacy of hernioplasty using acellular tissue matrix patch to repair inguinal hernia of pediatric patients aged 6 to 18 years. Sixty eligible patients aged 6 to 18 years with primary unilateral inguinal hernia were randomly assigned to experimental or control group from June to December 2009. In the experimental group, acellular tissue matrix patch was used during Lichtenstein herniorrhaphy while traditional high ligation of hernial sac was used in the control group. Preoperative and postoperative parameters such as clinical informations of patients, postoperative complications and recurrence rate were recorded and analyzed. There were no significant differences between the 2 groups in postoperative length of stay [(31 ± 8) h vs. (34 ± 11) h] and postoperative Visual Analogue Scale Pain Score (2.8 ± 0.9 vs. 2.6 ± 1.0) (P > 0.05), but the operation time in the experimental group were longer than that in the control group significantly [(39 ± 4) min vs. (36 ± 4) min, t = 3.357, P = 0.001]. The duration of follow-up ranged from 14 to 20 months. There were no postoperative incisional infection, chronic postoperative pain and local foreign body sensation in two groups. In the experimental group, 3 patients suffered scrotal hydrocele as compared 2 patients in the control group. There was no recurrence in the experimental group as compared 2 patients (6.7%) in the control group, which was no significant difference (P > 0.05). Lichtenstein repair for pediatric patients aged 6 to 18 years with acellular tissue matrix patch has good results and with limited postoperative complications.


Shi B.Y.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To investigate the difference of transient evoked otoacoustic emissions (TEOAE) between neonates and adults with the frequency-specific approach. TEOAE were recorded from 112 newborns (62 females, 58 males) who passed hearing screening, and 32 adults (17 females, 15 males) tested with pure-tone threshold equal or less than 20 dBHL. Band reproducibility, signal-to-noise rate (SNR) of frequency-band and half-octave frequency analysis of TEOAE amplitude was recorded respectively. Significant differences were found in two groups, the higher entire TEOAE level found in newborn [(15.18 +/- 4.39) dB SPL] was higher than that of adults group [(9.51 +/- 4.12) dB SPL, P < 0.05]. The lowest wave reproducibility and SNR were in first frequency band (0.8 kHz) for neonate, and then, in last frequency band (4.0 kHz) were for young adults. For newborns, the highest band reproducibility and SNR were presented at fourth frequency band (3.2 kHz). However, for adults, the highest band reproducibility and SNR were presented at second frequency band (1.5 kHz). Frequency space of the SNR response peaks between neonates and adults was 1.7 kHz. The level difference was 7.09 dB SPL. Half octave frequency analysis shows a TEOAE response peaks in neonates was (10.50 +/- 5.09) dB SPL at the 2828 Hz, and in adults, it was (2.84 +/- 5.33) dB SPL at the 1414 Hz. Frequency space of the TEOAE response peaks between neonates and adults was 1414 Hz. Level differences of TEOAE response peaks was 7.66 dB SPL. TEOAE response peak in adult appear at 1.5 kHz, and then dropped down correlated to increase of frequency. From the first to the last testing frequency band, the difference of TEOAE level between neonate and adult was found to increase correlated to increase of frequency. TEOAE response level in newborn is higher than that in adult group. Further, the decrease of TEOAE response level presented more sharpens with the frequency increasing in adult. The frequency of TEOAE response peak in neonate (3.2 kHz) is higher than adult (1.5 kHz). The level of TEOAE response peak is also greater than adult.


Zhang X.T.,Capital Medical University
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2012

To evaluate the association between triglyceride (TG) level and newly identified 5-year carotid plaque and to explore the prediction value of TG level on the newly-identified carotid plaque with risk factors of traditional atherosclerosis. A cohort study was adopted. The baseline survey including CVD risk factors and B-mode ultrasound of carotid artery was performed in 2002, and the second follow-up examination was performed in 2007. We evaluated 1949 participants with lipid measurements and B-mode ultrasound of carotid arteries in the two surveys (with mean age as 57.9±8.1 years and 39.2% were men). The baseline TG levels were divided into four groups: group 1 (TG<1.13 mmol/L), group 2 (TG=1.13-1.69 mmol/L), group 3 (TG=1.70-2.25 mmol/L) and group 4 (TG≥2.26 mmol/L). Newly identified carotid plaque was regarded as the indicator of progression of carotid atherosclerosis. New relationship between fasting TG levels and newly identified carotid plaque was analysed. Compared to newly identified carotid plaque which including different TG level groups, the incidence of newly artery plaque had significantly increased along with the increase of baseline triglyceride level (30.8%, 38.8%, 41.9% and 44.2% respectively, with χ2=21.22, P<0.01). Compared to individuals (TG<1.13 mmol/L), TG seemed a risk factor of plaque progression (P<0.01). After adjusted for age, sex, dyslipidemia and other risk factors, high TG group (TG≥2.26 mmol/L) appeared a significant independent predictor of newly identified carotid plaque (OR=1.37, 95%CI: 1.00-1.86). When further stratifying the traditional atherosclerosis risk factors, we found that high TG group with smoking or hypertension was an independent factor of atherosclerosis progression. With the increase of triglyceride levels, the rate of newly identified carotid plaque also increased. After adjusting age, sex, dyslipidemia and other risk factors, serum fasting TG≥2.26 mmol/L appeared to be an independent predictor of newly developed carotid plaque.


Wang C.S.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2011

To evaluate the efficacy and immunological changes of children receiving subcutaneous immunotherapy with Dermatophagoides pteronyssinus. Sixty-four children with allergic rhinitis to Dermatophagoides pteronyssinus (Der p) were randomly allocated to receive either specific immunotherapy (n = 32) or medical treatment (n = 32). Symptom and medication scores were assessed to evaluate the clinical efficacy in the baseline and after one year treatment. Total IgE, Der p-specific IgE, and specific IgG4 were measured. Immunotherapy reduced the symptom (the scores reduced from 10[9;11] to 4[3;6]) and medication score (the scores reduced from 0.76[0.61;0.90] to 0.35[0.30;0.43]) in children with allergic rhinitis significantly(Z value were -4.80 and -4.74, respectively, each P < 0.01). There was a significant difference in symptom and medication scores between both groups after one year treatment (U value were 155.00 and 139.50, respectively, each P < 0.01). There were no differences in levels of serum total IgE, specific IgE before and after one year treatment, but the level of serum specific IgG4 increased significantly after one year treatment. Immunotherapy with standardized extract is efficacious to treat children sensitive to Der p, allergen-specific IgG4 is significant as immunological marker to predict efficacy of immunotherapy.


Jung B.,University Hospital Freiburg | Stalder A.F.,Capital Medical University | Bauer S.,University Hospital Freiburg | Markl M.,University Hospital Freiburg
Magnetic Resonance in Medicine | Year: 2011

The purpose of this study was to explore how to optimally undersample and reconstruct time-resolved 3D data using a k-t-space-based GRAPPA technique. The performance of different reconstruction strategies was evaluated using data sets with different ratios of phase (N y) and partition (N z) encoding lines (N y × N z = 64-128 × 40-64) acquired in a moving phantom. Image reconstruction was performed for different kernel configurations and different reduction factors (R = 5, 6, 8, and 10) and was evaluated using regional error quantification and SNR analysis. To analyze the temporal fidelity of the different kernel configurations in vivo, time-resolved 3D phase contrast data were acquired in the thoracic aorta of two healthy volunteers. Results demonstrated that kernel configurations with a small kernel extension yielded superior results especially for more asymmetric data matrices as typically used in clinical applications. The application of k-t-GRAPPA to in vivo data demonstrated the feasibility of undersampling of time-resolved 3D phase contrast data set with a nominal reduction factors of up to R net = 8, while maintaining the temporal fidelity of the measured velocity field. Extended GRAPPA-based parallel imaging with optimized multidimensional reconstruction kernels has the potential to substantially accelerate data acquisitions in time-resolved 3D MRI. © 2011 Wiley-Liss, Inc.


Recent studies implicate the regulatory function of microRNAs (miRNAs) in oocyte maturation and ovarian follicular development. Differentially expressed miRNAs are found in the plasma of premature ovarian failure (POF) patients and normal cycling women. In this study, miRNA-regulated signaling pathways and related genes were described using Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis. The effect of mir-23a on granulosa cell apoptosis was also studied by examining the protein expression of X-linked inhibitor of apoptosis protein (XIAP) and caspase-3, followed by subsequent counting of apoptotic cells after Hoechst 33258 staining. Both GO analysis and pathway analysis suggested that many signaling pathways, including the AKT signaling pathway, steroid hormone receptor signaling pathways, and others, were regulated by this group of differentially expressed miRNAs. A decrease in XIAP expression (mRNA and protein level) and caspase-3 protein levels and an increase in cleaved caspase-3 protein were observed in human ovarian granulosa cells transfected with pre-mir-23a, along with an increased occurrence of apoptosis. In conclusion, differentially expressed miRNAs in the plasma of POF patients may have regulatory effects on proliferation and apoptosis of granulosa cells by affecting different signaling pathways. Mir-23a may play important roles in regulating apoptosis via decreasing XIAP expression in human ovarian granulosa cells.


Zhang Q.-E.,Capital Medical University
Journal of ECT | Year: 2016

PURPOSE: Little is known about the use of electroconvulsive therapy (ECT) for adolescent psychiatric patients in China. This study examined the frequency of ECT and the demographic and clinical correlates of adolescent psychiatric patients hospitalized in a tertiary psychiatric hospital in China. METHODS: This was a retrospective chart review of 954 inpatients aged between 13 and 17 years treated over a period of 8 years (2007–2013). Sociodemographic and clinical data were collected from the electronic chart management system for discharged patients. RESULTS: The rate of ECT use was 42.6% in the whole sample (46.5% for patients with schizophrenia, 41.8% for major depressive disorder, 57.8% for bipolar disorders, and 23.9% for other diagnoses). Use of ECT was independently and positively associated with older age, high aggression risk at time of admission, and use of antipsychotics and antidepressants. Compared with patients with schizophrenia, those with other psychiatric diagnoses were less likely to receive ECT. The above significant correlates explained 32% of the variance of ECT use (P < 0.001). Limitations of this study included the lack of data regarding the efficacy and side effects of ECT. Furthermore, the high rate of ECT applied only to 1 setting which limits the ability to extrapolate the implications of the results to other populations. CONCLUSIONS: The use of ECT was exceedingly high in adolescent patients treated in a tertiary clinical centre in China. It is unlikely that such a high rate of ECT use is found across China or that such practice reflects standard of care for psychiatrically ill adolescents. The underlying reasons for the high use of ECT at this center warrant urgent investigations. Copyright © 2016 Wolters Kluwer Health, Inc. All rights reserved


Chen M.,Capital Medical University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011

To study the clinical features and conservative treatment of petrositis. One case of petrositis was reported and literatures were reviewed. Opacificated air cells in CT, and anomalous, discontinuous hypersignal in MRI T1 and T2 provided evidence of infection of the apex of the petrous temporal bone. The patient recovered totally after conservative treatment with intravenous antibiotic, glucocorticoid and neurotrophic therapy, and no recurrence occurred in 1 year follow-up. CT rescan showed the clear air cells of the petrous apex 4 months later. Petrositis can be diagnosed with specific clinical features, the image of CT and MRI. Conservative treatment could be a good choice.


Zhao Y.,Capital Medical University
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011

To compare the effect of proximal needling and routine acupuncture for sciatica. Sixty patients with sciatica were randomly divided into a proximal needling group and a routine acupuncture group, 30 cases in each group. The proximal needling group was treated by proximal needling at Huantiao (GB 30). The routine acupuncture group was treated by acupuncture at Huantiao (GB 30), Yinmen (BL 37), Weizhong (BL 40), Yanglingquan (GB 34) etc. with routine acupuncture method. The Numerical Pain Rating Scales (NPRS) the Japanese Orthopaedic Association Scores (JOA) were all observed before, after treatment and in following up. The total effective rates were 100.0% (30/30) in both groups, with no significant difference between the two groups (P > 0.05) in 3 month follow up. After 5 treatments, the total improvement rate of 100.0% (30/30) in the proximal needling group was super to that of 80.0% (24/30) in the routine acupuncture group. The NPRS and JOA scores were all improved significantly after 5 treatment and in following up compared with those before treatment in two groups (all P < 0.01). After treatments, the improvement of the NPRS and JOA scores in the proximal needling group was greater than those in the routine acupuncture group (both P < 0.05). The proximal needling treatment has rapid and obvious therapeutic effect and analgesia on sciatica.


Chang Y.-J.,Hebei Medical University | Weng C.-L.,Xiamen University | Sun L.-X.,Hebei Medical University | Zhao Y.-T.,Capital Medical University
Annals of Hematology | Year: 2012

Controversy remains regarding the transplant outcomes of human leukocyte antigen-identical related bone marrow transplantation (BMT) and peripheral blood stem cell transplantation (PBSCT) for the treatment of patients with hematological malignancies. To provide an estimate of the effect of BMT and PBSCT on clinical outcomes in patients with hematological malignancies, we conducted a meta-analysis based on time-to-event data from 17 randomized controlled trials. PubMed, EMBASE, and Cochrane Central Register of Controlled Trials (CENTRAL), from 1972 through July 2010, and conference proceedings through July 2009 and reference lists, without any language restriction, of randomized trials that compared the transplant outcomes after BMT and PBSCT in patients with hematological malignancies were searched for details. Two independent reviewers extracted the data. The outcomes examined were engraftment, graft-versus-host disease (GVHD), relapse, transplant-related mortality (TRM), leukemia-free-survival (LFS), and overall survival (OS). Compared to PBSCT, BMT had lower neutrophil (HR, 2.08; 95% CI, 1.80 to 2.42; p < 0.00001) and platelet (HR, 2.77; 95% CI, 1.78 to 4.30; p < 0.00001) engraftment. BMT was associated with a significant decrease in the development of grades II-IV (HR, 0.75; 95% CI, 0.63 to 0.90; p = 0.002) and III-IV (HR, 0.63; 95% CI, 0.47 to 0.84; p = 0.001) acute GVHD as well as overall (HR, 0.70; 95% CI, 0.59 to 0.83; p < 0.0001) and extensive (HR, 0.60; 95% CI, 0.39 to 0.91; p = 0.002) chronic GVHD. BMT was associated with a higher incidence of relapse (HR, 1.91; 95% CI, 1.34 to 2.74; p = 0.0004). Comparable TRM (1.08; 95% CI, 0.56 to 2.10; p = 0.81), LFS (HR, 1.04; 95% CI, 0.83 to 1.30; p = 0.73), and OS (HR, 1.06; 95% CI, 0.81 to 1.39; p = 0.65) were demonstrated for both treatments. An inverse linear relationship was observed between the acute GVHD difference (PBSCT minus BMT) and the outcome of OS (p = 0.016). Our meta-analysis suggest that BMT leads to slower hematological recovery, increasing rates of relapse, and a lower risk of GVHD, but no significant difference in LFS and OS. A lower incidence of acute GVHD is associated with a superior OS. © 2011 Springer-Verlag.


Mao J.,Center for Atrial Fibrillation | Yin X.,Center for Atrial Fibrillation | Zhang Y.,Capital Medical University | Yan Q.,Center for Atrial Fibrillation | And 3 more authors.
Circulation: Arrhythmia and Electrophysiology | Year: 2014

Background-Previous studies have suggested that systematic ablation of ganglionated plexi (GP) could increase the shortterm success rate of radiofrequency ablation for atrial fibrillation, but the long-term efficacy of this approach is not fully established. Methods and Results-Twenty-four mongrel dogs were divided into 3 groups: epicardial GP ablation group 1 (n=8), epicardial GP ablation group 2 (n=8), and a sham operation group (n=8). In the 2 epicardial GP ablation groups, the 4 major GP and the ligament of Marshall were systematically ablated. The effective refractory period and inducibility of tachyarrhythmias were measured before and immediately after GP ablation in epicardial GP ablation group 1 and 8 weeks later in the other 2 groups. Tyrosine hydroxylase and choline acetyltransferase expressions were also determined immunohistochemically 8 weeks later in the latter groups. Compared with epicardial GP ablation group 1 and the sham operation group, epicardial GP ablation group 2 had the shortest atrial and ventricular effective refractory period and the highest inducibility of atrial tachyarrhythmias. The inducibility of ventricular tachyarrhythmias among the 3 groups was comparable. The density of tyrosine hydroxylase- and choline acetyltransferase-positive nerves in the atrium was the highest in epicardial GP group 2, whereas there were no significant intergroup differences in the densities of these 2 types of nerves in the ventricle. Conclusions-After 8 weeks of healing, epicardial GP ablation without additional atrial ablation was potentially proarrhythmic, which may be attributable to decreased atrial effective refractory period and hyper-reinnervation involving both sympathetic and parasympathetic nerves. © 2014 American Heart Association, Inc.


Objectives: To find out whether there is a difference in the incidence of injection pain and other complications using pre-cooling versus the buffered equivalent in upper blepharoplasty. Methods: A prospective, randomized study in patients scheduled for primary upper blepharoplasty was performed. Each subject was his/her own control by performing pre-cooling for 2 min before plain lidocaine injection in one eyelid, while the buffered solution injection was used in the other eyelid. Data were collected regarding injection pain, postoperative pain, bleeding, bruising, swelling and scar appearance. Results: Sixty patients participated in this study. Injection pain, checked immediately, revealed a mean operative pain rating of 2.20 ± 0.32 in the eye with pre-cooling versus 2.30 ± 0.35 in the buffered lidocaine (p = 0.074). A statistical difference was observed in postoperative pain after 2–4 h, with the pre-cooling group having a score of 4.00 ± 0.14 versus 4.40 ± 0.30 in the buffered lidocaine group (p = 0.021). The postoperative pain after 24 h was 2.00 ± 0.56 in the pre-cooling group versus 2.30 ± 0.23 in the buffered lidocaine group (p = 0.006). There were no statistical differences between the buffered and unbuffered lidocaine eyes after 2 days or 1 week in regard to postoperative pain, bleeding, swelling, bruising and scar appearance. Conclusions: Pre-cooling could induce similar injection pain relief to that of buffered lidocaine while maintaining longer postoperative anesthetic results than buffered lidocaine. © 2015 Taylor & Francis.


Zhang X.-J.,Capital Medical University
Ophthalmology in China | Year: 2011

Optic atrophy is clinically common and usually accompanied with different severity of visual function impairment. The key point is that the clinical manifestation and visual outcomes are different among the patients with optic atrophies caused by different conditions. Special attention should be paid to distinguishing optic atrophy and congenital optic disc anomaly, isolated inherited optic neuropathy and optic atrophy as part of the systemic neurological diseases, and some conditions mimicking optic atrophy. Precise understanding of optic atrophy is very important for finding the causes, starting good treatment strategy and eventually protecting the patients' visual function.


He R.X.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2012

Most of Endobronchial tuberculosis (EBTB) of children is caused by the bronchial erosion from the scrofula close to the bronchus. Due to its complicated mechanism, pediatricians have limited knowledge on the endobronchial tuberculosis, which makes the misdiagnosis rate high. This work explored the clinical features and diagnostic methods of endobronchial tuberculosis (EBTB), to improve the understanding of this disease. A total of 102 cases with endobronchial tuberculosis (EBTB) were investigated by analyzing clinical and bronchoscopic features, imaging manifestation, PPD results and etiological examination; 71 cases were male, 33 were female, the youngest was 4 months old, and the oldest was 10 years old. The patients were treated in the fourth ward of the Department of Internal Medicine, Beijing Children's Hospital, Capital Medical University. Of the 102 cases, 71 (69.6 percent) were infants and young children. (1) Age and clinical presentation: Infants and young children were more common, the main clinical manifestations were fever and cough, physical examination may find decreased breath sounds, wet and dry rale and wheezing. (2) In 81 cases who received CT there was bronchial obstruction, in 43 cases there were simultaneous manifestations of primary pulmonary tuberculosis (mediastinal and hilar lymph node enlargement and/or pulmonary consolidation), 21 cases had only manifestations of primary tuberculo. (3) The results of PPD: the positive rate was 95%. (4) Bronchoscopic feature: presence of mucosal swelling, caseous necrosis, bronchial stenosis/extrabronchial compression, granulomatous lesions and lymph node bronchial fistula. (5) Bacteriologic results: 21 patients were bacteriologically positive. Bronchial tuberculosis was more common in infants and young children, their major clinical manifestations were fever, cough, common findings on lung imaging was mediastinal and hilar lymph node enlargement and/or pulmonary consolidation, bronchial obstruction should be considered manifestations of bronchial tuberculosis, positive PPD should support the clinical diagnosis. Diagnosis can be confirmed by positive bacteriological tests.


Ma X.L.,Capital Medical University
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2012

This study was purposed to analyze the relation of N-myc gene copy number with clinical staging, pathological types and tumor biological factors in children with neuroblastoma (NB), and to investigate the influence of chemotherapy on N-myc gene expression and explore the relationship of N-myc gene copies with prognosis of NB children. The newly diagnosed children with NB from 1 March 2007 to 31 January 2011 were enrolled in this study. The treatment was carried out by BCH-NB-2007 based on Hongkong NB-07 protocol, and the patients were follow up to 31 January 2012. The N-myc gene in NB children was detected by FISH. According to number of N-myc gene copies, the NB children were divided into 3 groups. A group (N-myc gene negative) had less than 2 copies, B group (N-myc gene gains) had 3 to 9 copies, and C group (N-myc amplification) had more than 10 copies. The results showed that the N-myc gene expression in 58 cases of NB was observed. There were 36 males and 22 females. NB children aged from 6.5 to 138 months (median age 47.5 months), all patients were followed up for 11 - 57 months with an average of 31.5 months. INSS stages I-IV were 1, 5, 8 and 44 cases, respectively. Twenty-five cases had primary post mediastinal tumor, thirty-three cases had retroperitoneal and pelvic tumor, three of which also companied with post mediastinal tumor. Thirty-five cases had bone metastasis (60.3%), thirty-two cases had bone marrow metastasis (55%). Of the 54 patients with fully known biologic features, seventeen cases had ganglioneuroblastoma, thirty-seven cases had neuroblastoma (15 displayed differentiated, 7 poorly differentiated or undifferentiated, 15 with pathological changes after chemotherapy), four cases had bone marrow metastasis only detected by bone marrow biopsy. Eleven cases had N-myc gene negative, forty-three had N-myc gains, four had N-myc amplification. The average copy number of N-myc gene copies in 58 cases was 5.96 ± 7.81 in which 28 children were non chemotherapy cases, their average copy number was 4.00 ± 1.88, thirty cases out of 58 cases received preoperation chemotherapy (chemotherapy group), and their average copy number was 7.80 ± 10.46, the difference is significant (P = 0.064). The clinic stage, the location of primary tumor, pathological classification, urine VMA and serum neurogenic specific enolase had no effects on the N-myc gene expression, but the serum LDH level had influence (P < 0.01). Single factor Kaplan-Meier analysis showed that the number of N-myc gene copies in NB patients were closely related with the poor prognosis. The more copies of N-myc gene, the more poor prognosis, the difference is statistically significant (P < 0.05). It is concluded that the number of N-myc gene copies correlates with the rapid growth of NB and its poor prognosis, detecting the N-myc amplification can help to estimate the prognosis and decide the program of treatment. Serum LDH, which correlated with the rapid growth of NB, had effect on the N-myc gene expression and is closely related with the poor prognosis of NB.


Zhang X.-H.,Capital Medical University
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2012

Multiple sclerosis (MS) is an inflammatory demyelinating disease in central nervous system. The etiology is still unknown. The pathogenesis may be related to autoimmune response. Clinical features are dissemination in time (multiple attacks) and dissemination in space (multifocal episode). The common used examinations including brain or spinal MRI, CSF analysis and evoked potentials. The latest diagnostic criteria is McDonald criteria published in 2010. It is now accepted that neuromyelitis optica (NMO) is different from MS in clinical, pathology, imaging and so on. Due to the diversity in clinical manifestation and the lack of specific biological parameters, the diagnosis of multiple sclerosis is still challenging.


Yuan L.,Capital Medical University
Clinical Nuclear Medicine | Year: 2015

ABSTRACT: A 1-year 5-month-old male patient had a history of recurrent cough and pneumonia, which required antibiotics and bronchodilators. CT suggested the possibility of congenital tracheobiliary fistula. However, a diagnosis could not be made based on CT alone. A Tc-EHIDA hepatobiliary scintigraphy was then performed, which led to a definite diagnosis of tracheobiliary fistula. © 2015 Wolters Kluwer Health, Inc. All rights reserved.


Liu J.R.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2012

To investigate clinical characteristics and predictive factors of refractory Mycoplasma pneumoniae pneumonia (RMPP) in children so as to recognize and treat the disease earlier. The data including febrile time, inflammatory markers (WBC, neutrophil, CRP) and radiological features of 213 children hospitalized with Mycoplasma pneumoniae pneumonia (MPP) (72 with refractory MPP and 141 with mild MPP were retrospectively analyzed). The primary diagnostic criteria of refractory MPP: the patient's condition still deteriorates after treatment with macrolides for more than 5 days. The independent variables which had significant difference in univariate analysis was analyzed by multivariate logistic regression analysis. The predictive criteria of RMPP were further applied in 100 other patients prospectively. Kappa test was used to evaluate the accuracy rate. Refractory MPP patients: febrile time was more than 10 days, white blood cell (WBC) count was (3.8 - 18.5)×10(9)/L in peripheral blood routine test, CRP was 38 mg/L - > 160 mg/L, large lobar consolidation with high density (> 2/3 pulmonary lobe, CT value 40 - 50 HU, without air bronchogram). Mild MPP patients: febrile time was less than 10 days, CRP was often less than 40 mg/L. Independent risk factors for RMPP were febrile time, CRP, large consolidation area with high density in lungs with or without pleural effusion (OR = 1.586, P = 0.017; OR = 4.344, P = 0.001; OR = 2.660, P = 0.012), CT value 40 - 50 HU which were demonstrated by logistic regression analysis. The specificity, sensitivity and Youden index for this diagnostic test were respectively 0.96, 0.94 and 0.90 at a CRP cut off of 40 mg/L. The sensitivity, specificity, and Kappa value for the above criteria to diagnose RMPP were respectively 0.96, 0.94 and 0.9. The predictive factors for RMPP are febrile time (> 10 days), CRP (> 40 mg/L), large lobar consolidation with high density (> 2/3 pulmonary lobe, CT value > 40 HU with or without pleural effusion) for the purpose of treating earlier.


Li C.-S.,Southern Illinois University Carbondale | Li C.-S.,Jiamusi University | Chung S.,Korea Institute of Science and Technology | Lu D.-P.,Capital Medical University | Cho Y.K.,Gangneung - Wonju National University
Journal of Neurophysiology | Year: 2012

The parabrachial nuclei (PbN), the second central relay for the gustatory pathway, transfers taste information to various forebrain gustatory nuclei and to the gustatory cortex. The nucleus accumbens is one of the critical neural substrates of the reward system, and the nucleus accumbens shell region (NAcSh) is associated with feeding behavior. Taste-evoked neuronal responses of PbN neurons are modulated by descending projections from the gustatory nuclei in the forebrain. In the present study, we investigated whether taste-responsive neurons in the PbN project to the NAcSh and whether pontine gustatory neurons are subject to modulatory influence from the NAcSh in urethaneanesthetized hamsters. Extracellular single-unit activity was recorded in the PbN, and taste responses were confirmed by the delivery of 32 mM sucrose, NaCl, quinine hydrochloride, and 3.2 mM citric acid to the anterior tongue. The NAcSh was then stimulated (0.5 ms, ≤100 μ A) bilaterally using concentric bipolar stimulating electrodes. A total of 98 taste neurons were recorded from the PbN. Eighteen neurons were antidromically invaded from the NAcSh, mostly the ipsilateral NAcSh (n =16). Stimulation of the ipsilateral and contralateral NAcSh suppressed the neuronal activity of 88 and 55 neurons, respectively; 52 cells were affected bilaterally. In a subset of pontine neurons tested, electrical stimulation of the NAcSh during taste stimulation also suppressed taste-evoked neuronal firing. These results demonstrated that taste-responsive neurons in the PbN not only projectto the NAcSh but also are under substantial descending inhibitory influence from the bilateral NAcSh. © 2012 the American Physiological Society.


Wang N.,Capital Medical University
Chinese Journal of Ophthalmology | Year: 2015

In the last 5 years, great achievements have been made in glaucoma clinics and research field in China. The main Chinese glaucoma research progresses were selected by Chinese Ophthalmology Society. Among them, some research works have reached the international level. But we should clearly realize that Chinese glaucoma research work has a long way to go and the development plan should be made carefully. In the future, we need to establish the national clinical standards of glaucoma diagnosis and treatment to improve the research level, so as to enhance our international influence in glaucoma field. Copyright © 2015 by the Chinese Medical Association.


Wei Q.,Capital Medical University
Zhongguo shi yan xue ye xue za zhi / Zhongguo bing li sheng li xue hui = Journal of experimental hematology / Chinese Association of Pathophysiology | Year: 2011

Epstein-Barr virus (EBV) is the major triggering factor producing virus-associated hemophagocytic syndrome (VAHS). The clinical manifestations were various and it's diagnosis should meet HLH-2004 criteria as well as EBV-positive, and familial hemophagocytic lymphohistiocytosis and lymphoma-associated hemophagocytic syndrome must be ruled out, too. In terms of treatment, combination of dexamethasone, VP-16 and cyclosporin A is the first choice, if they do not work, hematopoietic stem cell transplantation can be done. In this review, the recent advance on pathogenesis, clinical features, diagnostic problems, treatment strategies and prognosis of EBV-HLH have been described.


Sonneveld M.J.,Erasmus Medical Center | Hansen B.E.,Erasmus Medical Center | Piratvisuth T.,Prince of Songkla University | Jia J.-D.,Capital Medical University | And 8 more authors.
Hepatology | Year: 2013

On-treatment levels of hepatitis B surface antigen (HBsAg) may predict response to peginterferon (PEG-IFN) therapy in chronic hepatitis B (CHB), but previously proposed prediction rules have shown limited external validity. We analyzed 803 HBeAg-positive patients treated with PEG-IFN in three global studies with available HBsAg measurements. A stopping-rule based on absence of a decline from baseline was compared to a prediction-rule that uses HBsAg levels of <1,500 IU/mL and >20,000 IU/mL to identify patients with high and low probabilities of response. Patients with an HBsAg level <1,500 IU/mL at week 12 achieved response (HBeAg loss with HBV DNA <2,000 IU/mL at 6 months posttreatment) in 45%. At week 12, patients without a decline in HBsAg achieved a response in 14%, compared to only 6% of patients with HBsAg >20,000 IU/mL, but performance varied across HBV genotype. In patients treated with PEG-IFN monotherapy (n=465), response rates were low in patients with genotypes A or D if there was no decline of HBsAg by week 12 (negative predictive value [NPV]: 97%-100%), and in patients with genotypes B or C if HBsAg at week 12 was >20,000 IU/mL (NPV: 92%-98%). At week 24, nearly all patients with HBsAg >20,000 IU/mL failed to achieve a response, irrespective of HBV genotype (NPV for response and HBsAg loss 99% and 100%). Conclusion: HBsAg is a strong predictor of response to PEG-IFN in HBeAg-positive CHB. HBV genotype-specific stopping-rules may be considered at week 12, but treatment discontinuation is indicated in all patients with HBsAg >20,000 IU/mL at week 24, irrespective of HBV genotype. © 2013 by the American Association for the Study of Liver Diseases.


To build a three-dimensional finite element model of thoracolumbar spine with osteoporotic vertebral compression fracture (OVCF) and analyze its biomechanical change. The T10-L2 segment data were obtained from computed tomography (CT) scans of an elderly female with a single T12 OVCF. A three-dimensional finite element model of thoracolumbar spine was constructed with the MIMICS and ABAQUS software. The model was composed of bony vertebrae, articulating facets, intervertebral disc and associated ligaments. The basic stress analysis of T10-L2 motion segment was made for different material properties of bone, ligaments and facet joints contacting frictional property. The stress on the annulus fiber, nucleus pulposus, endplate and facet joints under axial pressure (0.3 MPa, 1.0 MPa, 4.0 MPa) were analyzed. A three-dimensional finite element model of human T12-L2 motion segment had 617468 elements. And the stress was higher in vertebral body than posterior structure. The distribution of pressure stresses in intervertebral disc was asymmetrical. The stress increased with a rising axial pressure. 3D finite element model of thoracolumbar OVCF and adjacent segments are successfully established. The results of stress analysis are both feasible and reliable.


Ruan X.,Capital Medical University | Seeger H.,University Hospital of Tuebingen | Mueck A.O.,University Hospital of Tuebingen
Maturitas | Year: 2012

Nomegestrol acetate (NOMAC) is a 19-norprogesterone derivative with high biological activity at the progesterone receptor, a weak anti-androgenic effect, but with no binding to estrogen, glucocorticoid or mineralocorticoid receptors. At dosages of 1.5 mg/day or more, NOMAC effectively suppresses gonadotropic activity and ovulation in women of reproductive age. Hemostasis, lipids and carbohydrate metabolism remain largely unchanged. In normal and cancerous human breast cells, NOMAC has shown favorable effects on estrogen metabolism. Like natural progesterone (but in contrast to some other synthetic progestogens), it does not appear stimulate the proliferation of cancerous breast cells. While there has been some experience of the use of NOMAC in combination with estrogens as a hormone replacement therapy, most of the data on the compound are reported in the context of its inclusion as a component of a new contraceptive pill comprising 2.5 mg NOMAC combined with 1.5 mg estradiol. Because of its strong endometrial efficacy, and due to its high antigonadotropic activity and long elimination half-life (about 50 h), the contraceptive efficacy of the new pill is maintained even when dosages are missed. Furthermore, for the first time with a monophasic 24/4 regimen containing estradiol, cyclical stability can be achieved comparable with that obtained using pills containing ethinyl estradiol and progestogens like levonorgestrel or drospirenone. The addition of NOMAC to estradiol means that the beneficial effects of estrogen are not lost, which is of especial importance in relation to the cardiovascular system. On the basis both of its pharmacology and of studies performed during the development of the NOMAC/estradiol pill, involving some 4000 women in total, good long-term tolerability can be expected for NOMAC, although its safety profile is still to be fully ascertained, as the clinical endpoint studies are yet to be completed. © 2012 Elsevier Ireland Ltd. All rights reserved.


Ruan X.,Capital Medical University | Seeger H.,University Hospital of Tuebingen | Mueck A.O.,University Hospital of Tuebingen
Maturitas | Year: 2012

Dienogest (DNG) is a 19-nortestosterone derivative (a C-19 progestogen) with a cyanomethyl instead of an ethinyl group at the C-17 position, which may make the compound elicit fewer hepatic effects than other C-19 nortestosterone derivatives. Its similarity to norethisterone is reflected in its high endometrial efficacy, which could explain the high stability of the menstrual cycle women achieve when they use DNG in combination with ethinyl estradiol (EE) or with estradiol valerate (E2V). Its strong endometrial efficacy underlies the use of DNG (on its own) to treat endometriosis, and gives it antiproliferative and anti-inflammatory effects in the treatment of endometriotic lesions. Properties derived from its C-19 derivative structure include its short plasma half-life, of about 10 h (which means the drug is not accumulated), and its high oral bioavailability, of more than 90%. However, DNG also has some of the properties of typical of progesterone derivatives, including a lack of effect on the metabolic and cardiovascular systems, and considerable antiandrogenic activity, the latter increased by its lack of affinity to the sex-hormone binding globulin (SHBG), in contrast to other C-19 progestogens. DNG has no glucocorticoid and no antimineralocorticoid activity. It also has no antiestrogenic activity, which suggests that it should not antagonize estradiol's beneficial effects. This is important for its use in the treatment of endometriosis, because, due to DNG's low gonadotropic activity, E2 levels are not decreased to zero, in contrast to treatments with gonadotropin-releasing hormone (GnRH) analogues. This maintenance beneficial E2 effects is of particular importance for the general tolerability of the first contraceptive pill to use E2V instead of EE, although clinical endpoint studies are still ongoing. These studies are expected, on the basis of its pharmacology, to demonstrate the cardiovascular safety of the new pill. © 2012 Elsevier Ireland Ltd. All rights reserved.


Wu S.W.,Capital Medical University
Zhonghua fu chan ke za zhi | Year: 2010

To identify the risk factors of adverse pregnancy outcomes in expectant management of pregnant women with early onset severe pre-eclampsia (EOSP). Totally, 136 gravidas, who were diagnosed as ESOP and received expectant management from January 2007 to June 2008 in Beijing Obstetrics and Gynecology Hospital, were selected and divided into two groups: the favorable pregnancy outcome group (control, n = 101) and the adverse pregnancy outcome group (n = 35). The general clinical information, pregnancy outcomes, routine urine test, hemodynamic data, routine blood test, liver and renal function test on admission were collected and the risk factors for adverse outcomes were retrospectively analyzed. (1) General clinical information: more women complained of preeclamptic symptoms on admission in the adverse outcome group than in the control group (35.6% vs. 57.1%, P < 0.05). No significant differences was found between the two groups in the maternal age, times of previous pregnancies, prevalence of concurrent complications, pre-pregnant body mass index (BMI), proportion of women who had regular antenatal checks (P > 0.05). (2) Pregnant outcomes: the average duration of expectant management in the control group were similar to the adverse outcomes group [(6.5 +/- 8.2) days vs. (6.8 +/- 10.0) days, P > 0.05]. The main complications in the adverse outcome group included placental abruption (n = 13), heart failure and pulmonary edema (n = 10), hemolysis, elevated liver enzymes and low platelet syndrome (HELLP syndrome, n = 5), and no eclampsia was reported. However, none of these complications was reported from the control group. (3) Blood pressure and proteinuria: the gestation ages at the onset of EOSP and at delivery in the control group were earlier than those of the adverse outcome group [(31.3 +/- 3.4) weeks vs. (33.0 +/- 4.9) weeks, (32.1 +/- 3.0) weeks vs. (34.0 +/- 3.6) weeks, P < 0.05], the systolic blood pressure and urinary protein and the proportion of women with urinary protein of (+++) were also much higher in the adverse outcome group (all P < 0.05). (4) Hemodynamics and routine blood tests: the blood viscosity in the control group was obviously lower than that of the adverse outcome group (P < 0.05). But there was no significant difference in the cardiac output, cardiac index, peripheral resistance and vascular compliance between the two groups (P > 0.05). The adverse outcome group showed lower platelet (PLT) level and higher red blood cell (RBC) count and hematocrit compared with those of the control (all P < 0.01). (5) Liver and renal function: the alanine aminotransferase (ALT), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), blood urea nitrogen (BUN) in the adverse outcome group were significantly higher than those of the control group (all P < 0.05), but the plasma level of total protein (TP), albumin (Alb), uric acid (UA) and creatinine (Cr) were similar between the two groups (P > 0.05). (6) Risk factor analysis: RBC count (OR = 3.68, 95%CI: 1.90-7.13), PLT count (OR = 0.99, 95%CI: 0.98-1.00) and the gestations at delivery (OR = 0.87, 95%CI: 0.80-0.94) were the risk factors of adverse pregnancy outcomes during the expectant management of EOSP. Elevated RBC count, reduced PLT count and earlier delivery weeks are the risk factors of adverse pregnancy outcomes during the expectant management of EOSP.


BACKGROUND:: We conducted a randomized trial to evaluate the efficacy and safety of dexmedetomidine for prophylactic analgesia and sedation in patients with delayed extubation after craniotomy. METHODS:: From June 2012 to July 2014, 150 patients with delayed extubation after craniotomy were randomized 1:1 and were assigned to the dexmedetomidine group that received a continuous infusion of 0.6 μg/kg/h (10 μg/mL) or the control group that received a maintenance infusion of 0.9% sodium chloride for injection. The mean percentage of time under optimal sedation (SAS3-4), the percentage of patients who required rescue with propofol/fentanyl, and the total dose of propofol/fentanyl required throughout the course of drug infusion, as well as VAS, HR, MAP, and SpO2 were recorded. RESULTS:: The percentage of time under optimal sedation was significantly higher in the dexmedetomidine group than in the control group (98.4%±6.7% vs. 93.0%±16.2%, P=0.008). The VAS was significantly lower in the dexmedetomidine group than in the control group (1.0 vs. 4.0, P=0.000). The HR and mean BP were significantly lower in the dexmedetomidine group than in the control group at all 3 time points (before endotracheal suctioning, immediately after extubation, and 30 min after extubation). No significant difference in SpO2 was observed between the 2 groups. For hemodynamic adverse events, patients in the dexmedetomidine group were more likely to develop bradycardia (5.3% vs. 0%, P=0.043) but had a lower likelihood of tachycardia (2.7% vs. 18.7%, P=0.002). CONCLUSIONS:: Dexmedetomidine may be an effective prophylactic agent to induce sedation and analgesia in patients with delayed extubation after craniotomy. The use of dexmedetomidine (0.6 μg/kg/h) infusion does not produce respiratory depression, but may increase the incidence of bradycardia.This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially.http://creativecommons.org/licenses/by-nc-nd/4.0/. Copyright © 2015 Wolters Kluwer Health, Inc. All rights reserved


Zong H.T.,Capital Medical University
Zhonghua nan ke xue = National journal of andrology | Year: 2011

To compare the impacts of transurethral resection of the prostate (TURP), transurethral electrovaporization of the prostate (TUEVP) and holmium laser enucleation of the prostate (HoLEP) in the treatment of benign prostatic hyperplasia (BPH) on male sexual function. We identified randomized controlled trials on the influence of TURP, TUEVP and HoLEP on the sexual function of BPH patients, and performed meta-analysis on the included data using Revman 5.0.25. Nine randomized controlled trials involving 1 050 BPH patients were included in the meta-analysis. The baseline of the study was comparable. TURP affected erectile function less than TUEVP (P = 0.04), but the two had no significant difference in their influence on ejaculatory function. Nor was any significant difference found between HoLEP and TURP in their influence on either erectile or ejaculatory function at 12 and 24 months after surgery. TUEVP induces a higher incidence of erectile dysfunction than TURP, but its influence on ejaculatory function is not significantly different from the latter. HoLEP and TURP have no significant difference in their influence on erectile function and ejaculatory function.


Zhou Q.,Capital Medical University
Zhonghua fu chan ke za zhi | Year: 2010

To explore the clinical features and outcomes of patients with cerebral venous sinus thrombosis (CVT) during pregnancy and puerperium. A retrospective study was performed in 24 cases of pregnant women with CVT among 15 625 deliveries in Xuanwu Hospital from January 2002 to October 2009, including 7 cases happened during pregnancy and 17 during puerperium. The etiology, clinical presentations, imaging examination results, other relevant examinations, and pregnant outcomes of these patients were analyzed. (1) Incidence and etiology: the incidence of CVT during pregnancy and puerperium was 0.15% (24/15 652) and 29% (7/24) of the patients fell ill during pregnancy and 71% (17/24) during puerperium. Five were complicated with severe preeclampsia, while another 5 complicated with hyperemesis. One woman was complicated with anemia. CVT was identified after spontaneous delivery in 9 cases and 8 after cesarean section. (2) Clinical presentations: Among the 24 CVT cases, 22 (92%) suffered from headache, 16 (67%) reported nausea and vomiting, 15(63%) experienced hyperspasmia and 6 (25%) complained of blurred vision. On admission, 8 (33%) patients were unconscious, 3 (13%) with hemiplegia, and 8 (33%) were febrile. (3) Imaging and laboratory examinations: twelve patients underwent digital subtraction arteriography (DSA) and were diagnosed. Fifteen women showed superior sagittal sinus thrombosis in MRI and magnetic resonance intravenous angiograph (MRV). Examination of the fundus found papilledema in 4 cases. Normal cerebral sinus fluid and laboratory routine tests were reported in 13 cases, but 6 cases of hyperlipidemia, 7 cases of abnormal activated partial thromboplastin time (APTT), 5 cases of abnormal international normalized ratio (INR), 3 cases elevated platelet count, 4 cases of positive D-dipolymer, 1 cases of low hemoglobulin level (<10 g/L), 4 cases with abnormal hematocrit, and 10 cases of elevated fibrinogen (>4 g/L) were identified. (4) Management and pregnancy outcomes: among the 7 cases happened during pregnancy, 2 were complicated with severe preeclampsia and delivered through cesarean section immediately and discharged after proper management including depressurization, spasmolysis, dehydration and anticoagulant therapy. Five of the 7 cases presented with CVT during early pregnancy, among which 1 was discharged after dilation and curettage followed by anticoagulant therapy, 2 received endovascular thrombolysis after which one was discharged and the other one left with right hemiparesis and 2 patients died. Among the 17 patients presented with CVT during puerperium, 10 received anticoagulant therapy after which 4 were fully recovered, 5 left with functional disturbance (3 with hemiplegia, 1 with incomplete motor aphasia and hemiparesis and 1 with blurred vision) and one died. Among the rest 7 cases who underwent endovascular thrombolysis, 3 were fully recovered, 3 left with functional impairment (1 with blind and headache and 2 with hemiplegia), and one died. Altogether, there were 14 patients underwent systemic anticoagulant therapy after which 7 were fully recovered, 5 left with dysfunction and 2 died. Among the 10 cases received endovascular thrombolysis, 4 were fully recovered, 4 remained some dysfunction and 2 died. CVT, mostly presented as superior sagittal sinus thrombosis, are more common in puerperium than during pregnancy and DSA is the golden standard for the diagnosis of CVT. Anticoagulant therapy and endovascular thrombolysis are effective in the treatment of CVT, but may left the patients with functional disturbance or even death. Prompt diagnosis and treatment ensure a better outcome for pregnant women complicated with CVT.


To investigate safety and efficacy of dinoprostone suppositories (0.8 mm) used in cervical ripening and labor induction in women with term premature rupture of the membranes. One hundred women of term monocyesis with premature rupture of the membranes, head presentation, bishop score less than 6 (test group) and 180 women with intact fetal membranes (control group) were enrolled into this multicenter, prospective clinical study. The vaginal delivery system was inserted into the posterior fornix, and the patients were recumbent for 2 hours after insertion. The interval time from using dinoprostone suppositories to uterine contraction, to labor and delivery were recorded. The following index were also recorded and compared, including the mean inserted time of dinoprostone suppositories, fetal heart beat, meconium stained amniotic fluid, hyperstimulation of uterus and the other complications, mode of delivery, stage of labor, postpartum hemorrhage, status of neonates. Three cases in test group and 23 cases in control group weren't in labor within 24 hours. The rate of labor within 24 hours in test group was significant higher than that in control group (97.0% vs. 87.2%, P < 0.01). It was observed that 73 cases undergoing vaginal deliveries (75.3%, 73/97) and 24 cases undergoing cesarean section deliveries (24.7%, 24/97)in test group and 107 cases undergoing vaginal delivery (68.2%, 107/157) and 50 cases undergoing cesarean section delivery (31.8%, 50/157) in control group, when compared the rate of vaginal or cesarean section deliveries between two group, it didn't reach statistical difference (P > 0.05). It had no significant difference in the interval time from using dinoprostone suppositories to labor starting and the mean inserted time and the total labor time between two groups (P > 0.05). The incidence of uterine tachysystole was 11.3% (11/97) in test group and 19.1% (30/157) in control group (P > 0.05), which did not reach statistical difference (P > 0.05). There wasn't neonatal asphyxia in both groups. It was safe and efficient to use dinoprostone suppositories for cervical ripening and induction of term pregnancy with premature rupture of the membranes, however, monitoring should be intensified.


Xu A.-D.,Jinan University | Wang Y.-J.,Capital Medical University | Wang D.Z.,Illinois College
CNS Neuroscience and Therapeutics | Year: 2013

Background: The last update of the consensus statement on intravenous recombinant tissue plasminogen activator (IV rt-PA) for acute ischemic stroke (AIS) by the Chinese Stroke Therapy Expert Panel was published in 2006. Great progress has been made since then. Aim: To provide another update on the new knowledge of IV rt-PA for AIS since 7 years ago. Method: In summer of 2012, the Chinese Stroke Therapy Expert Panel was reconvened. New publications on the use of IV rt-PA for AIS were reviewed. In addition, all newly published consensus and guidelines from other countries were reviewed. The 2006 version of Chinese Consensus was then updated. Results: There is now clinical evidence to support the use of IV rt-PA between 3 and 4.5 h after the onset with several exclusion criteria. More studies are needed to provide the evidence for IV rt-PA use beyond 4.5 h. There is benefit giving IV rt-PA within 3 h to patients who are older than 80 and in patients with ongoing atrial fibrillation. Patients with INR<1.7 while on warfarin, minor strokes, rapid improving strokes and severe strokes should be treated and can all be benefited from IV rt-PA. Discussion: Since IV rt-PA was initially recommended in 1996, there is now more evidence support its use, efficacy and safety. The treatment time window is also being expanded. More public education on stroke recognition are needed so many stroke patients may benefit from the treatment. Conclusion: The 2013 version of Chinese IV rt-PA consensus contains the most up-to-date information on the use of IV rt-PA for AIS. It will be a useful tool and guideline to provide appropriate thrombolytic therapy to stroke patients who meet the criteria. © 2013 John Wiley & Sons Ltd.


Liao L.,Capital Medical University | Schaefer W.,University of Pittsburgh
International Urology and Nephrology | Year: 2014

The aim of this study was to describe typical signal patterns (TSPs) by amplitude and pressure gradients and indicate the role and significance of them in quality control. A total of 582 measurements from a multicenter urodynamic study on males (mean age, 65.3 years) were re-analyzed. Using manual graphical analysis, we identified signal patterns by typical amplitude (A) and typical pressure gradient (PG). TSPs were classified into four types: I: fine structure (A < 3 cm H2O); II: minor dynamic changes (A < 5 cm H 2O); III: major changes due to cough tests (A > 50 cm H 2O, PG > 100 cm H2O/s); IV: typical major changes due to muscular activity: detrusor overactivity (A > 3 cm H2O, PG > 1-5 cm H2O/s), rectal contractions (A = 5-10 cm H2O, PG = 5-10 cm H2O/s), and straining (A > 5 cm H2O, PG ≥ 30 cm H2O/s). At beginning of and during filling, 91.8 and 98.3 % of traces showed the identical fine structure and minor changes between P ves and Pabd tracings, and Pdet tracing was quiet. 92.3 % of Pves and Pabd traces had equal pressure changes at test coughs. During filling, 8.3 % traces showed straining, 17.4 % showed rectal contractions, and 33.7 % showed detrusor overactivity. Before voiding, 94 % of Pves and Pabd traces had equal cough response. During voiding, 91.2 % of traces showed the same fine structure, 53.3 % of traces showed straining, and 15.3 % showed relaxation of the pelvic floor. After voiding, 91.2 % of traces had the same fine structure, and 87.5 % had an equal cough response. TSPs are a powerful tool for qualitative plausibility and quality control and are an indispensable pre-condition for good urodynamic practice. © 2013 Springer Science+Business Media.


Ma J.L.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2012

To verify and assess diagnostic value of noninvasive diagnostic model of liver fibrosis in primary biliary cirrhosis (PBC) based on conventional laboratory markers. Seventy-three patients with PBC diagnosed by liver biopsy between January 2003 and June 2011 in Beijing Friendship Hospital, Capital Medical University were recruited in this study. Correlation analysis and logistic regression analysis between the conventional laboratory markers and histology stages were assessed. A liver fibrosis diagnostic model was established based upon aforementioned biomarkers and verified by its sensitivity and specificity for predicting the liver fibrosis. The predictive model (H index) consisting of five conventional laboratory markers, i.e., platelet count, serum cholinesterase, albumin, HDL-C and prothrombin time activity, could predict advanced fibrosis (stages III-IV) with an AUC(ROC) of 0.861. The sensitivity of predicting the absence of advanced fibrosis using H index < -2.20 was 96.6% and the specificity of predicting the presence of advanced fibrosis using H index > 0.41 was 93.2%. The established noninvasive diagnostic model consisting of five laboratory markers could accurately distinguish pathological changes of early stage PBC (stages I-II) from advanced stage PBC (stages III-IV).


Choi D.Y.,University of Kentucky | Zhang J.,Capital Medical University | Bing G.,University of Kentucky
Neurobiology of Aging | Year: 2010

The Lewy body is a pathological hallmark of Parkinson's disease. It has been revealed that the Lewy body contains nitrated α-synuclein which is prone to oligomerization. We tested the hypothesis that aging may enhance nitration of α-synuclein due to an exaggerated neuroinflammatory reaction such as an excessive induction of the inducible nitric oxide synthase, which occurs post-intrapallidal lipopolysaccharide (LPS) injection. Here, we show microglia activation and proinflammatory cytokine expression are more evident in the substantia nigra of elderly rats following intrapallidal LPS. In addition, greater nitration of proteins like α-synuclein occurs in the substantia nigra of 16-month-old rats versus 3-month-old rats, which is accompanied by a higher expression level of inducible nitric oxide synthase. These results imply that an exaggerated neuroinflammatory response that occurs with aging might be involved in the increase in prevalence of neurodegenerative diseases like Parkinson's disease. © 2008 Elsevier Inc.


Wang L.,Capital Medical University | Huang B.,Guilin Airforce Academy
International Journal of Antennas and Propagation | Year: 2012

A MIMO antenna composed by microstrip line-fed circular slot antenna is proposed. This antenna is used in ultra-wideband microwave imaging systems aimed for early breast cancer detection. The antenna is designed to operate across the ultra-wideband frequency band in the air. The mutual coupling between the antenna elements has been investigated to be low enough for MIMO medical imaging applications. Both the simulation and measurement results are shown to illustrate the performances of the proposed antenna. Copyright © 2012 Liting Wang and Bin Huang.


Lu S.,Capital Medical University
Journal of Spinal Disorders and Techniques | Year: 2014

OBJECTIVE:: To assess the clinical and radiographic results of activ L total disc replacement (TDR) on degenerative disc diseases (DDD).STUDY DESIGN:: A prospective clinical study.SUMMARY OF BACKGROUND DATA:: There are few reports on Activ L total disc replacement, and this is the first in China.METHODS:: From March 2009 to March 2012, 32 patients with DDD underwent either mono- or bi-segmental lumbar TDR, which was documented in a prospective observational mode. Clinical success was defined as disability(ODI) improvement of at least 15 points versus baseline, no device failure, no major complications, no neurological deterioration. Additional clinical parameters as Visual Analogue Scale (VAS), and Oswestry Disability Index (ODI) were evaluated pre- and post-operatively (1, 2, and 3 y). Radiographic parameters as range of motion (ROM), and intervertebral disc height (IDH) of the index- and adjacent segments were also carried out. Prosthesis subsidence and heterotopic ossification were observed during the follow-up period. Work status was tracked for all patients.RESULTS:: Overall, 30 patients (93.7%) were available for a mean follow-up of 28.8 months (12 mo to 46 mo) and had complete radiographic data. Their mean age was 45.1 years (32 y to 58 y). At 3 years post-operatively, the success rate was 86.7% (26/30). After surgery, clinical parameters as VAS score for back and leg pain, and ODI score showed statistically significant improvement (P<0.001), and the situation was well maintained during the follow-up time points. At 3 years post-operatively, the mean IDH at the index segment, upper and lower adjacent segments were 12.87 mm, 12.61 mm and 11.62 mm, respectively, showing no significant difference compared to preoperative data (P1=0.0597, P2=0.6669, P3=0.9813). The ROM of the index and upper adjacent segment showed a slight but signigicant increase at the 3 year’s follow-up compared to baseline (P1=0.0128, P2=0.0007). The changes of ROM at the lower adjacent segment were not significant (P=0.6637). Tears of the iliac vein were observed in two patients. Prosthesis subsidence was observed in three patients (1 at 12 M post-op, 1 at 24 M post-op, and 1 at 32 M post-op). Heterotopic ossification was observed in one patient at 36 M post-op. At the 3 year’s follow-up, only 8 patients went back to their original work, and 15 patients changed jobs, while the last 7 patients stopping working.CONCLUSIONS:: The 1- to 3-year follow-up of this cohort of patients showed satisfactory clinical outcomes. The IDHs at index- and adjacent segments were well maintained after the surgery. The ROM at the lower adjacent segment remained unchanged, but the ROM at the index and upper adjacent segment showed a slight increase. The long-term results of activ L total disc replacement was to be investigated. © 2014 by Lippincott Williams & Wilkins, Inc.


Liu F.,Texas A&M University | Wang S.,Capital Medical University
Histology and Histopathology | Year: 2014

The hypofunction of salivary glands caused by Sjögren's Syndrome or radiotherapy for head and neck cancer significantly compromises the quality of life of millions patients. Currently no curative treatment is available for the irreversible hyposalivation, whereas regenerative strategies targeting salivary stem/progenitor cells are promising. However, the success of these strategies is constrained by the lack of insights on the molecular cues of salivary gland regeneration. Recent advances in the molecular controls of salivary gland morphogenesis provided valuable clues for identifying potential regenerative cues. A complicated network of signaling molecules between epithelia, mesenchyme, endothelia, extracellular matrix and innervating nerves orchestrate the salivary gland organogenesis. Here we discuss the roles of several cross-talking intercellular signaling pathways, i.e., FGF, Wnt, Hedgehog, Eda, Notch, Chrm1/HB-EGF and Laminin/Integrin pathways, in the development of salivary glands and their potentials to promote salivary regeneration.


Wang Y.N.,Capital Medical University
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2012

To introduce the clinical manifestations and laboratory tests of adult onset of primary hemophagocytic syndrome (HPS), and to investigate the essentials of diagnosis and the genotype characteristics in adult onset patient. The definite diagnosis of HPS was made according to HLH-2004. Exons of PRF1, STX11, UNC13D, SH2D1A and RAB27A genes coding region were amplified using polymerase chain reaction. A 48-year-old man was admitted to our hospital because of recurrent fever, pancytopenia and lymph node enlargement. His laboratory test revealed bone marrow hemophagocytosis, elevated ferritin level (2000 μg/L), reduced level of NK cell activity (20.13%) and elevated soluble CD25 level (12277 U/ml). Based on the HLH-2004 diagnostic criteria, the patient was diagnosed as HPS. The patient had viral infection, and no other primary disease was identified that would cause HPS. The patient responded poorly to anti-viral therapy. DNA sequencing was used to confirm that perforin gene mutations might be one of the causes of the patient suffered from primary HPS. Although primary HPS usually affects infants and young children, it also occurred in teens and adults. It is essential to perform genetic screenings to patient whose illnesses recur with unknown causes. In addition, detection of molecular genetic alterations can be used to distinguish primary HPS from acquired HPS.


Wenming C.,Capital Medical University
Journal of Leukemia and Lymphoma | Year: 2015

2014 International Myeloma Working Group (IMWG) summit was held in Milan at June 2014. During this meeting, the criteria of asymptomatic and symptomatic multiple myeloma was revised. No benefit was shown with conventional treatment for asymptomatic patients. Frontline therapy in newly diagnosed multiple myeloma, also in elderly patients was discussed. Individualized treatment, especially for genetic risk stratification should be done, but randomized control studies needed to be done to confirm its feasibility. © 2015, Editorial Board of Journal of Leukemia and Lymphoma. All rights reserved.


Wang P.,Capital Medical University | Si T.,Peking University
Shanghai Archives of Psychiatry | Year: 2013

Summary: There is a long history of using antipsychotic medications in the treatment of depressive disorders. Atypical antipsychotics, which have fewer side effects than traditional antipsychotics, have been used as monotherapy or adjunctively with antidepressants to treat depressive disorders with or without psychotic symptoms. The antidepressant effect of atypical antipsychotics involves regulation of monoamine, glutamate, gamma-aminobutyric acid (GABA), Cortisol, and neurotrophic factors. To date, the United States Food and Drug Administration (USFDA) has approved aripiprazole and quetiapine slow-release tablets as adjunctive treatment for depressive disorders, and the combination of olanzapine and fluoxetine for the treatment of treatment-resistant depression. When using atypical antipsychotics in the treatment of depressed patients, clinicians need to monitor patients for the emergence of adverse effects including extrapyramidal symptoms (EPS), weight gain, and hyperglycemia.


Ping H.,Capital Medical University
Surgical endoscopy | Year: 2010

BACKGROUND: The Hem-o-lok polymer clip has become the preferred method to control large vessels during operation. We explored the advantages of application of the Hem-o-lok ligation system in laparoscopic nephrectomy. METHODS: From January 2004 to May 2009, 116 laparoscopic nephrectomies were performed using Hem-o-lok clips to control renal artery and vein, including 22 simple nephrectomies, 63 radical nephrectomies, and 31 nephroureterectomies. Operative time, estimated intraoperative blood loss, recovery time of intestinal function, postoperative hospital day, as well as complication rate were recorded and studied retrospectively. The number and cost of Hem-o-lok clips were analyzed. RESULTS: All 116 laparoscopic nephrectomies using Hem-o-lok clips for renal pedicle control were accomplished successfully without conversion to open surgery. No intra/postoperative vascular complications or other clip-related complications occurred. Laparoscopic operating time was 14-275 min (average 146.5 min). Estimated blood loss was 25-600 ml (average 159.2 ml). Mean recovery time of intestinal function was 30.4 h (range 16-72 h). Postoperative hospital stay was 4-22 days, with an average of 7.3 days. The mean number of Hem-o-lok clip used per operation was 5.3 (range 4-8). CONCLUSIONS: Use of the Hem-o-lok ligation system to control renal pedicle is safe and reliable for laparoscopic simple/radical nephrectomy.


Zhang J.,Capital Medical University
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2011

To evaluate the efficacy of different interventional bronchoscopic techniques for the management of benign cicatricial hyperplasia airway stenosis, and to study the factors associated with the treatment effects. From December 2004 to December 2009, 36 patients with cicatricial airway stenosis were admitted to our department. An investigation was made to analyze the effects by different interventional bronchoscopic treatments. The most suitable treatment modality for cicatricial airway stenosis was explored and described. For the 36 patients, the disease was cured in 9, improved in 10, not improved in 12, and failure in 5. The cure rate, effective rate and ineffective rate were 25%, 53% and 47%, respectively. Further analysis showed that the effective rate was 8% and 82% respectively for the electrical coagulation therapy and the balloon dilation combined with needle electrical knife and/or cryotherapy. Restenosis extension after operation occurred in 67% of the cases by electrical coagulation therapy, but only 12% of cases by the balloon dilation combined with needle electrical knife and/or cryotherapy. Our experience demonstrated that for the treatment of airway cicatricial stenosis, electrical coagulation might induce and worsen serious airway restenosis resulting in failure of treatment. On the other hand, balloon dilation combined with needle electrical knife and/or cryotherapy might be a relative safe and effective therapy for airway cicatricial stenosis.


Chen Z.,Capital Medical University
Chinese Journal of Contemporary Neurology and Neurosurgery | Year: 2016

Lumbar fusion procedures have become a necessary management strategy in the treatment of lumbar degenerative disease. The new version of the "Guidelines for the performance of fusion procedures for degenerative disease of the lumbar spine" was published by American Association of Neurological Surgeons (AANS) in 2014 since the first version had been published in 2005. Through comprehensive review of existing literatures, the new guideline analyzed current evidence of evidence-based medicine and formulated hierarchical recommendation grades from 16 topics which included assessment of functional outcome following lumbar fusion, assessment of economic outcome, radiographic assessment of fusion status, and so on, in order to provide clinical treatment guidance. This article interpreted the new guidelines summarily to help surgeons gain some understanding on it. Copyright © 2016 by the Editorial Board of Chinese Journal of Contemporary Neurology and Neurosurgery.


Cao X.,Capital Medical University | Cao X.,Beihang University | Wang N.,Beihang University
Analyst | Year: 2011

Fe 2O 3 was generally considered to be biologically and electrochemically inert, and its electrocatalytic functionality has been rarely realized directly in the past. In this work, Fe 2O 3 nanowire arrays were synthesized and electrochemically characterized. The as prepared Fe 2O 3 nanomaterial was proved to be an ideal electrode material due to the intrinsic peroxidase-like catalytic activity. The Fe 2O 3 nanowire array modified glucose sensor exhibited excellent biocatalytic performance towards the oxidation of glucose with a response time of <6 s, a linear range between 0.015-8 mM, and sensitivity of 726.9 μA mM -1cm -1. Additionally, a high sensing selectivity towards glucose oxidation in the presence of ascorbic acid (AA) and dopamine (DA) has also been obtained at their maximum physiological concentrations, which makes the Fe 2O 3 nanomaterial promising for the development of effective electrochemical sensors for practical applications. © 2011 The Royal Society of Chemistry.


Qin W.,Tianjin Medical University | Liu Y.,CAS Institute of Automation | Jiang T.,CAS Institute of Automation | Yu C.,Tianjin Medical University | Yu C.,Capital Medical University
PLoS ONE | Year: 2013

Visual experience plays an important role in the development of the visual cortex; however, recent functional imaging studies have shown that the functional organization is preserved in several higher-tier visual areas in congenitally blind subjects, indicating that maturation of visual areas depend unequally on visual experience. In this study, we aim to validate this hypothesis using a multimodality MRI approach. We found increased cortical thickness in the congenitally blind was present in the early visual areas and absent in the higher-tier ones, suggesting that the structural development of the visual cortex depends hierarchically on visual experience. In congenitally blind subjects, the decreased resting-state functional connectivity with the primary somatosensory cortex was more prominent in the early visual areas than in the higher-tier ones and were more pronounced in the ventral stream than in the dorsal one, suggesting that the development of functional organization of the visual cortex also depends differently on visual experience. Moreover, congenitally blind subjects showed normal or increased functional connectivity between ipsilateral higher-tier and early visual areas, suggesting an indirect corticocortical pathway through which somatosenroy information can reach the early visual areas. These findings support our hypothesis that the development of visual areas depends differently on visual experience. © 2013 Qin et al.


Tan K.,Capital Medical University
The Tohoku journal of experimental medicine | Year: 2011

There is no reliable way to identify the high-risk patients with intermediate coronary artery lesions (diameter stenosis 20%-70%) in early stage. Soluble CXC chemokine ligand 16 (CXCL16) is a newly discovered chemokine that can mediate inflammatory responses. It is released by proteolytic cleavage of its membrane-bound form, named scavenger receptor for phosphatidylserine and oxidized lipoprotein (SR-PSOX) that can promote the uptake of oxidized low-density lipoprotein cholesterol by macrophages. We have hypothesized that CXCL16 is an indicator of the prognosis of intermediate coronary artery lesions, and thus assessed the association between plasma CXCL16 concentrations and the 2-year prognosis in 616 patients with intermediate coronary artery lesions. The primary endpoint was a composite of all-cause death, non-fatal myocardial infarction, revascularization and angina pectoris requiring re-hospitalization. During the median follow-up time of 24 months, 69 events occurred. The plasma concentrations of CXCL16 (median 7712.88 pg/ml vs. 6792.43 pg/ml, P = 0.014) and high-sensitivity C-reactive protein (hs-CRP) (median 2.82 mg/L vs. 1.68 mg/L, P < 0.001) were higher in patients with events than patients without events. Cox hazard proportion analysis showed patients in upper CXCL16 quartile were more likely to suffer from adverse outcome than patients in lower quartile (RR = 1.271, P = 0.029, 95% CI: 1.025-1.577) after adjusting for sex, age, smoking, hypertension, diabetes, fat, dyslipidemia, hs-CRP, and medication use. In conclusion, plasma level of CXCL16 is an independent predictor of the prognosis of the patients with intermediate coronary lesions. Elevated plasma CXCL16 is associated with higher risk for these patients.


Ding T.,Capital Medical University
Journal of Leukemia and Lymphoma | Year: 2015

This article focuses on the rapidly evolving understanding of the molecular pathogenetic mechanisms of the bcr-abl-negative myeloproliferative neoplasms (MPN) [myeloproliferative disorders (MPD)], such as polycythemia vera (PV), essential thrombocythemia (ET), and primary myelofibrosis (MF). The amplify therapies were reviewed and IFN- a is an effective agent for these MPN (MPD). Also, the article emphasize once again avoidance MPN and select MPD for such chinese patients. © 2015 by the Chinese Medical Association.


Du J.,Capital Medical University
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2011

The purpose of the present study is to observe the effect of electro-acupuncture (EA) stimulation on intracerebral neurotransmitters in a rat model of Parkinson's disease (PD), and explore the possible mechanism. We used 6-hydroxydopamine (6-OHDA) injection in medial forebrain bundle (MFB) in the right brain of Sprague Dawley (SD) rat to establish the parkinsonian rat model, and randomly divided the PD rats into model and 100 Hz EA stimulation groups (n =10 in each group). EA stimulation group received 4 courses of EA stimulation on Baihui (GV-20) and Dazhui (GV-14) acupuncture points. Moreover, ten rats were randomly selected as sham operation group, only receiving normal saline (NS) injection in MFB. Then apomorphine (APO)-induced rotational behavior in different groups was recorded, and the contents of γ-aminobutyric acid (GABA) in the brain were analyzed with high pressure/performance liquid chromatography-electrochemical detection (HPLC-ECD). The results showed that model group exhibited abnormal rotational behavior with APO treatment, suggesting the successful establishment of PD model. Compared with sham operation group, model group showed increased GABA contents in cortex and striatum, as well as decreased GABA content in ventral midbrain, on the lesioned side. EA stimulation could effectively ameliorate the abnormal rotational behavior of PD rat. Compared with the model group, EA stimulation decreased the ratio of GABA content on the lesioned side to that on unlesioned side in the cortex, while increased the ratios in the striatum and cerebellum. However, there was no difference of the ratio in the ventral midbrain among three groups. These results suggest high-frequency EA stimulation significantly improves the abnormal behavior of PD rats, which may exert through enhancing the inhibitory effect of cerebellum-basal ganglia-cortical loop on motor center.


Ding C.H.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2010

To summarize the clinical characteristics of acute pandysautonomia in childhood, to gain better understanding of the diagnosis and differential diagnosis. The clinical data of 6 children with acute pandysautonomia were analyzed and followed-up. All the 6 patients had routine blood and cerebrospinal fluid (CSF), electrocardiography (ECG), electromyography (EMG), cranial magnetic resonance imaging (MRI) and autonomic nerve function tests (head upright tilt test, dermatography test, and thermal/sympathetic sweat response). Other laboratory examinations such as immunologic markers of CSF, electroencephalography (EEG), spinal cord MRI and somatosensory evoked potential (SEP) were also performed in some patients. Of the 6 patients, 1 was male, and 5 were female. The age of onset was from 2.3 to 14.5 years (average 8.2 years). The initial symptoms were gastrointestinal dysfunction in 3 patients and somatic motor dysfunction as their initial symptoms, one had irritability in 1 case, pain in 1 and dysphagia in 1, respectively. Autonomic nerve signs and symptoms: (1) Skin and mucosa are rough and dry, there was no or little perspiration, alacrimia or little tear in all patients. (2) Vision problem appeared in 1 patient, blepharoptosis in 3 patients, pupillary abnormality existed in all patients. (3) Gastrointestinal symptoms were present in all patients. Vomiting and constipation were present in 4 patients, diarrhea and constipation were alternatively present in 1 patient, abdominal distention and abdominal pain were present in 2 patients. (4) Cardiovascular system manifestations included postural dizziness or syncope in 3 patients, tightness and palpitation in 2 patients. (5) Urinary dysfunction was present in 4 patients. In addition, mild to moderate somatic motor dysfunction was present in 5 patients, sensory dysfunction in 3 patients. Autonomic nerve function tests were abnormal in all patients. Laboratory findings included serum IgM antibody to herpes simplex virus and antistreptolysin "O" (ASO) test were positive respectively in 1 patient. The immunological markers in CSF were abnormal in 3 patients and the protein level in CSF was slightly elevated in 3 patients. Cranial MRI was slightly abnormal in 4 patients. ECG was slightly abnormal in all patients. EMG was abnormal in 5 patients. SEP was abnormal in 3 patients. Five patients received IVIG therapy. Five patients were followed-up. One patient died, one lost to follow up and one had slight improvement. Significant improvement was seen in 2 patients. Acute pandysautonomia in children usually had non-specific symptoms and could affect multiple organs. Heterotropia, cardiovascular dysfunction and gastrointestinal dysfunction were commonly seen in these patients. In acute pandysautonomia patients, IVIG seemed to be effective and the prognosis was poor in severe cases.


Dai Y.F.,Capital Medical University
Yi chuan = Hereditas / Zhongguo yi chuan xue hui bian ji | Year: 2011

Familial hypercholesterolemia (FH), one monogenic autosomal dominant disease, mainly results from genetic defects in the low-density lipoprotein receptor (LDLR) gene, which leads to the reduction or absence of cell surface LDLR, disorder of cholesterol metabolism, and cholesterol deposition in different tissues and organs. FH is a common metabolic disease clinically characterized by the presence of xanthomas and premature coronary heart disease. To date, about 1 741 variants have been identified in gene LDLR, among which 108 variants were identified in Chinese FH patients. To better understand the features of LDLR gene mutations and help to FH diagnosis and therapy, this review provides a comprehensive overview of LDLR gene mutations in Chinese FH patients.


Benzene is an established haematotoxic and genotoxic carcinogen. DNA methyltransferase inhibitor, 5-aza (5-aza-2'-eoxycytidine) and histone deacetylase inhibitor, TSA (trichostatin A) are two kinds of key epigenetic modification reagents. Although apoptosis has been considered as the key cytotoxicity mechanism, the effects of these epigenetic reagents on benzene-induced apoptosis have not been reported. In this study, BMCs (bone marrow cells) from rats were incubated with benzene and then with either 5-aza, TSA alone or the combination of the two drugs. Apoptosis and mRNA expression were detected by annexin V/PI (propidium iodide) staining assay and real-time PCR, respectively. Results showed that benzene caused cell apoptosis accompanied with bcl-2 mRNA decrease, caspase-3 and bax mRNA increase. Moreover, benzene-induced apoptosis and the decrease of bcl-2 mRNA were both reversed by both 5-aza and TSA, but the role of TSA was significantly larger than 5-aza. More interestingly, these increases in benzene-induced caspase-3 and bax mRNA expression were obviously suppressed by 5-aza but not by TSA. In conclusion, 5-aza inhibited benzene-induced apoptosis through down-regulating of caspase-3 and bax and up-regulating bcl-2 mRNA expression, whereas the effect of TSA on apoptosis dominatingly affected bcl-2 mRNA expression, and 5-aza together with TSA had no synergic effect on benzene-induced apoptosis.


Zhang X.,Capital Medical University
Sheng li xue bao : [Acta physiologica Sinica] | Year: 2011

Antibody against the angiotensin AT1 receptor (AT1-Ab) could disturb placental development. The placenta is the key organ between mother and fetus. Placental damage will seriously impair fetal growth and development in utero, leading to intrauterine growth restriction (IUGR). Based on the fetal origins of adult disease (FOAD) hypothesis, IUGR could increase a propensity to develop adult onset cardiovascular disease (CVD). The present study was designed to determine whether vascular function has changed in the adult offspring of AT1-Ab positive pregnant rats. Twenty four female rats (8-week-old, AT1-Ab negative) were randomly divided into two groups, immunized and vehicle groups. Immunized group received active immunization to establish AT1-Ab-positive model, while vehicle group was subjected to Freund's adjuvant without antigen. After 8 weeks of immunization, the antibody titers in sera from the female rats were detected by enzyme-linked immunosorbent assay (ELISA). Then all the female rats were mated with normal Wistar male rats and became pregnant. Immunized/vehicle group offspring rats (I offspring/V offspring) were raised to 40-week-old under standard chow feeding. Then the two groups' offspring rats were given a high-salt diet for 12 weeks (4% NaCl in chow feeding). Systolic blood pressure (SBP) was measured dynamically by noninvasive blood pressure system. The vascular ring experiment was performed to detect vascular function and reactivity. As detected by ELISA, the titers of antibody peaked at the 8th week (OD values: 2.75 ± 0.08 vs 0.33 ± 0.01, P < 0.01 vs vehicle group at the same time point). There was no significant difference of SBP between the two groups' offspring rats during the high-salt diet (P > 0.05). Isolated thoracic aortic rings of I offspring had significantly decreased constriction under norepinephrine treatment (P < 0.01 vs V offspring) and significantly decreased dilation under acetylcholine treatment (P < 0.05 vs V offspring). These results suggest that the offspring of AT1-Ab-positive pregnant rats are more susceptible to vascular functional abnormality while being fed high-salt diet.


Guo X.,Capital Medical University
BMC public health | Year: 2014

BACKGROUND: Cervical cancer is the second most common cancer and cause of cancer-related death for women worldwide. The aims of this study were to investigate the prevalence of cervical neoplasia and examine factors associated with high-grade cervical squamous intraepithelial lesions (HSIL) among women taking part in a cervical cancer screening program in Beijing.METHODS: Women aged 25-65 years were screened using the ThinPrep cytologic test and gynecologic examination. Univariate and multivariate logistic regressions were conducted to investigate factors associated with HSIL.RESULTS: Among 728,704 women screened, the prevalence of cervical intraepithelial neoplasia (CIN) I, II, III was 50.2, 34.0, and 36.4 per 100,000, respectively. Prevalence of cervical cancer was 12.2 per 100,000. Risk factors for HSIL included being in age group of 46-55 years (adjusted odds ratio [aOR] = 1.15, 95% CI: 1.07-1.44, compared with the 25-35 age group), bleeding after intercourse (aOR = 2.08, 95% CI: 1.40-3.10), and presence of trichomonas vaginalis infection (aOR = 2.62, 95% CI: 1.35-5.07), cervical inflammation (aOR = 4.22, 95% CI: 3.39-5.26), and genital warts (aOR = 3.89, 95% CI: 2.54-7.70). High education level (college and above compared with junior middle school or lower) was found to be protective (aOR = 0.79, 95% CI: 0.37-0.90).CONCLUSIONS: The prevalence of cervical neoplasia is relatively high in Beijing. Women aged 46-55 years, those with a lower education level, those reporting bleeding after intercourse, and those affected by Trichomonas vaginalis infection, cervical inflammation and genital warts are at higher risk for HSIL. Particular efforts should be made to ensure these women are included in cervical cancer screening programs.


Cui X.,Capital Medical University | Rockett I.R.H.,West Virginia University | Yang T.,Zhejiang University | Cao R.,Institute of Health Education
BMC Public Health | Year: 2012

Background: Stimulated by rapid modernization and industrialization, there is massive rural-urban migration in China. The migrants are highly susceptible to smoking and mental health problems. This study examined the association between both perceived work stress and perceived life stress with smoking behavior among this group during the period of migration. Methods. Participants (n = 1,595) were identified through stratified, multi-stage, systematic sampling. Smoking status separated non-smokers from daily and occasional smokers, and migration history, work stress, and life stress were also measured. Analyses were conducted using the Chi-square test and multiple logistic regression. Two models were utilized. The first was the full model that comprised sociodemographic and migration-related characteristics, as well as the two stress variables. In addressing potential overlap between life and work stress, the second model eliminated one of the two stress variables as appropriate. Results: Overall smoking prevalence was 64.9% (95% CI: 62.4-67.2%). In the regression analysis, under the full model, migrants with high perceived life stress showed a 45% excess likelihood to be current smokers relative to low-stress counterparts (OR: 1.45; 95% CI: 1.05 - 2.06). Applying the second model, which excluded the life stress variable, migrants with high perceived work stress had a 75% excess likelihood to be current smokers relative to opposites (OR: 1.75; 95% CI: 1.26-2.45). Conclusions: Rural-urban migrant workers manifested a high prevalence of both life stress and work stress. While both forms of stress showed associations with current smoking, life stress appeared to outweigh the impact of work stress. Our findings could inform the design of tobacco control programs that would target Chinese rural-urban migrant workers as a special population. © 2012 Cui et al; licensee BioMed Central Ltd.


Xu X.X.,Capital Medical University
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology | Year: 2010

To determine whether tanycytes be able to support the regeneration of completely transected spinal cord in adult rats. Subcultured tanycytes was transplanted into completely T8 transected spinal cord using the untranslated completely transected rats as control. After transplantation the rubrospinal motor evoked potentials were recorded below the injury level at the end of 12th week, assistant by Basso-Beatie-Bresnahan locomotor rating scale and histology method. At the end of 12th week the total peak amplitude of rubrospinal motor evoked potentials (MD = 133.2 microV, P < 0.01) and BBB locomotor rating scale (MD = 5.0000, P < 0.01) were both significantly improved in cell transplanted group compared with that in the untranslated control group, while the latency of the first peak was shortened (MD = 0.061 ms, P = 0.040). HE staining showed more integrity in transected spinal cords in cells transplanted groups. Transplanted tanycytes can support the regeneration of transected spinal cords in rats.


Fang J.G.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To investigate the indications and complications of the total thyroidectomy in the management of thyroid cancer. Fifty-one patients with thyroid cancer operated with total thyroidectomy in a period from April 2007 to June 2010, were retrospective analyzed. There were 48 cases of papillary adenocarcinoma, 1 follicular adenocarcinoma, and 2 medullary carcinoma as well, 2 cases with hyperthyroidism, 3 cases with remote metastasis. There were 17 males and 34 females who in their age ranged from 5 years old to 82 years old with a middle age of 50 years old. Among the 45 cases who were initial surgical management patients, there were 17 T1, 13 T2, 7 T3, 8 T4; and there were 31 N0, 11 N1a, 3 N1b, 2 M1. The recurrent laryngeal nerve and the parathyroid gland, and supper laryngeal nerve were exposed routinely. Lymph nodes in Level VI were dissected in all patients, and Level II-IV dissected in 25 patients. 131I were administrated in 8 patients. Patients were followed up from 1 month to 36 months. There were no patients died and lost of follow up. The two years survival rate was 100.0% (15/15). Pathological examination showed that the multiple focal disease rate was 42.2% (19/45), the cervical lymph nodes metastases rate was 51.0% (26/51). There was no permanent paralysis of recurrent laryngeal nerve and outer branch of supper laryngeal nerve. The permanent hypoparathyroidism rate was 3.9% (2/51). One case with contralateral lymph node metastasis was reoperated and survived without disease. Two cases with remote metastasis were alive with steadied disease. Under the skilled hand, total thyroidectomy is a safe procedure in the management of thyroid cancer. Totally exposing the parathyroid gland and laryngeal nerve is the key point to prevent the major complications.


Chen W.,Beijing University of Chinese Medicine | Yang G.-y.,Beijing University of Chinese Medicine | Liu B.,Capital Medical University | Manheimer E.,University of Maryland Baltimore County | Liu J.-P.,Beijing University of Chinese Medicine
PLoS ONE | Year: 2013

Objective:Manual acupuncture has commonly been used in China, either alone or in combination with conventional medicine, to treat diabetic peripheral neuropathy (DPN). The objective of this study was to perform a systematic review to evaluate the potential benefits and harms of manual acupuncture for DPN to justify its clinical use.Methods:We searched for published and unpublished randomized controlled trials of manual acupuncture for DPN till 31 March 2013. Revman 5.2 software was used for data analysis with effect estimate presented as relative risk (RR) and mean difference (MD) with a 95% confidence interval (CI).Results:A total of 25 trials involving 1649 participants were included. The methodological quality of included trials was generally poor. Meta-analysis showed that manual acupuncture had better effect on global symptom improvement compared with mecobalamin (RR 1.31, 95%CI 1.21 to 1.42), vitamin B1 and B12 (RR 1.55, 95%CI 1.33 to 1.80), and no treatment (RR 1.56, 95%CI 1.31 to 1.85), and that the combination of manual acupuncture and mecobalamin had better effect compared with mecobalamin alone on global symptom improvement (RR 1.56, 95%CI 1.28 to 1.90). Adverse events were not reported in any trials. The asymmetric funnel plot suggested publication bias.Conclusions:Despite the number of trials of manual acupuncture for DPN and their uniformly positive results, no clinically relevant conclusions can be drawn from this review due to the trials' high risks of bias and the possibility of publication bias. Clearly defined and internationally acknowledged outcome measures are required for future study. There remains an urgent need for training Chinese researchers in conducting unbiased trials as well as prospectively registering all initiated Chinese trials to avoid publication bias. © 2013 Chen et al.


Liu P.,Capital Medical University
Neurosurgery | Year: 2010

Cerebrospinal fluid (CSF) rhinorrhea is leakage of CSF from the nasal cavity caused by cranial base or meningeal defects. Surgical treatment of CSF rhinorrhea is still problematic. We evaluated the clinical outcomes of 132 consecutive cases of CSF rhinorrhea treated via transcranial or transnasal endoscopic approaches according to the patient's condition. The indications for the approaches are discussed. Of 132 patients with CSF rhinorrhea, a transnasal endoscopic approach was used in 98 to repair cranial base defects in the ethmoid and sphenoid sinuses. A transcranial intradural approach was used in the remaining 34 patients for frontal sinus defects, multiple fractures of the cranial base, or combination nerve injury. CSF rhinorrhea resolved after initial surgery in 124 of 132 patients, giving a success rate of 94%. Of the 8 failures or recurrent cases, 4 were successfully repaired by repeat endoscopic surgery, 2 were cured by transcranial revision surgery, and 2 refused additional surgery (the condition subsequently resolved without treatment in these patients). Postoperative complications included intracranial infection (8 patients) and anosmia (1 patient). No neurological deficits were apparent over the 10-month mean follow-up period. Transnasal endoscopic repair is a reliable method for CSF rhinorrhea patients whose fistulae are located in the ethmoid and sphenoid sinuses. The transcranial procedure should be the treatment of choice for patients with frontal sinus fracture, multiple or complex anterior cranial base fractures, or nerve injury. A satisfactory surgical outcome depends on exact diagnosis, proper operative approach, and the surgeon's skill and experience.


Yu B.,Capital Medical University
Chinese Journal of Pathology | Year: 2010

Objective To clarity clinical and morphological teatures and immunophenotype of T lymphoblastic lymphoma/leukaemia ( T-LBL/ALL ) and to further improve the knowledge and diagnostic accuracy for T-ALL/LBL. Methods 128 cases of T-LBL/ALL were analyzed for the clinical features, morphology, immunophenotype and TCR gene rearrangement using routine eosin and haematoxylin stain, immunohistochemistry and polymerase chain reaction combining with the clinical findings. Results In 128 cases of T-LBL/ALL, there were 94 male and 34 female. The ratio of male/female was 2. 8:1. The age of patients ranged from 4 to 88 years, with an average of 27 years and a median of 22 years. Lymph nodes and extranodal areas were involved in 58/128 and 27/128 cases of T-LBL/ALL, respectively. The other 43 cases had involvement of both nodal and extranodal areas. Cervical node and mediastinum were involved in 74 cases and 43 cases, respectively. Diffuse growth pattern of tumor cells was predominant. Nodular growth pattern was seen only in a few cases. Most cases composed of small to medium-sized lymphoblasts, and other 7 cases showed a composition of large lymphoblasts. Tumor cells expressed TdT in 121/128 (94. 5%) cases, CD34 in 48/98(49.0%)cases, CD3 in 78/108(72. 2% ) cases, CD7 in 104/108(96. 3%) cases, CD43 in 56/63(88.9% ) cases, CD79a in 5/70(7.1% ) cases, CD10 in 25/76 (32.9% ) cases, CD99 in 58/60 (96.7% ) cases and Pax-5 in 4/91 (4.4% ) cases. All of the cases were negative for MPO. A follow up data, ranging from 1 to 53 months, was obtained in 51/128 (39. 8% ) patients. The over all survival rate was 68. 6% and the median survival time was 12 months. Under a similar condition of carrying a positive staining result on CD3 in tumor cells, there was a statistically significant difference between patients in the group of over 30 of age and that with the age ranging from 11 to 30. Patients associating with a CD10 positive staining of tumor cells showed also a shorter survival period. In addition, there were 4 out of 5 cases showing the presence of TCR gene rearrangement. Conclusions T-LBL/ALL are aggressive in behavior, associating mainly with enlarged cervical lymph nodes and masses in the mediastinum, occurring predominantly in children and young adults. Although small to medium-sized tumor cells with diffuse pattern were found in most cases, however, large-sized tumor cells and nodular pattern could also be obtained in a few cases. Immunohistochemistry staining particularly adoption of CD7, Pax-5, TdT, CD34 and Ki-67 stainings in combination are helpful of making a diagnosis for T-LBL/ALL. Aanalysis of TCR gene rearrangement will be helpful for the diagnosis of a few difficult cases.


Hong J.,Capital Medical University
Echocardiography (Mount Kisco, N.Y.) | Year: 2013

The aim of this study was to assess left atrial (LA) functional remodeling in lone atrial fibrillation (LAF) patients via two-dimensional speckle tracking echocardiography (2DSTE). Forty-five paroxysmal LAF patients and 30 healthy controls underwent 2DSTE in sinus rhythm. Longitudinal LA strain/strain rate (LAS/LASR) of booster, reservoir, and conduit function in apical four- and two-chamber views were measured and averages of the 2 views were used. The 2 groups had similarity in LA volume. LAS and LASR of the 3 phasic functions were reduced in patients. Comparing the 2 groups, LA booster strain (LASa) and strain rate were -12.31 ± 3.15 versus -15.30 ± 2.52% and -1.64 ± 0.51 versus -2.08 ± 0.48 1/sec, respectively, P < 0.05 for both. Multivariate regression confirmed that LAF was an independent determinant of LAS/LASR reduction. LASa was the most accurate parameter for differentiating LAF patients from controls (area under receiver operating characteristic curve, 0.763; cutoff value, -14.3%; sensitivity, 71.1% and specificity, 70.0%). Finally, LASa decrement was found to be the only significant predictor of early atrial fibrillation (AF) recurrence (odds ratio [OR], 1.39 per unit decrement; 95% CI, 1.02-1.89; P = 0.038). Remodeling of LA booster pump, reservoir, and conduit function in LAF patients can be detected by 2DSTE before structural remodeling. Occurrence of LAF is associated with impairment of LA intrinsic myocardial properties. LASa can differentiate LAF patients and lower LASa is associated with early recurrence of AF after ablation. © 2013, Wiley Periodicals, Inc.


Shen C.,Peking University | Shen C.,Capital Medical University | Zhong N.,Peking University | Zhong N.,New York State Institute for Basic Research in Developmental Disabilities
American Journal of Reproductive Immunology | Year: 2015

Long non-coding RNAs (lncRNAs) are long single-stranded RNAs without translation potential. LncRNAs function in regulating epigenetic and cellular processes through various mechanisms. Nowadays, rapidly growing evidence has shown that abnormally expressed lncRNAs were involved in various inflammation-related states or diseases. Abnormal inflammation responses contribute to reproductive pathology and play vital roles in developing most disorders of the female reproductive system. In this review, we discussed the history of ncRNAs including lncRNAs, methodologies for lncRNA identification, mechanisms of lncRNA expression and regulation and mainly discussed the expression and function of lncRNAs in the female reproductive system with special focus on the inflammation and infection pathway. By analyzing the present available studies of lncRNA transcripts within the reproductive system and the current understanding of the biology of lncRNAs, we have suggested the important diagnostic and therapeutic roles of lncRNAs in the etiology of reproductive disorders. © 2014 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.


An L.X.,Capital Medical University
Zhen ci yan jiu = Acupuncture research / [Zhongguo yi xue ke xue yuan Yi xue qing bao yan jiu suo bian ji] | Year: 2010

To observe the effect of electroacupuncture (EA) of different acupoint groups on the sevoflurane anesthetic requirement for resection of supratentorial tumors and the speed of post-operation recovery. A total of 120 cases of supratentorial tumor resection patients were randomly and equally allocated into general anesthesia (GA) group, EA-proximal acupoints group (EA-PA group) and EA-distant acupoints group (EA-DA group). The supratentorial tumor patients were anesthetized with sevoflurane for surgery. Proximal acupoints used were Fengchi (GB 20), Tianzhu (BL 10), Cuanzhu (BL 2) and Yuyao (EX-HN 4) that the former 2 acupoints and the latter 2 acupoints were for penetrative needling; and distant acupoints used were Hegu (LI 4), Waiguan (TE 5), Jinmen (BL 63), Taichong (LR 3), Zusanli (ST 36) and Qiuxu (GB 40) on the craniotomy side, and stimulated with EA (2 Hz/100 Hz, a tolerable electric stimulating strength), beginning from the anesthesia induction till the end of surgical operation. All patients were anesthetized by inhalation of propofol (2 mg/kg), sufentanil (0.3 microg/kg) and vecuronium bromide (0.1 mg/kg) and maintained with sevoflurane. The end-tidal sevoflurane concentration, minimum alveolar concentration (MAC), bispectral index (BIS), and the speed of recovery were recorded. In comparison with the GA group, the end-tidal concentrations during anesthesia maintenance at the time-points of post skull drilling, dura incising, intracranial operation in EA-PA group, the time-points of post skull drilling, skull opening, dura incising, and intracranial operation in EA-DA group, and the MAC of sevoflurane at the time-points of skull drilling in EA-DA group, and skull opening, dura incising, intracranial operation and dura suturing in both EA-PA and EA-DA groups were significantly lower (P < 0.05, P < 0.01). Compared to the GA group, the BIS values of EA-DA group at the time-points of skull drilling and opening, and dura incising were significantly higher (P < 0.05), while during the recovery stage of anesthesia, in comparison with the GA group, the autonomous respiration recovery time, tracheo-tube removing time, eye-opening time, voluntary motion recovery time, orientation force recovery time, and operating room-departure time of both EA-PA and EA-DA groups were significantly shorter (P < 0.05, P < 0.01). No significant differences were found between the EA-PA and EA-DA groups in the aforementioned indexes (P > 0.05). EA of both proximal and distant acupoints can reduce the expired concentration and MAC of sevoflurane during anesthesia maintenance, and accelerate the recovery after cease of anesthesia in supratentorial tumor resection patients.


Zhang H.M.,Capital Medical University
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2011

To explore the impacts of acupuncture and rehabilitation on post-stroke abnormal patterns of limb movement and evaluate them via rehabilitation method. Ninety cases of post-stroke movement disorder were randomly divided into an acupuncture-rehabilitation group, a body acupuncture group and a medication group, 30 cases in each group. In medication group, the conventional medication in neurological department was administered. In acupuncture-rehabilitation group and body acupuncture group, on the basis of the therapy as medication group, scalp acupuncture (such as parietal area and anterior parietal area, etc.), rehabilitation training and traditional body acupuncture [such as Jianyu (LI 15) and Fengshi (GB 31),etc.] were supplemented. The continuous electric stimulation was applied in body acupuncture group. The treatment lasted for 8 weeks. The assessment of clinical efficacy, Fugl-Meyer score, Modified Ashworth scale (MAS), range of motion (ROM) and shoulder pain score were taken as observation indices for rehabilitation evaluation before and after treatment in each group. The effective rate was 93.1% (27/29) in acupuncture-rehabilitation group, which was superior to 66.7% (20/30) in body acupuncture group and 57.1% (16/28) in control group (both P<0.01) separately. After treatment, Fugl-Meyer score, MAS, ROM of the lower limbs and shoulder joint and shoulder pain score (except medication group) were all remarkably improved as compared with those before treatment in each group (all P<0.01). The improvements in Fugl-Meyer score, MAS, ROM of the upper limbs and shoulder pain score in acupuncture-rehabilitation group were significantly superior to those in body acupuncture group and medication group (P<0.05, P<0.01). Acupuncture and rehabilitation therapy and traditional body acupuncture remarkably improve in post-stroke movement disorder. But acupuncture and rehabilitation therapy is apparently superior to traditional body acupuncture. This therapy can effectively prevent and treat post-stroke abnormal patterns and it is greatly significant in the improvement of survival quality for the patients.


Zhang S.S.,Capital Medical University
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2010

To verify the clinical efficacy of Chinese Medicine syndrome-differentiation therapy in treating diarrhea-predominant irritable bowel syndrome IBS-D. With a blinded randomized controlled design adopted, 360 patients with IBS-D were randomly assigned to two groups, the treated group and the control group, they were treated with Chinese medicine and Pinaverium bromide for four weeks respectively. Comprehensive evaluation showed that the total effective rate in the treated group was higher than that in the control group significantly (93.8% vs 81.3%, P<0.01). Efficacy assessment on symptoms (by scoring) showed that the efficacy in the treated group was better than that in the control group in aspects of improving abdominal pain (86.1% vs 70.3%), defecation coziness (involving the frequency of defecation, incidence of tenesmus in the latest 10 days and Bristol typing of stool characters), living interfering, and total BSS score (P<0.05 or P<0.01). Chinese medicine syndrome-differentiation dependent therapy shows good efficacy in treating IBS-D.


Ruan W.,China Agricultural University | Wu Y.,Capital Medical University | Zheng S.J.,China Agricultural University
Molecular Biology Reports | Year: 2012

Toll-like receptors (TLRs) mediate immune response via recognition of pathogen-associated molecular patterns (PAMPs), thus play important roles in host defense. Polymorphisms of TLR5 may affect their recognition of bacterial flagellin, leading to varied host resistance to pathogenic infections. Here, we cloned TLR5 genes from Common Pheasant, Guinea fowl and 9 Chicken breeds and analyzed their sequences. The open reading frames of TLR5 were sequenced. Amino acid analysis indicated that TLR5 from Chicken breeds shared 99.4-99.9% homology. The amino acid homology of TLR5 ranged from 92.1 to 92.5% between Chickens and Guinea fowl, 95.7-96.1% between Chickens and Turkey, 94.3-94.7% between Chickens and Common Pheasant, and 79.9-80.1% between Chickens and Zebra-finch. Different genetic patterns were determined among Chickens, Common Pheasant, Guinea fowl, Turkey and Zebra-finch. It was found that there were 92 amino acid polymorphic sites, among which 5 sites in chicken TLR5, 63 sites in Guinea fowl TLR5 and 44 sites in Common Pheasant TLR5. Our data indicate that the positive Darwinian selection occurred in avian TLR5 genes since frequency of non-synonymous (dN)>frequency of synonymous (d S). These results also demonstrate that avian TLR5 genes are polymorphic among avian breeds, suggesting a varied resistance among breeds of avian. This information might be of help to improve the health of avian by breeding and vaccination. © Springer Science+Business Media B.V. 2011.


Zhao W.-H.,Capital Medical University | Gou B.-D.,China Pharmaceutical University | Zhang T.-L.,China Pharmaceutical University | Wang K.,China Pharmaceutical University
Journal of Cellular Biochemistry | Year: 2012

Vascular calcification (VC) is frequent prevalence in patients with chronic kidney disease (CKD) and atherosclerosis. Lanthanum carbonate is used as an orally administered phosphate-binding agent to reduce the gastrointestinal absorption of phosphate and ameliorate VC in advanced CKD. In this study, we used bovine vascular smooth muscle cells as a model VC in vitro and studied the effects of lanthanum chloride on calcium deposition. Exposure of cells to LaCl 3 at the concentration of 0.1μM suppressed the β-glycerophosphate-induced alkaline phosphatase activity and calcium deposition. Furthermore, LaCl 3 upregulated the β- glycerophosphate-suppressed expression of calcium-sensing receptor. In contrast to the inhibitory effect of LaCl 3 on calcium deposition, higher level lanthanum (50μM) was found to promote immediately precipitation of calcium phosphate in cell culture medium. At this concentration, LaCl 3 was found to induce cell apoptosis which involves caspases-9 and -3. These data indicate that the promotory effect of LaCl 3 on calcium deposition is likely mediated by induction of apoptosis. Our in vitro findings do suggest that, in the context of raised lanthanum, greater attention should be paid to potential toxic effects associated to the use of lanthanide-based drugs. © 2011 Wiley Periodicals, Inc.


Jin R.,Capital Medical University
Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology | Year: 2011

To compare the efficacy and safety of interferon a-1b and interferon a-1b combined with lamivudine in the treatment of HBeAg positive chronic hepatitis B (CHB), to analyze the impact of variable factors on the efficacy, and to investigate the individualized anti-viral regimen for CHB patients. 111 CHB patients were enrolled and randomly divided into two groups. Group A: patients received interferon a-1b (49 patients, 50mug I. M. , qod. ) , Group B: interferon a-1b (idem) combined with lamivudine for 6-12 months or longer(62 patients, 100 mg, P.O. , q.d. ). (1) The HBeAg seroconversion rates of treatment by 12 and 18 months were 28.6% and 36.7% in group A, 29.0% and 38.7% in group B, respectively, no significant difference found between the two groups at the end of treatment (x2=0.003, P value is more than 0.05; x2=1.500, P value is more than 0.05). (2) The HBV DNA undetectable rates of treatment by 6 months, 12 months and 18 months were 8.2%, 53.1% and 57.1% in group A, 66.1%, 83.9% and 88.7% in group B, respectively, still no significant difference existed between the two groups (x2=38.150, P value is less than 0.05; x2=12.073, P value is less than 0.05, x2=14.459, P value is less than 0.05). (3) In group A, the HBeAg seroconversion rates for male and female patients were 34.5% and 40.0% respectively, no significant difference found between. As regard ages the rates were 34.9% and 50.0% for patients younger or more than 40 years of age, no significant difference existed between. The HBeAg seroconversion rate was higher in patients with lower baseline serum HBV DNA loads ( less than 6 log10 copies/ml) . (4) The rates of patients with fever and blood abnormality were 36.7% and 34.7% in group A, 32.3% and 27.4% in group B, respectively. The total incidences of adverse events were similar between group A and B (x2=0.244, P value is more than 0.05; x2=0.682, P value is more than 0.05). (5) The ratio of drug resistance in group B was only 1.6%. The adverse events of interferon a-1b treatment for CHB are low and mild. The HBeAg seroconversion rate persistently raises with the extension of interferon a-1b treatment course. The HBV DNA undetectable rate of interferon a-1b combined with lamivudine is significantly higher than that of interferon a-1b and the drug resistance of lamivudine can be reduced obviously by combination therapy.


Han Y.,Capital Medical University
The Cochrane database of systematic reviews | Year: 2013

Postherpetic neuralgia is a common, serious painful complication of herpes zoster. Corticosteroids are anti-inflammatory and might be beneficial. This is an update of a review first published in 2008 and previously updated in 2010. To examine the efficacy of corticosteroids in preventing postherpetic neuralgia. We updated the searches for randomised controlled trials (RCTs) of corticosteroids for preventing postherpetic neuralgia in the Cochrane Neuromuscular Disease Group Specialized Register (16 April 2012), CENTRAL (2012, Issue 3), MEDLINE (January 1966 to April 2012), EMBASE (January 1980 to April 2012), LILACS (January 1982 to April 2012), and the Chinese Biomedical Retrieval System (1978 to 2012). We also reviewed the bibliographies of identified trials, contacted authors and approached pharmaceutical companies to identify additional published or unpublished data. We included all RCTs involving corticosteroids given by oral, intramuscular, or intravenous routes for people of all ages with herpes zoster of all degrees of severity within seven days after onset, compared with no treatment or placebo but not with other treatments. We did not include quasi-RCTs (trials in which a systematic method of randomisation such as alternation or hospital number was used). Two authors identified potential articles, extracted data, and independently assessed the risk of bias of each trial. Disagreement was resolved by discussion among the co-authors. Five trials were included with 787 participants in total. All were randomised, double-blind, placebo-controlled parallel-group studies. We conducted a meta-analysis of two trials (114 participants) and the results gave moderate quality evidence that oral corticosteroids did not prevent postherpetic neuralgia six months after the onset of herpes (RR 0.95, 95% CI 0.45 to 1.99). One of these trials was at high risk of bias because of incomplete outcome data, the other was at low risk of bias overall. The three other trials that fulfilled our inclusion criteria were not included in the meta-analysis because the outcomes were reported at less than one month or not in sufficient detail to add to the meta-analysis. These three trials were generally at low risk of bias. Adverse events during or within two weeks after stopping treatment were reported in all five included trials. There were no significant differences in serious or non-serious adverse events between the corticosteroid and placebo groups. There was also no significant difference between the treatment groups and placebo groups in other secondary outcome analyses and subgroup analyses. The review was first published in 2008 and no new RCTs were identified for inclusion in subsequent updates in 2010 and 2012. There is moderate quality evidence that corticosteroids given acutely during zoster infection are ineffective in preventing postherpetic neuralgia. In people with acute herpes zoster the risks of administration of corticosteroids do not appear to be greater than with placebo, based on moderate quality evidence. Corticosteroids have been recommended to relieve the zoster-associated pain in the acute phase of disease. If further research is designed to evaluate the efficacy of corticosteroids for herpes zoster, long-term follow-up should be included to observe their effect on the transition from acute pain to postherpetic neuralgia. Future trials should include measurements of function and quality of life.


Dai W.L.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2011

To study the atopy spectrum and its related factors in 1 to 3 years old children with allergic rhinitis. Ninety-six children with allergic rhinitis, aged between 1 and 3 years old, referred to ENT department of Beijing Children's Hospital between August 2009 and November 2010 were retrospectively reviewed. Data were recorded for patients' age, age stratification, sex, the age of first symptom, the duration of history, and the allergic history of children, the allergic history of parents. The screening tests on inhalant and food allergens were conducted by immunoblot assay using the Allergy Screen system. The total serum IgE level was also measured. The distribution of the inhalant and food allergens was summarized. The influence of the clinical characteristics was analyzed according to the age subgroup determined by month, allergen category and positiveness of eczema or asthma. Logistic regression was used to analyze the relationship of clinical characteristics and allergen spectrum. The total positive rates of allergic screening test rate were 81.3%. The inhalant and food allergens were 62.5% and 53.1% respectively. The commonest allergy was mixed fungal (50.0%), followed by milk (34.4%), lamb (31.3%), beef (26.0%), dust mite (21.0%), wheat (18.8%), mugwort (12.5%), egg white or egg yolk (11.5%).62.5% of patients could be diagnosed as AR, the remaining could be diagnosed temporarily as non-allergic rhinitis temporarily. Single factor analysis of clinical characteristics in different subgroup determined by month showed that: inhalant allergen (positive/negative) (χ2=13.699, P=0.001), father suffered from AR (χ2=14.060, P=0.001), and father or mother suffered from AR (χ2=7.396, P=0.025) were statistically significant at three monthly age groups. The personal history of eczema (OR=3.143, P=0.034) might increase the possibility of sensitization to allergens. The personal history of eczema (OR=3.125, P=0.015) and the total serum IgE level>200 IU/ml (OR=3.119, P=0.030) might increase the possibility of sensitization to inhalant allergens. No clinical features for food allergen sensitization was statistically significant. There was no significant difference in positive rates between inhalant and food allergens groups. The presence of inhalant allergens (OR=3.594, P=0.046), insect bites dermatitis (OR=11.941, P=0.002) were the risk factors for positiveness of eczema or asthma, and the father with AR (OR=0.251, P=0.040) as protective factors. Inhalant and food allergens all can be sensitized in the children with AR symptoms between 1 to 3 years old, and the positive rate of inhalant group is slightly higher. The differences of the inhalant allergen (positive/negative) and father suffered from AR are statistically significant at three monthly age groups. The history of eczema is the risk factor for allergen screening positive. Serum total IgE>200 IU/ml and eczema history are risk factors for inhalant allergen screening positive. The factors of inhalant allergens, insect bites dermatitis and father suffered from AR relate to any positive of eczema or asthma.


Qin N.N.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2011

Though myasthenia gravis (MG) is a typical autoimmune disorder, there was some controversy on the treatment of the childhood-onset MG. By observing the efficacy of different therapies, the authors analyzed the affecting factors of prognosis in childhood-onset MG. The retrospective data of 155 patients with childhood-onset MG (age of MG onset was less than 15 years) were collected from Department of Neurology, Beijing Tongren Hospital (January 2000 - February 2010). The patients were non-randomly divided according to their treatment into 3 groups (glucocorticoid, thymectomy and glucocorticoid combined with thymectomy groups). Postintervention status meeting the criteria of Myasthenia Gravis Foundation of America (MGFA) "complete stable remission, CSR", "pharmacologic remission, PR", "minimal manifestations, MM", or "Improved, I" was regarded as desirable response, which was used as primary indicator of observation. The authors assessed the efficacy of three therapies and analyzed the influencing factors of prognosis by using Chi-square test and Logistic regression. At 3 months of treatment, glucocorticoid group showed the highest effective rate. At the end of 1 year or 2 years of treatment, glucocorticoid combined with thymectomy group showed the highest effective rate respectively. The generalization rate of MG at 2 years, 10 years and 20 years in childhood-onset ocular MG patients were 4.3%, 10.7%, and 41.5%, respectively. Of patients with generalization of MG, 48.1% occurred within 2 years, 92.6% within 20 years. Univariate analysis showed that in childhood-onset ocular MG patients, variables such as age at onset (> 10 years), LG-MG and with chronic fatigue were significantly associated with general MG conversion. Whereas multivariate analysis showed that patients with age at onset (> 10 years) and chronic muscle fatigue were apt to convert to generalized MG. Glucocorticoid appeared to have an effect that leads to early remission of symptoms in childhood-onset MG patients and glucocorticoid combined with thymectomy appeared to have better long-term effect. For those childhood-onset ocular MG patients with longer course of disease, older age of onset, chronic fatigue, or LG-MG, physicians should try to prevent the generalization of MG by immunosuppressive therapies.


He J.X.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2011

To summarize clinical and molecular features of two children with autosomal recessive chronic granulomatous disease caused by CYBA mutations. The clinical records and CYBA mutations were reviewed for analysis of infections and inflammatory complications. The first case was a girl diagnosed with "liver and spleen abscess" in our hospital when she was 2.9 years old, with past history of neonatal impetigo and recurrent purulent lymphadenitis and positive family history. The results of DHR123 flow-cytometry showed that positive phagocytes after phorbol ester (PMA) stimulation was 84.63%. CYBA mutation analysis showed that she had heterozygous 35C > T, Q3X and IVS-2A > G. The second case was a boy diagnosed with "sepsis (salmonella D)" when he was 4 years old with a past history of impetigo, sepsis, perianal abscess, skin infection and positive family history. The results of flow cytometry showed that positive phagocytes after PMA stimulation was 96.13%. CYBA mutation analysis showed that he had homozygous 35C > T, Q3X and his parents were all carriers. All of them had BCG related axillary lymphnode calcification. A22CGD cases had recurrent purulent infections (skin, lymphnode, liver and spleen, lung, blood), DHR123 flow cytometric analysis helped the diagnosis of CGD, CYBA mutation analysis ascertained the diagnosis of A22CGD.


Meng W.L.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To investigate the prevalence of impaired glucose-insulin metabolism in obstructive sleep apnea hypopnea syndrome (OSAHS); to examine the relation between severity of OSAHS and impaired glucose metabolism; and to evaluate the effectiveness of continuous positive airway pressure (CPAP) on impaired glucose metabolism. A total of 214 patients who were free of diabetes at baseline underwent both nocturnal polysomnography (PSG), and 2-h oral glucose-tolerance test, insulin and hemoglobin A1c test. CPAP treatment for glucose-insulin metabolism (+) was given to OSAHS group after informed consent had been obtained. Eighty-eight patients and 17 patients with impaired glucose-insulin metabolism were found in OSAHS group and the control group respectively. Impaired glucose-insulin metabolism was present in 54.3% of OSAHS group and 32.7% of control group. Logistic regression analysis showed a significant positive correlation with OSAHS (AHI ≥ 10 times/h) and impaired glucose-insulin metabolism in all patients (OR = 2.440, 95%CI 1.201 - 4.958). Plasma glucose level changes had no significant differences between before and after CPAP treatment (P > 0.05). OSAHS is associated with a high frequency of impaired glucose metabolism. The relationship between OSAHS and impaired glucose metabolism is independent of obesity. Longest apnea time (LAT) and AHI are important contributors to impaired glucose metabolism in OSAHS patients. Short-term CPAP therapy has no significant improvement on glucose metabolism in patients with OSAHS.


Zhang B.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2011

To investigate the proportion of children with congenital abnormalities of the kidney and urinary tract (CAKUT) among those who received screening, analyze the rate, diagnosis and treatment of each abnormality, and evaluate the value of ultrasonography in the CAKUT diagnosis. Totally 489 of 26 989 children who received screening for urinary tract abnormalities were found to have CAKUT and were followed up by telephone interviews and were asked whether they had defined diagnosis of CAKUT, had clinical symptoms and received treatment. Children who had been operated on were also asked about the operation time, surgical procedure, postoperative diagnosis, and outcome. Children who had never been reviewed or still had no diagnosis were arranged to Beijing Children's Hospital for ultrasound follow-up. The final data were summed up for a retrospective analysis. 1) The proportion of children with CAKUT was 1.67%. Most children with CAKUT had no clinical manifestations. 2) Of the 489 cases, 320 were followed up, and 169 were lost. Of the cases who were followed up, 16 out of 295 cases who had clear diagnosis showed inconsistent results in ultrasound screening. 3) Hydronephrosis was identified in 137 cases (0.78%), among whom 111 were males and 26 were females, in 98 cases the hydronephrosis was on the left, in 28 on the right and in 11 were bilateral. Ureterovesical junction obstruction occurred in 33 cases (0.19%), 25 males and 8 females, in 22 cases on the left, in 10 on right and bilateral in 1 case. Renal dysplasia was shown in 30 cases (0.17%), 22 males and 8 females; renal cysts in 26 cases (0.15%); duplex kidney in 24 cases (0.14%); solitary kidney in 22 cases (0.12%); ureterocele in 16 cases (0.09%); fused kidney in 10 cases (0.06%); renal hypoplasia in 9 cases (0.05%); ectopic kidney in 5 cases (0.03%); polycystic kidney in 3 cases (0.02%). Primary vesicoureteral reflux, posterior urethral valves, renal malrotation, branch renal pelvis, extrarenal pelvis in 1 case each. 4) Totally 114 of the 295 cases (38.6%) who were followed up for CAKUT in screening test required surgical treatment. Only one case of ureteropelvic junction obstruction (UPJO) failed in surgery. The proportion of children with CAKUT was high. CAKUT is difficult to detect since there is no significant clinical manifestations in early ages. More than one third of patients with CAKUT needed surgical treatment. Ultrasonography showed obvious advantages in detecting children's CAKUT, proven to be the most preferred method of screening, thus could be recommended as a routine of children's physical examination.


To investigate the relationship between inflammatory factors, including C-reactive protein(CRP), tumor necrosis factor alpha (TNF-α), adiponectin, leptin and gestational diabetes mellitus (GDM) and their changes in puerperium. From June 2008 to May 2010, 40 cases with gestational diabetes mellitus, 40 cases of normal pregnancy were enrolled in this study. Fasting venous blood were obtained at early pregnancy (10 - 12 weeks), late pregnancy (36 - 38 weeks) and day 3 and 42 of postpartum. Serumal adiponectin, leptin and TNF-α were measured by ELISA, and serumal CRP were measured by particle enhanced immunoturbidimetric method. The levels of those 4 cytokines and homeostasis model assessment insulin resistance (HOMA-IR) index were compared between two groups. And the changes of 4 cytokines were calculated in puerperium. The receiver operating characteristic (ROC) for TNF-α predicting GDM was designed. (1) The relationship between cytokines and HOMA-IR: the levels of adiponectin of (5.7 ± 1.8) mg/L in the GDM group were significantly lower than (8.1 ± 2.7) mg/L in control group in early pregnancy, and the level of adiponectin was negatively correlated with HOMA-IR (r = -0.333, P < 0.05). The levels of (28 ± 10) μg/L of leptin, (10.0 ± 3.4) ng/L of TNF-α and (4.7 ± 1.1) mg/L of CRP in GDM group were significantly higher than (20 ± 8) μg/L of leptin, (4.6 ± 2.7) ng/L of TNF-α, (2.4 ± 1.2) mg/L of CRP in control group, which were positively correlated with HOMA-IR (r = 0.411, 0.529, 0.308, all P < 0.05). In late pregnancy, the level of adiponectin (3.9 ± 2.2) mg/L in the GDM group was significantly lower than (6.6 ± 2.7) mg/L in control group, and the level of adiponectin was negatively correlated with HOMA-IR (r = -0.344, P < 0.05). The levels of (37 ± 13) μg/L of leptin, (12.7 ± 2.6) ng/L of TNF-α and (6.7 ± 3.6) mg/L of CRP in the GDM group were significantly higher than (30 ± 13) μg/L of leptin, (5.8 ± 2.1) ng/L of TNF-α, (4.4 ± 3.1) mg/L of CRP in control group, which were positively correlated with HOMA-IR (r = 0.414, 0.487, 0.285, all P < 0.05). Multiple linear regression analysis showed that the level of TNF-α at early and late pregnancy was most correlated with HOMA-IR (r = 0.390, 0.284, all P < 0.05). (2) The level of the cytokines and HOMA-IR in puerperium: at day 3 of postpartum, the level of adiponectin of (3.3 ± 1.1) mg/L in the GDM group was significantly lower than (6.2 ± 1.5) mg/L in control group, which was negatively correlated with HOMA-IR (r = -0.283, P < 0.05). The levels of (31 ± 13) μg/L of leptin, (10.1 ± 5.7) ng/L of TNF-α and (35.1 ± 6.5) mg/L of CRP in the GDM group were significantly higher than (21 ± 15) μg/L of leptin, (5.6 ± 3.0) ng/L of TNF-α, (30.5 ± 8.5) mg/L of CRP in control group. And leptin and TNF-α levels were positively correlated with HOMA-IR (r = 0.372, 0.494, all P < 0.05). At day 42 of postpartum, the level of adiponectin in GDM group was negatively correlated with HOMA-IR (r = -0.299, P < 0.05), and the levels of leptin and TNF-α were positively correlated with HOMA-IR (r = 0.401, 0.442, all P < 0.05). Multiple linear regression analysis showed that the level of TNF-α at day 3 and day 42 was most correlated with HOMA-IR (r = 0.363, 0.274, all P < 0.05). (3) ROC analysis of data from early pregnancy showed that the threshold for TNF-α to predict GDM was 5.45 ng/L. TNF-α might be the index to predict GDM and evaluate prognosis.


Xie Z.D.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2011

Viruses are common pathogens of acute lower respiratory tract infection (ALRTI) in children. There are few studies on consecutive monitoring of viral pathogens of ALRTI in a larger cohort during the past several years. The aim of this study was to investigate the viral pathogens of ALRTI in children of different age groups and to outline the epidemic feature of different viruses. (1) Totally 1914 (1281 male and 709 female) children with clinical diagnosis of ALRTI during the period of March 2007 to March 2010 were recruited into this study. These patients were hospitalized patients in department of internal medicine or outpatients in emergency department in Beijing Children's Hospital. The patients were divided into four groups, including 1072 patients < 1 year old, 326 patients 1- < 3 years old, 158 patients 3- < 6 years old, 358 patients ≥ 6 years old. One nasopharyngeal aspirate specimen was collected from each patient. Reverse transcription (RT) PCR methods were applied to detect common respiratory viruses including respiratory syncytial virus (RSV), human rhinovirus (HRV), influenza virus type A, B and C (IFA, IFB, IFC), parainfluenza virus (PIV) type 1-4, adenovirus (ADV), enterovirus (EV), human coronavirus (HCOV), human metapneumovirus (HMPV) and human bocavirus (HBOV). (1) The total positive rate of viruses was 70.3%. The positive rate was 83.0% (890/1072) in the group of < 1 year old, and 80.1% (261/326) in group of 1- < 3 years old, 60.8% (96/158) in group of 3- < 6 years old and 27.7% (99/358) in group of ≥ 6 years old, respectively. There was a significant difference in the positive rate among different age groups (χ 2 = 2213.5, P = 0.000). The top three viruses were RSV, HRV and PIV; and the positive rates were 50.9%, 36.2% and 12.0% respectively in group of < 1 year old. (2) The epidemic seasons of RSV and HRV were winter and spring, and PIV infection was epidemic in spring and summer. (3) The detection rates of 2 or more viruses were 38.2%, 36.4%, 30.2% and 15.2% in groups of < 1 year old, 1- < 3 years old, 3- < 6 years old and ≥ 6 years old, respectively. There was a significant difference in the mixed infection rate among different age groups (χ 2 = 1346.00, P = 0.000). RSV, HRV and PIV were the most predominant pathogens in younger children with ALRTI. Different viral infections had different seasonal features. Mixed infections with two or more viruses were detected in substantial proportion of patients with ALRTI, but further studies are needed to explore the clinical significance of mixed infection with viruses in patients with ALRTI.


Du X.M.,Capital Medical University
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology | Year: 2012

To investigate the expression and clinical significance of calcium-binding protein S100A9 in the patients of breast cancer. The serum S100A9 level from 39 cases of preoperative breast cancer, 15 postoperative patients, and 10 healthy women was detected by ELISA. Immunohistochemistry was used to detect the S100A9 expression in 12 specimens of breast cancer tissues. The serum S100A9 level was significantly higher in the preoperative breast cancer patients than that in the healthy women (P<0.01) and the postoperative breast cancer patients(P<0.01). The serum S100A9 level of the breast cancer patients with lymph node metastasis was significantly higher than those without lymph node metastasis (P<0.01). S100A9 level in the serum was related to patient age, tumour size, histological grade, and pathological type (P<0.01). The expression of S100A9 protein in breast cancer tissues was significantly higher than that in paracancerous benign breast tissues. The expression of S100A9 significantly increases in the serum and tissues of patients with breast cancer, which indicates that S100A9 may function as a marker of diagnosis and treatment effect in breast cancer.


Chang P.Y.,Capital Medical University
Genetics and molecular research : GMR | Year: 2011

We investigated a possible association between genetic variations in the thiazide-sensitive Na-Cl cotransporter (TSC) gene and essential hypertension (EH) in the Mongolian and Han ethnic groups in Inner Mongolia. Our study included 385 unrelated Mongolian herdsmen and 523 Han farmers. Nine tagSNPs of TSC were identified from the Chinese HapMap database based on pairwise r(2) ≥ 0.5 and minor allele frequency ≥0.05. Genotyping was performed using the PCR/ligase detection reaction assay. Association between tagSNPs and hypertension was investigated under the additive model. There were significant differences between the genotype and allele frequencies of rs13306673 between the EH group and the control group in the Han population. Significant associations were found between the rs7204044 variant and EH in both the Mongolian and Han ethnic groups. The frequency of haplotype GCA in the EH group was significantly higher than in the control group in the Mongolian population. In the Han population, the frequency of haplotype TGG was significantly higher in the EH group than in controls, whereas haplotype TGA occurred significantly less often in EH than in controls. We suggest that rs7204044 of TSC is a genetic factor for EH in these two ethnicities and that rs13306673 is a genetic factor for EH in the Han population.


Wang C.,Beijing Hospital | Wang C.,The People's Association | Rao K.,Beijing Hospital | Wu S.,Beijing Hospital | Liu Q.,Capital Medical University
Chest | Year: 2013

Over the past 2 decades, signifi cant progress has been made in improving the health-care system and people's health conditions in China. Following rapid economic growth and social development, China's health-care system is facing new challenges, such as increased health-care demands and expenditure, ineffi cient use of health-care resources, unsatisfying implementation of disease management guidelines, and inadequate health-care insurance. Facing these challenges, the Chinese government carried out a national health-care reform in 2009. A series of policies were developed and implemented to improve the health-care insurance system, the medical care system, the public health service system, the pharmaceutical supply system, and the health-care institution management system in China. Although these measures have shown promising results, further efforts are needed to achieve the ultimate goal of providing affordable and high-quality care for both urban and rural residents in China. This article not only covers the improvement, challenges, and reform of health care in general in China, but also highlights the status of respiratory medicine-related issues. CHEST 2013; 143(2):524-531 © 2013 American College of Chest Physicians.


Yang J.,Capital Medical University
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2013

To investigate the expression of NPM- ALK fusion gene in bone marrow (BM) and peripheral blood (PB) in anaplastic large cell lymphoma (ALCL) patients and its prognostic significance. NPM- ALK fusion gene of 21 BM and 15 PB samples from patients with NPM-ALK positive ALCL was detected by RT- PCR, and the relationship between NPM- ALK expression and prognosis and clinical characters was evaluated. Of the 21 patients, 12 cases were male and 9 case were female with a median age of 9 (range, 2-14) years old. The median follow- up was 31 months. Patients with a positive NPM-ALK expression in BM had a 3-years EFS of (35.6±18.6)%, compared with (91.7±8.0)% for patients with negative NPM-ALK (P=0.038). The incidence of positive expression in BM was significantly higher in patients who had more than 3 organs involved by tumor (P=0.032). 86.7% patients had a concordant results of NPM-ALK expression in PB and BM. We could evaluate the minimal disseminated disease of NPM-ALK positive ALCL patients by screening the NPM-ALK fusion gene in BM and PB by RT-PCR. The positive expression is associated with a poor prognosis and could be used for stratification of ALCL.


Recurrent stroke following a transient ischemic attack or minor stroke is frequent but potentially preventable. The prevention outcome is, by and large, dependent on how well physicians understand and implement prevention guidelines. Yet, little is known about the scope of physicians' awareness and implementation of secondary stroke prevention guidelines in the mainland of China. This study aimed to identify the gaps between physicians' clinical practice and guidelines for secondary stroke prevention in China. A nation-wide online survey with a 16-item questionnaire that reflects major components of the Chinese National Secondary Stroke Prevention Guidelines was performed between September 1, 2009 and October 31, 2009. A total of 3489 neurologists from third tier hospitals completed the survey. The percentage of the physicians who were aware of the control targets and prevention measures recommended in the Chinese national guidelines was generally lower than reported for developed countries. Physicians in the mainland of China lack sufficient awareness of secondary stroke prevention guidelines. Continuing education is highly recommended.


Yao X.,Capital Medical University
Cancer Research and Clinic | Year: 2013

Objective: To study the characteristics of rat model of bone metastasis from breast cancer, which receiving intra-tibial injection of MRMT-1 rat breast cancer cells. Methods: Female SD rats were divided randomly into sham operation group and model group. Sham operation group were intra-tibial injected normal saline, while model group were injected the MRMT-1 cells to develop model. On day 19 after operation, allodynia and hyperalgesia were tested. All rats were killed on day 21, tibias were collected to measure the volume of tumors, determinate bone mineral density (BMD) and bone mineral capacity (BMC). Through HE staining, tartrate-resistant acid phosphatase (TRAP) staining and immunohistochemistry staining to observe the bone pathological changes, count osteoclasts and semi-quantitatively measure expressions of proliferating cell nuclear antigen (PCNA), osteoprotegerin (OPG) and receptor activator of nuclear factor-κB ligand (RANKL). By real-time quantitative RT-PCR, expression of parathyroid hormone related protein (PTHrP) was determinated. Results: Model group displayed mechanical allodynia and hyperalgesia (P < 0.01), bone destruction was found by image examination (P < 0.01). BMD of left tibia decreased to (0.11±0.01) g/cm2 from (0.13±0.02) g/cm2. In the model tibia, visible tumor growth could be seen, and mixed bone lesion was seen through HE staining sections. Osteoclast enhanced from (1.88±2.92)/HP to (40.84±25.59)/HP (P < 0.01), PTHrP and OPG reduced (P < 0.05), but RANKL did not change. Conclusion: The model of bone metastasis from breast cancer show characteristics of cancer pain and bone lesion. The injury mechanism is to break balance of OPG-RANKL-RANK system by inhibiting OPG, these changes would cause excessive activation of osteoclasts, which induced hyperfunction of bone resorption.


Wang C.,Capital Medical University
Neurobiology of aging | Year: 2013

Genome-wide association and large-scale replication studies have linked Parkinson's disease (PD) to a locus on 4p15 encompassing a single gene encoding bone marrow stromal cell antigen 1 (BST1). To screen for causative mutations of BST1 in PD, we have directly sequenced all the 9 exons of BST1 in a Chinese cohort consisting of 524 PD cases and 527 controls. As a result, 6 known and 1 novel single-nucleotide polymorphisms (SNPs) were identified in exons 1, 3, 4, 7, and 9. However, none of these SNPs were associated with PD. The data, together with previous reports, suggested that the association between BST1 and PD might be determined by the noncoding sequences of the gene. Copyright © 2013 Elsevier Inc. All rights reserved.


Li M.,Capital Medical University | Lincoln P.,Chalmers University of Technology | Andersson J.,Chalmers University of Technology
Journal of Physical Chemistry B | Year: 2011

Threading intercalation is an unusual DNA binding mode that displays extremely slow dissociation kinetics, which is an important feature for cytotoxicity, making threading intercalating compounds interesting as model compounds in the search for new DNA binding drugs. This type of binding has for ruthenium complexes previously only been observed for complexes containing 11-substituted dipyridophenazine ligands. In this work we have synthesized and investigated the DNA binding properties of two new 10,13-diarylsubstituted dipyridophenazine ruthenium complexes, using spectroscopic techniques, and found that this substitution pattern provides a new strategy for development of drugs with slow dissociation kinetics. However, the nature of the aryl substituents largely affects the binding properties of the complexes as it was found that a dithienyl substituted complex exhibit slow dissociation kinetics characteristic for threading intercalation while its diphenyl substituted analogue seems to bind DNA by partial intercalation of one phenyl substituent resulting in faster dissociation. © 2011 American Chemical Society.


Li G.,Capital Medical University
Cancer Research and Clinic | Year: 2016

Objective: To evaluate the expression of immunohistochemistry markers p53, p16 and Ki-67 in penile cancer and penile intraepithelial neoplasia (PIN). Methods: 33 patients who suffered penile cancer and received organ-sparing surgery from Jan 2004 to Dec 2013 were retrospectively analysed. The expression of p53, p16 and Ki-67 were detected by EnVision/HRP two steps immunohistochemistry. Results: The positive expression rates of p53 in normal penile epithelium, low-grate penile intraepithelial neoplasia, high-grate penile intraepithelial neoplasia, well-differentiated squamous cell carcinoma and poor-differentiated squamous cell carcinoma were 0, 16.7 %, 38.5 %, 30.0 % and 66.7 % respectively. The expression rates of p16 were 20.0 %, 50.0 %, 53.8 %, 70.0 % and 33.3 % respectively. The expression rates of Ki-67 were 0, 16.7 %, 61.5 %, 65.0 % and 75.0 % respectively. Conclusions: The combined detection of p16 and Ki-67 can contribute to discover PIN and penile carcinoma much more earlier. p53 may be related to the occurrence and development of low differentiated squamous cell carcinoma, and p16 may be related to the occurrence and development of well-differentiated squamous cell carcinoma.


Li H.M.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2010

To explore the clinical manifestation, immune abnormality and outcome of disseminated Bacille Calmette-Guérin (BCG) infection in children. The clinical data of 18 children with disseminated BCG infection seen from January 2000 to December 2007 were analyzed retrospectively. Thirteen of the children were male among 18 patients. Disseminated infection first appeared in armpit lymph nodes ipsilateral to the vaccination site, then spread to lungs in 15, lymphnodes of mediastinum or abdominal cavity in 18, skin and soft tissues in 5, skeletons in 4, liver in 4, spleen in 8, kidney, adrenal gland or meninges in 3. Twelve children were diagnosed to have primary immunodeficiency; 3 had severe combined immunodeficiency (SCID); 7 had chronic granulomatous disease (CGD), 2 had IL-12/IFN-gamma passageway deficiency. Eleven of the 18 patients died, and the remaining 7 patients were followed up from 1 to 9 years and are alive at present, but presented recurrent skin and bone tuberculosis in 4 and recurrent other infection in 3. Most Children with disseminated BCG infection had primary immunodeficiency. CGD and IL-12/IFN-gamma passageway deficiency accounted for considerable proportion, so special immune function should be detected in these patients. The prognosis was poor. The type of the immunodeficiency diseases should be identified in early stage and the specific immune treatment should be given to the patients.


Sun Z.,Curtin University Australia | Xu L.,Capital Medical University
BioMed Research International | Year: 2014

Coronary computed tomography angiography (CCTA) has been recently evaluated for its ability to assess coronary plaque characteristics, including plaque composition. Identification of the relationship between plaque composition by CCTA and patient clinical presentations may provide insight into the pathophysiology of coronary artery plaque, thus assisting identification of vulnerable plaques which are associated with the development of acute coronary syndrome. CCTA-generated 3D visualizations allow evaluation of both coronary lesions and lumen changes, which are considered to enhance the diagnostic performance of CCTA. The purpose of this review is to discuss the recent developments that have occurred in the field of CCTA with regard to its diagnostic accuracy in the quantitative assessment of coronary plaques, with a focus on the characterization of plaque components and identification of vulnerable plaques. © 2014 Zhonghua Sun and Lei Xu.


Tomson T.,Karolinska Institutet | Xue H.,Capital Medical University | Battino D.,Irccs Neurological Institute Carlo Besta Foundation
Seizure | Year: 2015

It has been long known that the risk of major congenital malformations is increased among children of mothers with epilepsy. This is mainly due to the teratogenic effects of antiepileptic drugs although other factors, such as genetically determined individual susceptibility, are likely to contribute. Recent large scale prospective epilepsy and pregnancy registries have indicated that the rate of major congenital malformations may be at most two-fold higher than expected with exposure in utero to the presently most frequently used antiepileptic drugs such as carbamazepine or lamotrigine. Higher rates are consistently reported with exposure to valproate. The risk of teratogenic effects appears to be dose dependent and the lowest effective dose should thus be established before pregnancy regardless of which antiepileptic drug the woman is taking. Major changes such as switches between drugs should be avoided when pregnancy is established.


Zhang L.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2010

To investigate the understanding among otolaryngologists and perspectives on the diagnosis and management of allergic rhinitis in major cities in China. A survey among otolaryngologists in mainland China was carried out through questionnaire, including the medical history, diagnosis and pharmacotherapy. The survey was conducted in 508 otolaryngologists. In general otolaryngology head and neck surgery clinic, the patients with allergic rhinitis was about 19% +/- 14%, which was of 35% +/- 17% in rhinological clinic. Most of the otolaryngologists (61% +/- 29%) diagnosed AR based on medical history and nasal examination. Only 35% +/- 28% of the otolaryngologists used skin prick test or specific IgE serum test. 70% +/- 27% of the patients with AR were offered nasal steroids, 49% +/- 32% and 36% +/- 28% of the patients were offered oral or nasal antihistamines respectively. Only 24% +/- 17% of patients realized the importance of follow-up. Allergic rhinitis is a core component in the specialty of otolaryngology head and neck surgery. The diagnosis and management of allergic rhinitis is sub-optimal. Chinese otolaryngologists need continuous education in aspects of diagnosis and management of allergic rhinitis.


Ma Y.Y.,Capital Medical University
Zhonghua yi xue za zhi | Year: 2010

OBJECTIVE: To analyze the characters of bronchial foreign bodies in children and the utilization of bronchoscope in the treatment of bronchial foreign bodies. METHODS: A total of 246 children were diagnosed with bronchial foreign bodies at our hospital during January 2000 until August 2009. Under local mucosal anesthesia, a bronchoscope was inserted through nasal cavity into bronchi. After identifying the site of foreign body, grasping forceps was guided through bronchoscope to remove the foreign body from airway. RESULTS: Among 246 cases, hard nut and skin of melon seed were found (n = 230, 93.5%). The most common site of foreign body was in right lower lobe bronchi (n = 98, 38.9%). The average operative frequency was 1.9 +/- 1.3 and one-time extraction ratio 58.5% (n = 144). The one-time extraction ratio of patients with foreign body obstructed in main bronchi (91.1%), right middle lobe (60.0%) and right lower lobe (55.1%) was higher than others. The operation frequency of using basket grasping forceps (1.4 +/- 0.9) was lower than those using tooth type forceps (2.1 +/- 1.4). And the difference was significant (P = 0.000). CONCLUSION: For bronchial foreign body in pediatric patients, hard nut and skin of melon seed are the most common foreign bodies. The right and left lower lobe bronchi are the predilection site. Foreign body in main bronchus is the easiest to be removed by grasping forceps. For massive foreign bodies, basket grasping forceps fares better than tooth grasping forceps.


Wang T.,Capital Medical University
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2010

To observe the influence of paclitaxel on the proliferation of human pulmonary fibroblast (HPF), and therefore to explore the possible effect on granulation tissue formation in-stent restenosis after airway stent. The HPFS were divided into 3 groups: 24 h group, 48 h group and 72 h group, and there were 3 different dosage groups in each time group. MTT assay was used for detecting cell proliferation. The inhibition ratio of 4.5 microg/ml group, 13.5 microg/ml group and 45 microg/ml group were 43.7%, 58.8% and 68.1% after 24 hours; 75.5%, 77.7%, 91.9% after 48 hours; 95.1%, 97.1%, 97.0% after 72 hours. Paclitaxel inhibited the proliferation of HPF, and the inhibition ratio has partly relation with time and dosage.


Liao L.,Capital Medical University | Schaefer W.,University of Pittsburgh
International Urology and Nephrology | Year: 2014

Objective: To establish typical value ranges (TVRs) and to outline their role in urodynamic quality control. Methods: Five hundred and eighty-two data sets of free flow, filling cystometry, and voiding from 181 males in a strictly quality-controlled study were analyzed for intravesical pressure (P ves), abdominal pressure (P abd), and detrusor pressure (P det) before, at the beginning and end of filling, and after voiding. Cystometric capacity, maximum flow rate (Q max), voided volume (V void), and compliance were determined. Mean value, standard deviation, median, and various TVRs were calculated. Technical errors related to the TVRs are classified and examples given. Results: TVRs for initial resting P ves, P abd, and P det were 31-42, 28-39, and 0-4 cmH2O, respectively. Various technical errors are classified as type I: normal initial P det, both P ves and P abd are incorrect; type II: negative initial P det; and type III: initial P det too high. The incidences of I, II, and III errors were 9.8, 4.5, and 1.4 %, respectively. The TVRs for maximum cystometric capacity and compliance were 157-345 mL and 26.7-70.8 mL/cmH2O; Q max, P det, and V void were 5.5-9 mL/s, 57-92 cmH2O, and 167-315 mL, respectively; Q max and V void in free flow were 8-9.2 mL/s and 167-301 mL, respectively. After voiding, two errors were found: type V (P ves and P det after voiding still high) and type VI (P ves and P det negative). Conclusions: TVRs in urodynamics are indispensable and effective tools for quantitative plausibility checks and quality control. They are sensitive and reliable indicators for correct measurement and a relevant contribution to a collection of normal values. © 2014 Springer Science+Business Media.


To evaluate the efficacy and safety of Chinese medicinal shensongyangxin capsules in the treatment of paroxysmal atrial fibrillation. From August 2007 to July 2008, Beijing Chaoyang Hospital conducted a multicenter study, select the eleven hospital's outpatient subjects, aged 18 to 75 years old, male or female, paroxysmal atrial fibrillation (at least one electrocardiogram diagnosis) seizure frequency ≥ 2 times/month, according to the ratio 1:1:1, subjects were randomly divided into three groups: a. shensongyangxin group, taking shensongyangxin capsule 4 + propafenone analogues 150 mg, 3 times a day; b. propafenone group, taking propafenone tablets 150 mg + 4 shensongyangxin analogues, 3 times a day; shensongyangxin capsule + propafenone group, taking shensongyangxin capsule 4 + propafenone 150 mg, 3 times a day. The treatment course is 8 weeks, with 3 times of follow-up. Total of 349 cases of paroxysmal atrial fibrillation, which 117 cases in shensongyangxin group, 115 cases in propafenone group; 117 cases in shensongyangxin + propafenone group. The baseline data analysis showed that there were no significantly difference (P > 0.05) among the three groups of atrial fibrillation seizure frequency, vital signs, general condition, medical history, 24-hour ambulatory ECG, 12-lead normal electrocardiogram, cardiac ultrasound and symptoms. The comparison before and after (8 weeks) treatment showed that the frequency (from 6 times/m to 2 times/m in each group, P < 0.01), number of cases [from 46 (43.3%) to 22 (20.8%), 43 (43.4%) to 25 (25.3%), and 40 (40.6%) to 31 (29.2%), respectively P < 0.01] and duration time of attack of atrial fibrillation (from 4 h to 0.5 h, 4 h to 0.5 h, and 4.25 h to 0.5 h, respectively P < 0.01) all decreased in three groups. No significant difference among the three groups comparing the overall effect (62.3%, 58.6%, and 58.5%, respectively, P > 0.05), while the efficacy of TCM symptoms in shensongyangxin group (80.2%) was better than that of propafenone group (67.7%) (P < 0.05). Safety evaluation showed that adverse reaction rate was 1.8% in shensongyangxin group, and 8.2% and 5.4% in propafenone group and shensongyangxin + propafenone group. Shensongyangxin capsules and propafenone have comparable efficacies in the treatment of PAF. The efficacy of TCM symptoms is better than propafenone. Shensongyangxin capsules have an excellent profile of safety.


Zhang D.,Chongqing Medical University | Wang Y.,National Center for Tuberculosis Control and Prevention | Lu J.,Capital Medical University | Pang Y.,National Center for Tuberculosis Control and Prevention
Antimicrobial Agents and Chemotherapy | Year: 2016

The combination of β-lactams and β-lactamase inhibitors has been shown to have potent in vitro activity against multidrug-resistant tuberculosis (MDR-TB) isolates. In order to identify the most potent β-lactam-β-lactamase inhibitor combination against MDR-TB, we selected nine β-lactams and three β-lactamase inhibitors, which belong to different subgroups. A total of 121 MDR-TB strains were included in this study. Out of the β-lactams used herein, biapenem was the most effective against MDR-TB and had an MIC50 value of 8 μg/ml. However, after the addition of clavulanate or sulbactam, meropenem exhibited the most potent anti-MDR-TB activity with an MIC50 value of 4 μg/ml. For meropenem, 76 (62.8%), 41 (33.9%), and 22 (18.2%) of the 121 MDR-TB strains were subjected to a synergistic effect when the drug was combined with sulbactam, tazobactam, or clavulanate, respectively. Further statistical analysis revealed that significantly more strains experienced a synergistic effect when exposed to the combination of meropenem with sulbactam than when exposed to meropenem in combination with tazobactam or clavulanate, respectively (P < 0.01). In addition, a total of 10.7% (13/121) of isolates harbored mutations in the blaC gene, with two different nucleotide substitutions: AGT333AGG and ATC786ATT. For the strains with a Ser111Arg substitution in BlaC, a better synergistic effect was observed in the meropenem-clavulanate and in the amoxicillin-clavulanate combinations than that in a synonymous single nucleotide polymorphism (SNP) group. In conclusion, our findings demonstrate that the combination of meropenem and sulbactam shows the most potent activity against MDR-TB isolates. In addition, the Ser111Arg substitution of BlaC may be associated with an increased susceptibility of MDR-TB isolates to meropenem and amoxicillin in the presence of clavulanate. Copyright © 2015, American Society for Microbiology. All Rights Reserved.


Wang Y.H.,Capital Medical University
Zhonghua yi xue za zhi | Year: 2011

To explore the correlations of serum alanine aminotransferase (ALT), insulin resistance and pancreatic B-cell function. A total of 351 first-degree relatives of type 2 diabetes mellitus received a standard oral glucose tolerance test (OGTT) at our outpatient clinic. All subjects were analyzed for the parameters of body mass index (BMI), waist-hip ratio, blood pressure (BP), serum lipids, ALT, aspartate aminotransferase (AST), plasma glucose (PG), true-insulin and proinsulin. Homeostasis model assessment (HOMA) was applied to assess the status of insulin resistance and pancreatic B-cell function. They were divided into 4 groups according to the quartiles of ALT: ALT1 group (< 12.9 U/L), ALT2 group (12.9 - 17.3 U/L), ALT3 group (17.4 - 24.2 U/L) and ALT4 group (≥ 24.2 U/L). The diagnosis of metabolic syndrome was made according to the definition of Chinese Diabetic Society. With the rising serum ALT levels (ALT4 vs ALT1), the levels of BMI [(26.3 ± 2.9) kg/m(2) vs (23.2 ± 3.7) kg/m(2), P < 0.01], HOMA-IR [1.93 (1.21 - 3.26) vs 1.06 (0.65 - 1.54), P < 0.01] and LnHOMA-beta (2.00 ± 0.32 vs 1.87 ± 0.28, P < 0.05) were elevated; BP, serum lipids, PG, true-insulin and proinsulin also increased (P < 0.05 or P < 0.01). The levels of serum ALT [23.3 (16.3 - 37.6) vs 14.3 (10.3 - 18.5) U/L, P < 0.01] and AST [21.5 (18.3 - 32.8) U/L vs 17.9 (15.5 - 22.1) U/L, P < 0.01] increased with the rising number of metabolic disorders (0 vs 3 - 4 metabolic disorders). After adjustments for gender, age, BMI and waist-hip ratio, serum ALT were still positively correlated with BP, serum lipids, PG, fasting true-insulin, 2 h proinsulin, 2 h proinsulin/true-insulin, HOMA-IR and the numbers of metabolic disorder (r = 0.117 - 0.236, P < 0.05 or P < 0.01). After adjustments for gender, age, BMI, waist-hip ratio and HOMA-IR, the serum ALT level remained positively correlated with the numbers of metabolic disorders (r = 0.120, P < 0.05). Multiple stepwise regression analysis showed that triglyceride, HOMA-IR, total cholesterol and 2 h-proinsulin were the independent risk factors for the level of serum ALT (P < 0.05 or P < 0.01). The elevated levels of serum ALT are significantly correlated with metabolic syndrome, insulin resistance and compensatory increases of pancreatic B-cell function. Independently of insulin resistance, the serum ALT level is correlated with metabolic syndrome.


Wang J.,Capital Medical University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2011

To study whether programmed cell death 4 (PDCD4) is involved in the development and progression of laryngeal squamous cell carcinomas (LSCC). The expressions of PDCD4 protein were examined by immunohistochemical staining and Western blot in LSCC tissues and their surrounding laryngeal normal mucosa tissues. The positive rate of PDCD4 protein in LSCC was significantly lower than that in the laryngeal normal mucosa tissues by immunohistochemical staining (P<0.01). No correlation was found between positive expression of PDCD4 and gender, age, tumor site, TNM stage, but there was significant correlation between positive expression of PDCD4 from differentiation grading and lymphatic matastasis. Meanwhile,we got the similar results by Western blot (P<0.01). The low expression of PDCD4 protein is closely related to the development and progression of LSCC. PDCD4 might be a novel molecular marker for prognosticating metastases and prognosis of LSCC.


Zhang Y.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2012

To investigate the genetic association pattern between single-nucleotide polymorphisms (SNP) in the interleukin-1 receptor-associated kinase 4 (IRAK-4) gene and allergic rhinitis (AR). A population of 379 patients with the diagnosis of AR and 333 healthy controls who lived in Beijing region was recruited. A total of 8 reprehensive marker SNP which were in IRAK-4 gene region were selected according to the Beijing people database from Hapmap website. The individual genotyping was performed by MassARRAY platform. SPSS 13.0 software was used for statistic analysis. Subgroup analysis for the presence of different allergen sensitivities displayed associations only in the house dust mite-allergic cohorts (rs3794262: P = 0.0034, OR = 1.7388; rs4251481: P = 0.0023, OR = 2.6593), but not in subjects who were allergic to pollens as well as mix allergens. The potential genetic contribution of the IRAK-4 gene to AR demonstrated an allergen-dependant association pattern in Chinese population.


Sun H.-R.,Lanzhou University | Lu X.,Capital Medical University | Ruan S.,University of Miami
Mathematical Biosciences | Year: 2010

This paper is concerned with the qualitative analysis of two models [S. Bonhoeffer, M. Lipsitch, B.R. Levin, Evaluating treatment protocols to prevent antibiotic resistance, Proc. Natl. Acad. Sci. USA 94 (1997) 12106] for different treatment protocols to prevent antibiotic resistance. Detailed qualitative analysis about the local or global stability of the equilibria of both models is carried out in term of the basic reproduction number R0. For the model with a single antibiotic therapy, we show that if R0<1, then the disease-free equilibrium is globally asymptotically stable; if R0>1, then the disease-endemic equilibrium is globally asymptotically stable. For the model with multiple antibiotic therapies, stabilities of various equilibria are analyzed and combining treatment is shown better than cycling treatment. Numerical simulations are performed to show that the dynamical properties depend intimately upon the parameters. © 2010 Elsevier Inc.


Shi F.-D.,Tianjin Medical University | Shi F.-D.,Barrow Neurological Institute | Jia J.-P.,Capital Medical University
Neurology | Year: 2011

In the wake of dramatic economic success during the past 2 decades, the specialized field of neurology has undergone a significant transformation in China. With an increase in life expectancy, the problems of aging and cognition have grown. Lifestyle alterations have been associated with an epidemiologic transition both in the incidence and etiology of stroke. These changes, together with an array of social issues and institution of health care reform, are creating challenges for practicing neurologists throughout China. Notable problems include overcrowded, decrepit facilities, overloaded physician schedules, deteriorating physician-patient relationships, and an insufficient infrastructure to accommodate patients who need specialized neurologic care. Conversely, with the creation of large and sophisticated neurology centers in many cities across the country, tremendous opportunities exist. Developments in neurologic subspecialties enable delivery of high-quality care. Clinical and translational research based on large patient populations as well as highly sophisticated technologies are emerging in many neurologic centers and pharmaceutical companies. Child neurology and neurorehabilitation will be fast-developing subdisciplines. Given China's extensive population, the growth and progress of its neurology complex, and its ever-improving quality control, it is reasonable to anticipate that Chinese neurologists will contribute notably to unraveling the pathogenic factors causing neurologic diseases and to providing new therapeutic solutions. Copyright © 2011 by AAN Enterprises, Inc.


Niu W.,Shanghai JiaoTong University | Niu W.,CAS Shanghai Institutes for Biological Sciences | Niu W.,Shanghai Institute of Hypertension | Qi Y.,Capital Medical University
PLoS ONE | Year: 2011

Background: Mounting evidence has suggested that α-adducin and G-protein β3 (GNB3) genes are logical candidates for salt-sensitive hypertension. Some, but not all, studies have reported that α-adducin G460T and GNB3 C825T polymorphisms may influence the risk of the disease. To comprehensively address this issue, we performed a meta-analysis to evaluate the influence of these two polymorphisms on hypertension and potential biases in Chinese. Methods: Data were analyzed using Stata (v11.0) and random-effects model was applied irrespective of between-studies heterogeneity, which was evaluated via subgroup and meta-regression analyses. Study quality was assessed in duplicate. Publication bias was weighed using Egger's test and funnel plot. Results: 36 study populations totaling 9042 hypertensive patients and 8399 controls were finally identified. Overall, in allelic/genotypic/dominant/recessive models, no significant association was identified for both G460T and C825T polymorphisms (P>0.05) and there was possible heterogeneity (I2>25%). Subgroup analyses by study design indicated that the magnitude of association in population-based studies was marginally significantly strengthened for α-adducin G460T allelic model (OR = 1.12; 95% CI: 1:00-1.25; P = 0.043). Moreover, subgroup analyses by geographic distribution indicated comparison of 825T with 825C yielded a marginally significant increased risk in southern Chinese only (OR = 1.48; 95% CI: 1.01-2.16; P = 0.045). Further meta-regression analyses showed that geographic regions were a significant source of between-study heterogeneity for both polymorphisms. There was a possibility of publication bias for G460T, but not for C825T. Conclusions: Our overall results suggest null association of α-adducin G460T and GNB3 C825T polymorphisms with hypertension in Chinese but indicate local marginal significance of C825T, as a putative salt-sensitive switch, in southern Chinese. © 2011 Niu, Qi.


Ding L.,East Carolina University | Ding L.,Capital Medical University | Lu Z.,East Carolina University | Foreman O.,The Jackson Laboratory | And 5 more authors.
Gastroenterology | Year: 2012

Background & Aims: Integrity of the intestinal epithelium is required for nutrition absorption and defense against pathogens. Claudins are cell adhesion molecules that localize at tight junctions (TJs); many are expressed in the intestinal tract, but little is known about their functions. Claudin-7 is unique in that it has a stronger basolateral membrane distribution than other claudins, which localize primarily to apical TJs in the intestinal epithelium. We investigated the basolateral functions of claudin-7 and assessed the effects of disruption of Cldn7 in intestines of mice. Methods: We generated Cldn7 -/- mice and examined their intestines by histology, molecular and cellular biology, and biochemistry approaches. We performed gene silencing experiments in epithelial cell lines using small interfering RNAs (siRNAs). Results: The Cldn7 -/- mice had severe intestinal defects that included mucosal ulcerations, epithelial cell sloughing, and inflammation. Intestines of Cldn7 -/- mice produced significantly higher levels of cytokines, the nuclear factor κB p65 subunit, and cyclooxygenase 2; they also up-regulated expression of matrix metalloproteinases (MMPs)-3 and -7. siRNA in epithelial cell lines showed that the increased expression of MMP-3 resulted directly from claudin-7 depletion, whereas that of MMP-7 resulted from inflammation. Electron microscopy analysis showed that intestines of Cldn7 -/- mice had intercellular gaps below TJs and cell matrix loosening. Deletion of Cldn7 reduced expression and altered localization of the integrin α2 subunit in addition to disrupting formation of complexes of claudin-7, integrin α2, and claudin-1 that normally form in epithelial basolateral compartments of intestines. Conclusions: In mice, claudin-7 has non-TJ functions, including maintenance of epithelial cellmatrix interactions and intestinal homeostasis. © 2012 AGA Institute.


Kapila A.K.,University of Sfax | Watts H.R.,University of Sfax | Wang T.,Capital Medical University | Ma D.,University of Sfax
Journal of Alzheimer's Disease | Year: 2014

Alzheimer's disease (AD) is a major social and clinical burden in the elderly, affecting 5% of people aged over 65 and 20% aged over 80. Despite improved management, a cure has not been found and hence analysis of predisposing factors to identify preventive strategies has become increasingly important. Surgery and anesthesia have been proposed to increase the incidence of post-operative cognitive decline (POCD) and AD. This is hypothesized to be the result of a malignant neuroinflammatory response and subsequent synaptic impairment in the elderly and susceptible individuals. As a result, strategies are being explored to prevent surgery and anesthesia induced cognitive impairment. Whereas previously the diagnosis of AD was primarily dependent on clinical examination, biomarkers such as inflammatory cytokines, amyloid-β, and tau deposition in the cerebrospinal fluid have received increased attention. Nonetheless, AD is currently still treated symptomatically with acetylcholinesterase inhibitors and NMDA antagonists to improve cholinergic transmission and prevent glutamatergic excitotoxicity. Therapeutic success is, however, often not achieved, since these treatment methods do not address the ongoing neuroinflammatory processes and hence novel therapeutic and protective strategies are urgently needed. This review provides an insight into the current understanding of age-related cognitive impairment post-surgery and reflects on novel markers of AD pathogeneses exploring their use as targets for treatment. It gives a summary of recent efforts in preventing and treating POCD or AD with regards to the choice and depth of anesthesia, surgical strategy, and peri-operative medication, and discusses the mechanism of action and therapeutic prospects of novel agents. © 2014 - IOS Press and the authors. All rights reserved.


Yu J.,Capital Medical University
Investigative ophthalmology & visual science | Year: 2012

Macular pigment may protect against age-related macular degeneration (AMD) by its capacity to absorb blue light and scavenge free radicals. Current information on human macular pigment density has been largely from studies on Caucasian populations. The purpose of this study was to assess macular pigment density and its determinant factors in a Chinese population sample. Macular pigment optical density (MPOD) was measured in a healthy Chinese population using heterochromatic flicker photometry (HFP). Participants received a standard ophthalmic examination, and only subjects who were confirmed not to have any eye diseases except mild age-related cataract were included in the study. Demographic and lifestyle data and general health status were recorded by questionnaire. A total of 281 unrelated healthy Chinese individuals, including 96 males and 185 females, with ages ranging from 17 to 85 years, participated in the study. The mean and standard deviation of MPOD levels were 0.56 ± 0.19, 0.49 ± 0.18, 0.36 ± 0.15, and 0.19 ± 0.12, respectively, at 0.25°, 0.5°, 1.0°, and 1.75° eccentricity points. A significant age-related decline in MPOD was observed at 0.25° (P = 0.014). Females tended to have relatively lower levels of MPOD than males at 0.25° (P = 0.21), 0.5° (P = 0.025), and 1.0° (P = 0.16). No statistically significant association of MPOD was observed with body mass index or smoking status. Macular pigment density measured by HFP tended to decline with aging in this healthy Chinese population sample. Females may have lower levels of MPOD than males.


Chen J.,Peking University | Geng L.,Peking University | Song X.,Capital Medical University | Li H.,Beijing Shijintan Hospital | And 2 more authors.
Journal of Sexual Medicine | Year: 2013

Introduction: Atrophic vaginitis is a common occurrence, particularly among postmenopausal women; however, few seek or receive treatment. One therapeutic solution is topically applied products. Estrogen-based treatments have been shown to be effective; however, many patients are reluctant to use such formulations due to health concerns, hence the need to assess the efficacy of acceptable alternatives. Aim: This multicenter, randomized, controlled, open-label, parallel-group clinical trial set out to evaluate the efficacy and safety of hyaluronic acid vaginal gel to treat vaginal dryness compared with estriol cream in postmenopausal women. Methods: One hundred forty-four subjects were randomized, 72 to the test group treated with hyaluronic acid vaginal gel (Hyalofemme) and 72 to the control group treated with estriol cream (Ovestin). Treatment in both groups was applied by means of a device once every 3 days for a total of 10 applications over 30 days. Main Outcome Measures: Efficacy was measured by grading vaginal dryness and three other vaginal symptoms on a visual analog scale. Safety assessments included vital signs, laboratory examinations of the vaginal microecosystem, vaginal pH value, vaginal B ultrasound, and incidence of adverse events. Assessments were performed at baseline, by telephone after the third application, and at the final visit. Results: Both hyaluronic acid vaginal gel and estriol cream can significantly improve the clinical symptoms of vaginal dryness in postmenopausal women, with improvement rate of 84.44% and 89.42%, respectively, after 10 applications, without statistically significant difference between them. Conclusion: Both hyaluronic acid vaginal gel and estriol cream are effective in the treatment of vaginal dryness. Hyaluronic acid vaginal gel may be considered as a valid alternative to estrogen-based treatments in relieving the symptoms of vaginal dryness. © 2013 International Society for Sexual Medicine.


Wang X.H.,Capital Medical University
Zhonghua er ke za zhi. Chinese journal of pediatrics | Year: 2012

To study the clinical and laboratory features and diagnosis of the patient with anti-N-methyl-D-aspartate receptor(NMDAR)encephalitis in children. The data of clinical feature, laboratory findings, and radiological manifestation were reviewed and analyzed. Of the 7 patients, 4 were female and 3 were male. The age of onset was from 6.6 to 15.5 years (average 9.5 years). The onset of 4 cases started with convulsion. Six cases had seizures which was difficult to control by antiepileptic drugs. All patients had psychiatric symptoms and speech disorder. Six cases had different levels of decreased consciousness and dyskinesias. 6 cases had autonomic nerve instability, and 7 cases developed sleep disorders. The results of MRI examination were normal in all patients. The EEG of most patients showed focal or diffuse slow waves. Six cases had oligoclonal bands. All cases were confirmed to have the disease by detection of anti-NMDA receptor antibodies. No tumor was detected in any of the patients. All patients received immunotherapy. Anti-NMDAR encephalitis is a severe but treatable disorder that frequently affects children and adolescents. Pediatric patients had clinical manifestations similar to those of adult patients. But children have a lower incidence of tumors and hypoventilation also occurs less frequently in children. Most of children had a good prognosis.


Ghofrani H.-A.,Justus Liebig University | Ghofrani H.-A.,Imperial College London | D'Armini A.M.,University of Pavia | Grimminger F.,Justus Liebig University | And 9 more authors.
New England Journal of Medicine | Year: 2013

BACKGROUND: Riociguat, a member of a new class of compounds (soluble guanylate cyclase stimulators), has been shown in previous clinical studies to be beneficial in the treatment of chronic thromboembolic pulmonary hypertension. METHODS: In this phase 3, multicenter, randomized, double-blind, placebo-controlled study, we randomly assigned 261 patients with inoperable chronic thromboembolic pulmonary hypertension or persistent or recurrent pulmonary hypertension after pulmonary endarterectomy to receive placebo or riociguat. The primary end point was the change from baseline to the end of week 16 in the distance walked in 6 minutes. Secondary end points included changes from baseline in pulmonary vascular resistance, N-terminal pro-brain natriuretic peptide (NT-proBNP) level, World Health Organization (WHO) functional class, time to clinical worsening, Borg dyspnea score, quality-of-life variables, and safety. RESULTS: By week 16, the 6-minute walk distance had increased by a mean of 39 m in the riociguat group, as compared with a mean decrease of 6 m in the placebo group (least-squares mean difference, 46 m; 95% confidence interval [CI], 25 to 67; P<0.001). Pulmonary vascular resistance decreased by 226 dyn · sec · cm-5in the riociguat group and increased by 23 dyn · sec · cm-5in the placebo group (least-squares mean difference, -246 dyn · sec · cm-5; 95% CI, -303 to -190; P<0.001). Riociguat was also associated with significant improvements in the NT-proBNP level (P<0.001) and WHO functional class (P = 0.003). The most common serious adverse events were right ventricular failure (in 3% of patients in each group) and syncope (in 2% of the riociguat group and in 3% of the placebo group). CONCLUSIONS: Riociguat significantly improved exercise capacity and pulmonary vascular resistance in patients with chronic thromboembolic pulmonary hypertension. (Funded by Bayer HealthCare; CHEST-1 and CHEST-2 ClinicalTrials.gov numbers, NCT00855465 and NCT00910429, respectively.) Copyright © 2013 Massachusetts Medical Society.


Tapson V.F.,Duke University | Jing Z.-C.,Tongji University | Xu K.-F.,Peking Union Medical College | Pan L.,Capital Medical University | And 8 more authors.
Chest | Year: 2013

Background: Treprostinil is a stable prostacyclin analog approved for the treatment of pulmonary arterial hypertension (PAH) as parenteral or inhaled therapy. Treprostinil diolamine, a sustainedrelease oral formulation of treprostinil, was studied to determine whether it could provide a more convenient prostacyclin treatment option for patients with less severe PAH. The objective of this study was to evaluate the effi cacy and safety of oral treprostinil in patients with PAH receiving stable background endothelin receptor antagonist (ERA), phosphodiesterase type 5 inhibitor (PDE-5I) therapy, or both. Methods: A 16-week, multicenter, double-blind, placebo-controlled study in 310 patients with PAH compared bid administration of oral treprostinil (n = 157) with placebo (n = 153). The primary end point was change in 6-min walk distance at week 16. Secondary effi cacy end points were World Health Organization functional class, Borg dyspnea score, dyspnea-fatigue index, signs and symptoms of PAH, and clinical worsening. Results: One hundred thirty-two patients (84%) receiving oral treprostinil and 138 (90%) receiving placebo completed the study. The mean ± SD dose of oral treprostinil at week 16 was 3.1 ± 1.9 mg bid. The Hodges-Lehmann placebo-corrected median difference in 6MWD at week 16 was 10.0 m (95% CI, 2 2 to 22 m;P =.089). There were no signifi cant changes in secondary end points. The most common adverse events associated with oral treprostinil were headache (71%), diarrhea (55%), nausea (46%), fl ushing (35%), and jaw pain (25%). Conclusions: The addition of oral treprostinil to background ERA and PDE-5I therapy did not result in a statistically signifi cant improvement in exercise capacity. Side effects were common but tolerated by most subjects.


Yang M.,Capital Medical University | Zhao Y.,Peking Union Medical College
Journal of Craniofacial Surgery | Year: 2015

Full-thickness lower eyelid defect is one of common surgical diseases, which may lead to exposure keratopathy, corneal ulceration, and blindness. The aim of this study was to investigate the effect of superficial temporal artery island flap combined with auricular cartilage graft on the repair of full-thickness lower eyeliddefect. In this study, the reconstructions in 6 patients who had unilateral full-thickness lower eyelid defects due to ocular traumas or surgical resections of malignant tumors were carried out. The island flap of the frontal branch of superficial temporal artery reconstructed the outer layer and the total lower lid was supported with the plane of an auricular cartilage strip, offering a proper contour and physical strength to maintaina normal eyelid height. The follow-up time ranged from 6 to 24 months. No major complications including partial or total flap necrosis, signs of infection, venous congestion, and hematoma were seen in any of the patients, and all cases have been reconstructed well both aesthetically and functionally, showing esthetic eyelid contour, good color, and texture match. Superficial temporal artery island flap combined with auricular cartilage graft is a useful method functionally and cosmetically for the reconstructions of full-thickness lower eyelid defects because of its advantages including rich vascularity based on superficial temporal artery, wide pedicle rotational arc, which could be transferred throughout the face region, good eyelid contour with color and texture match, limited donor-site scar, and minimal postoperative morbidity. © 2015 Mutaz B. Habal, MD.


This prospective random control study was performed to compare the efficacy and safety of primary percutaneous coronary intervention (PCI) with biodegradable polymer (Excel) and with durable polymer (Cypher Select) sirolimus-eluting stents in patients with acute ST-elevation myocardial infarction (STEMI). Consecutive patients with STEMI underwent primary PCI were randomly divided into Cypher group (n = 113) and Excel group (n = 115). The primary endpoints were major adverse cardiac events (MACE, including death, reinfarction and target vessel revascularization) within 12 months. The second endpoints included late luminal loss and restenosis at 9 months. Angiographic follow-up data at 9 months were available in 43 (38%) patients in Cypher group and 48 (42%) in Excel group. The rates of in-stent restenosis and in-segment restenosis were 2.3% vs. 2.1% (P = 0.937) and 4.7% vs. 6.3% (P = 0.738), respectively. The late luminal loss of in-stent and in-segment were (0.17 ± 0.26) mm vs. (0.18 ± 0.33) mm (P = 0.483) and (0.19 ± 0.36) mm vs. (0.20 ± 0.42) mm (P = 0.419), respectively. There were no significant differences in death (3.5% vs. 2.6%, P = 0.692), reinfarction (1.8% vs. 2.6%, P = 0.658), target vessel revascularization (1.8% vs. 2.6%, P = 0.658), MACE (5.3% vs. 6.1%, P = 0.788) or stent thrombosis (4.4% vs. 3.5%, P = 0.692) at 12 months between Cyper group and Excel group. Excel and Cypher Select stents may have similar mid-term efficacy and safety in patients with STEMI treated with primary PCI.Further investigation is warranted to validate the long-term efficacy and safety.


There has not been sufficient evidence to support the Asians being less susceptible to pulmonary embolism (PE) than other ethnicities, because the prevalence of PE/deep venous thrombosis (DVT) in different racial and ethnic groups has not been carefully studied until recently except in Caucasians. To test the hypothesis that the Chinese population has a lower risk for PE, this study comprehensively assessed the hospital-based incidence and case fatality rates for PE during the 1997-2008 in China. A registration study of patients with suspected PE syndromes admitted to 60 level-3 hospitals involved in the National Cooperative Project for the Prevention and Treatment of Venous Thromboembolism (NCPPT) was conducted from January 1997 to December 2008. The only exclusion criterion was an age of less than 18 years. Helical computed tomography scan, ventilation-perfusion lung scintigraphy or pulmonary angiography was carried out before or after hospitalization. All images were reviewed and evaluated independently by two specialists. A total of 18,206 patients were confirmed with PE from 16,972,182 hospital admissions. The annual incidence was 0.1% (95% CI: 0.1% to 0.2%). The overall incidence of PE in male patients (0.2%, 95% CI: 0.1% to 0.3%) was higher than that in female patients (0.1% and 95% CI: 0.0% to 0.1%). An increasing incidence gradient for PE was noticed from Southern to Northern China. In addition, the case fatality rate was apparently decreasing: 25.1% (95% CI: 16.2% to 36.9%) in 1997 to 8.7% (95% CI: 3.5% to 15.8%) in 2008. Our findings suggest the relatively stable PE incidence and decreasing fatality trends in Chinese hospitals may be partially attributable to the implementation of the NCCPT and suggest the government should reevaluate the severity of PE so that health resources for the prevention, diagnosis and treatment of PE could be used to their fullest.


Huang R.H.,Peking University | Li X.P.,Capital Medical University | Rong Q.G.,Peking University
Science China Life Sciences | Year: 2013

Obstructive sleep apnea syndrome (OSAS) is characterized by recurrent collapses of the upper airway, which lead to repetitive transient hypoxia, arousals and finally sleep fragmentation. Both anatomical and neuromuscular factors may play key roles in the pathophysiology of OSAS. The purpose of this paper was to study the control mechanism of OSAS from the mechanical point of view. A three-dimensional finite element model was developed, which not only reconstructed the realistic anatomical structure of the human upper airway, but also included surrounding structures such as the skull, neck, hyoid, cartilage and soft tissues. The respiration process during the normal and apnea states was simulated with the fluid-structure interaction method (FSI) and the computational fluid dynamics method (CFD). The airflow and deformation of the upper airway obtained from the FSI and the CFD method were compared and the results obtained under large negative pressure during an apnea episode were analyzed. The simulation results show that the FSI method is more feasible and effective than the CFD method. The concave configuration of the upper airway may accelerate the collapse of the upper airway in a positive feedback mechanism, which supplies meaningful information for clinical treatment and further research of OSAS. © 2013 The Author(s).


Wu S.-X.,Beijing University of Chinese Medicine | Dong Z.-Y.,Capital Medical University
Current Vascular Pharmacology | Year: 2015

Objective: There are more than 300 million patients with hypertension in China, and at least 1 in 5 is using or has ever used Chinese Medicine (CM) treatment. Scope: This article reviews the efficacy and safety of CM as monotherapy and in combination with western medicine (WM) to explore its potential role in the clinical management of hypertension. Methods: Relevant articles were identified through PubMed, Chinese National Knowledge Infrastructure (CNKI) Database, VIP Chinese Journal Database, Wanfang Database, and China Biological Medicine Database (CBM-disc) search (up to 31 March, 2013). Findings: A total of 27 RCTs and 7 systematic reviews (including meta-analyses) were identified. These articles suggested that although as monotherapy, CM has limited effect for hypertension, while combined with WM, it does have a favorable effect of antihypertension. The combination therapy could not only improve the quality of life and the symptoms of hypertensive patients, such as dizziness and headache, but also stabilize blood pressure variability (BP). Moreover, the combined treatment of CM and WM may further reduce BP to the target levels for patients failed with hypertension control. Besides, the combination therapy also has more favorable effects than any WM monotherapy in protecting target organs as well as avoiding adverse reactions. Conclusion: When combined with WM, CM as a complementary treatment approach has certain effects for the control of hypertension and protection of target organs. However, more well-designed studies should be conducted to make a solid conclusion. © 2015 Bentham Science Publishers.


Du X.Y.,Capital Medical University
ILAR journal / National Research Council, Institute of Laboratory Animal Resources | Year: 2011

Mongolian gerbils (Meriones unguiculatus) have an incomplete circle of Willis (CoW), as a result of which approximately 30-40% of these animals develop focal cerebral ischemia after unilateral carotid occlusion (UCO). There are four types of patterns of the anterior and posterior communicating arteries (ACoAs and PCoAs, respectively) of the CoW and they determine the severity of the ischemic symptoms. We used 398 gerbils from five generations, including a selectively bred ischemia-prone group, to investigate post-UCO ischemic symptoms and possible correlations of ACoA and PCoA patterns between parents and their progeny. We observed that if the parents had complete ACoAs, their progeny also had complete ACoAs, and we found significant differences when the parents' ACoAs were incomplete: in 60.4% of offspring the type of ACoA was consistent with that of the mother and in 48.2% it was consistent with that of the father. The severity of the neurological symptoms after UCO was significantly related to the patterns of the ACoAs when PCoAs were absent. The proportion of UCO ischemia in gerbils with incomplete ACoAs was significantly higher than in those with complete ACoAs. After selectively breeding five generations, the proportion of UCO ischemia increased from 40% in the F1 animals to 75% in the F5 animals. Our results suggest that variations in the CoW are genetic and demonstrate that we successfully established an ischemia-prone group of gerbils.


Elliott M.,Australian Catholic University | Liu Y.,Capital Medical University
British Journal of Nursing | Year: 2010

Nurses are responsible for ensuring safety and quality of patient care at all times. Many nursing tasks involve a degree of risk, and medication administration arguably carries the greatest risk. Unfortunately, patients are frequently harmed or injured by medication errors. Some suffer permanent disability and for others the errors are fatal. Nurses have traditionally followed the five rights of medication administration (patient, drug, route, time, dose) to help prevent errors, and more recently, the seven rights (including documentation and reason). This article identifies nine rights of medication administration.


Qin Y.,Capital Medical University
Journal of the American Heart Association | Year: 2014

Coronary heart disease is the leading cause of death worldwide. Mitochondrial genetic determinants for the development of this disorder remain less explored. We performed a clinical and genetic evaluation and mutational screening of 22 mitochondrial tRNA genes in a cohort of 80 genetically unrelated Han Chinese subjects and 125 members of 4 families with coronary heart disease and 512 Chinese control subjects. This analysis identified 16 nucleotide changes among 9 tRNA genes. Of these, the T5592C mutation creates a highly conservative base pairing (5G-68C) on the acceptor stem of tRNA(Gln), whereas the G15927A mutation destabilizes a highly conserved base pairing (28C-42G) in the anticodon stem of tRNA(Thr). However, the other tRNA variants were polymorphisms. The pedigrees of BJH24 carrying the T5592C mutation, BJH15, and BJH45 harboring the G15927A mutation exhibited maternal transmission of coronary heart disease. Sequence analysis of their mitochondrial genomes revealed the presence of T5592C or G15927A mutation but the absence of other functionally significant mutations in all matrilineal relatives of these families. Our previous observations showed that altered structures of tRNAs by these mtDNA mutations caused mitochondrial dysfunction. These may be the first evidence that mtDNA mutations increase the risk of coronary heart disease. Our findings may provide new insights into the pathophysiology of this disorder.


Huang L.,Capital Medical University
Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons | Year: 2015

PURPOSE: Patients usually complain about numbness in the nasal tip after microfat injections. The present study evaluated the severity of the numbness in the nasal tip after the procedure.PATIENTS AND METHODS: To address the research question, a prospective study of young women was designed and performed at the Beijing Anzhen Hospital. Time was the primary predictor variable. The nasal tip sensation, which was evaluated using objective and subjective assessments, was used as the primary outcome variable. The McNemar-Bowker test (time vs nasal tip sensation) was used to detect statistical significance.RESULTS: A total of 30 young women (age 20.04 ± 3.63 years) were recruited for the present study. The preoperative mean touch threshold value was 3.60 units. One week after the injection, the women experienced a decrease in the touch threshold value by 2.50 units. However, the sensation recovered gradually during the follow-up period (1.51 units at week 2, 2.39 units at week 4, 3.01 units at week 8, and 3.35 units at week 12). Significant differences were detected between multiple different measurement points (P < .05). The percentage of those experiencing paresthesia after the microfat injections also gradually diminished to none.CONCLUSION: Postoperative numbness occurs in most patients receiving nasal microfat injections. Partial to complete recovery of nasal tip sensation can be expected to occur over a 3-month period. Copyright © 2015 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.


Yan L.Y.,Capital Medical University
Zhonghua fu chan ke za zhi | Year: 2011

To discuss protein marks expressed differentially in placenta of Down's syndrome by means of proteomics. We collected placenta of 18 patients (from March 2009 to December 2009 at Beijing Obstetrics and Gynecology Hospital), and divided them into two groups, one was 10 patients with fetal Down's syndrome, the other was normal pregnancies (normal chromosome) with other diseases. We separated proteins expressed in placentas of two groups by two-dimensional difference gel electrophoresis (2D-DIGE), and then analyzed the differential protein spots by software Decyder 6.5, then, spots differentially expressed by 1.5 fold or more were analyzed by matrix assisted laser desorption ionization-time of flight-mass spectrometry (MALDI-TOF-MS). In the end, the differential expressional levels of partially identified proteins were validated by western blot analysis. (1) Differential proteins of two groups protein spots of placentas separated by 2D-DIGE were analyzed by software Decyder 6.5 (these colored lights scattered in the image were protein spots), a total of 56 spots out of 352 were differentially expressed (P < 0.05) in two groups. We analyzed 17 protein spots (12 protein spots were over-expressed and 5 protein spots were down-expressed) differentially expressed by 1.5 fold or more by MALDI-TOF-MS. (2) Protein matching after searching protein database, 17 protein spots turn out to be 10 proteins. Four kinds [superoxide dismutase 1 (SOD1), peroxiredoxin 6 (PRDX6), heat shock protein 27 (HSP27), endoplasmic reticulum protein 29 (ERP29)] of them were validated by western blot analysis, the group of fetal Down's syndrome were 0.74 ± 0.12, 0.29 ± 0.10, 0.53 ± 0.16, 0.20 ± 0.09, the group of normal pregnancies were 0.51 ± 0.08, 0.34 ± 0.16, 0.18 ± 0.07, 0.35 ± 0.09, the results confirmed the observed changes in proteomics. Compared with normal pregnancies, there were differential proteins expressed in placenta of Down's syndrome. This approach might provide new screening markers in use for prediction of Down's syndrome, however, further study should be done to make these 4 proteins (SOD1, HSP27, ERP29, PRDX6) be new screening markers.


Li J.,Harbin Medical University | Ma W.,Capital Medical University | Wang S.,Harbin Medical University
Regulatory Peptides | Year: 2011

Gastrointestinal (GI) motility and gut hormones have been considered to be involved in the development and maintenance of obesity. Our aim was to assess the relationships between gastric emptying (GE), GI transit and gut hormones and leptin concentrations in diet-induced obese rat model. Male 6-week-old Sprague-Dawley rats were fed with a high-fat (HF) diet for 8. weeks to generate diet-induced obesity (DIO) and diet resistant (DR) rats. GE, GI transit and plasma ghrelin, cholecystokinin (CCK), PYY and leptin concentrations were determined in DIO, DR and control (CON) rats. The DIO rats had slower GE, higher plasma leptin and CCK concentrations, and lower plasma ghrelin concentration compared with CON and DR rats. GE was correlated with plasma ghrelin (r= 0.402, P= 0.028), CCK (r= - 0.518, P= 0.003) and leptin concentration (r= - 0.514, P= 0.004). The slower GE, which can be considered as an adaptive response aimed at HF diet induced obesity, may be mediated by changes of plasma ghrelin, CCK and leptin concentrations. © 2011 Elsevier B.V.


Sun Z.,Curtin University Australia | Xu L.,Capital Medical University
Computerized Medical Imaging and Graphics | Year: 2014

Computational fluid dynamics (CFD) is a widely used method in mechanical engineering to solve complex problems by analysing fluid flow, heat transfer, and associated phenomena by using computer simulations. In recent years, CFD has been increasingly used in biomedical research of coronary artery disease because of its high performance hardware and software. CFD techniques have been applied to study cardiovascular haemodynamics through simulation tools to predict the behaviour of circulatory blood flow in the human body. CFD simulation based on 3D luminal reconstructions can be used to analyse the local flow fields and flow profiling due to changes of coronary artery geometry, thus, identifying risk factors for development and progression of coronary artery disease. This review aims to provide an overview of the CFD applications in coronary artery disease, including biomechanics of atherosclerotic plaques, plaque progression and rupture; regional haemodynamics relative to plaque location and composition. A critical appraisal is given to a more recently developed application, fractional flow reserve based on CFD computation with regard to its diagnostic accuracy in the detection of haemodynamically significant coronary artery disease. © 2014 Elsevier Ltd.


Chen Z.-Y.,Peking University | Liang Q.-F.,Capital Medical University | Yu G.-Y.,Peking University
Cornea | Year: 2011

Purpose: To establish a rabbit model for keratoconjunctivitis sicca (KCS) to study autologous submandibular gland transfer for treating severe KCS. Methods: In 2 groups of 10 rabbits, left eyes were operated and right eyes were controls. In the trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were removed surgically; the palpebral and bulbar conjunctiva were swabbed with 50% trichloroacetic acid. In the non-trichloroacetic acid-treated group, the lacrimal and harderian glands and nictitating membrane were surgically removed. The Schirmer test was performed preoperatively and 1, 2, 3, and 4 months postoperatively. Corneal densities of rose bengal and fluorescein staining were scored every month postoperatively. At 4 months, the cornea and bulbar conjunctiva were removed from operated and control eyes for histopathology. The upper bulbar conjunctiva was used to determine goblet cell density. Results: Compared with preoperative conditions, tear secretion of operated eyes significantly decreased in both groups postoperatively, then gradually increased. Scores for corneal rose bengal and fluorescein staining were higher and conjunctival goblet cell density was lower in the operated eyes than in control right eyes in both groups, but no significant difference was found between the operated eyes of the two groups. Inflammatory histopathologic changes of the cornea and conjunctiva were not found in either of the eyes in the two groups. Conclusions: A new rabbit model for KCS could be created by either of these methods. Experimental KCS with reduction of tear production was possible with surgical ablation of the lacrimal and harderian glands and nictitating membrane. It is unnecessary to apply trichloroacetic acid to burn the conjunctiva. Our modified incision better exposed the surgical field. Copyright © 2011 by Lippincott Williams & Wilkins.


Huang L.,Capital Medical University
Transplantation Proceedings | Year: 2011

Purpose: Lidocaine is used to reduce the undesirable effects of ischemia because of its anti-inflammatory effects. Herein we investigated the effects of lidocaine on secondary ischemia in a skin flap model. Materials and Methods: In this epigastric skin flap protocol in animals, we followed 2 hours of primary global ischemia with a reperfusion period of 6 hours and then either secondary arterial or venous ischemia for another 6 hours during which we tested the usefulness of lidocaine. Lidocaine was injected via the intraperitoneal route 5 minutes before the second period of ischemia. The animals were allocated into secondary arterial ischemia or secondary venous ischemia groups which were subdivided according to the delivered agents. Neutrophil cell counts at the margins of the flaps were recorded 12 hours after the end of the second period of ischemia. Flap viability was assessed 1 week after the surgical procedure. Surviving flap area was recorded as the percentage of the whole area. The Least Significant Difference test was used to detect a significant difference among groups, and the Pearson test to evaluate the relationship between neutrophil counts and flap survival rate. Results: There were significant differences among groups both with respect to neutrophil count and flap survival. There was a relationship between the neutrophil counts and the flap survivals. Conclusion: Intraperitoneally injected lidocaine was an effective procedure to reduce flap necrosis as a cause of secondary ischemia in skin flaps, an effect of the ischemia-reperfusion injury. © 2011 Elsevier Inc.


Wang T.T.,Capital Medical University
Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi | Year: 2011

To investigate the role of Th17 cells in immune thrombocytopenia (ITP) mice model. ITP was induced by daily intraperitoneal injection of anti-platelet membrane CD41 antibody (MWReg30) into BALB/c mice, the mRNA expressions of Th17 cell associated transcription factors and cytokines in peripheral blood and spleen mononuclear cells were measured by real-time PCR, and the proportion of Th17 cells by FCM analysis. The percentage of Th17 cell was significantly elevated in ITP mice both in splenocyte and peripheral blood as compared with that in normal controls (P<0.01). ITP mice had elevated mRNA expressions of IL-17F, IL-17A and IL-6 in splenocyte (P<0.05), and of IL-21 in peripheral blood (P<0.05). There was a positive correlation between IL-17A and IL-17F (r = 0.934, P = 0.000), and between IL-17A/IL-17F and IL-6 (r = 0.598, P = 0.001; r = 0. 619, P = 0.000). Th17 cell might play an important role in the pathogenesis of ITP, at least involving in the clearance of platelets.


Zhao J.,Capital Medical University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2013

To study changes in quality of life in children after adenotonsillectomy for obstructive sleep apnea (OSA) documented by full night polysomnography. Children with OSA who fit the standard have been selected. They were asked to complete the OSA 18 quality of life survey before adenotonsillectomy and between 6 and 18 months after surgery. Scores from the preoperative and postoperative surveys were compared using the paired t test. The mean total OSA 18 score was 70.59 (SD=15.015) before surgery and 39.94 (SD=14.232) after surgery. The changes in total score, in the scores for sleep disturbance, physical symptoms, emotional symptoms, daytime functioning, and caregiver concerns, and for each item of the OSA 18 survey was highly significant (P<0.01). OSA has a relevant impact on quality of life and the children show a marked improvements after adenotonsillectomy.


Lester D.K.,Private Practice | Shantharam R.,University of Virginia | Zhang K.,Capital Medical University
Journal of Arthroplasty | Year: 2013

Quadriceps functional impairment accounts for a considerable amount of morbidity after knee replacement. Cruciate-retaining total knee arthroplasty (CR-TKA) may predispose to increased quadriceps demand. We compared bilateral relative quadriceps effort in seven patients with successful unilateral CR-TKA at least 2. years after surgery. Dynamic electromyography (EMG) recorded quadriceps activity during walking recorded simultaneously with gait analysis using IDEEA. There was greater EMG activity for the operated knees, both in amplitude (43.08±26.47 vs 16.02±5.38, P=.0355), and the area under the curve (7231.1±3869.8 vs. 2547.3±1007.9, P=.0267). The onset and duration of muscle activity were similar for both knees (P=.74). CR-TKA demonstrated threefold EMG activity and muscle effort during normal walking. The study suggested that quadriceps functional deficiency is associated with CR-TKA. © 2013 Elsevier Inc..


Wei W.-B.,Capital Medical University
Chinese Journal of Ophthalmology | Year: 2012

Optical coherence tomography (OCT) is a non-invasive, high-resolution imaging modality to acquire cross-sections and three-dimensional images of the microstructure in biological specimens. Because of rapid improvement of acquisition speed and axial resolution of OCT over recent years, OCT is becoming increasingly used in clinic for diagnosing or analyzing diseases of the eye, especially in the fundus. However, OCT has been used regardless of the location of lesions and the clarity of optical media. This misuse wastes resources and delays diagnosis and treatment of certain ocular diseases. Furthermore, the image of SD-OCT cannot be analyzed completely at present. Therefore, how to apply OCT in clinic appropriately and to analyze its image professionally is crucial, which should attract the attention of ophthalmologists. In addition, comprehensively analyzing ocular diseases combined with other examinations is extremely important to improve the level of diagnosis.


Zhao D.,Capital Medical University | Hu D.,Peking University
Nature Reviews Cardiology | Year: 2012

With the increasing globalization of clinical research and evidence, clinical-practice guidelines (CPGs) developed by the European Union (EU) and the USA are also becoming increasingly international. However, these CPGs can encounter barriers to their practical application. In this Perspectives article, we analyze the main obstacles to the application of EU and US CPGs for cardiovascular diseases from the unique perspective of China, and highlight some potential problems in the globalization of CPGs. Currently, China and other countries with limited independent evidence for CPG development must localize or adapt the CPGs developed by the EU, the USA, or international medical organizations, with systematic consideration of cost-effectiveness and alternative strategies on the basis of the available evidence from the native populations. At the same time, comprehensive capabilities to collect and review clinical evidence to produce population-specific CPGs should be developed. © 2012 Macmillan Publishers Limited. All rights reserved.


Weng C.-L.,Peking University | Zhao Y.-T.,Capital Medical University | Liu Q.-H.,Xiamen University | Fu C.-J.,Tsinghua University | And 4 more authors.
Annals of Internal Medicine | Year: 2010

Background: Noninvasive ventilation (NIV) is commonly used to treat patients with acute cardiogenic pulmonary edema (ACPE), but the findings of a recent large clinical trial suggest that NIV may be less effective for ACPE than previously thought. Purpose: To provide an estimate of the effect of NIV on clinical outcomes in patients with ACPE that incorporates recent trial evidence and explore ways to interpret that evidence in the context of preceding evidence that favors NIV. Data Sources: PubMed and EMBASE from 1966 to December 2009, Cochrane Central Register of Controlled Trials and conference proceedings through December 2009, and reference lists, without language restriction. Study Selection: Randomized trials that compared continuous positive airway pressure and bilevel ventilation with standard therapy or each other. Data Extraction: Two independent reviewers extracted data. Outcomes examined were mortality, intubation rate, and incidence of new myocardial infarction (MI). Data Synthesis: Compared with standard therapy, continuous positive airway pressure reduced mortality (relative risk [RR], 0.64 [95% CI, 0.44 to 0.92]) and need for intubation (RR, 0.44 [CI, 0.32 to 0.60]) but not incidence of new MI (RR, 1.07 [CI, 0.84 to 1.37]). The effect was more prominent in trials in which myocardial ischemia or infarction caused ACPE in higher proportions of patients (RR, 0.92 [CI, 0.76 to 1.10] when 10% of patients had ischemia or MI vs. 0.43 [CI, 0.17 to 1.07] when 50% had ischemia or MI). Bilevel ventilation reduced the need for intubation (RR, 0.54 [CI, 0.33 to 0.86]) but did not reduce mortality or new MI. No differences were detected between continuous positive airway pressure and bilevel ventilation on any clinical outcomes for which they were directly compared. Limitations: The quality of the evidence base was limited. Definitions, cause, and severity of ACPE differed among the trials, as did patient characteristics and clinical settings. Conclusion: Although a recent large trial contradicts results from previous studies, the evidence in aggregate still supports the use of NIV for patients with ACPE. Continuous positive airway pressure reduces mortality more in patients with ACPE secondary to acute myocardial ischemia or infarction. © 2010 American College of Physicians.


Qu H.,Capital Medical University
Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery | Year: 2012

To evaluate the protection of proximal colon segment by analyzing blood supply disorder of proximal colon segment during laparoscopic proctosigmoidectomy(11 cases) in the Chaoyang Hospital of Capital Medical University. It is concluded that the disorder of blood supply of proximal colon segment during laparoscopic proctosigmoid surgery has two reasons. One is the anatomic factor of mesenteric vessels; the other is the inappropriate operative procedure. It is recommended that left colonic artery should be retained, and inferior mesenteric artery should be handled at a low level, thus, the risk of proximal intestine blood supply disorder caused by vascular anatomy variation can be reduced.


Sun S.,Capital Medical University
Zhonghua wai ke za zhi [Chinese journal of surgery] | Year: 2013

To study the microsurgical decompression combined with cervical artificial disc replacement clinical efficacy for the treatment of cervical spondylosis. From January 2006 to November 2011, 21 cases of cervical spondylosis, totally 23 intervertebral spaces, were under the microscope disc decompression and cervical artificial disc replacement. There were 11 male and 10 female patients; aged from 28 to 60 years, with an average of 46.3 years. The diagnosis included 5 cases of nerve root type cervical spondylosis and 16 cases of cervical spondylotic myelopathy. Application of Bryan prosthesis treatment of 9 patients, a total of 10 intervertebral spaces; ProDisc-C prosthesis to treat 12 patients, a total of 13 intervertebral space. Following-up Japanese Orthopedic Association (JOA), neck disability index (NDI) and visual analogue scale (VAS) scores were recorded and compared with pre-operative scores by the paired t-test. The patients were followed up for 6 to 74 months, with an average of 27.7 months. Although a patient with spinal bony stenosis symptom improved, but not satisfied, and after the posterior decompression, who had a better prognosis. The remaining patients during follow-up symptoms were obvious improved, and the replacement segments were stable. There was no prosthesis subsidence and significantly offset. In 1 month post-operation and last follow-up compared with pre-operative scores, JOA (t = 9.195 and 17.070), NDI (t = 7.193 and 14.062) and VAS (t = 14.851 and 16.133) scores were significantly different (P < 0.05); and 1 month post-operation compared with last follow-up, JOA (t = 5.916), NDI (t = 7.722) and VAS (t = 4.564) scores were significantly different (P < 0.05). Cervical artificial disc replacement combined with microscopic decompression surgery can completely remove the oppression of nerve tissue caused by pressure, and the efficacy is more secure.


Wei A.-H.,Capital Medical University | Wei A.-H.,CAS Institute of Genetics and Developmental Biology | Li W.,CAS Institute of Genetics and Developmental Biology
Pigment Cell and Melanoma Research | Year: 2013

Hermansky-Pudlak syndrome (HPS) is an autosomal recessive and genetically heterogeneous disorder characterized by oculocutaneous albinism, bleeding tendency, and ceroid deposition, which likely leads to deleterious lesions in lungs, heart, and other organs. Currently, nine genes have been identified as causative for HPS in humans. Their pathological effects are attributable to the disrupted biogenesis of lysosome-related organelles (LROs) existing in multiple cell types or tissues, causing the pigmentory and non-pigmentory defects. This review focuses on the functional aspects of HPS genes in regulating LRO biogenesis and signal transduction. The understanding of these mechanisms expands our knowledge about the involvement of lysosomal trafficking in the targeting of cargoes for constitutive transport, degradation, and secretion. This opens an avenue to the pathogenesis of lysosomal trafficking disorders at the cellular and developmental levels. © 2012 John Wiley & Sons A/S.


Li Y.-W.,Tsinghua University | Chen Z.-G.,Beijing University of Chinese Medicine | Wang J.-C.,Nanjing Benq Hospital | Zhang Z.-M.,Capital Medical University
World Journal of Gastroenterology | Year: 2015

AIM: To evaluate the performance of superparamagnetic iron oxide (SPIO)-enhanced magnetic resonance imaging (MRI) in the detection and characterization of focal hepatic lesions (FHLs). METHODS: This meta-analysis compared relevant studies that were identified by searching PubMed, EMBASE, and the Cochrane Library databases for articles published between January 1988 and September 2014 and that met the following criteria: (1) SPIO-enhanced MRI was conducted to identify FHLs and data were sufficient for pooled analysis using Meta-DiSc 1.4; (2) hepatocellular carcinomas (HCCs) were differentiated from other FHLs; (3) well-differentiated HCCs (WDHCCs) were contradistinguished from dysplastic nodules; and (4) WD-HCCs were compared with moderately and poorly differentiated HCCs (MD-and PDHCCs, respectively). RESULTS: The data obtained from 15 eligible studies yielded a sensitivity of 85% and a specificity of 78% for differentiating between HCCs and other FHLs. The sensitivity was unchanged and the specificity was increased to 87% when non-HCC malignancies were excluded. Comparative analyses between WD-HCCs and MD-and PD-HCCs from seven studies showed a sensitivity of 98% and a specificity of 50% for the diagnosis of MD-and PD-HCCs, and the area under the summary receiver operating characteristics (sROC) curve was 0.97. A comparison between WD-HCCs and dysplastic nodules revealed a sensitivity of 50% and a specificity of 92% for the diagnosis of WD-HCCs and the area under the sROC curve was 0.80. CONCLUSION: SPIO-enhanced MRI is useful in differentiating between HCCs and other FHLs. © The Author(s) 2015. Published by Baishideng Publishing Group Inc. All rights reserved.


Wei C.,Capital Medical University
European Archives of Oto-Rhino-Laryngology | Year: 2016

We aimed to systematically review the randomized controlled trials (RCTs) regarding the therapeutic efficiency of proton pump inhibitor (PPI) therapy for laryngopharyngeal reflux (LPR). Randomized and placebo-controlled trials regarding the therapeutic efficacy of PPIs on LPR patients were systematically searched from MEDLINE, Cochrane Library, and EMBASE. Data were extracted from eligible studies meeting the inclusion criteria. Heterogeneity among these eligible studies was evaluated by the Q-statistic and I2 test, based on which a fixed- or random-effects model was performed to pooled relative risks (RRs) for the response rate and standardized mean differences (SMDs) for reflux symptom index (RSI) and the reflux finding score (RFS). Potential publication bias was evaluated by trim and fill method. Totally, 13 RCTs including 831 LPR patients were eligible for this meta-analysis. Pooled results demonstrated that the total RSI significantly improved for patients who received PPI therapy by comparing with those receiving placebo (SMD = 3.65; 95 % CI 1.56–5.75), though no significant difference was found in response rate (RR = 0.04, 95 % CI −0.06 to 0.14) and RFS (SMD = 0.91; 95 % CI −0.53 to 2.35) between these two groups of patients. No publication bias was found among eligible studies. PPI treatment could significantly improve reflux symptoms in LPR patients and, therefore, should be taken into consideration for LPR management with other strategies, such as lifestyle modification. © 2016 Springer-Verlag Berlin Heidelberg


Huang X.F.,Capital Medical University
The Chinese journal of dental research : the official journal of the Scientific Section of the Chinese Stomatological Association (CSA) | Year: 2010

Members of the transforming growth factor-β (TGF-β) superfamily are critical regulators that control cell proliferation, differentiation and apoptosis. TGF-β signalling also regulates the morphogenesis of many developing organs. The development of mouse tooth germ, which is a good model for organogenesis, provides a powerful tool for elucidating the molecular mechanisms that control organogenesis. As ectodermal appendages, the tooth organ arises from complex and progressive interactions between an ectoderm, the oral epithelium and an underlying mesenchyme. Their morphogenesis is regulated by conserved signalling pathways, including TGF-β. In this review, the essential function of the TGF-β superfamily will be discussed in detail, including TGF-β, bone morphogenetic proteins (BMP), activin, etc, during tooth crown patterning and following tooth root development. The review also highlights recent advances in the understanding of Smad-dependent and Smad-independent pathways in regulating tissue-tissue interactions during patterning of tooth crown and root.


Li Y.,Peking Union Medical College | Wang Q.,Capital Medical University | Wang Q.,Institute of Infectious Diseases | Yao X.,Peking Union Medical College
European Journal of Pharmacology | Year: 2010

The herbal products baicalin, baicalein, chlorogenic acid, and ginsenoside Rf have multiple pharmacological effects and are extensively used in alternative and/or complementary therapies. The present study investigated whether baicalin, baicalein, chlorogenic acid, and ginsenoside Rf induced the expression of the cytochrome P450 3A4 (CYP3A4) and multi-drug resistance 1 (MDR1) genes through the pregnane X receptor and constitutive androstane receptor pathways. Real time PCR, western blotting, and a luminescent assay were used to assess the induction of gene expression and activity of CYP3A4 and MDR1 by the test compounds. The interactions of baicalein/chlorogenic acid/ginsenoside Rf with constitutive androstane receptor and pregnane X receptor were evaluated using luciferase reporter and gel shift assays. Baicalein induced the expression of CYP3A4 and MDR1 mRNA by activating pregnane X receptor and constitutive androstane receptor. Chlorogenic acid and ginsenoside Rf showed a relatively weak effect on CYP3A4 promoter activation only in HepG2 cells cotransfected with constitutive androstane receptor and demonstrated no effects on MDR1 via either the constitutive androstane receptor or pregnane X receptor pathway. Baicalin had no effect on either CYP3A4 or MDR1 gene expression. In conclusion, baicalein has the potential to up-regulate CYP3A4 and MDR1 through the direct activation of the constitutive androstane receptor and pregnane X receptor pathways. Chlorogenic acid and ginsenoside Rf only induced constitutive androstane receptor-mediated CYP3A4 expression. © 2010 Elsevier B.V.


Wang S.-J.,Chinese Academy of Sciences | Yang H.-Y.,Capital Medical University | Xu G.-S.,PLA Fourth Military Medical University
Evidence-based Complementary and Alternative Medicine | Year: 2012

Here we used a mouse model of zymosan-induced colorectal hypersensitivity, a similar model of IBS in our previous work, to evaluate the effectiveness of the different number of times of acupuncture and elucidate its potential mechanism of EA treatment. Colorectal distension (CRD) tests show that intracolonic zymosan injection does, while saline injection does not, induce a typical colorectal hypersensitivity. EA treatment at classical acupoints Zusanli (ST36) and Shangjuxu (ST37) in both hind limbs for 15 min slightly attenuated and significantly blunted the hypersensitive responses after first and fifth acupunctures, respectively, to colorectal distention in zymosan treatment mice, but not in saline treatment mice. Western blot results indicated that ion channel and TrpV1 expression in colorectum as well as ERK1/2 MAPK pathway activation in peripheral and central nerve system might be involved in this process. Hence, we conclude that EA is a potential therapeutic tool in the treatment and alleviation of chronic abdominal pain, and the effectiveness of acupuncture analgesia is accumulative with increased number of times of acupuncture when compared to that of a single time of acupuncture. © 2012 Shao-Jun Wang et al.


Zhang Q.,University of Sichuan | Zhou Y.,Capital Medical University | Yu C.-M.,Chinese University of Hong Kong
Current Opinion in Cardiology | Year: 2015

Purpose of review Cardiac resynchronization therapy (CRT) reduces morbidity and mortality in patients with mild-to-severe heart failure. However, up to 40% of CRT recipients are nonresponders. This review addresses important aspects with regard to the identification and management of CRT nonresponders.Recent findings Mid-term clinical or echocardiographic nonresponse is associated with worse clinical outcomes during the extended follow-up. A number of predictors are indicative of CRT response, which include patient characteristics, electrical determinants, and imaging techniques from preimplant to postimplant period, and can be grouped as modifiable and nonmodifiable contributors to treatment response. Advanced age, male sex, ischemic cause, end-stage heart failure, inadequate electrical delay, and absence of mechanical dyssynchrony are regarded as unfavorable but nonmodifiable factors, for which considering underutilization of CRT by refining patient selection is reasonable. On the contrary, more efforts should be made to optimize patient management by correcting those modifiable factors, such as suboptimal medical therapy, uncontrolled atrial fibrillation, left ventricular lead dislodgement or inappropriate location, loss of biventricular capture, and lack of device optimization.Summary Proper management and careful selection of CRT recipients will transform a proportion of treatment nonresponders into responders, which is vital to improve patients' outcome. © 2014 Wolters Kluwer Health | Lippincott Williams & Wilkins.


Tian N.,Capital Medical University | Cao Z.,Renmin University of China | Zhang Y.,Peking University
Neuroscience Bulletin | Year: 2014

MicroRNA alterations have been reported in patients with Alzheimer's disease (AD) and AD mouse models. We now report that miR-206 is upregulated in the hippocampal tissue, cerebrospinal fluid, and plasma of embryonic APP/PS1 transgenic mice. The increased miR-206 downregulates the expression of brain-derived neurotrophic factor (BDNF). BDNF is neuroprotective against cell death after various insults, but in embryonic and newborn APP/PS1 mice it is decreased. Thus, a specific microRNA alteration may contribute to AD pathology by downregulating BDNF. © 2014 Shanghai Institutes for Biological Sciences, CAS and Springer-Verlag.


Wang G.,Capital Medical University | Gislum M.,Lundbeck | Filippov G.,Lundbeck | Montgomery S.,Imperial College London
Current Medical Research and Opinion | Year: 2015

Objective: This randomized, double-blind 8 week study compared the efficacy and tolerability of fixed-dose treatment with vortioxetine (10mg/day) and venlafaxine extended release (XR) (150mg/day) in major depressive disorder (MDD) patients. Research design and methods: Patients aged 18-65 years with a primary diagnosis of recurrent MDD, a Montgomery-Åsberg Depression Rating Scale (MADRS) total score ≥26 and a Clinical Global Impression-Severity (CGI-S) score ≥4 were randomized (1:1) to treatment with either vortioxetine or venlafaxine XR. The primary endpoint was change from baseline to Week 8 in MADRS total score (analysis of covariance [ANCOVA], full-analysis set [FAS], last observation carried forward [LOCF]), using a non-inferiority margin of +2.5 points. Pre-specified secondary endpoints included MADRS response and remission rates, anxiety symptoms (HAM-A), CGI, overall functioning (SDS), and health-related quality of life (Q-LES-Q). Clinical trial registration: This study (SOLUTION) has the www.ClinicalTrials.gov identifier: NCT01571453. Results: On the primary efficacy endpoint at Week 8, non-inferiority was established with a difference of -1.2 MADRS points in favor of vortioxetine (95% CI: -3.0 to 0.6). The MADRS total score decreased (improved) from 32.3±4.6 at baseline to 13.6±9.6 (vortioxetine: n=209) and from 32.3±4.5 to 14.8±10.4 (venlafaxine XR: n=215) (FAS, LOCF). At Week 8, the HAM-A and SDS total scores, CGI and Q-LES-Q scores, and response and remission rates demonstrated similar improvement for vortioxetine and venlafaxine XR, with remission rates (MADRS ≤10) of 43.1% (vortioxetine) versus 41.4% (venlafaxine XR) (LOCF). Fewer vortioxetine than venlafaxine XR patients withdrew for any reason (18.0% versus 27.4%) or for adverse events (6.6% versus 13.7%). The most frequent adverse events (≥5%) for both treatments were nausea, dizziness, headache, and dry mouth. In addition, accidental overdose, decreased appetite, constipation and insomnia were reported by (≥5%) of patients treated with venlafaxine XR. Limitations: The inclusion and exclusion criteria may limit the generalizability of the study. Since patients with a history of lack of response to venlafaxine XR were excluded from this study, there is a selection bias in favor of venlafaxine XR. Conclusion: Vortioxetine was at least as efficacious as venlafaxine XR and was safe and better tolerated than venlafaxine XR. © 2015 All rights reserved: reproduction in whole or part not permitted.


To observe the short-term and long-term impacts of in-hospital pneumonia on outcomes of patients hospitalized with acute ischemic stroke. All consecutive patients older than 18 years with acute ischemic stroke were prospectively recruited to this study, including 132 clinical centers in 32 provinces and 4 municipalities (including Hong Kong region) in China from September 2007 to August 2008. Case report form was designed. Data of pneumonia and survival outcomes at baseline; discharge; 3, 6 and 12 months after admission were recorded. Multivariable logistic regression was used for statistical correlation analysis. A total of 1373 (11.88%) patients from 11 560 acute ischemic stroke patients were notified with in-hospital pneumonia. The case fatality rate was 14.4% (1664 patients) within 12 months after stroke onset. The fatality rate in patients with pneumonia was higher than that of patients without pneumonia.In-hospital pneumonia was an independent risk factor for death at discharge (adjusted OR = 5.916; 95%CI 4.470-7.831), at 3 months (adjusted OR = 3.641; 95%CI 3.035-4.367), 6 months (adjusted OR = 3.445; 95%CI 2.905-4.086), and 12 months (adjusted OR = 3.543; 95%CI 3.016-4.161) after onset. In-hospital pneumonia is an adverse factor for the short-term and long-term survival of acute ischemic patients in China.


Wang W.,Capital Medical University
Zhonghua xin xue guan bing za zhi | Year: 2010

To describe the distribution characteristics of carotid atherosclerosis and to analyze its association with cardiovascular risk factors in middle-aged and elderly Chinese. In 2007, the second cross-sectional examination for cardiovascular risk factors and B-mode ultrasound imaging on carotid arteries were performed in a Chinese population consisting of 2681 subjects aged 43-81 years old from two cohorts participants of the People's Republic of China/United States of America collaborative study (USA-PRC study) and the Chinese multi-provincial cohort study (CMCS). The association of cardiovascular risk factors with intima-media thickening (ITM) and plaque of carotid arteries was analyzed. (1) The mean intima-media thickening (Mean-IMT) of carotid artery was 0.68 mm, the maximal intima-media thickening (Max-IMT) was 1.07 mm. (2) The prevalence rate of carotid plaque was 60.3% in all subjects. As for the different sites of carotid arteries, the plaque prevalence rate at carotid bulb was 61.2% for male and 51.6% for female (chi2 = 23.44, P < 0.01), and at the internal carotid artery was 24.7% for male and 12.2% for female (chi2 = 69.57, P < 0.01), at the common carotid artery was 20.9% for male and 13.8% for female (chi2 = 23.18, P < 0.01). (3) After adjusting for age and other cardiovascular risk factors, the Mean-IMT and Max-IMT increased with elevated levels of systolic blood pressure, plasma glucose and low density lipoproteins cholesterol (LDL-C) (all P < 0.05). Hypertension, diabetes, smoking and high LDL-C (P < 0.05) were independent predict factors for the risk of carotid plaque. The carotid atherosclerosis was common in middle and older age Chinese and was positively associated with classical cardiovascular disease risk factors.


Ye Y.P.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2012

To assess the diagnostic predictive value of Wells score and modified Geneva score for acute pulmonary embolism by prospective case series and to explore a more suitable scoring system for Chinese population. All the patients suspected of pulmonary embolism (PE) and received CT pulmonary angiography (CTPA) were enrolled consecutively in Fuxing Hospital, Capital Medical University, China, from June 2009 to August 2011. Before CTPA test or on condition that test results were unknown, clinical scoring was assessed prospectively by the Wells score and the modified Geneva score. The probability of PE in each patient was assessed and the patients were divided into low, moderate and high probability groups according to the clinical scores. The result of CTPA was used as the diagnostic gold standard for PE. Diagnostic accuracy in each group was analyzed. The predictive accuracy of both scores was compared by AUC(ROC) curve. A total of 139 patients met our enrollment criteria and 117 eligible patients entered our study at last. PE was diagnosed in 47 patients by CTPA with an overall prevalence of 40.2%.Prevalence of PE in the low, moderate and high pretest probability groups assessed by the Wells score and by the simplified modified Geneva score were 7.1% (3/42), 42.9% (21/49), 88.5% (23/26) and 10.0% (3/30), 48.1% (37/77), 7/10, respectively. AUC(ROC) curves for the Wells score and the simplified modified Geneva score were 0.872 (95%CI 0.810 - 0.933) and 0.734 (95%CI 0.643 - 0.825) respectively, with a significant difference (P = 0.005). The Wells score is more accurate for clinical predicting acute PE than the modified Geneva score.


Yang J.,Capital Medical University
Clinical nuclear medicine | Year: 2012

An 8-year-old boy with history of embryonal rhabdomyosarcoma resected 2 years ago presented with abdominal pain and elevated pancreatic enzymes. Acute pancreatitis was diagnosed, and appropriate therapy was started. An FDG PET/CT scan revealed a hypermetabolic region in the pancreas suspicious for malignancy; however, the patient's condition improved after therapy. A repeat PET/CT scan performed 3 weeks later for recurrent symptoms showed interval disease progression, consistent with metastatic disease. Our case indicates that pancreatitis can accompany malignancy or be related to the obstruction caused by tumor, and patient's response to therapy for acute pancreatitis does not rule out malignancy.


Wu D.,China Japan Friendship Hospital | Wang J.,China Japan Friendship Hospital | Yuan Y.,Capital Medical University
Neuroscience Letters | Year: 2015

This study aimed to investigate the effects of anodal transcranial direct current stimulation (A-tDCS) over the left posterior perisylvian region (PPR) on picture naming and cortical excitability measured with electroencephalography (EEG) nonlinear dynamics analysis (NDA) in aphasic patients. Twelve aphasic patients received 20 sessions of speech-language therapy during each of three phases: sham tDCS (Phase A1); A-tDCS to the left PPR (Phase B); and sham tDCS (Phase A2). Picture naming and auditory word-picture identification were measured before and after each phase. The EEG nonlinear index of approximate entropy (ApEn) was calculated for all subjects and 12 normal controls. Picture naming and auditory word-picture identification was significantly improved after phase B. The EEG ApEn analysis indicated that improved picture naming correlated with a higher activation level in wide areas of the left hemisphere and in isolated areas of the right hemisphere after phase B. These results revealed that A-tDCS over the left PPR coupled with speech-language therapy can improve picture naming and auditory comprehension in aphasic patients. tDCS not only modulates activity in the brain region directly underlying the stimulating electrode but also in a network of brain regions that are function-related. © 2015 .


Dong N.,Tianjin Medical University | Tang X.,Tianjin Medical University | Xu B.,Capital Medical University
Investigative Ophthalmology and Visual Science | Year: 2015

PURPOSE. MicroRNA-181a (miR-181a) is thought to be involved in posterior capsule opacification (PCO). This study investigated the role of miR-181a in the proliferation, migration, and epithelial–mesenchymal transition (EMT) of lens epithelial cells (LECs). METHODS. The expression of miR-181a was detected in human PCO-attached LECs and LECs obtained from patients with anterior polar cataracts by quantitative RT-PCR (qRT-PCR). The proliferation of SRA01/04 cells transfected with miR-181a mimics was analyzed by MTT assays and bromodeoxyuridine (BrdU)-incorporation assays. The migration of SRA01/04 cells was evaluated by wound-healing assays and Transwell migration. Luciferase reporter assays were used to validate the regulation of a putative target of miR-181a. RESULTS. The expression of miR-181a is decreased in human PCO-attached LECs and LECs obtained from patients with anterior polar cataracts. A significant decrease in proliferation was observed in SRA01/04 cells transfected with miR-181a mimics. The overexpression of miR-181a inhibited the migration ability of LECs. Downregulation of fibronectin, Slug, and cyclooxygenase-2 (COX-2) expression and upregulation of E-cadherin expression were induced in human PCO-attached LECs transfected with miR-181a mimics and miR-181a– overexpressing LECs obtained from patients with anterior polar cataracts. Furthermore, luciferase assays using a reporter carrying a putative miR-181a target site in the 3′ untranslated region of c-Met, Slug, and COX-2 revealed that miR-181a directly targets c-Met, Slug, and COX- 2. CONCLUSIONS. These data reveal that miR-181a can inhibit the proliferation, migration, and EMT of LECs and suggest that the restoration of miRNA-181a expression may be a potential novel therapeutic target for the prevention and treatment of posterior capsule opacification. © 2015 The Association for Research in Vision and Ophthalmology, Inc.


Wang F.,Capital Medical University
Neurobiology of aging | Year: 2012

Alzheimer's disease (AD) has a complex pattern of inheritance and many genes have recently been reported to contribute to the disease susceptibility. We selected 106 SNPs within 16 candidate genes and performed a multistage association study using 4 sample sets consisting of 731 AD patients and 738 control subjects to identify genetic factors for AD in Han Chinese. A single nucleotide polymorphism (SNP) in the insulin degrading enzyme gene (IDE), rs3781239, showed a significant association with AD. The C allele increased the risk of AD 1.72-fold than the G allele (odds ratio [OR] = 1.72, 95% confidence interval [CI] = 1.17-2.53, p = 0.006) and CC carriers had a 4.89-fold higher risk for AD than that of the carriers with CG and GG genotypes (odds ratio = 4.89, 95% CI = 1.85-12.91, p = 0.001). Moreover, the CC genotype was significantly associated with earlier age at onset (p = 0.001, hazard ratio [HR] = 2.09, 95% CI = 1.38-3.18). Our data suggest that the polymorphism of IDE is associated with susceptibility to Alzheimer's disease in Han Chinese. Copyright © 2012 Elsevier Inc. All rights reserved.


Zhu J.,Capital Medical University
Chinese medical journal | Year: 2012

Warfarin is the most commonly prescribed anticoagulant worldwide. Factors which influence warfarin's inter-individual requirements including age, weight, and genetic factors explained about 50% of dose variance, and unidentified factors still remain. The aim of this study was to explore whether white blood cell count affects warfarin dose requirements. Three hundred and twenty-two patients suffering from venous thromboembolism (VTE) and taking warfarin were recruited in this study. Genotyping of selected genes was conducted and other information was collected using the Epidata software. Dosing algorithms were constructed by multivariate linear regression analyses. In addition to well-known factors such as age, body weight, CYP2C9*3, and VKORC1 c.1173C > T, white blood cell counts negatively related to warfarin dose requirements and contributed to warfarin variability in Han Chinese by about 0.6%. White blood cell count has a small but significant contribution to warfarin dose requirements in Han Chinese.


Yang X.,University of Cardiff | Yang X.,Capital Medical University | Martin T.A.,University of Cardiff | Jiang W.G.,University of Cardiff
International Journal of Oncology | Year: 2012

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin superfamily which has been indicated in the pathophysiology of the nervous system and is important in a number of neurological and psychological conditions. Recently, BDNF was also shown to play a role in the development and progression of solid tumour myeloma. It has been reported that BDNF is aberrantly expressed in human breast cancer and that a raised level of BDNF is associated with poor clinical outcome and reduced survival. The present study investigated the role of BDNF in human breast cancer. A panel of human breast cancer cells was used and the expression profile of BDNF was evaluated using RT-PCR. We constructed a set of anti-BDNF transgenes which were used to transfect breast cancer cells in order to generate BDNF knocked down cells. The impact of BDNF knockdown on growth and apoptosis was evaluated. Statistical analysis was performed using SPSS. P<0.05 was considered statistically significant. BDNF gene transcripts were successfully detected in the breast cancer cell lines MCF-7, MDA-MB-231 and ZR75-1 MDA-MB-231 and MCF-7 wild-type cells were subject to transfection of anti-BDNF transgenes, followed by the establishment of BDNF knocked down sublines. Knockdown of BDNF in MDA-MB-231 and MCF-7 cell lines resulted in decreased rates of growth and proliferation. Analysis of apoptosis showed that cell apoptosis was increased in cells stably transfected with ribozymes for BDNF compared with the vector control. It is concluded that BDNF, a neurotrophic growth factor aberrantly expressed in cancers such as breast cancer, has a profound impact on the cellular behaviour of breast cancer cells and that BDNF is associated with a reduction of the apoptosis of breast cancer. BDNF is, therefore, a potential therapeutic target in breast cancer and its effect in human breast cancer requires further investigation.


Feng K.,CAS Technical Institute of Physics and Chemistry | Xie N.,Capital Medical University | Chen B.,CAS Technical Institute of Physics and Chemistry | Zhang L.-P.,CAS Technical Institute of Physics and Chemistry | And 2 more authors.
Macromolecules | Year: 2012

A new set of amphiphilic random copolymers has been constructed directly by hydrophilic, hydrophobic, and functional spiropyran monomers in one-pot ROMP. The resulting copolymers are able to self-assemble in water to polymeric micelles, which exhibit reversible disruption and regeneration characteristics upon ultraviolet (UV) and visible-light irradiation. When hydrophobic dyes Nile red, which served as a model for drug delivery, are encapsulated within the core of polymeric micelle, their releasing and reloading have been realized by exposure to UV and visible light, respectively, in the aqueous solution. © 2012 American Chemical Society.


Dunning J.,Imperial College London | Baillie J.K.,Roslin Institute | Cao B.,Capital Medical University | Hayden F.G.,University of Virginia
The Lancet Infectious Diseases | Year: 2014

Observational data suggest that the treatment of influenza infection with neuraminidase inhibitors decreases progression to more severe illness, especially when treatment is started soon after symptom onset. However, even early treatment might fail to prevent complications in some patients, particularly those infected with novel viruses such as the 2009 pandemic influenza A H1N1, avian influenza A H5N1 virus subtype, or the avian influenza A H7N9 virus subtype. Furthermore, treatment with one antiviral drug might promote the development of antiviral resistance, especially in immunocompromised hosts and critically ill patients. An obvious strategy to optimise antiviral therapy is to combine drugs with different modes of action. Because host immune responses to infection might also contribute to illness pathogenesis, improved outcomes might be gained from the combination of antiviral therapy with drugs that modulate the immune response in an infected individual. We review available data from preclinical and clinical studies of combination antiviral therapy and of combined antiviral-immunomodulator therapy for influenza. Early-stage data draw attention to several promising antiviral combinations with therapeutic potential in severe infections, but there remains a need to substantiate clinical benefit. Combination therapies with favourable experimental data need to be tested in carefully designed aclinical trials to assess their efficacy. © 2014 Elsevier Ltd.


Bai H.Y.,Capital Medical University
Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi | Year: 2011

This study was to examine the relation of peripheral arterial disease (PAD) and renal insufficiency in cardiovascular patients with high risk. PAD, defined as an ankle brachial index (ABI) ≤ 0.9 in either leg and renal insufficiency, defined as an estimated creatinine clearance (CRCL) < 60 ml×min(-1)×1.73 m(-1) were evaluated in 5270 Chinese patients at high risk of CV. 2648 (50.2%) patients had an estimated CRCL < 60 ml×min(-1)×1.73 m(-1), while 746 (14.2%) patients having PAD defined ABI ≤ 0.9. ABI and CRCL were positively correlated (r = 0.217, P < 0.001). The prevalence of PAD in patients with renal dysfunction was significantly higher than those with normal renal function (19.9% vs. 8.3%, P < 0.001). The association of ABI ≤ 0.9 with CRCL was independent from potential confounders such as age, diabetes, hypertension, hypercholesterolemia, smoking, coronary artery disease and stroke history (OR = 0.98, 95%CI: 0.98 - 0.99, P < 0.001). Results from the present study demonstrated a remarkably high prevalence of PAD (defined as an ABI ≤ 0.9) among patients with renal dysfunction and at high risk. Even after adjustment for important confounders such as age, diabetes, and coronary artery and cerebro-vascular diseases etc., persons with lower CRCL were still more likely to have an ABI ≤ 0.9.


Jia L.,Capital Medical University
Advances in Experimental Medicine and Biology | Year: 2015

Kidney function is rarely studied in the context of blood kidney and urine as a system. Kidney can be considered as a black box, while plasma and urine proteomes closely represent the protein compositions of the input and output of the kidney. This idea provides a new approach for studying organ functions with a proteomic methodology. Because of its distinctive input (plasma) and output (urine), it is reasonable to predict that the kidney will be the first organ whose functions are further elucidated by proteomic methods in the near future. © Springer Science+Business Media Dordrecht 2015.


Cui J.,Capital Medical University
Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases | Year: 2012

To assess the efficacy and safety of topical application of mitomycin-C in the management of benign cicatricial airway stenosis (BCAS), and to discuss the feasibility of catheter drip to achieve the topical application of mitomycin-C. Twenty patients with BCAS were divided into control group and experimental group (topical application of mitomycin-C). Patients in control group were treated only with interventional bronchoscopy which including balloon dilation, argon plasma coagulation/electrotomy, cryotherapy, stent placement and so on, while patients in experimental group were treated with interventional bronchoscopy adjuncted with topical application of mitomycin-C (0.4 mg/ml). According to the length of airway lesion, the dosage was 1 ml/cm. Topical application of mitomycin-C was achieved by catheter drip. Photographs were taken at the place above the proximal end of airway stenosis and a software called Digimizer was used to measure the cross sectional area of stenosed airway. A 3-month follow-up was done to observe the safety and curative effect of topical application mitomycin-C in airway. Ten patients (3 male and 7 female) ranging in age from 20 to 71 years [mean (36 ± 17) years] enrolled in control group and 10 patients (5 male and 5 female) ranging in age from 20 to 74 years [mean (42 ± 18) years] enrolled in experimental group. The average airway cross-sectional areas before and after treatment of 2 groups were (22 ± 13) mm(2), (23 ± 11) mm(2) (control group) and (20 ± 8) mm(2), (34 ± 12) mm(2) (experimental group), respectively. The average increased airway cross-sectional areas of experimental group and control group before and after treatment were (15 ± 13) mm(2) and (1 ± 10) mm(2) respectively, which had statistical differences. The average times of treatment during 3 months were (2.8 ± 1.5) in control group vs (1.8 ± 0.9) in experimental group which had statistical differences. The 3-month effective rate was 90% of experimental group vs 40% of control group, and the symptom improvement rate was 80% of experimental group vs 30% of control group. There were significant differences in outcomes between two groups. No drug-related complications occurred in experimental group during the follow-up period. Mitomycin-C is an effective adjuvant treatment method in the management of BCAS. Topical application of mitomycin-C in airway is safety. Catheter drip to achieve the topical application of mitomycin-C in airway is a convenient, economical and effective method.


Qi L.,Capital Medical University
Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = Chinese journal of reparative and reconstructive surgery | Year: 2010

To explore the effective surgical approaches in treating subclavian artery occlusion. Between December 2005 and February 2010, 53 patients with subclavian artery occlusion were treated, including left subclavian artery occlusion (35 cases) and stenosis (5 cases), right subclavian artery occlusion (5 cases) and stenosis (4 cases), and bilateral subclavian artery occlusion (4 cases). There were 40 males and 13 females with an average age of 64 years (range, 22-77 years), including 49 cases of arteriosclerosis obliterans and 4 cases of aortic arteritis. The disease duration was 15 days to 20 months (6.5 months on average). In 49 patients with unilateral subclavian artery occlusion, 39 cases complicated by carotid or/ and cerebral artery lesion underwent axillo-axillary bypass grafting, and 10 cases without carotid or/and cerebral artery lesion underwent carotid-subclavian bypass grafting. Ascending aorta to bi-subclavian bypass grafting were performed on 4 cases with bilateral subclavian artery occlusion. After operation, patients received routine treatment with anticoagulant and antiplatelet agents. The operations were successfully performed in 52 cases with a successful rate of 98.11%. Thrombogenesis at anastomotic site occurred in 1 case of aortic arteritis after 48 hours. Two cases had brachial plexus crush injury and 4 had hematoma around the bilateral anastomosis after axillo-axillary bypass grafting, and all recovered with nonoperative therapy. A total of 52 patients were followed up 1-52 months (24.5 months on average). All patients survived and the symptoms of basilar and upper limb artery ischemia disappeared. Doppler ultrasonography showed that the blood flow was patent through anastomosis and polytetrafluoroethylene graft, and the vertebral artery flow was normal. Pseudoaneurysm at anastomosis was found in 1 case after 18 months and treated by interventional embolization. The postoperative graft patency rate was 100% at 1 year and at 2 years. Both thoracic and extrathoracic surgical approaches are effective for treating subclavian artery occlusion. The reasonable surgical approach should be selected according to the arteriopathy and the patient's condition. Perioperative treatment and strict intraoperative manipulation are important to guarantee the success of surgery.


Chen X.,Capital Medical University
Zhongguo zhen jiu = Chinese acupuncture & moxibustion | Year: 2013

To observe the impacts of transcutaneous acupoint electric stimulation on hemodynamics, anesthetic and relevant complications in patients of transsphenoidal pituitary tumor resection (TPTR). Ninety cases of the selective TPTR were randomized into a transcutaneous acupoint electric stimulation group (group T), a sham-acupoint group (group S) and a control group (group C); 30 cases in each one. In group T, the transcutaneous acupoint electric stimulation at Hegu (LI 4), Waiguan (TE 5), Jinmen (BL 63), Taichong (LR 3), Zusanli (ST 36) and Qiuxu (GB 40). In group S, the transcutaneous acupoint electric stimulation was applied to the sham-acupoints. In group C, the acupoints selected were same as those in group T, but the electrode pads were just attached on the related points with no-electric stimulation. The electric stimulation lasted from analgesic induction till the end of the operation. The endotracheal intubation was done under the induction by propofol, fentanyl and vecuronium bromide. Propofol and remifentanil were maintained till the end of operation. Blood pressure, heart rate, bispectral index (BIS) value, anesthetic and postoperative recovery situation were recorded. The hemodynamics maintained stably in each group. In group T, the mean arterial pressure 1 min after intubation, after extubation and during directional force recovery was all lower than the other two groups respectively (all P<0.05). In group T, the effect-site concentration of fentaryl in the exposure saddle area and tumor removal stage was lower than the other two groups (all P<0.05). In 24 h after operation, 2 cases presented nausea and vomiting in group C. There were no relevant complications in the rest groups. The transcutaneous acupoint electric stimulation improves the stability of hemodynamics in perioperative stage, reduces the intraoperative opi oids dosages and improves the quality of anesthetic recovery.


Liu J.,Capital Medical University
Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese journal of integrated traditional and Western medicine / Zhongguo Zhong xi yi jie he xue hui, Zhongguo Zhong yi yan jiu yuan zhu ban | Year: 2013

To explore the efficacy of jieyu granule (JG) combined Paroxetine in treating refractory depression (RD) patients of yin deficiency inner heat syndrome (YDIHS). Seventy RD patients of YDIHS were randomly assigned to the experimental group (JG combined Paroxetine) and the control group (Chinese medical placebo combined Paroxetine), 35 cases in each group. Hamilt Depression Rating Scale and Hamilton Anxiety Scale were used before treatment, and at the weekend of the 2nd, 4th, and 8th week, respectively. In the experimental group, 32 patients completed the trial and 3 patients dropped out. In the control group, 33 patients completed the trial and 2 patients dropped out. At the end of the 8th week of the treatment, the total score of Hamilt Depression Rating Scale was (14.75 +/- 7.85) in the experimental group, lower than that of the control group (19. 06 +/- 8. 31, P <0.05). At the end of the 2nd, 4th, and 8th week of the treatment, the score of Hamilton Anxiety Scale was 17.03 +/- 4.25, 14.50 +/- 5. 13, and 11.03 +/- 4.88, respectively in the experimental group, lower than that of the control group at each corresponding time point (19. 60 +/-3. 96, 17. 12 +/- 4.14, 14.64 +/- 4.47, P <0.05, P <0.01). The efficacy of JG combined Paroxetine for treating RD patients of YDIHS was superior to that of using Paroxetine alone.


Zheng J.,Peking University | Zhu X.,Capital Medical University | Zhang J.,Peking University
Biochemical and Biophysical Research Communications | Year: 2014

CXCL5 (epithelial neutrophil activating peptide-78) which acts as a potent chemoattractant and activator of neutrophil function was reported to play a multifaceted role in tumorigenesis. To investigate the role of CXCL5 in bladder cancer progression, we examined the CXCL5 expression in bladder cancer tissues by real-time PCR and Western blot, additionally, we used shRNA-mediated silencing to generate stable CXCL5 silenced bladder cancer T24 cells and defined its biological functions. Our results demonstrated that mRNA and protein of CXCL5 is increased in human bladder tumor tissues and cell lines, down-regulation of CXCL5 in T24 cells resulted in significantly decreased cell proliferation, migration and increased cell apoptosis in vitro through Snail, PI3K-AKT and ERK1/2 signaling pathways. These data suggest that CXCL5 is critical for bladder tumor growth and progression, it may represent a potential application in cancer diagnosis and therapy. © 2014 Elsevier Inc. All rights reserved.


Wan Y.G.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To investigate the impact of prior cerebral infarction (PCI) on in-hospital mortality in patients with Acute Myocardial Infarction (AMI). A retrospective analysis of documents of a total of 3572 consecutive patients with AMI admitted to Xuanwu Hospital of Capital Medical University from 2002 Jan. 1 to 2009 Dec. 31 were performed. There were 564 patients (15.8%) with PCI. Compared with the group of without PCI, the group with PCI were substantially older [(69.4 ± 9.9) vs (64.2 ± 12.9) years, P = 0.000], and had a higher prevalence of hypertensive disease, diabetes mellitus, prior myocardial infarction (MI) and non-ST-segment elevation myocardial infarction (NSTEMI) (respectively, 71.0% vs 57.3%; 41.0% vs 25.7%, 12.9% vs 9.5%; 14.9% vs 10.7%, P < 0.01), and a higher in-hospital mortality (16.5% vs 10.0%, P = 0.000). Univariate analysis demonstrated that in-hospital mortality associated with age, gender, extensive anterior MI, anterior MI, diabetes mellitus, prior cerebral infarction, prior myocardial infarction, coronary angiography and percutaneous coronary intervention. Logistic regression analysis found that risk factors were age, extensive anterior MI, anterior MI, diabetes mellitus and prior cerebral infarction, and protective factors were coronary angiography and percutaneous coronary intervention. PCI was independently associated with in-hospital mortality, OR 1.368, 95%CI 1.047 - 1.787, P = 0.022. In patients with acute myocardial infarction, the presence of PCI increases the risk of worse in-hospital outcome.


Zhao D.,Capital Medical University
Zhonghua nei ke za zhi | Year: 2015

OBJECTIVE: To analyze the distribution and treatment of risk factors in hospitalized stroke patients before stroke onset.METHOD: This was a multi-center cross-sectional study. Patients with acute stroke were collected from 41 hospitals in 25 provinces in China from January to May in 2011.RESULTS: A total of 20 570 stoke patients (13 062 men, 7 508 women) aged (63.0 ± 12.9) years were enrolled and analyzed in this study. Among them, 15 329 were first-onset stroke, and 17 052 were ischemic stroke. (1) Of all the subjects, 75.5% were with hypertension, 53.5% with elevated LDL-C, 37.3% with diabetes, and 6.5% with atrial fibrillation. 75.2% of them had two or more above risk factors and 43.0% had three or more risk factors. (2) According to the current definition, 53.3% of the first-onset stroke patients were classified as at high risk and 25.9% were classified as at low risk. Noticeably, 42.1% of the patients below 65 years old were at low risk by the same definition. (3) The awareness rate of hypertension was 70.3% in the first-onset stroke patients. However, only 20.1% of the patients reached the target of blood pressure control in the treatment. Although the awareness rate of hypertension and diabetes among recurrent stroke patients were relatively high, the treatment and control rates of these risk factors were still low. Compared with the other two risk factors, the awareness, treatment and control rates of elevated LDL-C were much lower.CONCLUSIONS: Majority of the stroke patients are complicated with multiple risk factors before stroke onset, suggesting a great needs for improving the primary and secondary prevention of stroke in China. In addition, the definition for risk classification of stroke may need to be modified for subjects under 65 years old.


Li Y.,Harbin Medical University | Xu J.,Harbin Medical University | Chen H.,Harbin Medical University | Bai J.,Harbin Medical University | And 7 more authors.
Nucleic Acids Research | Year: 2013

Glioma is the most common and fatal primary brain tumour with poor prognosis; however, the functional roles of miRNAs in glioma malignant progression are insufficiently understood. Here, we used an integrated approach to identify miRNA functional targets during glioma malignant progression by combining the paired expression profiles of miRNAs and mRNAs across 160 Chinese glioma patients, and further constructed the functional miRNA-mRNA regulatory network. As a result, most tumour-suppressive miRNAs in glioma progression were newly discovered, whose functions were widely involved in gliomagenesis. Moreover, three miRNA signatures, with different combinations of hub miRNAs (regulations≥30) were constructed, which could independently predict the survival of patients with all gliomas, high-grade glioma and glioblastoma. Our network-based method increased the ability to identify the prognostic biomarkers, when compared with the traditional method and random conditions. Hsa-miR-524-5p and hsa-miR-628-5p, shared by these three signatures, acted as protective factors and their expression decreased gradually during glioma progression. Functional analysis of these miRNA signatures highlighted their critical roles in cell cycle and cell proliferation in glioblastoma malignant progression, especially hsa-miR-524-5p and hsamiR-628-5p exhibited dominant regulatory activities. Therefore, network-based biomarkers are expected to be more effective and provide deep insights into the molecular mechanism of glioma malignant progression. © 2013 The Author(s).


Li P.,Peking University | Luo Y.,Peking University | Chen Y.-M.,Capital Medical University
Heart Lung and Circulation | Year: 2013

Background: The use of plasma levels of B-type natriuretic peptides (BNPs) to guide treatment of patients with chronic heart failure (HF) has been investigated in a number of randomised controlled trials (RCTs). However, the benefits have been variable. We therefore performed a meta-analysis to examine the overall effect of BNP-guided drug therapy on all-cause mortality and HF rehospitalisation in patients with chronic HF. Methods: We identified RCTs by systematic search of MEDLINE, EMBASE and the Cochrane Controlled Clinical Trials Register Database. Eligible RCTs were those that enrolled more than 40 patients and involved comparison of BNP-guided versus guideline-guided drug therapy of the patients with chronic HF in the outpatient setting. Results: Eleven RCTs with a total of 2414 patients and with a mean duration of 12 months (range, 3-36 months) were included in the meta-analysis. Overall, there was a significantly decreased risk of all-cause mortality (relative risk [RR], 0.83; 95% confidence interval [CI], 0.69-0.99; P=0.035; I2=0%) and HF rehospitalisation (RR, 0.75; 95% CI, 0.62-0.91; P=0.004; I2=62.2%) in the BNP-guided therapy group. Age, baseline BNP are the major dominants of HF rehospitalisation when analysed using meta-regression. In the subgroup analysis, HF rehospitalisation was significantly decreased in the patients younger than 70 years (RR, 0.45; 95% CI, 0.33-0.61; P=0.000; I2=0.0%), or with baseline higher BNP (≥2114pg/mL) (RR, 0.53; 95% CI, 0.39-0.72; P=0.000; I2=21.8%). Conclusions: Compared with usual clinical care, B-type natriuretic peptide-guided therapy reduces all-cause mortality and HF rehospitalisation, especially in patients younger than 70 years or with higher baseline BNP. © 2013 Australian and New Zealand Society of Cardiac and Thoracic Surgeons (ANZSCTS) and the Cardiac Society of Australia and New Zealand (CSANZ).


Dong J.,University of Science and Technology Beijing | Wang Y.,University of Science and Technology Beijing | Zhang J.,University of Science and Technology Beijing | Zhan X.,Peking University | And 3 more authors.
Soft Matter | Year: 2013

Multiple stimuli-responsive polymeric micelles that can respond to light, temperature and pH have been prepared by a novel polymer, pyrene-functionalized poly (dimethylaminoethyl methacrylate), where the pyrene-quaternized segments form a light-responsive shell and the unquaternized segments form a temperature/pH-responsive core. Under UV irradiation, the micelles could be dissociated; when the temperature increased above the lower critical solution temperature, the micelles shrunk. At pH 3, the micelles could be swelled/dissociated and at pH 10, the micelles could be collapsed to complex micelles. The controlled release of Nile Red from the micelle under stimuli was demonstrated. This novel multiple stimuli-responsive micelle shows potential as a new nanocarrier and delivery system. © The Royal Society of Chemistry 2013.


Zhang M.,Capital Medical University
World Journal of Gastroenterology | Year: 2015

Helicobacter pylori (H. Pylori) infection is associated with a variety of upper gastrointestinal diseases, including gastric cancer. With the wide application of antibiotics in H. Pylori eradication treatment, drugresistant strains of H. Pylori are increasing. H. Pylori eradication treatment failure affects the outcome of a variety of diseases of the upper gastrointestinal tract. Therefore, antibiotic resistance that affects H. Pylori eradication treatment is a challenging situation for clinicians. The ideal H. Pylori eradication therapy should be safe, effective, simple, and economical. The eradication rate of triple antibiotic therapy is currently less than 80% in most parts of the world. Antibiotic resistance is the main reason for treatment failure, therefore the standard triple regimen is no longer suitable as a first-line treatment in most regions. H. Pylori eradication treatment may fail for a number of reasons, including H. Pylori strain factors, host factors, environmental factors, and inappropriate treatment. © 2015 Baishideng Publishing Group Inc. All rights reserved.


Liu H.X.,Capital Medical University
Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases] | Year: 2010

To survey the treatment status and clinical features of hospitalized patients with acute myocardial infarction (AMI) of 13 hospitals in Beijing in 2005. Uniform questionnaires were used to register AMI patients hospitalized from January 1 to December 31, 2005 in the 13 hospitals including traditional Chinese medicine (TCM) hospitals (n = 6) and western medicine hospitals (WM, n = 7) from Beijing. A total of 1663 AMI patients were registered (1366 cases in WM hospitals and 297 cases in TCM hospitals). An Access database was established and patient information was input, the clinical features and treatment status of hospitalized AMI patients were analyzed. The mean age was (63.9 +/- 12.8) years old [(62.8 +/- 12.8) years for WM Hospitals and (69.1 +/- 11.8) years for TCM hospitals, P < 0.05], male to female ratio was 2.4:1 (2.7:1 for WM hospitals and 1.6:1 for TCM hospitals, P < 0.05). The median time to hospital was 14 hours in TCM hospitals and 11 hours in WM hospitals (P > 0.05). Incidences of history of cerebrovascular disease, high blood pressure, diabetes, hyperlipidemia and complications such as in-patient arrhythmia, cardiac insufficiency, cardiogenic shock were significantly higher in TCM hospitals than in WM hospitals. The total mortality of 1663 AMI cases was 8.2% (15.8% in TCM hospitals vs. 6.6% in WM hospitals, P < 0.01). The reperfusion rate including emergency PCI and thrombolytic therapy rate was 31.3% in 13 hospitals (33.3% in WM hospitals vs. 21.9% in TCM hospitals, P < 0.05). Percent of guideline recommend drug use for AMI was as follows: aspirin 93.6%, ACEI and ARB 85.1%, beta-blocker 78.7%, low molecular weight heparin 85.4%, statins 74.7%. Reperfusion therapy and guideline recommended drugs were widely used although there was a need for further improvement. The hospitalized mortality showed a downward trend compared with results from five years ago, patients in TCM hospitals had an independent clinical features.


Li Y.,Capital Medical University
Lin chuang er bi yan hou tou jing wai ke za zhi = Journal of clinical otorhinolaryngology, head, and neck surgery | Year: 2010

To discuss the perioperative complications of 1396 cases (1402 ears) with cochlear implantation (CI), and to supply clinical experience for risk reduction in the perioperative period. All patients were profound sensorineural deafness, with 1379 prelingual cases and 17 postlingual cases. (1) Preoperative examinations: audiology,imaging studies, evaluation of physical and intellectual development. (2) Perioperative complications. (1) Perioperative complications: transitory postoperative facial palsy in 4 cases; external auditory canal and membrana tympani injury in 14 cases; gusher in 91 cases; cerebral dura mater injury in 2 cases; problems of electrodes in 31 cases including 2 of electrodes inserted into inner auditory canal and 1 into scala vestibuli, 28 of electrodes squire in the cochlea; transient vertigo in 231 cases; scalp hematoma in 39 cases. (2) Systematic postoperative complications: fever ( >38 degrees C) in 21 cases; acute gastritis in 27 cases; bronchitis and pneumonia in 5 cases; laryngotracheitis in 9 cases. (3) Other complications: artificial cochlear exchange due to computer trouble during operation. (1) CI is a relatively safe surgical procedure, but risks still persist during perioperative period including postoperative meningitis, facial nerve and chorda tympani nerve injury,and so on. (2) To ensure successful operation, perioperative managements should be standardized.


WANG J.,Capital Medical University
Zhonghua er bi yan hou tou jing wai ke za zhi = Chinese journal of otorhinolaryngology head and neck surgery | Year: 2012

To verify the correlation between nasal contact point and headache and to discuss the significance of nasal endoscopic surgery through observation of clinical outcomes in patients with nasal mucosa contact point treated by endoscopic surgery. Forty-five patients diagnosed as nasal mucosa contact headache were treated by nasal endoscopic surgery. The results were analyzed retrospectively, including headache degree, headache frequency, lasting time and total time between before and 6, 18, and 24 months after operation. The data were processed by rank-sum test by SPSS 18.0 software. 6, 18 and 24 months after operation, the headache degree (VAS scores were 1.50 [0; 4.00], 2.00 [0; 5.00], 3.25 [0; 5.75], respectively) was relieved (VAS score was 6.00 [5.25; 8.25] before operation) dramatically (Z value were -4.913, -4.070 and -3.095, respectively, all P < 0.01). The total time of headache 6, 18, 24 months after operation (It were 0.07 [0; 3.50], 0.26 [0; 7.77], 1.04[0; 17.15] h, respectively) was shortened (It was 25.20 [4.00; 186.00] h before operation) significantly (Z value were -4.368, -3.652, -2.500, respectively, all P < 0.05). One of the key causes of patients suffered from intractable headaches is mucosal contact in the nasal cavity. The pain in these patients could be relieved through surgical correction of intranasal anatomic abnormalities. Nasal mucosa contact might not be the only etiology of intractable headache since the mechanism of headache is complicated and variable. The effect of endoscopic surgery needs to be estimated by long-term follow-up.


Ren J.,Capital Medical University
Zhonghua xin xue guan bing za zhi | Year: 2010

To evaluate the relationship between serum non-HDL-C and incidence of various cardiovascular disease (CVD) in Chinese population aged 35-64 years. This prospective study was performed from 1992 to 2004 in 11 provinces of China and the association between baseline non-HDL-C level with the risk of various CVD events was analyzed in 29 937 subjects aged 35-64 years using Cox multivariate proportional hazards regression. CVD events in this study including acute coronary events (ACE), ischemic stroke, hemorrhagic stroke and ischemic cardiovascular disease (ICVD). (1) Adjusted for age, gender, smoking status, diabetes, body mass index and blood pressure, the relative risk of ACE, ischemic stroke and ICVD in groups of non-HDL-C 3.37 - 4.13 mmol/L (130 - 159 mg/dl), 4.14 - 4.91 mmol/L (160 - 189 mg/dl) and ≥ 4.92 mmol/L (190 mg/dl) was 1.24 (0.91 - 1.70), 1.78 (1.25 - 2.53), 2.23 (1.48 - 3.35); 1.34 (1.07 - 1.68), 1.38 (1.04 - 1.83), 1.38 (0.97 - 1.94) and 1.37 (1.12 - 1.63), 1.52 (1.22 - 1.90), 1.70 (1.30 - 2.22), respectively. The risk of hemorrhagic stroke was declined obviously in group > 4.92 mmol/L (190 mg/dl). (2) The correlation between VLDL-C and ACE was the strongest in four CVD events when VLDL-C and LDL-C were joint analyzed, ICVD events ranked the second. The risk for ischemic stroke also borderline increased with increasing VLDL-C and LDL-C (P > 0.05). Increased non-HDL-C is associated with increased risk of suffering ACE, ischemic stroke and ICVD and VLDL-C plays a critical role in the development of ICVD events, especially ACS, in middle aged Chinese population.


Xing T.Y.,Capital Medical University
Sheng li ke xue jin zhan [Progress in physiology] | Year: 2012

Recently, 5-hydroxymethyl cytosine (5-hmC) has been discovered as a naturally existed component of normal mammalian genomic DNAs, and it is generally accepted as the sixth base in the genome. This review will introduce the recent advances in the researches on 5-hmC of its formation, tissue-specific distribution, the roles in cell differentiation and gene expression regulation, and the connections as a epigenetic marker with diseases, such as various cancers. We also summarized the current development of the methodologies to detect methylated or hydroxymetholated cytosines of cells at the genomic levels.


To survey the incidence of lower urinary tract symptoms (LUTS) and erectile dysfunction (ED) in males aged ≥ 50 years and explore the correlation between LUTS and ED. A cross-sectional study was performed at Beijing communities in 1644 males aged over 50 years. The International Index of Erectile Function-5 (IIEF-5) and International Prostate Symptom Score (IPSS) were recorded. Pearson's χ(2) test and Spearman correlation coefficients were used to analyze the results of IPSS, LUTS and their correlations with ED. The mean age was 64.5 years old (range: 50-93), the mean value of IPSS(9.9 ± 8.2), the prevalence of mild, moderate and severe LUTS 49.2% (809/1644), 36.4% (599/1644) and 14.4% (236/1644) respectively. The mean value of IIEF was (9.4 ± 8.6), the total incidence of ED 90.5% (1487/1644) and the incidence of ED of mild, moderate and severe LUTS 85.7% (694/809), 93.7% (561/599) and 97.9% (231/236) respectively. The total IIEF-5 score was found significantly correlated with the total IPSS score (r = -0.335, P < 0.01), the obstructive symptoms (r = -0.276, P < 0.01)and irritative symptoms (r = -0.326, P < 0.01). The severity of LUTS was correlated with the severity of ED (r = 0.304, P < 0.01). Correlations also existed between age and total IPSS score(r = 0.388, P < 0.01), LUTS severity (r = 0.457, P < 0.01), total IIEF score (r = -0.533, P < 0.01) or ED severity (r = 0.529, P < 0.01). The incidence of LUTS or ED in aging males increases with age. The severity of ED is positively correlated with the severity of LUTS. Irritative and obstructive symptoms influence the occurrence of ED in aging males.


Gao Y.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To investigate the characteristics of gastroesophageal reflux disease (GERD) with chronic cough by the results of combined multichannel intraluminal impedance and high-resolution manometry (MII-HRM) procedure and 24-hour multichannel intraluminal impedance combined pH (MII-pH) monitoring. From March 2010 to November 2010, consecutive patients of GERD with chronic cough (more than 8 weeks) admitted to Beijing Chaoyang Hospital of Capital Medical University underwent 24-hour MII-pH monitoring and MII-HRM procedure with symptom association probability (SAP) over 95%. Data of lower esophageal sphincter (LES) pressure, LES relaxation, LES residual pressure, esophageal body peristalsis function and swallow bolus transit (liquid/viscous) was collected and the result of 24-hour MII-pH monitoring was analysed by the computer software containing reflux episode activity (acid/nonacid, upright/recumbent), proximal extent, acid exposure and mean acid/bolus clearance time. Seventeen patients of GERD with typical reflux symptom were selected as the control group. Comparing with patients of GERD with typical reflux symptom, patients of GERD with chronic cough showed decreased upper esophageal sphincter pressure (UESP) [(122.55 ± 60.48) mm Hg vs (86.37 ± 41.35) mm Hg, P < 0.05, 1 mm Hg = 0.133 kPa], higher percentage of abnormality peristalsis [(9.47 ± 15.63)% vs (22.16 ± 17.45)%, P < 0.05], degraded esophagus transmittability of liquid substance [(88.82 ± 12.23)% vs (71.68 ± 23.06)%, P < 0.05], more reflux episode activity (acid/nonacid) in supine position and proximal reflux episode (nonacid) in supine position (P < 0.05) and longer mean bolus clearance time (P < 0.05). Decrease of the UESP, increase of the reflux episode activity (acid/nonacid) in supine position and proximal reflux episode (nonacid) in supine position, lengthened mean bolus clearance time in supine position and the degraded esophagus clearance ability may all correlated with the pathogenesy of GERD with chronic cough.


Gish R.,University of California at San Diego | Jia J.-D.,Capital Medical University | Locarnini S.,Victorian Infectious Diseases Reference Laboratory | Zoulim F.,French Institute of Health and Medical Research | Zoulim F.,Institut Universitaire de France
The Lancet Infectious Diseases | Year: 2012

Antiviral drug resistance is a crucial factor that frequently determines the success of long-term therapy for chronic hepatitis B. The development of resistance to nucleos(t)ide analogues has been associated with exacerbations in liver disease and increased risk of emergence of multidrug resistance. The selection of a potent nucleos(t)ide analogue with a high barrier to resistance as a first-line therapy, such as entecavir or tenofovir, provides the best chance of achieving long-term treatment goals and should be used wherever possible. The barrier to resistance of a given nucleos(t)ide analogue is influenced by genetic barrier, drug potency, patient adherence, pharmacological barrier, viral fitness, mechanism of action, and cross-resistance. In countries with limited health-care resources, the selection of a therapy with a high barrier to resistance is not always possible and alternative strategies for preventing resistance might be needed, although limited data are available to support these strategies. © 2012 Elsevier Ltd.


Wang H.,Harbin Medical University | Wang Y.,Capital Medical University | Jiang C.,Harbin Medical University
International Journal of Oncology | Year: 2013

To explore the expression pattern, prognostic value and functional role of stromal periostin (POSTN) in glioma patients, POSTN expression was measured using the Agilent Whole Human Genome Oligo Microarray in 220 frozen glioma tissues. We analyzed POSTN expression in 71 independent validated glioma samples using immunohistochemistry. The expression levels of POSTN were relative to glioma grade progression and inversely correlated with overall survival in high-grade glioma patients (anaplastic gliomas and glioblastomas). Gene ontology (GO) analysis performed using DAVID showed that the gene sets related to cell migration and proliferation were significantly enriched in the cases with POSTN overexpression. Functional analyses in LN229 and U87 cells revealed that POSTN was involved in cell invasion and proliferation. MMP-9 was an effector of POSTN signaling in glioma cells. The expression of stromal protein POSTN is relative to glioma grade progression and confers a poor prognosis via promoting cellular invasion and proliferation in high-grade glioma patients.


Xia K.,Capital Medical University
Zhonghua nei ke za zhi [Chinese journal of internal medicine] | Year: 2011

To compare the safety and efficacy of radial artery access versus femoral artery access for percutaneous coronary intervention in acute myocardial infarction population. From June 2004 to December 2006, 446 patients with acute myocardial infarction treated with percutaneous stenting were reviewed retrospectively. The radial artery approach was used in 242 patients, and the femoral artery approach in 204 patients. The success of the procedure, procedure duration, X-ray exposition, volume of contrast, incidence of major adverse cardiac events and complications were compared between the radial artery and femoral artery approach. Total procedure duration, X-ray exposition, the immediate success of the procedure and the proportion of patients with reperfusion time above 60min are higher in patients with radial artery acess than that with femoral artery access [(62.1 ± 23.4) min vs (56.8 ± 16.7) min, (2829.4 ± 1365.2) mGY vs (2352.3 ± 903.1) mGY, 4% vs 0.9% and 7.44% vs 2.94% respectively, all P < 0.05]. In non-selected patients with acute myocardial infarction treated with primary stent implantation, the success rate of the radial artery approach is lower than the femoral artery approach and could prolong the reperfusion time. It is suitable to change artery access immediately if abnormality is found via radial artery access.


Gu H.F.,Karolinska University Hospital | Ma J.,Capital Medical University | Gu K.T.,Viktor Rydberg Gymnasium Odenplan School | Brismar K.,Karolinska University Hospital
Frontiers in Endocrinology | Year: 2013

Diabetes and diabetic nephropathy are complex diseases affected by genetic and environmental factors. Identification of the susceptibility genes and investigation of their roles may provide useful information for better understanding of the pathogenesis and for developing novel therapeutic approaches. Intercellular adhesion molecule 1 (ICAM1) is a cell surface glycoprotein expressed on endothelial cells and leukocytes in the immune system. The ICAM1 gene is located on chromosome 19p13 within the linkage region of diabetes. In the recent years, accumulating reports have implicated that genetic polymorphisms in the ICAM1 gene are associated with diabetes and diabetic nephropathy. Serum ICAM1 levels in diabetes patients and the icaml gene expression in kidney tissues of diabetic animals are increased compared to the controls. Therefore, ICAM1 may play a role in the development of diabetes and diabetic nephropathy. In this review, we present genomic structure, variation, and regulation of the ICAM1 gene, summarized genetic and biological studies of this gene in diabetes and diabetic nephropathy and discussed about the potential application using ICAM1 as a biomarker and target for prediction and treatment of diabetes and diabetic nephropathy. © 2013 Gu, Ma, Gu and Brismar.


Xiao D.,China Medical University at Heping | Liu H.,Shenyang University | Zhang H.,China Medical University at Heping | Luo Y.,Capital Medical University
Journal of Neurology | Year: 2012

Studies have indicated that serum levels of cystatin C (a sensitive marker of renal function) are significantly associated with cerebral vascular events. However, the influence of cystatin C on infarct size and hemorrhage volume in acute cerebral stroke has not been well established. A total of 222 patients with cerebral infarction, and 69 patients with cerebral hemorrhage, as well as 122 healthy controls were included in this study. Patients were further divided into subgroups according to infarct size and hemorrhage volume. Serum levels of cystatin C were significantly higher in cerebral-stroke patients than healthy controls (p<0.05). Logistic multiple regression analyses showed that cystatin C levels were correlated with ischemic stroke and hemorrhagic cerebral stroke (p<0.01). Cystatin C levels were correlated only with age, urea level, and creatinine level (p<0.05). There was no correlation between cystatin C levels and systolic blood pressure, diastolic blood pressure, as well as levels of fasting blood glucose, triglyceride, total cholesterol, lowdensity lipoprotein cholesterol, and high-density lipoprotein cholesterol (p[0.05). Patients with larger infarcts or larger hemorrhage volumes had higher levels of cystatin C (p<0.05). Certain factors affect cystatin C levels in cerebral-stroke patients, and they could be considered to be independent predictors of infarct size and hemorrhage volume in acute cerebral stroke events. © Springer-Verlag 2012.


Zhang M.,Capital Medical University
National Medical Journal of China | Year: 2016

Objective: To study the information processing speed and the influential (actors in multiple sclerosis (MS) patients. Methods: A total of 36 patients with relapsing-remitting MS (RRMS) , 21 patients with secondary progressive MS (SPMS) , and 50 healthy control subjects from Xuanwu Hospital of Capilal Medical University between April 2010 and April 2012 were included into this cross-sectional study, Neuropsvchological tests was conducted after the disease had been stable for 8 weeks, including information processing speed, memory, executive functions, language and visual perception. Correlation between information processing speed and depression, fatigue, Expanded Disability Status Scale (EDSS) were studied. Results: (1) MS patient groups demonstrated cognitive deficits compared to healthy controls. The Symbol Digit Modalities Test (SDMT) (control group 57 ± 12; RRMS group 46 ± 17; SPMS group 35 ± 10, P<0. 05) and Paced Auditory Serial Addition Task (PASAT) (control group 85 ± 18; RRMS group 77 ±20; SPMS group 57 ±20, P < 0. 05) impaired most. SPMS patients were more affected compared to patients with RRMS subtypes, and these differences were attenuated alter control for physical disability level as measured by the EDSS scores. MS patients, especially SPMS subtype, were more severely impaired than control group in the verbal learning lest, verbal fluency, Stroop C test planning time, while visual-spatial lunction and visual memory were relatively reserved in MS patients. (2) According to the Pearson univariate correlation analysis, age, depression, EDSS scores and fatigue were related with PASAT and SDMT tests (r = -0. 41 - - 0. 61 , P <0. 05). Depression significantly affected the speed of information processing (P<0. 05). Conclusions: Impairment of information processing speed, verbal memory and executive functioning are seen in MS patients, especially in SPMS subtype, while visual-spatial function is relatively reserved. Age, white matter change scales, EDSS scores, depression are negatively associated with information processing speed.


Objective This study aimed to evaluate the safety and efficacy of preoperative induction therapy using a single high dose (9 mg/kg) of antithymocyte globulin-Fresenius S (ATG-F) for patients undergoing renal transplantation. Methods Randomized controlled clinical trials (RCTs) on the safety and efficacy of preoperative induction therapy using a single high dose of ATG-F for patients undergoing renal transplantation were searched in Cochrane Library, PubMed, FMBASE covering a period from the beginning of databases to July 2015. The meta-analysis was conducted using RevMan 5. 2. Results Five RCTs with 346 patients were included in this study. The meta-analysis showed that the incidences of acute rejection for the patients with renal transplantation were 20. 6% (37/180) in the induction therapy group using a single high dose of ATG and 42. 8% (71/166) in the control group, with a combined relative risk (RR) of 0. 49 and 95% confidence interval (67) of'L0. 36, 0. 69] (P <0. 000 1). The patient survival rate (1 year; RR = 1.02, 95% 67[0.98, 1.06], P=0.43; 5 years; RR = \.0\, 95% 67[0.94, 1.08], P=0. 83) and the graft survival rate (RR = 1. 04, 95% CI [0.91, 1. 12], P = 0. 24) of the two groups were similar. The incidences of CMV infection, urinary tract infection, and malignant tumor were also similar in the two groups. Conclusion The induction therapy using a single high dose of ATG-F significantly reduced the incidence of acute rejection after transplantation and showed no increased incidence of urinary tract infection, CMV infection, or malignant tumor. The results of our meta-analysis suggest that the application of a high dose of ATG-F may be a safe and effective induction therapy.


Wang J.-W.,Capital Medical University | Hu D.-Y.,Peking University
Journal of the American Society of Hypertension | Year: 2015

Passive smoking is now recognized to be associated with early arterial damage. The aim of this study was to assess the relationship between secondhand smoke (SHS) exposure, measured objectively by serum cotinine level, and the parameters used to assess vascular structure and function among never smokers in North China. From January 2008 to August 2008, 652 adults aged 20-70 years were enrolled. Brachial-ankle pulse wave velocity (baPWV), ankle-brachial index, and carotid intima-media thickness measurements were performed in all patients. All participants were required to respond to an interviewer-led questionnaire including medical histories and demographic data and to receive blood tests on biochemical indicators. We found that in nonsmokers, higher levels of serum cotinine were positively associated with higher baPWV and brachial pulse pressure after adjusting for heart rate, body mass index, and other confounders. Tests for linear trends for this association were statistically significant. In contrast, no association was present with ankle-brachial index and carotid intima-media thickness. In never smokers, higher SHS exposure measured objectively by serum cotinine levels was found to be associated with brachial pulse pressure and baPWV after adjusting for confounders. © 2015 American Society of Hypertension. All rights reserved.


OBJECTIVE:: The purpose of this study was to compare the ligation of intersphincteric fistula tract (LIFT) with an additional plug (LIFT-plug) in the treatment of transsphincteric anal fistula. SUMMARY BACKGROUND DATA:: Both LIFT and LIFT-plug are recently reported effective alternatives of transsphincteric anal fistula. METHODS:: This multicenter prospective randomized study (NCT01478139) was conducted at 5 university hospitals throughout China. A total of 235 patients were randomly assigned to undergo LIFT (118 patients) or LIFT-plug (117 patients) between March 2011 and April 2013. The primary outcome measured was primary healing rate at 6 months postoperatively and healing time. Secondary outcomes included recurrence rate, postoperative pain, and incontinence rate. RESULTS:: The LIFT procedure showed shorter operative time than the LIFT-plug procedure (26.7?min vs 28.5?min, P?=?0.03). Median healing time was 22 days in LIFT-plug group vs 30 days in LIFT group (P?


Zhao P.,Liver Failure Therapy and Research Center | Wang C.,Intensive Care Unit | Liu W.,Academy of Military Medical Science | Wang F.,Capital Medical University
Critical Care Medicine | Year: 2014

Objectives: Up to now, limited cases with acute liver failure caused by traditional Chinese medicine have been reported, and thus this topic has been scarcely discussed. This study aims to report such cases from China. Design: A retrospective study. Setting: Clinical investigation among seven tertiary hospitals in different areas of China. Patients: From January 2007 to December 2012, patients with acute liver failure induced by traditional Chinese medicinal herbs were included. Interventions: None. Meaurements and main results: A total of 30 patients were finally identified, including six men and 24 women. The average age was 39.7 years. The median period from initial symptoms to the development of hepatic encephalopathy was 13 days. Nine patients (30%) had accepted herbal therapies due to their skin disorders before the onset of acute liver failure. Eighteen patients (60%) eventually died, 10 of whom died of heavy bleeding. No patients received liver transplantation. Conclusions: The model of safety monitoring for traditional Chinese materia medica should be established. For those critically ill patients with herb-induced acute liver failure, coagulopathy is a vital problem in critical care. Additionally, the rate of liver transplantation for acute liver failure in China needs to be improved. © 2013 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins.


Zhou Y.,Capital Medical University
Zhonghua Shiyan Yanke Zazhi/Chinese Journal of Experimental Ophthalmology | Year: 2016

Safety, efficacy and predictability of corneal refractive surgery have greatly improved as the advancement of the operating technology and equipment. Laser corneal refractive surgeries include excimer laser photorefractive keratectomy (PRK), laser-assisted subepithelial keratomileusis (LASEK), laser in situ keratomileusis (LASIK) and small incision lenticule extraction (SMILE). No matter what kind of operation method,its ultimate goal is to increase the comfort level and improve visual quality. With the surgery skills improving, retinal drug use is a primary factor affecting the safety of laser corneal refractive surgery. Ophthalmologists should strictly master indications and administration of commonly used drugs in postoperative eyes to prevent drug-related eye diseases. In addition,eye doctors also should understand the postoperative complications and topical administration of eye drops. It is important to pay attention to the standard surgical operation and rational drug use after corneal refractive surgery, which can enhance the visual quality and comfort and reduce the complication. Copyright © 2016 by the Chinese Medical Association.